Super-resolution microscopy based on fluorescence emission difference of cylindrical vector beams
We propose a novel fluorescence emission difference microscopy (FED) system based on focusing cylindrical vector beams. In conventional FED, a Gaussian beam and a 0-2[pi] vortex phase plate are used to generate solid and hollow spots. We focus radially polarized and azimuthally polarized cylindrical...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Rong, Zihao [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Rechteinformationen: |
Nutzungsrecht: © COPYRIGHT 2015 Elsevier B.V. |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Optics communications - Amsterdam [u.a.] : Elsevier, 1969, 354(2015), Seite 71-78 |
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| Übergeordnetes Werk: |
volume:354 ; year:2015 ; pages:71-78 |
| Links: |
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| DOI / URN: |
10.1016/j.optcom.2015.05.057 |
|---|
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| 520 | |a We propose a novel fluorescence emission difference microscopy (FED) system based on focusing cylindrical vector beams. In conventional FED, a Gaussian beam and a 0-2[pi] vortex phase plate are used to generate solid and hollow spots. We focus radially polarized and azimuthally polarized cylindrical vector beams to obtain an expanded solid spot and a shrunken hollow spot, taking advantage of the optical properties of cylindrical vector beams to improve the conventional FED performance. Our novel method enhances FED performance because the hollow spot size determines the FED resolution and an expanded solid spot effectively reduces negative side-lobe emergence during image processing. We demonstrate improved performance theoretically and experimentally using an in-house built FED. Our FED achieved resolution of less than [lambda]/4 in test images of 100nm nanoparticles, better than the confocal image resolution by a factor of approximately 1/3. We also discuss detailed simulation analyses and FED imaging of biological cells. | ||
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| 700 | 1 | |a Zhao, Guangyuan |4 oth | |
| 700 | 1 | |a Xu, Yingke |4 oth | |
| 700 | 1 | |a Liu, Xu |4 oth | |
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10.1016/j.optcom.2015.05.057 doi PQ20160617 (DE-627)OLC1964938767 (DE-599)GBVOLC1964938767 (PRQ)c1644-5193b93de8038309171914265a661b1fed9fe5cb0eeee89fefcb8c03a0c3f0470 (KEY)0038314720150000354000000071superresolutionmicroscopybasedonfluorescenceemissi DE-627 ger DE-627 rakwb eng 530 DNB Rong, Zihao verfasserin aut Super-resolution microscopy based on fluorescence emission difference of cylindrical vector beams 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier We propose a novel fluorescence emission difference microscopy (FED) system based on focusing cylindrical vector beams. In conventional FED, a Gaussian beam and a 0-2[pi] vortex phase plate are used to generate solid and hollow spots. We focus radially polarized and azimuthally polarized cylindrical vector beams to obtain an expanded solid spot and a shrunken hollow spot, taking advantage of the optical properties of cylindrical vector beams to improve the conventional FED performance. Our novel method enhances FED performance because the hollow spot size determines the FED resolution and an expanded solid spot effectively reduces negative side-lobe emergence during image processing. We demonstrate improved performance theoretically and experimentally using an in-house built FED. Our FED achieved resolution of less than [lambda]/4 in test images of 100nm nanoparticles, better than the confocal image resolution by a factor of approximately 1/3. We also discuss detailed simulation analyses and FED imaging of biological cells. Nutzungsrecht: © COPYRIGHT 2015 Elsevier B.V. Fluorescence Optical properties Biomedical engineering Fluorescence microscopy Kuang, Cuifang oth Fang, Yue oth Zhao, Guangyuan oth Xu, Yingke oth Liu, Xu oth Enthalten in Optics communications Amsterdam [u.a.] : Elsevier, 1969 354(2015), Seite 71-78 (DE-627)129361100 (DE-600)160795-9 (DE-576)014733552 0030-4018 nnns volume:354 year:2015 pages:71-78 http://dx.doi.org/10.1016/j.optcom.2015.05.057 Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY GBV_ILN_21 GBV_ILN_62 GBV_ILN_70 GBV_ILN_285 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_4036 AR 354 2015 71-78 |
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Super-resolution microscopy based on fluorescence emission difference of cylindrical vector beams |
| abstract |
We propose a novel fluorescence emission difference microscopy (FED) system based on focusing cylindrical vector beams. In conventional FED, a Gaussian beam and a 0-2[pi] vortex phase plate are used to generate solid and hollow spots. We focus radially polarized and azimuthally polarized cylindrical vector beams to obtain an expanded solid spot and a shrunken hollow spot, taking advantage of the optical properties of cylindrical vector beams to improve the conventional FED performance. Our novel method enhances FED performance because the hollow spot size determines the FED resolution and an expanded solid spot effectively reduces negative side-lobe emergence during image processing. We demonstrate improved performance theoretically and experimentally using an in-house built FED. Our FED achieved resolution of less than [lambda]/4 in test images of 100nm nanoparticles, better than the confocal image resolution by a factor of approximately 1/3. We also discuss detailed simulation analyses and FED imaging of biological cells. |
| abstractGer |
We propose a novel fluorescence emission difference microscopy (FED) system based on focusing cylindrical vector beams. In conventional FED, a Gaussian beam and a 0-2[pi] vortex phase plate are used to generate solid and hollow spots. We focus radially polarized and azimuthally polarized cylindrical vector beams to obtain an expanded solid spot and a shrunken hollow spot, taking advantage of the optical properties of cylindrical vector beams to improve the conventional FED performance. Our novel method enhances FED performance because the hollow spot size determines the FED resolution and an expanded solid spot effectively reduces negative side-lobe emergence during image processing. We demonstrate improved performance theoretically and experimentally using an in-house built FED. Our FED achieved resolution of less than [lambda]/4 in test images of 100nm nanoparticles, better than the confocal image resolution by a factor of approximately 1/3. We also discuss detailed simulation analyses and FED imaging of biological cells. |
| abstract_unstemmed |
We propose a novel fluorescence emission difference microscopy (FED) system based on focusing cylindrical vector beams. In conventional FED, a Gaussian beam and a 0-2[pi] vortex phase plate are used to generate solid and hollow spots. We focus radially polarized and azimuthally polarized cylindrical vector beams to obtain an expanded solid spot and a shrunken hollow spot, taking advantage of the optical properties of cylindrical vector beams to improve the conventional FED performance. Our novel method enhances FED performance because the hollow spot size determines the FED resolution and an expanded solid spot effectively reduces negative side-lobe emergence during image processing. We demonstrate improved performance theoretically and experimentally using an in-house built FED. Our FED achieved resolution of less than [lambda]/4 in test images of 100nm nanoparticles, better than the confocal image resolution by a factor of approximately 1/3. We also discuss detailed simulation analyses and FED imaging of biological cells. |
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| title_short |
Super-resolution microscopy based on fluorescence emission difference of cylindrical vector beams |
| url |
http://dx.doi.org/10.1016/j.optcom.2015.05.057 |
| remote_bool |
false |
| author2 |
Kuang, Cuifang Fang, Yue Zhao, Guangyuan Xu, Yingke Liu, Xu |
| author2Str |
Kuang, Cuifang Fang, Yue Zhao, Guangyuan Xu, Yingke Liu, Xu |
| ppnlink |
129361100 |
| mediatype_str_mv |
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| isOA_txt |
false |
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| author2_role |
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| doi_str |
10.1016/j.optcom.2015.05.057 |
| up_date |
2024-07-03T15:51:52.208Z |
| _version_ |
1803573696672038912 |
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| score |
7.402237 |