Struvite Precipitation Induced by a Novel Sulfate-Reducing Bacterium Acinetobacter calcoaceticus SRB4 Isolated from River Sediment
This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were...
Ausführliche Beschreibung
Autor*in: |
Han, Zuozhen [verfasserIn] |
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Format: |
Artikel |
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Sprache: |
Englisch |
Erschienen: |
2015 |
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Rechteinformationen: |
Nutzungsrecht: Copyright © Taylor & Francis Group, LLC 2015 |
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Übergeordnetes Werk: |
Enthalten in: Geomicrobiology journal - New York, NY : Crane, Russak & Co., 1978, 32(2015), 10, Seite 868 |
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Übergeordnetes Werk: |
volume:32 ; year:2015 ; number:10 ; pages:868 |
Links: |
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DOI / URN: |
10.1080/01490451.2015.1016247 |
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Katalog-ID: |
OLC1963415051 |
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520 | |a This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. | ||
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700 | 1 | |a Wang, Jie |4 oth | |
700 | 1 | |a Li, Hao |4 oth | |
700 | 1 | |a Wang, Yuqin |4 oth | |
700 | 1 | |a Du, Han |4 oth | |
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10.1080/01490451.2015.1016247 doi PQ20160617 (DE-627)OLC1963415051 (DE-599)GBVOLC1963415051 (PRQ)i1452-1dfad5839467e5b70b2518de74a7a1a52916ddc9e96f40a2b7c4d8a9c265cf500 (KEY)0016606520150000032001000868struviteprecipitationinducedbyanovelsulfatereducin DE-627 ger DE-627 rakwb eng 570 ZDB 38.32 bkl 38.30 bkl 42.30 bkl 42.44 bkl Han, Zuozhen verfasserin aut Struvite Precipitation Induced by a Novel Sulfate-Reducing Bacterium Acinetobacter calcoaceticus SRB4 Isolated from River Sediment 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. Nutzungsrecht: Copyright © Taylor & Francis Group, LLC 2015 biomineralization preferential orientation sulfate-reducing bacteria phosphorus recycling Acinetobacter calcoaceticus SRB4 struvite Zhao, Yanyang oth Yan, Huaxiao oth Zhao, Hui oth Han, Mei oth Sun, Bin oth Sun, Xiaoying oth Hou, Feifei oth Sun, Hui oth Han, Li oth Sun, Yanbiao oth Wang, Jie oth Li, Hao oth Wang, Yuqin oth Du, Han oth Enthalten in Geomicrobiology journal New York, NY : Crane, Russak & Co., 1978 32(2015), 10, Seite 868 (DE-627)130049409 (DE-600)433755-4 (DE-576)015587754 0149-0451 nnns volume:32 year:2015 number:10 pages:868 http://dx.doi.org/10.1080/01490451.2015.1016247 Volltext http://www.tandfonline.com/doi/abs/10.1080/01490451.2015.1016247 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-GGO GBV_ILN_21 GBV_ILN_120 GBV_ILN_2027 38.32 AVZ 38.30 AVZ 42.30 AVZ 42.44 AVZ AR 32 2015 10 868 |
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10.1080/01490451.2015.1016247 doi PQ20160617 (DE-627)OLC1963415051 (DE-599)GBVOLC1963415051 (PRQ)i1452-1dfad5839467e5b70b2518de74a7a1a52916ddc9e96f40a2b7c4d8a9c265cf500 (KEY)0016606520150000032001000868struviteprecipitationinducedbyanovelsulfatereducin DE-627 ger DE-627 rakwb eng 570 ZDB 38.32 bkl 38.30 bkl 42.30 bkl 42.44 bkl Han, Zuozhen verfasserin aut Struvite Precipitation Induced by a Novel Sulfate-Reducing Bacterium Acinetobacter calcoaceticus SRB4 Isolated from River Sediment 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. Nutzungsrecht: Copyright © Taylor & Francis Group, LLC 2015 biomineralization preferential orientation sulfate-reducing bacteria phosphorus recycling Acinetobacter calcoaceticus SRB4 struvite Zhao, Yanyang oth Yan, Huaxiao oth Zhao, Hui oth Han, Mei oth Sun, Bin oth Sun, Xiaoying oth Hou, Feifei oth Sun, Hui oth Han, Li oth Sun, Yanbiao oth Wang, Jie oth Li, Hao oth Wang, Yuqin oth Du, Han oth Enthalten in Geomicrobiology journal New York, NY : Crane, Russak & Co., 1978 32(2015), 10, Seite 868 (DE-627)130049409 (DE-600)433755-4 (DE-576)015587754 0149-0451 nnns volume:32 year:2015 number:10 pages:868 http://dx.doi.org/10.1080/01490451.2015.1016247 Volltext http://www.tandfonline.com/doi/abs/10.1080/01490451.2015.1016247 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-GGO GBV_ILN_21 GBV_ILN_120 GBV_ILN_2027 38.32 AVZ 38.30 AVZ 42.30 AVZ 42.44 AVZ AR 32 2015 10 868 |
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10.1080/01490451.2015.1016247 doi PQ20160617 (DE-627)OLC1963415051 (DE-599)GBVOLC1963415051 (PRQ)i1452-1dfad5839467e5b70b2518de74a7a1a52916ddc9e96f40a2b7c4d8a9c265cf500 (KEY)0016606520150000032001000868struviteprecipitationinducedbyanovelsulfatereducin DE-627 ger DE-627 rakwb eng 570 ZDB 38.32 bkl 38.30 bkl 42.30 bkl 42.44 bkl Han, Zuozhen verfasserin aut Struvite Precipitation Induced by a Novel Sulfate-Reducing Bacterium Acinetobacter calcoaceticus SRB4 Isolated from River Sediment 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. Nutzungsrecht: Copyright © Taylor & Francis Group, LLC 2015 biomineralization preferential orientation sulfate-reducing bacteria phosphorus recycling Acinetobacter calcoaceticus SRB4 struvite Zhao, Yanyang oth Yan, Huaxiao oth Zhao, Hui oth Han, Mei oth Sun, Bin oth Sun, Xiaoying oth Hou, Feifei oth Sun, Hui oth Han, Li oth Sun, Yanbiao oth Wang, Jie oth Li, Hao oth Wang, Yuqin oth Du, Han oth Enthalten in Geomicrobiology journal New York, NY : Crane, Russak & Co., 1978 32(2015), 10, Seite 868 (DE-627)130049409 (DE-600)433755-4 (DE-576)015587754 0149-0451 nnns volume:32 year:2015 number:10 pages:868 http://dx.doi.org/10.1080/01490451.2015.1016247 Volltext http://www.tandfonline.com/doi/abs/10.1080/01490451.2015.1016247 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-GGO GBV_ILN_21 GBV_ILN_120 GBV_ILN_2027 38.32 AVZ 38.30 AVZ 42.30 AVZ 42.44 AVZ AR 32 2015 10 868 |
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10.1080/01490451.2015.1016247 doi PQ20160617 (DE-627)OLC1963415051 (DE-599)GBVOLC1963415051 (PRQ)i1452-1dfad5839467e5b70b2518de74a7a1a52916ddc9e96f40a2b7c4d8a9c265cf500 (KEY)0016606520150000032001000868struviteprecipitationinducedbyanovelsulfatereducin DE-627 ger DE-627 rakwb eng 570 ZDB 38.32 bkl 38.30 bkl 42.30 bkl 42.44 bkl Han, Zuozhen verfasserin aut Struvite Precipitation Induced by a Novel Sulfate-Reducing Bacterium Acinetobacter calcoaceticus SRB4 Isolated from River Sediment 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. Nutzungsrecht: Copyright © Taylor & Francis Group, LLC 2015 biomineralization preferential orientation sulfate-reducing bacteria phosphorus recycling Acinetobacter calcoaceticus SRB4 struvite Zhao, Yanyang oth Yan, Huaxiao oth Zhao, Hui oth Han, Mei oth Sun, Bin oth Sun, Xiaoying oth Hou, Feifei oth Sun, Hui oth Han, Li oth Sun, Yanbiao oth Wang, Jie oth Li, Hao oth Wang, Yuqin oth Du, Han oth Enthalten in Geomicrobiology journal New York, NY : Crane, Russak & Co., 1978 32(2015), 10, Seite 868 (DE-627)130049409 (DE-600)433755-4 (DE-576)015587754 0149-0451 nnns volume:32 year:2015 number:10 pages:868 http://dx.doi.org/10.1080/01490451.2015.1016247 Volltext http://www.tandfonline.com/doi/abs/10.1080/01490451.2015.1016247 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-GGO GBV_ILN_21 GBV_ILN_120 GBV_ILN_2027 38.32 AVZ 38.30 AVZ 42.30 AVZ 42.44 AVZ AR 32 2015 10 868 |
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10.1080/01490451.2015.1016247 doi PQ20160617 (DE-627)OLC1963415051 (DE-599)GBVOLC1963415051 (PRQ)i1452-1dfad5839467e5b70b2518de74a7a1a52916ddc9e96f40a2b7c4d8a9c265cf500 (KEY)0016606520150000032001000868struviteprecipitationinducedbyanovelsulfatereducin DE-627 ger DE-627 rakwb eng 570 ZDB 38.32 bkl 38.30 bkl 42.30 bkl 42.44 bkl Han, Zuozhen verfasserin aut Struvite Precipitation Induced by a Novel Sulfate-Reducing Bacterium Acinetobacter calcoaceticus SRB4 Isolated from River Sediment 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. Nutzungsrecht: Copyright © Taylor & Francis Group, LLC 2015 biomineralization preferential orientation sulfate-reducing bacteria phosphorus recycling Acinetobacter calcoaceticus SRB4 struvite Zhao, Yanyang oth Yan, Huaxiao oth Zhao, Hui oth Han, Mei oth Sun, Bin oth Sun, Xiaoying oth Hou, Feifei oth Sun, Hui oth Han, Li oth Sun, Yanbiao oth Wang, Jie oth Li, Hao oth Wang, Yuqin oth Du, Han oth Enthalten in Geomicrobiology journal New York, NY : Crane, Russak & Co., 1978 32(2015), 10, Seite 868 (DE-627)130049409 (DE-600)433755-4 (DE-576)015587754 0149-0451 nnns volume:32 year:2015 number:10 pages:868 http://dx.doi.org/10.1080/01490451.2015.1016247 Volltext http://www.tandfonline.com/doi/abs/10.1080/01490451.2015.1016247 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-GGO GBV_ILN_21 GBV_ILN_120 GBV_ILN_2027 38.32 AVZ 38.30 AVZ 42.30 AVZ 42.44 AVZ AR 32 2015 10 868 |
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struvite precipitation induced by a novel sulfate-reducing bacterium acinetobacter calcoaceticus srb4 isolated from river sediment |
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Struvite Precipitation Induced by a Novel Sulfate-Reducing Bacterium Acinetobacter calcoaceticus SRB4 Isolated from River Sediment |
abstract |
This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. |
abstractGer |
This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. |
abstract_unstemmed |
This article presents a study of struvite formation in liquid medium induced by the sulfate-reducing bacterium Acinetobacter calcoaceticus SRB4, a strain isolated from river sediment. We identified the bacterial strain A. calcoaceticus SRB4 and analyzed its micromorphology. The minerals formed were studied with an electroprobe microanalyzer, Fourier transform infrared spectroscopy, high-resolution transmission electron microscopy, selected-area electron diffraction, X-ray diffraction, thermogravimetry, differential thermogravimetry, and differential scanning calorimetry. Acinetobacter calcoaceticus SRB4 was found to induce struvite precipitation, whereas sterile control cultures did not. Many transparent stick-shaped struvite precipitates were distributed at the bottom of the conical flasks in the experimental group. Most bacteria were spherical and a large quantity of spherical struvite particles (less than 200 nm in diameter) adhered to the bacterial surface. An electron probe microanalysis showed that the precipitates contained C, O, P, Mg, and other elements. Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. Our study extends our understanding of the phosphate biomineralization mechanism and should prove useful in recycling phosphorus in wastewater. |
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Struvite Precipitation Induced by a Novel Sulfate-Reducing Bacterium Acinetobacter calcoaceticus SRB4 Isolated from River Sediment |
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Zhao, Yanyang Yan, Huaxiao Zhao, Hui Han, Mei Sun, Bin Sun, Xiaoying Hou, Feifei Sun, Hui Han, Li Sun, Yanbiao Wang, Jie Li, Hao Wang, Yuqin Du, Han |
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Fourier transformation infrared spectra showed that the precipitates contained crystalline water, NH 4 + , and PO 4 3− groups. X-ray diffraction spectra showed that the precipitates were struvite crystals, with preferential orientation and lattice distortion. Thermogravimetry showed that the weight loss was caused by the evaporation of crystalline water at temperatures lower than 136°C and the release of ammonia from struvite at temperatures of 136-228.5°C. In this article, we discuss the possible mechanism of struvite formation and the possible role played by A. calcoaceticus SRB4. 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