Mass spectrometry imaging with laser-induced postionization

Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable...
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Autor*in:	Jens Soltwisch [verfasserIn] Hans Kettling Simeon Vens-Cappell Marcel Wiegelmann Johannes Müthing Klaus Dreisewerd

Format:	Artikel
Sprache:	Englisch

Erschienen:	2015

Rechteinformationen:	Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science.

Schlagwörter:	Carbohydrates - analysis Membrane Lipids - analysis Carbohydrates - chemistry Vitamins - analysis Seminiferous Tubules - chemistry Gangliosides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Malus - chemistry Lipids - chemistry Lipids - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Cerebellum - chemistry Membrane Lipids - chemistry Gangliosides - chemistry Vitamins - chemistry

Übergeordnetes Werk:	Enthalten in: Science - Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883, 348(2015), 6231, Seite 211-215
Übergeordnetes Werk:	volume:348 ; year:2015 ; number:6231 ; pages:211-215

Links:	Volltext Link aufrufen Link aufrufen

DOI / URN:	10.1126/science.aaa1051

Katalog-ID:	OLC1966820992

Internformat


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520			\|a Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range.
540			\|a Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science.
650		4	\|a Carbohydrates - analysis
650		4	\|a Membrane Lipids - analysis
650		4	\|a Carbohydrates - chemistry
650		4	\|a Vitamins - analysis
650		4	\|a Seminiferous Tubules - chemistry
650		4	\|a Gangliosides - analysis
650		4	\|a Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
650		4	\|a Malus - chemistry
650		4	\|a Lipids - chemistry
650		4	\|a Lipids - analysis
650		4	\|a Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation
650		4	\|a Cerebellum - chemistry
650		4	\|a Membrane Lipids - chemistry
650		4	\|a Gangliosides - chemistry
650		4	\|a Vitamins - chemistry
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700	0		\|a Klaus Dreisewerd \|4 oth
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allfields	10.1126/science.aaa1051 doi PQ20160617 (DE-627)OLC1966820992 (DE-599)GBVOLC1966820992 (PRQ)c2753-cafd51e72acf1f3904726ae162c9cef9c210037302ca1062021ff51e52d8cb220 (KEY)0063888920150000348623100211massspectrometryimagingwithlaserinducedpostionizat DE-627 ger DE-627 rakwb eng 500 DNB LING fid Jens Soltwisch verfasserin aut Mass spectrometry imaging with laser-induced postionization 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. Carbohydrates - analysis Membrane Lipids - analysis Carbohydrates - chemistry Vitamins - analysis Seminiferous Tubules - chemistry Gangliosides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Malus - chemistry Lipids - chemistry Lipids - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Cerebellum - chemistry Membrane Lipids - chemistry Gangliosides - chemistry Vitamins - chemistry Hans Kettling oth Simeon Vens-Cappell oth Marcel Wiegelmann oth Johannes Müthing oth Klaus Dreisewerd oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 348(2015), 6231, Seite 211-215 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:348 year:2015 number:6231 pages:211-215 http://dx.doi.org/10.1126/science.aaa1051 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25745064 http://search.proquest.com/docview/1672088647 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 348 2015 6231 211-215
spelling	10.1126/science.aaa1051 doi PQ20160617 (DE-627)OLC1966820992 (DE-599)GBVOLC1966820992 (PRQ)c2753-cafd51e72acf1f3904726ae162c9cef9c210037302ca1062021ff51e52d8cb220 (KEY)0063888920150000348623100211massspectrometryimagingwithlaserinducedpostionizat DE-627 ger DE-627 rakwb eng 500 DNB LING fid Jens Soltwisch verfasserin aut Mass spectrometry imaging with laser-induced postionization 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. Carbohydrates - analysis Membrane Lipids - analysis Carbohydrates - chemistry Vitamins - analysis Seminiferous Tubules - chemistry Gangliosides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Malus - chemistry Lipids - chemistry Lipids - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Cerebellum - chemistry Membrane Lipids - chemistry Gangliosides - chemistry Vitamins - chemistry Hans Kettling oth Simeon Vens-Cappell oth Marcel Wiegelmann oth Johannes Müthing oth Klaus Dreisewerd oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 348(2015), 6231, Seite 211-215 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:348 year:2015 number:6231 pages:211-215 http://dx.doi.org/10.1126/science.aaa1051 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25745064 http://search.proquest.com/docview/1672088647 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 348 2015 6231 211-215
allfields_unstemmed	10.1126/science.aaa1051 doi PQ20160617 (DE-627)OLC1966820992 (DE-599)GBVOLC1966820992 (PRQ)c2753-cafd51e72acf1f3904726ae162c9cef9c210037302ca1062021ff51e52d8cb220 (KEY)0063888920150000348623100211massspectrometryimagingwithlaserinducedpostionizat DE-627 ger DE-627 rakwb eng 500 DNB LING fid Jens Soltwisch verfasserin aut Mass spectrometry imaging with laser-induced postionization 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. Carbohydrates - analysis Membrane Lipids - analysis Carbohydrates - chemistry Vitamins - analysis Seminiferous Tubules - chemistry Gangliosides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Malus - chemistry Lipids - chemistry Lipids - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Cerebellum - chemistry Membrane Lipids - chemistry Gangliosides - chemistry Vitamins - chemistry Hans Kettling oth Simeon Vens-Cappell oth Marcel Wiegelmann oth Johannes Müthing oth Klaus Dreisewerd oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 348(2015), 6231, Seite 211-215 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:348 year:2015 number:6231 pages:211-215 http://dx.doi.org/10.1126/science.aaa1051 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25745064 http://search.proquest.com/docview/1672088647 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 348 2015 6231 211-215
allfieldsGer	10.1126/science.aaa1051 doi PQ20160617 (DE-627)OLC1966820992 (DE-599)GBVOLC1966820992 (PRQ)c2753-cafd51e72acf1f3904726ae162c9cef9c210037302ca1062021ff51e52d8cb220 (KEY)0063888920150000348623100211massspectrometryimagingwithlaserinducedpostionizat DE-627 ger DE-627 rakwb eng 500 DNB LING fid Jens Soltwisch verfasserin aut Mass spectrometry imaging with laser-induced postionization 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. Carbohydrates - analysis Membrane Lipids - analysis Carbohydrates - chemistry Vitamins - analysis Seminiferous Tubules - chemistry Gangliosides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Malus - chemistry Lipids - chemistry Lipids - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Cerebellum - chemistry Membrane Lipids - chemistry Gangliosides - chemistry Vitamins - chemistry Hans Kettling oth Simeon Vens-Cappell oth Marcel Wiegelmann oth Johannes Müthing oth Klaus Dreisewerd oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 348(2015), 6231, Seite 211-215 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:348 year:2015 number:6231 pages:211-215 http://dx.doi.org/10.1126/science.aaa1051 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25745064 http://search.proquest.com/docview/1672088647 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 348 2015 6231 211-215
allfieldsSound	10.1126/science.aaa1051 doi PQ20160617 (DE-627)OLC1966820992 (DE-599)GBVOLC1966820992 (PRQ)c2753-cafd51e72acf1f3904726ae162c9cef9c210037302ca1062021ff51e52d8cb220 (KEY)0063888920150000348623100211massspectrometryimagingwithlaserinducedpostionizat DE-627 ger DE-627 rakwb eng 500 DNB LING fid Jens Soltwisch verfasserin aut Mass spectrometry imaging with laser-induced postionization 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. Carbohydrates - analysis Membrane Lipids - analysis Carbohydrates - chemistry Vitamins - analysis Seminiferous Tubules - chemistry Gangliosides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Malus - chemistry Lipids - chemistry Lipids - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Cerebellum - chemistry Membrane Lipids - chemistry Gangliosides - chemistry Vitamins - chemistry Hans Kettling oth Simeon Vens-Cappell oth Marcel Wiegelmann oth Johannes Müthing oth Klaus Dreisewerd oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 348(2015), 6231, Seite 211-215 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:348 year:2015 number:6231 pages:211-215 http://dx.doi.org/10.1126/science.aaa1051 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25745064 http://search.proquest.com/docview/1672088647 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 348 2015 6231 211-215
language	English
source	Enthalten in Science 348(2015), 6231, Seite 211-215 volume:348 year:2015 number:6231 pages:211-215
sourceStr	Enthalten in Science 348(2015), 6231, Seite 211-215 volume:348 year:2015 number:6231 pages:211-215
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Carbohydrates - analysis Membrane Lipids - analysis Carbohydrates - chemistry Vitamins - analysis Seminiferous Tubules - chemistry Gangliosides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Malus - chemistry Lipids - chemistry Lipids - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Cerebellum - chemistry Membrane Lipids - chemistry Gangliosides - chemistry Vitamins - chemistry
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container_title	Science
authorswithroles_txt_mv	Jens Soltwisch @@aut@@ Hans Kettling @@oth@@ Simeon Vens-Cappell @@oth@@ Marcel Wiegelmann @@oth@@ Johannes Müthing @@oth@@ Klaus Dreisewerd @@oth@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	12931482X
dewey-sort	3500
id	OLC1966820992
language_de	englisch
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author	Jens Soltwisch
spellingShingle	Jens Soltwisch ddc 500 fid LING misc Carbohydrates - analysis misc Membrane Lipids - analysis misc Carbohydrates - chemistry misc Vitamins - analysis misc Seminiferous Tubules - chemistry misc Gangliosides - analysis misc Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods misc Malus - chemistry misc Lipids - chemistry misc Lipids - analysis misc Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation misc Cerebellum - chemistry misc Membrane Lipids - chemistry misc Gangliosides - chemistry misc Vitamins - chemistry Mass spectrometry imaging with laser-induced postionization
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topic_title	500 DNB LING fid Mass spectrometry imaging with laser-induced postionization Carbohydrates - analysis Membrane Lipids - analysis Carbohydrates - chemistry Vitamins - analysis Seminiferous Tubules - chemistry Gangliosides - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Malus - chemistry Lipids - chemistry Lipids - analysis Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation Cerebellum - chemistry Membrane Lipids - chemistry Gangliosides - chemistry Vitamins - chemistry
topic	ddc 500 fid LING misc Carbohydrates - analysis misc Membrane Lipids - analysis misc Carbohydrates - chemistry misc Vitamins - analysis misc Seminiferous Tubules - chemistry misc Gangliosides - analysis misc Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods misc Malus - chemistry misc Lipids - chemistry misc Lipids - analysis misc Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation misc Cerebellum - chemistry misc Membrane Lipids - chemistry misc Gangliosides - chemistry misc Vitamins - chemistry
topic_unstemmed	ddc 500 fid LING misc Carbohydrates - analysis misc Membrane Lipids - analysis misc Carbohydrates - chemistry misc Vitamins - analysis misc Seminiferous Tubules - chemistry misc Gangliosides - analysis misc Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods misc Malus - chemistry misc Lipids - chemistry misc Lipids - analysis misc Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation misc Cerebellum - chemistry misc Membrane Lipids - chemistry misc Gangliosides - chemistry misc Vitamins - chemistry
topic_browse	ddc 500 fid LING misc Carbohydrates - analysis misc Membrane Lipids - analysis misc Carbohydrates - chemistry misc Vitamins - analysis misc Seminiferous Tubules - chemistry misc Gangliosides - analysis misc Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods misc Malus - chemistry misc Lipids - chemistry misc Lipids - analysis misc Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - instrumentation misc Cerebellum - chemistry misc Membrane Lipids - chemistry misc Gangliosides - chemistry misc Vitamins - chemistry
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title	Mass spectrometry imaging with laser-induced postionization
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title_full	Mass spectrometry imaging with laser-induced postionization
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title_sort	mass spectrometry imaging with laser-induced postionization
title_auth	Mass spectrometry imaging with laser-induced postionization
abstract	Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range.
abstractGer	Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range.
abstract_unstemmed	Chemical imaging of cell membranes can be performed with matrix-assisted laser desorption/ionization mass spectrometry (MALDI), but low ionization efficiency often leads to a signal dominated by the main lipid components, such as abundant phosphatidylcholine species. Soltwisch et al. used a tunable laser for post-ionization of neutral species to boost the signal for other membrane components, such as cholesterol and phospho- and glycolipids. Imaging of cells and tissues with these methods allows differentiation based on a more extensive chemical signature. Science, this issue p. 211 Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) can simultaneously record the lateral distribution of numerous biomolecules in tissue slices, but its sensitivity is restricted by limited ionization. We used a wavelength-tunable postionization laser to initiate secondary MALDI-like ionization processes in the gas phase. In this way, we could increase the ion yields for numerous lipid classes, liposoluble vitamins, and saccharides, imaged in animal and plant tissue with a 5-micrometer-wide laser spot, by up to two orders of magnitude. Critical parameters for initiation of the secondary ionization processes are pressure of the cooling gas in the ion source, laser wavelength, pulse energy, and delay between the two laser pulses. The technology could enable sensitive MALDI-MS imaging with a lateral resolution in the low micrometer range.
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title_short	Mass spectrometry imaging with laser-induced postionization
url	http://dx.doi.org/10.1126/science.aaa1051 http://www.ncbi.nlm.nih.gov/pubmed/25745064 http://search.proquest.com/docview/1672088647
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up_date	2024-07-03T23:01:06.877Z
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Mass spectrometry imaging with laser-induced postionization

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