A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation

Radiation-induced bystander effects have been observed in vitro and in cell and tissue culture models, however, there are few reported studies showing these effects in vivo. To our knowledge, this is the first reported study on bystander effects induced by microbeam irradiation in an intact living m...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	M. Buonanno [verfasserIn] G. Randers-Pehrson L. B. Smilenov N. J. Kleiman E. Young B. Ponnayia D. J. Brenner

Format:	Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Keratinocytes - radiation effects Ear - radiation effects Gene Expression - radiation effects Histones - biosynthesis DNA Damage - radiation effects

Systematik:	WA 15000

Übergeordnetes Werk:	Enthalten in: Radiation research - Lawrence, Kan. : Soc., 1954, 184(2015), 2, Seite 219-225
Übergeordnetes Werk:	volume:184 ; year:2015 ; number:2 ; pages:219-225

Links:	Volltext Link aufrufen Link aufrufen Link aufrufen

DOI / URN:	10.1667/RR14057.1

Katalog-ID:	OLC1963106989

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520			\|a Radiation-induced bystander effects have been observed in vitro and in cell and tissue culture models, however, there are few reported studies showing these effects in vivo. To our knowledge, this is the first reported study on bystander effects induced by microbeam irradiation in an intact living mammal. The mouse ear was used to investigate radiation-induced bystander effects in keratinocytes, utilizing a 3 MeV proton microbeam (LET 13.1 keV/μm) with a range in skin of about 135 μm. Using a custom-designed holder, the ear of an anesthetized C57BL/6J mouse was flattened by gentle suction and placed over the microbeam port to irradiate cells along a 35 μm wide, 6 mm long path. Immunohistochemical analysis of γ-H2AX foci formation in tissue sections revealed, compared to control tissue, proton-induced γ-H2AX foci formation in one of the two epidermal layers of the mouse ear. Strikingly, a higher number of cells than expected showed foci from direct irradiation effects. Although the proton-irradiated line w...
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allfieldsSound	10.1667/RR14057.1 doi PQ20160617 (DE-627)OLC1963106989 (DE-599)GBVOLC1963106989 (PRQ)b2340-ee919d130d2dbc7fcc5329d50070ce44bc3d4f0de8fae39032814e8c5274485c0 (KEY)0012922420150000184000200219mouseearmodelforbystanderstudiesinducedbymicrobeam DE-627 ger DE-627 rakwb eng 610 530 DNB WA 15000 AVZ rvk 44.64 bkl 44.40 bkl 33.30 bkl M. Buonanno verfasserin aut A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Radiation-induced bystander effects have been observed in vitro and in cell and tissue culture models, however, there are few reported studies showing these effects in vivo. To our knowledge, this is the first reported study on bystander effects induced by microbeam irradiation in an intact living mammal. The mouse ear was used to investigate radiation-induced bystander effects in keratinocytes, utilizing a 3 MeV proton microbeam (LET 13.1 keV/μm) with a range in skin of about 135 μm. Using a custom-designed holder, the ear of an anesthetized C57BL/6J mouse was flattened by gentle suction and placed over the microbeam port to irradiate cells along a 35 μm wide, 6 mm long path. Immunohistochemical analysis of γ-H2AX foci formation in tissue sections revealed, compared to control tissue, proton-induced γ-H2AX foci formation in one of the two epidermal layers of the mouse ear. Strikingly, a higher number of cells than expected showed foci from direct irradiation effects. Although the proton-irradiated line w... Keratinocytes - radiation effects Ear - radiation effects Gene Expression - radiation effects Histones - biosynthesis DNA Damage - radiation effects G. Randers-Pehrson oth L. B. Smilenov oth N. J. Kleiman oth E. Young oth B. Ponnayia oth D. J. Brenner oth Enthalten in Radiation research Lawrence, Kan. : Soc., 1954 184(2015), 2, Seite 219-225 (DE-627)129270644 (DE-600)80322-4 (DE-576)01446120X 0033-7587 nnns volume:184 year:2015 number:2 pages:219-225 http://dx.doi.org/10.1667/RR14057.1 Volltext http://www.bioone.org/doi/full/10.1667/RR14057.1 http://www.ncbi.nlm.nih.gov/pubmed/26207682 http://search.proquest.com/docview/1707037125 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-PHA GBV_ILN_170 GBV_ILN_2006 GBV_ILN_4112 GBV_ILN_4219 GBV_ILN_4256 GBV_ILN_4305 GBV_ILN_4306 WA 15000 44.64 AVZ 44.40 AVZ 33.30 AVZ AR 184 2015 2 219-225
language	English
source	Enthalten in Radiation research 184(2015), 2, Seite 219-225 volume:184 year:2015 number:2 pages:219-225
sourceStr	Enthalten in Radiation research 184(2015), 2, Seite 219-225 volume:184 year:2015 number:2 pages:219-225
format_phy_str_mv	Article
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topic_facet	Keratinocytes - radiation effects Ear - radiation effects Gene Expression - radiation effects Histones - biosynthesis DNA Damage - radiation effects
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container_title	Radiation research
authorswithroles_txt_mv	M. Buonanno @@aut@@ G. Randers-Pehrson @@oth@@ L. B. Smilenov @@oth@@ N. J. Kleiman @@oth@@ E. Young @@oth@@ B. Ponnayia @@oth@@ D. J. Brenner @@oth@@
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author	M. Buonanno
spellingShingle	M. Buonanno ddc 610 rvk WA 15000 bkl 44.64 bkl 44.40 bkl 33.30 misc Keratinocytes - radiation effects misc Ear - radiation effects misc Gene Expression - radiation effects misc Histones - biosynthesis misc DNA Damage - radiation effects A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation
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topic_title	610 530 DNB WA 15000 AVZ rvk 44.64 bkl 44.40 bkl 33.30 bkl A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation Keratinocytes - radiation effects Ear - radiation effects Gene Expression - radiation effects Histones - biosynthesis DNA Damage - radiation effects
topic	ddc 610 rvk WA 15000 bkl 44.64 bkl 44.40 bkl 33.30 misc Keratinocytes - radiation effects misc Ear - radiation effects misc Gene Expression - radiation effects misc Histones - biosynthesis misc DNA Damage - radiation effects
topic_unstemmed	ddc 610 rvk WA 15000 bkl 44.64 bkl 44.40 bkl 33.30 misc Keratinocytes - radiation effects misc Ear - radiation effects misc Gene Expression - radiation effects misc Histones - biosynthesis misc DNA Damage - radiation effects
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title	A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation
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title_full	A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation
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title_sort	mouse ear model for bystander studies induced by microbeam irradiation
title_auth	A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation
abstract	Radiation-induced bystander effects have been observed in vitro and in cell and tissue culture models, however, there are few reported studies showing these effects in vivo. To our knowledge, this is the first reported study on bystander effects induced by microbeam irradiation in an intact living mammal. The mouse ear was used to investigate radiation-induced bystander effects in keratinocytes, utilizing a 3 MeV proton microbeam (LET 13.1 keV/μm) with a range in skin of about 135 μm. Using a custom-designed holder, the ear of an anesthetized C57BL/6J mouse was flattened by gentle suction and placed over the microbeam port to irradiate cells along a 35 μm wide, 6 mm long path. Immunohistochemical analysis of γ-H2AX foci formation in tissue sections revealed, compared to control tissue, proton-induced γ-H2AX foci formation in one of the two epidermal layers of the mouse ear. Strikingly, a higher number of cells than expected showed foci from direct irradiation effects. Although the proton-irradiated line w...
abstractGer	Radiation-induced bystander effects have been observed in vitro and in cell and tissue culture models, however, there are few reported studies showing these effects in vivo. To our knowledge, this is the first reported study on bystander effects induced by microbeam irradiation in an intact living mammal. The mouse ear was used to investigate radiation-induced bystander effects in keratinocytes, utilizing a 3 MeV proton microbeam (LET 13.1 keV/μm) with a range in skin of about 135 μm. Using a custom-designed holder, the ear of an anesthetized C57BL/6J mouse was flattened by gentle suction and placed over the microbeam port to irradiate cells along a 35 μm wide, 6 mm long path. Immunohistochemical analysis of γ-H2AX foci formation in tissue sections revealed, compared to control tissue, proton-induced γ-H2AX foci formation in one of the two epidermal layers of the mouse ear. Strikingly, a higher number of cells than expected showed foci from direct irradiation effects. Although the proton-irradiated line w...
abstract_unstemmed	Radiation-induced bystander effects have been observed in vitro and in cell and tissue culture models, however, there are few reported studies showing these effects in vivo. To our knowledge, this is the first reported study on bystander effects induced by microbeam irradiation in an intact living mammal. The mouse ear was used to investigate radiation-induced bystander effects in keratinocytes, utilizing a 3 MeV proton microbeam (LET 13.1 keV/μm) with a range in skin of about 135 μm. Using a custom-designed holder, the ear of an anesthetized C57BL/6J mouse was flattened by gentle suction and placed over the microbeam port to irradiate cells along a 35 μm wide, 6 mm long path. Immunohistochemical analysis of γ-H2AX foci formation in tissue sections revealed, compared to control tissue, proton-induced γ-H2AX foci formation in one of the two epidermal layers of the mouse ear. Strikingly, a higher number of cells than expected showed foci from direct irradiation effects. Although the proton-irradiated line w...
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container_issue	2
title_short	A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation
url	http://dx.doi.org/10.1667/RR14057.1 http://www.bioone.org/doi/full/10.1667/RR14057.1 http://www.ncbi.nlm.nih.gov/pubmed/26207682 http://search.proquest.com/docview/1707037125
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author2	G. Randers-Pehrson L. B. Smilenov N. J. Kleiman E. Young B. Ponnayia D. J. Brenner
author2Str	G. Randers-Pehrson L. B. Smilenov N. J. Kleiman E. Young B. Ponnayia D. J. Brenner
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up_date	2024-07-04T04:58:53.433Z
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