The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae

Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards ele...
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Autor*in:	Azad, Gajendra Kumar [verfasserIn] Singh, Vikash Baranwal, Shivani Thakare, Mayur Jankiram Tomar, Raghuvir S

Format:	Artikel
Sprache:	Englisch

Erschienen:	2015

Rechteinformationen:	Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Schlagwörter:	Cell wall thickness Cell wall perturbing agent Slt2p-phosphorylation Cell wall integrity pathway Yeast repressor activator protein Cell wall Cell Wall - ultrastructure Phosphorylation - physiology Saccharomyces cerevisiae - metabolism Mitogen-Activated Protein Kinases - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - ultrastructure Cell Wall - metabolism Cell Wall - genetics Saccharomyces cerevisiae Proteins - metabolism Mitogen-Activated Protein Kinases - metabolism

Übergeordnetes Werk:	Enthalten in: FEBS letters - Amsterdam [u.a.] : Elsevier, 1968, 589(2015), 1, Seite 59-67
Übergeordnetes Werk:	volume:589 ; year:2015 ; number:1 ; pages:59-67

Links:	Volltext Link aufrufen Link aufrufen

DOI / URN:	10.1016/j.febslet.2014.11.024

Katalog-ID:	OLC1965544487

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245	1	4	\|a The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae
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520			\|a Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes.
540			\|a Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies
540			\|a Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
650		4	\|a Cell wall thickness
650		4	\|a Cell wall perturbing agent
650		4	\|a Slt2p-phosphorylation
650		4	\|a Cell wall integrity pathway
650		4	\|a Yeast repressor activator protein
650		4	\|a Cell wall
650		4	\|a Cell Wall - ultrastructure
650		4	\|a Phosphorylation - physiology
650		4	\|a Saccharomyces cerevisiae - metabolism
650		4	\|a Mitogen-Activated Protein Kinases - genetics
650		4	\|a Saccharomyces cerevisiae Proteins - genetics
650		4	\|a Saccharomyces cerevisiae - genetics
650		4	\|a Saccharomyces cerevisiae - ultrastructure
650		4	\|a Cell Wall - metabolism
650		4	\|a Cell Wall - genetics
650		4	\|a Saccharomyces cerevisiae Proteins - metabolism
650		4	\|a Mitogen-Activated Protein Kinases - metabolism
700	1		\|a Singh, Vikash \|4 oth
700	1		\|a Baranwal, Shivani \|4 oth
700	1		\|a Thakare, Mayur Jankiram \|4 oth
700	1		\|a Tomar, Raghuvir S \|4 oth
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856	4	2	\|u http://www.ncbi.nlm.nih.gov/pubmed/25481258
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hierarchy_sort_str	2015
publishDate	2015
allfields	10.1016/j.febslet.2014.11.024 doi PQ20160617 (DE-627)OLC1965544487 (DE-599)GBVOLC1965544487 (PRQ)p2011-5e66190356e7a21bc696a2950fa2cb05d482b5a082f132917c9f98b56daaa6cb0 (KEY)0045922420150000589000100059transcriptionfactorrap1pisrequiredfortolerancetoce DE-627 ger DE-627 rakwb eng 570 530 610 DNB Azad, Gajendra Kumar verfasserin aut The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Cell wall thickness Cell wall perturbing agent Slt2p-phosphorylation Cell wall integrity pathway Yeast repressor activator protein Cell wall Cell Wall - ultrastructure Phosphorylation - physiology Saccharomyces cerevisiae - metabolism Mitogen-Activated Protein Kinases - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - ultrastructure Cell Wall - metabolism Cell Wall - genetics Saccharomyces cerevisiae Proteins - metabolism Mitogen-Activated Protein Kinases - metabolism Singh, Vikash oth Baranwal, Shivani oth Thakare, Mayur Jankiram oth Tomar, Raghuvir S oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 1, Seite 59-67 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:1 pages:59-67 http://dx.doi.org/10.1016/j.febslet.2014.11.024 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.11.024/abstract http://www.ncbi.nlm.nih.gov/pubmed/25481258 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 1 59-67
spelling	10.1016/j.febslet.2014.11.024 doi PQ20160617 (DE-627)OLC1965544487 (DE-599)GBVOLC1965544487 (PRQ)p2011-5e66190356e7a21bc696a2950fa2cb05d482b5a082f132917c9f98b56daaa6cb0 (KEY)0045922420150000589000100059transcriptionfactorrap1pisrequiredfortolerancetoce DE-627 ger DE-627 rakwb eng 570 530 610 DNB Azad, Gajendra Kumar verfasserin aut The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Cell wall thickness Cell wall perturbing agent Slt2p-phosphorylation Cell wall integrity pathway Yeast repressor activator protein Cell wall Cell Wall - ultrastructure Phosphorylation - physiology Saccharomyces cerevisiae - metabolism Mitogen-Activated Protein Kinases - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - ultrastructure Cell Wall - metabolism Cell Wall - genetics Saccharomyces cerevisiae Proteins - metabolism Mitogen-Activated Protein Kinases - metabolism Singh, Vikash oth Baranwal, Shivani oth Thakare, Mayur Jankiram oth Tomar, Raghuvir S oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 1, Seite 59-67 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:1 pages:59-67 http://dx.doi.org/10.1016/j.febslet.2014.11.024 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.11.024/abstract http://www.ncbi.nlm.nih.gov/pubmed/25481258 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 1 59-67
allfields_unstemmed	10.1016/j.febslet.2014.11.024 doi PQ20160617 (DE-627)OLC1965544487 (DE-599)GBVOLC1965544487 (PRQ)p2011-5e66190356e7a21bc696a2950fa2cb05d482b5a082f132917c9f98b56daaa6cb0 (KEY)0045922420150000589000100059transcriptionfactorrap1pisrequiredfortolerancetoce DE-627 ger DE-627 rakwb eng 570 530 610 DNB Azad, Gajendra Kumar verfasserin aut The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Cell wall thickness Cell wall perturbing agent Slt2p-phosphorylation Cell wall integrity pathway Yeast repressor activator protein Cell wall Cell Wall - ultrastructure Phosphorylation - physiology Saccharomyces cerevisiae - metabolism Mitogen-Activated Protein Kinases - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - ultrastructure Cell Wall - metabolism Cell Wall - genetics Saccharomyces cerevisiae Proteins - metabolism Mitogen-Activated Protein Kinases - metabolism Singh, Vikash oth Baranwal, Shivani oth Thakare, Mayur Jankiram oth Tomar, Raghuvir S oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 1, Seite 59-67 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:1 pages:59-67 http://dx.doi.org/10.1016/j.febslet.2014.11.024 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.11.024/abstract http://www.ncbi.nlm.nih.gov/pubmed/25481258 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 1 59-67
allfieldsGer	10.1016/j.febslet.2014.11.024 doi PQ20160617 (DE-627)OLC1965544487 (DE-599)GBVOLC1965544487 (PRQ)p2011-5e66190356e7a21bc696a2950fa2cb05d482b5a082f132917c9f98b56daaa6cb0 (KEY)0045922420150000589000100059transcriptionfactorrap1pisrequiredfortolerancetoce DE-627 ger DE-627 rakwb eng 570 530 610 DNB Azad, Gajendra Kumar verfasserin aut The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Cell wall thickness Cell wall perturbing agent Slt2p-phosphorylation Cell wall integrity pathway Yeast repressor activator protein Cell wall Cell Wall - ultrastructure Phosphorylation - physiology Saccharomyces cerevisiae - metabolism Mitogen-Activated Protein Kinases - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - ultrastructure Cell Wall - metabolism Cell Wall - genetics Saccharomyces cerevisiae Proteins - metabolism Mitogen-Activated Protein Kinases - metabolism Singh, Vikash oth Baranwal, Shivani oth Thakare, Mayur Jankiram oth Tomar, Raghuvir S oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 1, Seite 59-67 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:1 pages:59-67 http://dx.doi.org/10.1016/j.febslet.2014.11.024 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.11.024/abstract http://www.ncbi.nlm.nih.gov/pubmed/25481258 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 1 59-67
allfieldsSound	10.1016/j.febslet.2014.11.024 doi PQ20160617 (DE-627)OLC1965544487 (DE-599)GBVOLC1965544487 (PRQ)p2011-5e66190356e7a21bc696a2950fa2cb05d482b5a082f132917c9f98b56daaa6cb0 (KEY)0045922420150000589000100059transcriptionfactorrap1pisrequiredfortolerancetoce DE-627 ger DE-627 rakwb eng 570 530 610 DNB Azad, Gajendra Kumar verfasserin aut The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Cell wall thickness Cell wall perturbing agent Slt2p-phosphorylation Cell wall integrity pathway Yeast repressor activator protein Cell wall Cell Wall - ultrastructure Phosphorylation - physiology Saccharomyces cerevisiae - metabolism Mitogen-Activated Protein Kinases - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - ultrastructure Cell Wall - metabolism Cell Wall - genetics Saccharomyces cerevisiae Proteins - metabolism Mitogen-Activated Protein Kinases - metabolism Singh, Vikash oth Baranwal, Shivani oth Thakare, Mayur Jankiram oth Tomar, Raghuvir S oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 1, Seite 59-67 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:1 pages:59-67 http://dx.doi.org/10.1016/j.febslet.2014.11.024 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.11.024/abstract http://www.ncbi.nlm.nih.gov/pubmed/25481258 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 1 59-67
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topic_facet	Cell wall thickness Cell wall perturbing agent Slt2p-phosphorylation Cell wall integrity pathway Yeast repressor activator protein Cell wall Cell Wall - ultrastructure Phosphorylation - physiology Saccharomyces cerevisiae - metabolism Mitogen-Activated Protein Kinases - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - ultrastructure Cell Wall - metabolism Cell Wall - genetics Saccharomyces cerevisiae Proteins - metabolism Mitogen-Activated Protein Kinases - metabolism
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authorswithroles_txt_mv	Azad, Gajendra Kumar @@aut@@ Singh, Vikash @@oth@@ Baranwal, Shivani @@oth@@ Thakare, Mayur Jankiram @@oth@@ Tomar, Raghuvir S @@oth@@
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author	Azad, Gajendra Kumar
spellingShingle	Azad, Gajendra Kumar ddc 570 misc Cell wall thickness misc Cell wall perturbing agent misc Slt2p-phosphorylation misc Cell wall integrity pathway misc Yeast repressor activator protein misc Cell wall misc Cell Wall - ultrastructure misc Phosphorylation - physiology misc Saccharomyces cerevisiae - metabolism misc Mitogen-Activated Protein Kinases - genetics misc Saccharomyces cerevisiae Proteins - genetics misc Saccharomyces cerevisiae - genetics misc Saccharomyces cerevisiae - ultrastructure misc Cell Wall - metabolism misc Cell Wall - genetics misc Saccharomyces cerevisiae Proteins - metabolism misc Mitogen-Activated Protein Kinases - metabolism The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae
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topic_title	570 530 610 DNB The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae Cell wall thickness Cell wall perturbing agent Slt2p-phosphorylation Cell wall integrity pathway Yeast repressor activator protein Cell wall Cell Wall - ultrastructure Phosphorylation - physiology Saccharomyces cerevisiae - metabolism Mitogen-Activated Protein Kinases - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - ultrastructure Cell Wall - metabolism Cell Wall - genetics Saccharomyces cerevisiae Proteins - metabolism Mitogen-Activated Protein Kinases - metabolism
topic	ddc 570 misc Cell wall thickness misc Cell wall perturbing agent misc Slt2p-phosphorylation misc Cell wall integrity pathway misc Yeast repressor activator protein misc Cell wall misc Cell Wall - ultrastructure misc Phosphorylation - physiology misc Saccharomyces cerevisiae - metabolism misc Mitogen-Activated Protein Kinases - genetics misc Saccharomyces cerevisiae Proteins - genetics misc Saccharomyces cerevisiae - genetics misc Saccharomyces cerevisiae - ultrastructure misc Cell Wall - metabolism misc Cell Wall - genetics misc Saccharomyces cerevisiae Proteins - metabolism misc Mitogen-Activated Protein Kinases - metabolism
topic_unstemmed	ddc 570 misc Cell wall thickness misc Cell wall perturbing agent misc Slt2p-phosphorylation misc Cell wall integrity pathway misc Yeast repressor activator protein misc Cell wall misc Cell Wall - ultrastructure misc Phosphorylation - physiology misc Saccharomyces cerevisiae - metabolism misc Mitogen-Activated Protein Kinases - genetics misc Saccharomyces cerevisiae Proteins - genetics misc Saccharomyces cerevisiae - genetics misc Saccharomyces cerevisiae - ultrastructure misc Cell Wall - metabolism misc Cell Wall - genetics misc Saccharomyces cerevisiae Proteins - metabolism misc Mitogen-Activated Protein Kinases - metabolism
topic_browse	ddc 570 misc Cell wall thickness misc Cell wall perturbing agent misc Slt2p-phosphorylation misc Cell wall integrity pathway misc Yeast repressor activator protein misc Cell wall misc Cell Wall - ultrastructure misc Phosphorylation - physiology misc Saccharomyces cerevisiae - metabolism misc Mitogen-Activated Protein Kinases - genetics misc Saccharomyces cerevisiae Proteins - genetics misc Saccharomyces cerevisiae - genetics misc Saccharomyces cerevisiae - ultrastructure misc Cell Wall - metabolism misc Cell Wall - genetics misc Saccharomyces cerevisiae Proteins - metabolism misc Mitogen-Activated Protein Kinases - metabolism
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title	The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae
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title_full	The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae
author_sort	Azad, Gajendra Kumar
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title_sort	transcription factor rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in saccharomyces cerevisiae
title_auth	The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae
abstract	Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes.
abstractGer	Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes.
abstract_unstemmed	Yeast repressor activator protein (Rap1p) is involved in genomic stability and transcriptional regulation. We explored the function of Rap1p in yeast physiology using Rap1p truncation mutants. Our results revealed that the N‐terminal truncation of Rap1p (Rap1ΔN) leads to hypersensitivity towards elevated temperature and cell‐wall perturbing agents. Cell wall analysis showed an increase in the chitin and glucan content in Rap1ΔN cells as compared with wild type cells. Accordingly, mutant cells had a twofold thicker cell wall, as observed by electron microscopy. Furthermore, Rap1ΔN cells had increased levels of phosphorylated Slt2p, a MAP kinase of the cell wall integrity pathway. Mutant cells also had elevated levels of cell wall integrity response transcripts. Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes.
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title_short	The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae
url	http://dx.doi.org/10.1016/j.febslet.2014.11.024 http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.11.024/abstract http://www.ncbi.nlm.nih.gov/pubmed/25481258
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author2	Singh, Vikash Baranwal, Shivani Thakare, Mayur Jankiram Tomar, Raghuvir S
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up_date	2024-07-03T18:12:39.213Z
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Taken together, our findings suggest a connection between Rap1p and cell wall homeostasis. • Rap1p provides tolerance to heat and cell wall perturbing agents. • Removal of N terminal domain of Rap1 (Rap1ΔN) causes cell aggregation phenotype. • Rap1ΔN cells exhibits increase in thickness of cell wall. • Slt2p remains activated in Rap1ΔN mutant. • Rap1ΔN shows increase in expression of cell wall damage response genes.</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. 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genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Saccharomyces cerevisiae Proteins - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Saccharomyces cerevisiae - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Saccharomyces cerevisiae - ultrastructure</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Wall - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Wall - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Saccharomyces cerevisiae Proteins - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mitogen-Activated Protein Kinases - metabolism</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Singh, Vikash</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Baranwal, Shivani</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Thakare, Mayur Jankiram</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tomar, Raghuvir S</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">FEBS letters</subfield><subfield code="d">Amsterdam [u.a.] : Elsevier, 1968</subfield><subfield code="g">589(2015), 1, Seite 59-67</subfield><subfield code="w">(DE-627)129522023</subfield><subfield code="w">(DE-600)212746-5</subfield><subfield code="w">(DE-576)014938014</subfield><subfield code="x">0014-5793</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:589</subfield><subfield code="g">year:2015</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:59-67</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://dx.doi.org/10.1016/j.febslet.2014.11.024</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2014.11.024/abstract</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://www.ncbi.nlm.nih.gov/pubmed/25481258</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHY</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_21</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_211</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2219</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4219</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">589</subfield><subfield code="j">2015</subfield><subfield code="e">1</subfield><subfield code="h">59-67</subfield></datafield></record></collection>
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The transcription factor Rap1p is required for tolerance to cell‐wall perturbing agents and for cell‐wall maintenance in Saccharomyces cerevisiae

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