Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds
The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in...
Ausführliche Beschreibung
Autor*in: |
Singh, Shweta [verfasserIn] |
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Format: |
Artikel |
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Sprache: |
Englisch |
Erschienen: |
2015 |
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Rechteinformationen: |
Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. |
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Schlagwörter: |
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Übergeordnetes Werk: |
Enthalten in: Water research - Amsterdam [u.a.] : Elsevier, Pergamon, 1967, 69(2015), Seite 131-142 |
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Übergeordnetes Werk: |
volume:69 ; year:2015 ; pages:131-142 |
Links: |
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DOI / URN: |
10.1016/j.watres.2014.11.015 |
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OLC1963549759 |
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520 | |a The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. | ||
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650 | 4 | |a Microcystins - biosynthesis | |
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700 | 1 | |a Neilan, Brett A |4 oth | |
700 | 1 | |a Asthana, Ravi Kumar |4 oth | |
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10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142 |
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10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142 |
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10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142 |
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10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142 |
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10.1016/j.watres.2014.11.015 doi PQ20160617 (DE-627)OLC1963549759 (DE-599)GBVOLC1963549759 (PRQ)c1864-5041039690144b5cd9c42ce1e115cf8a3adf478185e895bf2bd9673009bda9360 (KEY)0018203620150000069000000131temporalvariationsinmicrocystinproducingcellsandmi DE-627 ger DE-627 rakwb eng 550 DNB Singh, Shweta verfasserin aut Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. Nutzungsrecht: Copyright © 2014 Elsevier Ltd. All rights reserved. Ponds - microbiology Microcystins - biosynthesis RNA, Ribosomal, 16S - genetics Microcystis - metabolism Rai, Pankaj Kumar oth Chau, Rocky oth Ravi, Alok Kumar oth Neilan, Brett A oth Asthana, Ravi Kumar oth Enthalten in Water research Amsterdam [u.a.] : Elsevier, Pergamon, 1967 69(2015), Seite 131-142 (DE-627)129471860 (DE-600)202613-2 (DE-576)014841630 0043-1354 nnns volume:69 year:2015 pages:131-142 http://dx.doi.org/10.1016/j.watres.2014.11.015 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25463934 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OPC-GGO GBV_ILN_20 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4112 GBV_ILN_4219 AR 69 2015 131-142 |
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Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds |
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Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds |
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temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds |
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Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds |
abstract |
The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. |
abstractGer |
The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. |
abstract_unstemmed |
The relationship between microcystin production, microcystin-producing cyanobacteria, including Microcystis spp., and various biological and physicochemical parameters in Sankuldhara and Lakshmikund, situated in the same geographical area was studied over a period of 1.5 years. Seasonal variation in cyanobacterial 16S rRNA, Microcystis spp. 16S rRNA, mcyA and mcyB genes were quantitatively determined by real-time PCR. Microcystis was the dominant microcystin producer in both study sites constituting 67% and 97% of the total microcystin-producing cyanobacteria at Sankuldhara and Lakshmikund, respectively. Microcystin concentrations were 2.19-39.60 μg/L and 15.22-128.14 μg/L at Sankuldhara and Lakshmikund, respectively, as determined by LC-MS. Principal component analysis revealed a strong positive correlation between microcystin concentration and the copy number of mcyA and mcyB, chlorophyll a and cyanobacterial biomass at both sites. The higher microcystin concentrations in Lakshmikund pond were attributed to the high copy number of mcy genes present coupled with the pond's eutrophication status, as indicated by high total algal biomass, high chlorophyll a content, high nutrient load and low DO. Therefore, a significant difference in microcystin concentrations, correlating with these various biological and physicochemical parameters, confirms the importance of local environmental variables in the overall regulation of microcystins production. |
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title_short |
Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds |
url |
http://dx.doi.org/10.1016/j.watres.2014.11.015 http://www.ncbi.nlm.nih.gov/pubmed/25463934 |
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Rai, Pankaj Kumar Chau, Rocky Ravi, Alok Kumar Neilan, Brett A Asthana, Ravi Kumar |
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