Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries
It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berrie...
Ausführliche Beschreibung
Autor*in: |
Xing, Ran-Ran [verfasserIn] |
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Format: |
Artikel |
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Sprache: |
Englisch |
Erschienen: |
2015 |
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Schlagwörter: |
Glucosyltransferases - genetics |
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Übergeordnetes Werk: |
Enthalten in: Journal of agricultural and food chemistry - Columbus, Ohio : American Chemical Soc., 1953, 63(2015), 12, Seite 3251 |
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Übergeordnetes Werk: |
volume:63 ; year:2015 ; number:12 ; pages:3251 |
Links: |
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Katalog-ID: |
OLC1963010272 |
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245 | 1 | 0 | |a Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries |
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520 | |a It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions. | ||
650 | 4 | |a Glucosyltransferases - genetics | |
650 | 4 | |a Plant Extracts - chemistry | |
650 | 4 | |a Plant Proteins - genetics | |
650 | 4 | |a Anthocyanins - chemistry | |
650 | 4 | |a Glucosides - chemistry | |
650 | 4 | |a Plant Proteins - metabolism | |
650 | 4 | |a Glucosides - biosynthesis | |
650 | 4 | |a Vitis - chemistry | |
650 | 4 | |a Anthocyanins - biosynthesis | |
650 | 4 | |a Vitis - classification | |
650 | 4 | |a Vitis - enzymology | |
650 | 4 | |a Glucosyltransferases - metabolism | |
650 | 4 | |a Plant Extracts - biosynthesis | |
650 | 4 | |a Vitis - genetics | |
700 | 1 | |a Li, Si-Yu |4 oth | |
700 | 1 | |a He, Fei |4 oth | |
700 | 1 | |a Yang, Zhe |4 oth | |
700 | 1 | |a Duan, Chang-Qing |4 oth | |
700 | 1 | |a Li, Zheng |4 oth | |
700 | 1 | |a Wang, Jun |4 oth | |
700 | 1 | |a Pan, Qiu-Hong |4 oth | |
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PQ20160617 (DE-627)OLC1963010272 (DE-599)GBVOLC1963010272 (PRQ)p832-7c22c84e78a59f1d29b981682234a7edae0dff681e36e3a72f5d6c4a4cda506e0 (KEY)0011841920150000063001203251massspectrometricandenzymaticevidenceconfirmtheexi DE-627 ger DE-627 rakwb eng 630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Xing, Ran-Ran verfasserin aut Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions. Glucosyltransferases - genetics Plant Extracts - chemistry Plant Proteins - genetics Anthocyanins - chemistry Glucosides - chemistry Plant Proteins - metabolism Glucosides - biosynthesis Vitis - chemistry Anthocyanins - biosynthesis Vitis - classification Vitis - enzymology Glucosyltransferases - metabolism Plant Extracts - biosynthesis Vitis - genetics Li, Si-Yu oth He, Fei oth Yang, Zhe oth Duan, Chang-Qing oth Li, Zheng oth Wang, Jun oth Pan, Qiu-Hong oth Enthalten in Journal of agricultural and food chemistry Columbus, Ohio : American Chemical Soc., 1953 63(2015), 12, Seite 3251 (DE-627)129602795 (DE-600)241619-0 (DE-576)015096610 0021-8561 nnns volume:63 year:2015 number:12 pages:3251 http://www.ncbi.nlm.nih.gov/pubmed/25771698 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_2006 GBV_ILN_4219 58.27 AVZ 58.34 AVZ 42.63 AVZ AR 63 2015 12 3251 |
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PQ20160617 (DE-627)OLC1963010272 (DE-599)GBVOLC1963010272 (PRQ)p832-7c22c84e78a59f1d29b981682234a7edae0dff681e36e3a72f5d6c4a4cda506e0 (KEY)0011841920150000063001203251massspectrometricandenzymaticevidenceconfirmtheexi DE-627 ger DE-627 rakwb eng 630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Xing, Ran-Ran verfasserin aut Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions. Glucosyltransferases - genetics Plant Extracts - chemistry Plant Proteins - genetics Anthocyanins - chemistry Glucosides - chemistry Plant Proteins - metabolism Glucosides - biosynthesis Vitis - chemistry Anthocyanins - biosynthesis Vitis - classification Vitis - enzymology Glucosyltransferases - metabolism Plant Extracts - biosynthesis Vitis - genetics Li, Si-Yu oth He, Fei oth Yang, Zhe oth Duan, Chang-Qing oth Li, Zheng oth Wang, Jun oth Pan, Qiu-Hong oth Enthalten in Journal of agricultural and food chemistry Columbus, Ohio : American Chemical Soc., 1953 63(2015), 12, Seite 3251 (DE-627)129602795 (DE-600)241619-0 (DE-576)015096610 0021-8561 nnns volume:63 year:2015 number:12 pages:3251 http://www.ncbi.nlm.nih.gov/pubmed/25771698 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_2006 GBV_ILN_4219 58.27 AVZ 58.34 AVZ 42.63 AVZ AR 63 2015 12 3251 |
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PQ20160617 (DE-627)OLC1963010272 (DE-599)GBVOLC1963010272 (PRQ)p832-7c22c84e78a59f1d29b981682234a7edae0dff681e36e3a72f5d6c4a4cda506e0 (KEY)0011841920150000063001203251massspectrometricandenzymaticevidenceconfirmtheexi DE-627 ger DE-627 rakwb eng 630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Xing, Ran-Ran verfasserin aut Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions. Glucosyltransferases - genetics Plant Extracts - chemistry Plant Proteins - genetics Anthocyanins - chemistry Glucosides - chemistry Plant Proteins - metabolism Glucosides - biosynthesis Vitis - chemistry Anthocyanins - biosynthesis Vitis - classification Vitis - enzymology Glucosyltransferases - metabolism Plant Extracts - biosynthesis Vitis - genetics Li, Si-Yu oth He, Fei oth Yang, Zhe oth Duan, Chang-Qing oth Li, Zheng oth Wang, Jun oth Pan, Qiu-Hong oth Enthalten in Journal of agricultural and food chemistry Columbus, Ohio : American Chemical Soc., 1953 63(2015), 12, Seite 3251 (DE-627)129602795 (DE-600)241619-0 (DE-576)015096610 0021-8561 nnns volume:63 year:2015 number:12 pages:3251 http://www.ncbi.nlm.nih.gov/pubmed/25771698 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_2006 GBV_ILN_4219 58.27 AVZ 58.34 AVZ 42.63 AVZ AR 63 2015 12 3251 |
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PQ20160617 (DE-627)OLC1963010272 (DE-599)GBVOLC1963010272 (PRQ)p832-7c22c84e78a59f1d29b981682234a7edae0dff681e36e3a72f5d6c4a4cda506e0 (KEY)0011841920150000063001203251massspectrometricandenzymaticevidenceconfirmtheexi DE-627 ger DE-627 rakwb eng 630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Xing, Ran-Ran verfasserin aut Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions. Glucosyltransferases - genetics Plant Extracts - chemistry Plant Proteins - genetics Anthocyanins - chemistry Glucosides - chemistry Plant Proteins - metabolism Glucosides - biosynthesis Vitis - chemistry Anthocyanins - biosynthesis Vitis - classification Vitis - enzymology Glucosyltransferases - metabolism Plant Extracts - biosynthesis Vitis - genetics Li, Si-Yu oth He, Fei oth Yang, Zhe oth Duan, Chang-Qing oth Li, Zheng oth Wang, Jun oth Pan, Qiu-Hong oth Enthalten in Journal of agricultural and food chemistry Columbus, Ohio : American Chemical Soc., 1953 63(2015), 12, Seite 3251 (DE-627)129602795 (DE-600)241619-0 (DE-576)015096610 0021-8561 nnns volume:63 year:2015 number:12 pages:3251 http://www.ncbi.nlm.nih.gov/pubmed/25771698 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_2006 GBV_ILN_4219 58.27 AVZ 58.34 AVZ 42.63 AVZ AR 63 2015 12 3251 |
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Xing, Ran-Ran |
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Xing, Ran-Ran ddc 630 bkl 58.27 bkl 58.34 bkl 42.63 misc Glucosyltransferases - genetics misc Plant Extracts - chemistry misc Plant Proteins - genetics misc Anthocyanins - chemistry misc Glucosides - chemistry misc Plant Proteins - metabolism misc Glucosides - biosynthesis misc Vitis - chemistry misc Anthocyanins - biosynthesis misc Vitis - classification misc Vitis - enzymology misc Glucosyltransferases - metabolism misc Plant Extracts - biosynthesis misc Vitis - genetics Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries |
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630 640 540 DNB 58.27 bkl 58.34 bkl 42.63 bkl Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries Glucosyltransferases - genetics Plant Extracts - chemistry Plant Proteins - genetics Anthocyanins - chemistry Glucosides - chemistry Plant Proteins - metabolism Glucosides - biosynthesis Vitis - chemistry Anthocyanins - biosynthesis Vitis - classification Vitis - enzymology Glucosyltransferases - metabolism Plant Extracts - biosynthesis Vitis - genetics |
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Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries |
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Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries |
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mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-o-diglucosides in cabernet sauvignon (vitis vinifera l.) grape berries |
title_auth |
Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries |
abstract |
It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions. |
abstractGer |
It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions. |
abstract_unstemmed |
It has been widely accepted that anthocyanidin 3,5-O-diglucosides do not exist in Vitis vinifera L. Cabernet Sauvignon (CS) berries. However, our anthocyanin analyses using HPLC-ESI-MS/MS detected the existence of a low level of anthocyanidin 3,5-O-diglucosides in the Cabernet Sauvignon grape berries grown in China. The authenticity of these samples was confirmed with microsatellite markers. The existence of anthocyanidin 3,5-O-diglucoside was further verified by the enzymatic evidence for the first time. Four putative 5-O-glucosyltransferase (5GT) genes were isolated from the Cabernet Sauvignon berries. The enzymatic analysis showed that a recombinant protein (designated as Vv5GT3) glucosylated the 3-O- and 5-O-positions of anthocyanidins and flavonols. A phylogenetic analysis revealed that this bifunctional enzyme belongs to the 5GT subfamily of UDP-glycosyltransferases. This finding brought a new understanding of the anthocyanins' profile and their biosynthesis in V. vinifera and would be helpful for further investigations of the mechanism of accumulation of anthocyanidin diglucosides in Cabernet Sauvignon berries in China's wine-producing regions. |
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container_issue |
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title_short |
Mass spectrometric and enzymatic evidence confirm the existence of anthocyanidin 3,5-O-diglucosides in cabernet sauvignon (Vitis vinifera L.) grape berries |
url |
http://www.ncbi.nlm.nih.gov/pubmed/25771698 |
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