Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.)

In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Nair, Prakash M Gopalakrishnan [verfasserIn] Chung, Ill Min

Format:	Artikel
Sprache:	Englisch

Erschienen:	2015

Rechteinformationen:	Nutzungsrecht: Copyright © 2014 Elsevier Inc. All rights reserved.

Schlagwörter:	Peroxidase - metabolism Peroxidases - metabolism Plant Roots - drug effects Superoxide Dismutase - metabolism Nanoparticles - toxicity Lipid Peroxidation - drug effects Hydrogen Peroxide - metabolism Copper - toxicity Seedling - metabolism Seedling - drug effects Mustard Plant - growth & development Catalase - metabolism Chlorophyll - metabolism Lignin - metabolism Ascorbate Peroxidases - metabolism Oxidative Stress - drug effects Mustard Plant - drug effects Mustard Plant - enzymology

Übergeordnetes Werk:	Enthalten in: Ecotoxicology and environmental safety - Amsterdam : Elsevier, 1977, 113(2015), Seite 302-313
Übergeordnetes Werk:	volume:113 ; year:2015 ; pages:302-313

Links:	Volltext Link aufrufen

DOI / URN:	10.1016/j.ecoenv.2014.12.013

Katalog-ID:	OLC1964314380

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520			\|a In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs.
540			\|a Nutzungsrecht: Copyright © 2014 Elsevier Inc. All rights reserved.
650		4	\|a Peroxidase - metabolism
650		4	\|a Peroxidases - metabolism
650		4	\|a Plant Roots - drug effects
650		4	\|a Superoxide Dismutase - metabolism
650		4	\|a Nanoparticles - toxicity
650		4	\|a Lipid Peroxidation - drug effects
650		4	\|a Hydrogen Peroxide - metabolism
650		4	\|a Copper - toxicity
650		4	\|a Seedling - metabolism
650		4	\|a Seedling - drug effects
650		4	\|a Mustard Plant - growth & development
650		4	\|a Catalase - metabolism
650		4	\|a Chlorophyll - metabolism
650		4	\|a Lignin - metabolism
650		4	\|a Ascorbate Peroxidases - metabolism
650		4	\|a Oxidative Stress - drug effects
650		4	\|a Mustard Plant - drug effects
650		4	\|a Mustard Plant - enzymology
700	1		\|a Chung, Ill Min \|4 oth
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allfields	10.1016/j.ecoenv.2014.12.013 doi PQ20160617 (DE-627)OLC1964314380 (DE-599)GBVOLC1964314380 (PRQ)c2158-4351343e0d9bb25ae606849eaa13069df99c9b9ba6d7f41c5b9110a720d786f50 (KEY)0029001720150000113000000302studyonthecorrelationbetweencopperoxidenanoparticl DE-627 ger DE-627 rakwb eng 610 570 DNB 44.13 bkl 44.00 bkl Nair, Prakash M Gopalakrishnan verfasserin aut Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.) 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs. Nutzungsrecht: Copyright © 2014 Elsevier Inc. All rights reserved. Peroxidase - metabolism Peroxidases - metabolism Plant Roots - drug effects Superoxide Dismutase - metabolism Nanoparticles - toxicity Lipid Peroxidation - drug effects Hydrogen Peroxide - metabolism Copper - toxicity Seedling - metabolism Seedling - drug effects Mustard Plant - growth & development Catalase - metabolism Chlorophyll - metabolism Lignin - metabolism Ascorbate Peroxidases - metabolism Oxidative Stress - drug effects Mustard Plant - drug effects Mustard Plant - enzymology Chung, Ill Min oth Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 113(2015), Seite 302-313 (DE-627)130055611 (DE-600)436536-7 (DE-576)015593118 0147-6513 nnns volume:113 year:2015 pages:302-313 http://dx.doi.org/10.1016/j.ecoenv.2014.12.013 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25528486 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4219 44.13 AVZ 44.00 AVZ AR 113 2015 302-313
spelling	10.1016/j.ecoenv.2014.12.013 doi PQ20160617 (DE-627)OLC1964314380 (DE-599)GBVOLC1964314380 (PRQ)c2158-4351343e0d9bb25ae606849eaa13069df99c9b9ba6d7f41c5b9110a720d786f50 (KEY)0029001720150000113000000302studyonthecorrelationbetweencopperoxidenanoparticl DE-627 ger DE-627 rakwb eng 610 570 DNB 44.13 bkl 44.00 bkl Nair, Prakash M Gopalakrishnan verfasserin aut Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.) 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs. Nutzungsrecht: Copyright © 2014 Elsevier Inc. All rights reserved. Peroxidase - metabolism Peroxidases - metabolism Plant Roots - drug effects Superoxide Dismutase - metabolism Nanoparticles - toxicity Lipid Peroxidation - drug effects Hydrogen Peroxide - metabolism Copper - toxicity Seedling - metabolism Seedling - drug effects Mustard Plant - growth & development Catalase - metabolism Chlorophyll - metabolism Lignin - metabolism Ascorbate Peroxidases - metabolism Oxidative Stress - drug effects Mustard Plant - drug effects Mustard Plant - enzymology Chung, Ill Min oth Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 113(2015), Seite 302-313 (DE-627)130055611 (DE-600)436536-7 (DE-576)015593118 0147-6513 nnns volume:113 year:2015 pages:302-313 http://dx.doi.org/10.1016/j.ecoenv.2014.12.013 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25528486 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4219 44.13 AVZ 44.00 AVZ AR 113 2015 302-313
allfields_unstemmed	10.1016/j.ecoenv.2014.12.013 doi PQ20160617 (DE-627)OLC1964314380 (DE-599)GBVOLC1964314380 (PRQ)c2158-4351343e0d9bb25ae606849eaa13069df99c9b9ba6d7f41c5b9110a720d786f50 (KEY)0029001720150000113000000302studyonthecorrelationbetweencopperoxidenanoparticl DE-627 ger DE-627 rakwb eng 610 570 DNB 44.13 bkl 44.00 bkl Nair, Prakash M Gopalakrishnan verfasserin aut Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.) 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs. Nutzungsrecht: Copyright © 2014 Elsevier Inc. All rights reserved. Peroxidase - metabolism Peroxidases - metabolism Plant Roots - drug effects Superoxide Dismutase - metabolism Nanoparticles - toxicity Lipid Peroxidation - drug effects Hydrogen Peroxide - metabolism Copper - toxicity Seedling - metabolism Seedling - drug effects Mustard Plant - growth & development Catalase - metabolism Chlorophyll - metabolism Lignin - metabolism Ascorbate Peroxidases - metabolism Oxidative Stress - drug effects Mustard Plant - drug effects Mustard Plant - enzymology Chung, Ill Min oth Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 113(2015), Seite 302-313 (DE-627)130055611 (DE-600)436536-7 (DE-576)015593118 0147-6513 nnns volume:113 year:2015 pages:302-313 http://dx.doi.org/10.1016/j.ecoenv.2014.12.013 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25528486 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4219 44.13 AVZ 44.00 AVZ AR 113 2015 302-313
allfieldsGer	10.1016/j.ecoenv.2014.12.013 doi PQ20160617 (DE-627)OLC1964314380 (DE-599)GBVOLC1964314380 (PRQ)c2158-4351343e0d9bb25ae606849eaa13069df99c9b9ba6d7f41c5b9110a720d786f50 (KEY)0029001720150000113000000302studyonthecorrelationbetweencopperoxidenanoparticl DE-627 ger DE-627 rakwb eng 610 570 DNB 44.13 bkl 44.00 bkl Nair, Prakash M Gopalakrishnan verfasserin aut Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.) 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs. Nutzungsrecht: Copyright © 2014 Elsevier Inc. All rights reserved. Peroxidase - metabolism Peroxidases - metabolism Plant Roots - drug effects Superoxide Dismutase - metabolism Nanoparticles - toxicity Lipid Peroxidation - drug effects Hydrogen Peroxide - metabolism Copper - toxicity Seedling - metabolism Seedling - drug effects Mustard Plant - growth & development Catalase - metabolism Chlorophyll - metabolism Lignin - metabolism Ascorbate Peroxidases - metabolism Oxidative Stress - drug effects Mustard Plant - drug effects Mustard Plant - enzymology Chung, Ill Min oth Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 113(2015), Seite 302-313 (DE-627)130055611 (DE-600)436536-7 (DE-576)015593118 0147-6513 nnns volume:113 year:2015 pages:302-313 http://dx.doi.org/10.1016/j.ecoenv.2014.12.013 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25528486 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4219 44.13 AVZ 44.00 AVZ AR 113 2015 302-313
allfieldsSound	10.1016/j.ecoenv.2014.12.013 doi PQ20160617 (DE-627)OLC1964314380 (DE-599)GBVOLC1964314380 (PRQ)c2158-4351343e0d9bb25ae606849eaa13069df99c9b9ba6d7f41c5b9110a720d786f50 (KEY)0029001720150000113000000302studyonthecorrelationbetweencopperoxidenanoparticl DE-627 ger DE-627 rakwb eng 610 570 DNB 44.13 bkl 44.00 bkl Nair, Prakash M Gopalakrishnan verfasserin aut Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.) 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs. Nutzungsrecht: Copyright © 2014 Elsevier Inc. All rights reserved. Peroxidase - metabolism Peroxidases - metabolism Plant Roots - drug effects Superoxide Dismutase - metabolism Nanoparticles - toxicity Lipid Peroxidation - drug effects Hydrogen Peroxide - metabolism Copper - toxicity Seedling - metabolism Seedling - drug effects Mustard Plant - growth & development Catalase - metabolism Chlorophyll - metabolism Lignin - metabolism Ascorbate Peroxidases - metabolism Oxidative Stress - drug effects Mustard Plant - drug effects Mustard Plant - enzymology Chung, Ill Min oth Enthalten in Ecotoxicology and environmental safety Amsterdam : Elsevier, 1977 113(2015), Seite 302-313 (DE-627)130055611 (DE-600)436536-7 (DE-576)015593118 0147-6513 nnns volume:113 year:2015 pages:302-313 http://dx.doi.org/10.1016/j.ecoenv.2014.12.013 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25528486 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_4219 44.13 AVZ 44.00 AVZ AR 113 2015 302-313
language	English
source	Enthalten in Ecotoxicology and environmental safety 113(2015), Seite 302-313 volume:113 year:2015 pages:302-313
sourceStr	Enthalten in Ecotoxicology and environmental safety 113(2015), Seite 302-313 volume:113 year:2015 pages:302-313
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topic_facet	Peroxidase - metabolism Peroxidases - metabolism Plant Roots - drug effects Superoxide Dismutase - metabolism Nanoparticles - toxicity Lipid Peroxidation - drug effects Hydrogen Peroxide - metabolism Copper - toxicity Seedling - metabolism Seedling - drug effects Mustard Plant - growth & development Catalase - metabolism Chlorophyll - metabolism Lignin - metabolism Ascorbate Peroxidases - metabolism Oxidative Stress - drug effects Mustard Plant - drug effects Mustard Plant - enzymology
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author	Nair, Prakash M Gopalakrishnan
spellingShingle	Nair, Prakash M Gopalakrishnan ddc 610 bkl 44.13 bkl 44.00 misc Peroxidase - metabolism misc Peroxidases - metabolism misc Plant Roots - drug effects misc Superoxide Dismutase - metabolism misc Nanoparticles - toxicity misc Lipid Peroxidation - drug effects misc Hydrogen Peroxide - metabolism misc Copper - toxicity misc Seedling - metabolism misc Seedling - drug effects misc Mustard Plant - growth & development misc Catalase - metabolism misc Chlorophyll - metabolism misc Lignin - metabolism misc Ascorbate Peroxidases - metabolism misc Oxidative Stress - drug effects misc Mustard Plant - drug effects misc Mustard Plant - enzymology Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.)
authorStr	Nair, Prakash M Gopalakrishnan
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topic_title	610 570 DNB 44.13 bkl 44.00 bkl Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.) Peroxidase - metabolism Peroxidases - metabolism Plant Roots - drug effects Superoxide Dismutase - metabolism Nanoparticles - toxicity Lipid Peroxidation - drug effects Hydrogen Peroxide - metabolism Copper - toxicity Seedling - metabolism Seedling - drug effects Mustard Plant - growth & development Catalase - metabolism Chlorophyll - metabolism Lignin - metabolism Ascorbate Peroxidases - metabolism Oxidative Stress - drug effects Mustard Plant - drug effects Mustard Plant - enzymology
topic	ddc 610 bkl 44.13 bkl 44.00 misc Peroxidase - metabolism misc Peroxidases - metabolism misc Plant Roots - drug effects misc Superoxide Dismutase - metabolism misc Nanoparticles - toxicity misc Lipid Peroxidation - drug effects misc Hydrogen Peroxide - metabolism misc Copper - toxicity misc Seedling - metabolism misc Seedling - drug effects misc Mustard Plant - growth & development misc Catalase - metabolism misc Chlorophyll - metabolism misc Lignin - metabolism misc Ascorbate Peroxidases - metabolism misc Oxidative Stress - drug effects misc Mustard Plant - drug effects misc Mustard Plant - enzymology
topic_unstemmed	ddc 610 bkl 44.13 bkl 44.00 misc Peroxidase - metabolism misc Peroxidases - metabolism misc Plant Roots - drug effects misc Superoxide Dismutase - metabolism misc Nanoparticles - toxicity misc Lipid Peroxidation - drug effects misc Hydrogen Peroxide - metabolism misc Copper - toxicity misc Seedling - metabolism misc Seedling - drug effects misc Mustard Plant - growth & development misc Catalase - metabolism misc Chlorophyll - metabolism misc Lignin - metabolism misc Ascorbate Peroxidases - metabolism misc Oxidative Stress - drug effects misc Mustard Plant - drug effects misc Mustard Plant - enzymology
topic_browse	ddc 610 bkl 44.13 bkl 44.00 misc Peroxidase - metabolism misc Peroxidases - metabolism misc Plant Roots - drug effects misc Superoxide Dismutase - metabolism misc Nanoparticles - toxicity misc Lipid Peroxidation - drug effects misc Hydrogen Peroxide - metabolism misc Copper - toxicity misc Seedling - metabolism misc Seedling - drug effects misc Mustard Plant - growth & development misc Catalase - metabolism misc Chlorophyll - metabolism misc Lignin - metabolism misc Ascorbate Peroxidases - metabolism misc Oxidative Stress - drug effects misc Mustard Plant - drug effects misc Mustard Plant - enzymology
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title	Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.)
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title_full	Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.)
author_sort	Nair, Prakash M Gopalakrishnan
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author-letter	Nair, Prakash M Gopalakrishnan
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title_sort	study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in indian mustard (brassica juncea l.)
title_auth	Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.)
abstract	In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs.
abstractGer	In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs.
abstract_unstemmed	In this study, the morphological, physiological and molecular level effects of copper oxide nanoparticles (CuONPs) were studied in an economically important oil seed crop Brassica juncea L. The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs.
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title_short	Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.)
url	http://dx.doi.org/10.1016/j.ecoenv.2014.12.013 http://www.ncbi.nlm.nih.gov/pubmed/25528486
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The possible involvement of lignification on shoot-root growth retardation was also studied. The seedlings were exposed to 0, 20, 50, 100, 200, 400 and 500mg/L of CuONPs in semi-solid half strength Murashige and Skoog medium under controlled growth chamber conditions for 14 days. Exposure to CuONPs resulted in suppression of shoot-growth, reduction in total chlorophyll and carotenoids contents as well modification of root system architecture such as shortening of primary and lateral roots. Significant increases in hydrogen peroxide formation, peroxidase enzyme activity and lignification of shoots and roots were observed. The lipid peroxidation levels increased significantly in shoots and roots of B. juncea seedlings. Phloroglucinol-HCl staining revealed enhanced lignification of shoot and roots. Gene expression studies revealed significant activation of CuZn superoxide dismutase (CuZnSOD) in roots at all concentrations of CuONPs exposure. In shoots significant up-regulation of CuZnSOD gene was observed upon exposure to 100, 200 and 400 mg/L of CuONPs exposure. However no change in the expression levels of MnSOD gene was observed in both stem and roots. The expression of catalase (CAT) and ascorbate peroxidase (APX) genes were also not changed in shoots. However, significant inhibition of CAT and APX genes were observed in roots of B. juncea plants under exposure to 100, 200, 400 and 500 mg/L of CuONPs exposure. The SOD enzyme activity significantly increased in roots under exposure to 50-500 mg/L of CuONPs and in shoots as a result of exposure to 100-500 mg/L of CuONPs. The APX activity significantly decreased in roots upon exposure to 50-500 mg/L of CuONPs. In shoots, the APX activity significantly decreased upon exposure to 200-500 mg/L of CuONPs.</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Nutzungsrecht: Copyright © 2014 Elsevier Inc. All rights reserved.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Peroxidase - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Peroxidases - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Plant Roots - drug effects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Superoxide Dismutase - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Nanoparticles - toxicity</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lipid Peroxidation - drug effects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hydrogen Peroxide - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Copper - toxicity</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Seedling - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Seedling - drug effects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mustard Plant - growth & development</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Catalase - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chlorophyll - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Lignin - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Ascorbate Peroxidases - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Oxidative Stress - drug effects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mustard Plant - drug effects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mustard Plant - enzymology</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chung, Ill Min</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Ecotoxicology and environmental safety</subfield><subfield code="d">Amsterdam : Elsevier, 1977</subfield><subfield code="g">113(2015), Seite 302-313</subfield><subfield code="w">(DE-627)130055611</subfield><subfield code="w">(DE-600)436536-7</subfield><subfield code="w">(DE-576)015593118</subfield><subfield code="x">0147-6513</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:113</subfield><subfield code="g">year:2015</subfield><subfield code="g">pages:302-313</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://dx.doi.org/10.1016/j.ecoenv.2014.12.013</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://www.ncbi.nlm.nih.gov/pubmed/25528486</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_21</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4219</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.13</subfield><subfield code="q">AVZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.00</subfield><subfield code="q">AVZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">113</subfield><subfield code="j">2015</subfield><subfield code="h">302-313</subfield></datafield></record></collection>
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Study on the correlation between copper oxide nanoparticles induced growth suppression and enhanced lignification in Indian mustard (Brassica juncea L.)

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