Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module

Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α...
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Autor*in:	Hernandez-Gomez, Mercedes C [verfasserIn] Rydahl, Maja G Rogowski, Artur Morland, Carl Cartmell, Alan Crouch, Lucy Labourel, Aurore Fontes, Carlos M.G.A Willats, William G.T Gilbert, Harry J Knox, J. Paul

Format:	Artikel
Sprache:	Englisch

Erschienen:	2015

Rechteinformationen:	Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. © COPYRIGHT 2015 Elsevier B.V.

Schlagwörter:	CBM3a CBM Crystalline cellulose ITC Plant cell wall carbohydrate-binding module isothermal titration calorimetry xyloglucan Cellulose - chemistry Xylans - chemistry Bacterial Proteins - chemistry Xylans - metabolism Bacterial Proteins - metabolism Glucans - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Cell Wall - metabolism Glucans - chemistry Cellulose - metabolism Chemical properties Cellulose

Übergeordnetes Werk:	Enthalten in: FEBS letters - Amsterdam [u.a.] : Elsevier, 1968, 589(2015), 18, Seite 2297-2303
Übergeordnetes Werk:	volume:589 ; year:2015 ; number:18 ; pages:2297-2303

Links:	Volltext Link aufrufen Link aufrufen

DOI / URN:	10.1016/j.febslet.2015.07.009

Katalog-ID:	OLC1965547435

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520			\|a Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a.
540			\|a Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies
540			\|a Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
540			\|a © COPYRIGHT 2015 Elsevier B.V.
650		4	\|a CBM3a
650		4	\|a CBM
650		4	\|a Crystalline cellulose
650		4	\|a ITC
650		4	\|a Plant cell wall
650		4	\|a carbohydrate-binding module
650		4	\|a isothermal titration calorimetry
650		4	\|a xyloglucan
650		4	\|a Cellulose - chemistry
650		4	\|a Xylans - chemistry
650		4	\|a Bacterial Proteins - chemistry
650		4	\|a Xylans - metabolism
650		4	\|a Bacterial Proteins - metabolism
650		4	\|a Glucans - metabolism
650		4	\|a Membrane Proteins - chemistry
650		4	\|a Membrane Proteins - metabolism
650		4	\|a Cell Wall - metabolism
650		4	\|a Glucans - chemistry
650		4	\|a Cellulose - metabolism
650		4	\|a Chemical properties
650		4	\|a Cellulose
700	1		\|a Rydahl, Maja G \|4 oth
700	1		\|a Rogowski, Artur \|4 oth
700	1		\|a Morland, Carl \|4 oth
700	1		\|a Cartmell, Alan \|4 oth
700	1		\|a Crouch, Lucy \|4 oth
700	1		\|a Labourel, Aurore \|4 oth
700	1		\|a Fontes, Carlos M.G.A \|4 oth
700	1		\|a Willats, William G.T \|4 oth
700	1		\|a Gilbert, Harry J \|4 oth
700	1		\|a Knox, J. Paul \|4 oth
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856	4	1	\|u http://dx.doi.org/10.1016/j.febslet.2015.07.009 \|3 Volltext
856	4	2	\|u http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.07.009/abstract
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Indexfelder

author_variant	m c h g mch mchg
matchkey_str	article:00145793:2015----::eontooxlguabtersalncluoeidnstoaaiy
hierarchy_sort_str	2015
publishDate	2015
allfields	10.1016/j.febslet.2015.07.009 doi PQ20160617 (DE-627)OLC1965547435 (DE-599)GBVOLC1965547435 (PRQ)c2178-d591ed1c6ad95fd9670f02233d28f31f251e5c131b6edd0ab6def4ae525c7ecb0 (KEY)0045922420150000589001802297recognitionofxyloglucanbythecrystallinecellulosebi DE-627 ger DE-627 rakwb eng 570 530 610 DNB Hernandez-Gomez, Mercedes C verfasserin aut Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. © COPYRIGHT 2015 Elsevier B.V. CBM3a CBM Crystalline cellulose ITC Plant cell wall carbohydrate-binding module isothermal titration calorimetry xyloglucan Cellulose - chemistry Xylans - chemistry Bacterial Proteins - chemistry Xylans - metabolism Bacterial Proteins - metabolism Glucans - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Cell Wall - metabolism Glucans - chemistry Cellulose - metabolism Chemical properties Cellulose Rydahl, Maja G oth Rogowski, Artur oth Morland, Carl oth Cartmell, Alan oth Crouch, Lucy oth Labourel, Aurore oth Fontes, Carlos M.G.A oth Willats, William G.T oth Gilbert, Harry J oth Knox, J. Paul oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 18, Seite 2297-2303 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:18 pages:2297-2303 http://dx.doi.org/10.1016/j.febslet.2015.07.009 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.07.009/abstract http://www.ncbi.nlm.nih.gov/pubmed/26193423 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 18 2297-2303
spelling	10.1016/j.febslet.2015.07.009 doi PQ20160617 (DE-627)OLC1965547435 (DE-599)GBVOLC1965547435 (PRQ)c2178-d591ed1c6ad95fd9670f02233d28f31f251e5c131b6edd0ab6def4ae525c7ecb0 (KEY)0045922420150000589001802297recognitionofxyloglucanbythecrystallinecellulosebi DE-627 ger DE-627 rakwb eng 570 530 610 DNB Hernandez-Gomez, Mercedes C verfasserin aut Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. © COPYRIGHT 2015 Elsevier B.V. CBM3a CBM Crystalline cellulose ITC Plant cell wall carbohydrate-binding module isothermal titration calorimetry xyloglucan Cellulose - chemistry Xylans - chemistry Bacterial Proteins - chemistry Xylans - metabolism Bacterial Proteins - metabolism Glucans - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Cell Wall - metabolism Glucans - chemistry Cellulose - metabolism Chemical properties Cellulose Rydahl, Maja G oth Rogowski, Artur oth Morland, Carl oth Cartmell, Alan oth Crouch, Lucy oth Labourel, Aurore oth Fontes, Carlos M.G.A oth Willats, William G.T oth Gilbert, Harry J oth Knox, J. Paul oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 18, Seite 2297-2303 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:18 pages:2297-2303 http://dx.doi.org/10.1016/j.febslet.2015.07.009 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.07.009/abstract http://www.ncbi.nlm.nih.gov/pubmed/26193423 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 18 2297-2303
allfields_unstemmed	10.1016/j.febslet.2015.07.009 doi PQ20160617 (DE-627)OLC1965547435 (DE-599)GBVOLC1965547435 (PRQ)c2178-d591ed1c6ad95fd9670f02233d28f31f251e5c131b6edd0ab6def4ae525c7ecb0 (KEY)0045922420150000589001802297recognitionofxyloglucanbythecrystallinecellulosebi DE-627 ger DE-627 rakwb eng 570 530 610 DNB Hernandez-Gomez, Mercedes C verfasserin aut Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. © COPYRIGHT 2015 Elsevier B.V. CBM3a CBM Crystalline cellulose ITC Plant cell wall carbohydrate-binding module isothermal titration calorimetry xyloglucan Cellulose - chemistry Xylans - chemistry Bacterial Proteins - chemistry Xylans - metabolism Bacterial Proteins - metabolism Glucans - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Cell Wall - metabolism Glucans - chemistry Cellulose - metabolism Chemical properties Cellulose Rydahl, Maja G oth Rogowski, Artur oth Morland, Carl oth Cartmell, Alan oth Crouch, Lucy oth Labourel, Aurore oth Fontes, Carlos M.G.A oth Willats, William G.T oth Gilbert, Harry J oth Knox, J. Paul oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 18, Seite 2297-2303 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:18 pages:2297-2303 http://dx.doi.org/10.1016/j.febslet.2015.07.009 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.07.009/abstract http://www.ncbi.nlm.nih.gov/pubmed/26193423 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 18 2297-2303
allfieldsGer	10.1016/j.febslet.2015.07.009 doi PQ20160617 (DE-627)OLC1965547435 (DE-599)GBVOLC1965547435 (PRQ)c2178-d591ed1c6ad95fd9670f02233d28f31f251e5c131b6edd0ab6def4ae525c7ecb0 (KEY)0045922420150000589001802297recognitionofxyloglucanbythecrystallinecellulosebi DE-627 ger DE-627 rakwb eng 570 530 610 DNB Hernandez-Gomez, Mercedes C verfasserin aut Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. © COPYRIGHT 2015 Elsevier B.V. CBM3a CBM Crystalline cellulose ITC Plant cell wall carbohydrate-binding module isothermal titration calorimetry xyloglucan Cellulose - chemistry Xylans - chemistry Bacterial Proteins - chemistry Xylans - metabolism Bacterial Proteins - metabolism Glucans - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Cell Wall - metabolism Glucans - chemistry Cellulose - metabolism Chemical properties Cellulose Rydahl, Maja G oth Rogowski, Artur oth Morland, Carl oth Cartmell, Alan oth Crouch, Lucy oth Labourel, Aurore oth Fontes, Carlos M.G.A oth Willats, William G.T oth Gilbert, Harry J oth Knox, J. Paul oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 18, Seite 2297-2303 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:18 pages:2297-2303 http://dx.doi.org/10.1016/j.febslet.2015.07.009 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.07.009/abstract http://www.ncbi.nlm.nih.gov/pubmed/26193423 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 18 2297-2303
allfieldsSound	10.1016/j.febslet.2015.07.009 doi PQ20160617 (DE-627)OLC1965547435 (DE-599)GBVOLC1965547435 (PRQ)c2178-d591ed1c6ad95fd9670f02233d28f31f251e5c131b6edd0ab6def4ae525c7ecb0 (KEY)0045922420150000589001802297recognitionofxyloglucanbythecrystallinecellulosebi DE-627 ger DE-627 rakwb eng 570 530 610 DNB Hernandez-Gomez, Mercedes C verfasserin aut Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a. Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. © COPYRIGHT 2015 Elsevier B.V. CBM3a CBM Crystalline cellulose ITC Plant cell wall carbohydrate-binding module isothermal titration calorimetry xyloglucan Cellulose - chemistry Xylans - chemistry Bacterial Proteins - chemistry Xylans - metabolism Bacterial Proteins - metabolism Glucans - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Cell Wall - metabolism Glucans - chemistry Cellulose - metabolism Chemical properties Cellulose Rydahl, Maja G oth Rogowski, Artur oth Morland, Carl oth Cartmell, Alan oth Crouch, Lucy oth Labourel, Aurore oth Fontes, Carlos M.G.A oth Willats, William G.T oth Gilbert, Harry J oth Knox, J. Paul oth Enthalten in FEBS letters Amsterdam [u.a.] : Elsevier, 1968 589(2015), 18, Seite 2297-2303 (DE-627)129522023 (DE-600)212746-5 (DE-576)014938014 0014-5793 nnns volume:589 year:2015 number:18 pages:2297-2303 http://dx.doi.org/10.1016/j.febslet.2015.07.009 Volltext http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.07.009/abstract http://www.ncbi.nlm.nih.gov/pubmed/26193423 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 AR 589 2015 18 2297-2303
language	English
source	Enthalten in FEBS letters 589(2015), 18, Seite 2297-2303 volume:589 year:2015 number:18 pages:2297-2303
sourceStr	Enthalten in FEBS letters 589(2015), 18, Seite 2297-2303 volume:589 year:2015 number:18 pages:2297-2303
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CBM3a CBM Crystalline cellulose ITC Plant cell wall carbohydrate-binding module isothermal titration calorimetry xyloglucan Cellulose - chemistry Xylans - chemistry Bacterial Proteins - chemistry Xylans - metabolism Bacterial Proteins - metabolism Glucans - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Cell Wall - metabolism Glucans - chemistry Cellulose - metabolism Chemical properties Cellulose
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authorswithroles_txt_mv	Hernandez-Gomez, Mercedes C @@aut@@ Rydahl, Maja G @@oth@@ Rogowski, Artur @@oth@@ Morland, Carl @@oth@@ Cartmell, Alan @@oth@@ Crouch, Lucy @@oth@@ Labourel, Aurore @@oth@@ Fontes, Carlos M.G.A @@oth@@ Willats, William G.T @@oth@@ Gilbert, Harry J @@oth@@ Knox, J. Paul @@oth@@
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author	Hernandez-Gomez, Mercedes C
spellingShingle	Hernandez-Gomez, Mercedes C ddc 570 misc CBM3a misc CBM misc Crystalline cellulose misc ITC misc Plant cell wall misc carbohydrate-binding module misc isothermal titration calorimetry misc xyloglucan misc Cellulose - chemistry misc Xylans - chemistry misc Bacterial Proteins - chemistry misc Xylans - metabolism misc Bacterial Proteins - metabolism misc Glucans - metabolism misc Membrane Proteins - chemistry misc Membrane Proteins - metabolism misc Cell Wall - metabolism misc Glucans - chemistry misc Cellulose - metabolism misc Chemical properties misc Cellulose Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module
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topic_title	570 530 610 DNB Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module CBM3a CBM Crystalline cellulose ITC Plant cell wall carbohydrate-binding module isothermal titration calorimetry xyloglucan Cellulose - chemistry Xylans - chemistry Bacterial Proteins - chemistry Xylans - metabolism Bacterial Proteins - metabolism Glucans - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Cell Wall - metabolism Glucans - chemistry Cellulose - metabolism Chemical properties Cellulose
topic	ddc 570 misc CBM3a misc CBM misc Crystalline cellulose misc ITC misc Plant cell wall misc carbohydrate-binding module misc isothermal titration calorimetry misc xyloglucan misc Cellulose - chemistry misc Xylans - chemistry misc Bacterial Proteins - chemistry misc Xylans - metabolism misc Bacterial Proteins - metabolism misc Glucans - metabolism misc Membrane Proteins - chemistry misc Membrane Proteins - metabolism misc Cell Wall - metabolism misc Glucans - chemistry misc Cellulose - metabolism misc Chemical properties misc Cellulose
topic_unstemmed	ddc 570 misc CBM3a misc CBM misc Crystalline cellulose misc ITC misc Plant cell wall misc carbohydrate-binding module misc isothermal titration calorimetry misc xyloglucan misc Cellulose - chemistry misc Xylans - chemistry misc Bacterial Proteins - chemistry misc Xylans - metabolism misc Bacterial Proteins - metabolism misc Glucans - metabolism misc Membrane Proteins - chemistry misc Membrane Proteins - metabolism misc Cell Wall - metabolism misc Glucans - chemistry misc Cellulose - metabolism misc Chemical properties misc Cellulose
topic_browse	ddc 570 misc CBM3a misc CBM misc Crystalline cellulose misc ITC misc Plant cell wall misc carbohydrate-binding module misc isothermal titration calorimetry misc xyloglucan misc Cellulose - chemistry misc Xylans - chemistry misc Bacterial Proteins - chemistry misc Xylans - metabolism misc Bacterial Proteins - metabolism misc Glucans - metabolism misc Membrane Proteins - chemistry misc Membrane Proteins - metabolism misc Cell Wall - metabolism misc Glucans - chemistry misc Cellulose - metabolism misc Chemical properties misc Cellulose
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title	Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module
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title_full	Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module
author_sort	Hernandez-Gomez, Mercedes C
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author-letter	Hernandez-Gomez, Mercedes C
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title_sort	recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module
title_auth	Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module
abstract	Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a.
abstractGer	Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a.
abstract_unstemmed	Type A non‐catalytic carbohydrate‐binding modules (CBMs), exemplified by Ct CBM3a cipA , are widely believed to specifically target crystalline cellulose through entropic forces. Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_70 GBV_ILN_211 GBV_ILN_2219 GBV_ILN_4012 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305
container_issue	18
title_short	Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module
url	http://dx.doi.org/10.1016/j.febslet.2015.07.009 http://onlinelibrary.wiley.com/doi/10.1016/j.febslet.2015.07.009/abstract http://www.ncbi.nlm.nih.gov/pubmed/26193423
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author2	Rydahl, Maja G Rogowski, Artur Morland, Carl Cartmell, Alan Crouch, Lucy Labourel, Aurore Fontes, Carlos M.G.A Willats, William G.T Gilbert, Harry J Knox, J. Paul
author2Str	Rydahl, Maja G Rogowski, Artur Morland, Carl Cartmell, Alan Crouch, Lucy Labourel, Aurore Fontes, Carlos M.G.A Willats, William G.T Gilbert, Harry J Knox, J. Paul
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up_date	2024-07-03T18:13:47.129Z
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Here we have tested the hypothesis that type A CBMs can also bind to xyloglucan (XG), a soluble β‐1,4‐glucan containing α‐1,6‐xylose side chains. Ct CBM3a cipA bound to xyloglucan in cell walls and arrayed on solid surfaces. Xyloglucan and cellulose were shown to bind to the same planar surface on CBM3a cipA . A range of type A CBMs from different families were shown to bind to xyloglucan in solution with ligand binding driven by enthalpic changes. The nature of CBM‐polysaccharide interactions is discussed. Ct CBM3a can bind to xyloglucan in addition to crystalline cellulose. Five point mutations at the Ct CBM3a crystalline cellulose binding site abolished recognition of both crystalline cellulose and xyloglucan. XG polysaccharides, but not XG‐derived oligosaccharides, in solution can be bound by Ct CBM3a.</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Nutzungsrecht: FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">© COPYRIGHT 2015 Elsevier B.V.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CBM3a</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CBM</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Crystalline cellulose</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ITC</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Plant cell wall</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">carbohydrate-binding module</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">isothermal titration calorimetry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">xyloglucan</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cellulose - chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Xylans - chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacterial Proteins - chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Xylans - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacterial Proteins - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Glucans - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Membrane Proteins - chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Membrane Proteins - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Wall - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Glucans - chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cellulose - metabolism</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chemical properties</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cellulose</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rydahl, Maja G</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rogowski, Artur</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Morland, Carl</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cartmell, Alan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Crouch, Lucy</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Labourel, Aurore</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fontes, Carlos M.G.A</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Willats, William G.T</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gilbert, Harry J</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Knox, J. 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Recognition of xyloglucan by the crystalline cellulose‐binding site of a family 3a carbohydrate‐binding module

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