Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds

The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in...
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Autor*in:	Singh, Shweta [verfasserIn] Rai, Pankaj Kumar Chau, Rocky Ravi, Alok Kumar Neilan, Brett A Asthana, Ravi Kumar

Format:	Artikel
Sprache:	Englisch

Erschienen:	2015

Rechteinformationen:	Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved.

Schlagwörter:	Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism

Übergeordnetes Werk:	Enthalten in: Water research - Amsterdam [u.a.] : Elsevier, Pergamon, 1967, 69(2015), Seite 131-142
Übergeordnetes Werk:	volume:69 ; year:2015 ; pages:131-142

Links:	Volltext Link aufrufen

DOI / URN:	10.1016/j.watres.2014.11.015

Katalog-ID:	OLC1963549759

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520			\|a The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production.
540			\|a Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved.
650		4	\|a Ponds - microbiology
650		4	\|a Microcystins - biosynthesis
650		4	\|a RNA, Ribosomal, 16S - genetics
650		4	\|a Microcystis - metabolism
700	1		\|a Rai, Pankaj Kumar \|4 oth
700	1		\|a Chau, Rocky \|4 oth
700	1		\|a Ravi, Alok Kumar \|4 oth
700	1		\|a Neilan, Brett A \|4 oth
700	1		\|a Asthana, Ravi Kumar \|4 oth
773	0	8	\|i Enthalten in \|t Water research \|d Amsterdam [u.a.] : Elsevier, Pergamon, 1967 \|g 69(2015), Seite 131-142 \|w (DE-627)129471860 \|w (DE-600)202613-2 \|w (DE-576)014841630 \|x 0043-1354 \|7 nnns
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allfields	10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142
spelling	10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142
allfields_unstemmed	10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142
allfieldsGer	10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142
allfieldsSound	10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142
language	English
source	Enthalten in Water research 69(2015), Seite 131-142 volume:69 year:2015 pages:131-142
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topic_facet	Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism
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container_title	Water research
authorswithroles_txt_mv	Singh, Shweta @@aut@@ Rai, Pankaj Kumar @@oth@@ Chau, Rocky @@oth@@ Ravi, Alok Kumar @@oth@@ Neilan, Brett A @@oth@@ Asthana, Ravi Kumar @@oth@@
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author	Singh, Shweta
spellingShingle	Singh, Shweta ddc 550 misc Ponds - microbiology misc Microcystins - biosynthesis misc RNA, Ribosomal, 16S - genetics misc Microcystis - metabolism Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds
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topic_title	550 DNB Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism
topic	ddc 550 misc Ponds - microbiology misc Microcystins - biosynthesis misc RNA, Ribosomal, 16S - genetics misc Microcystis - metabolism
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title	Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds
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title_full	Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds
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title_sort	temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds
title_auth	Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds
abstract	The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production.
abstractGer	The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production.
abstract_unstemmed	The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production.
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title_short	Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds
url	http://dx.doi.org/10.1016/j.watres.2014.11.015 http://www.ncbi.nlm.nih.gov/pubmed/25463934
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up_date	2024-07-04T05:59:39.180Z
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