Soft pinning of liquid domains on topographical hemispherical caps
The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phase...
Ausführliche Beschreibung
Autor*in: |
Feriani, Luigi [verfasserIn] |
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Format: |
Artikel |
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Sprache: |
Englisch |
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2015 |
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Rechteinformationen: |
Nutzungsrecht: Crown Copyright © 2014. Published by Elsevier Ireland Ltd. All rights reserved. |
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Schlagwörter: |
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Übergeordnetes Werk: |
Enthalten in: Chemistry and physics of lipids - Shannon [u.a.] : Elsevier Scientific Publishers, 1966, 185(2015), Seite 78-87 |
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Übergeordnetes Werk: |
volume:185 ; year:2015 ; pages:78-87 |
Links: |
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DOI / URN: |
10.1016/j.chemphyslip.2014.07.012 |
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10.1016/j.chemphyslip.2014.07.012 doi PQ20160617 (DE-627)OLC1966944675 (DE-599)GBVOLC1966944675 (PRQ)c1336-787702bcd39c22c15b0cbab19b3be39dacfdec71f467c3c764731e0472aacc9d0 (KEY)0065792120150000185000000078softpinningofliquiddomainsontopographicalhemispher DE-627 ger DE-627 rakwb eng 570 540 530 DNB Feriani, Luigi verfasserin aut Soft pinning of liquid domains on topographical hemispherical caps 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances. Nutzungsrecht: Crown Copyright © 2014. Published by Elsevier Ireland Ltd. All rights reserved. Cell Membrane - chemistry Dimethylpolysiloxanes - chemistry Lipid Bilayers - chemistry Cristofolini, Luigi oth Cicuta, Pietro oth Enthalten in Chemistry and physics of lipids Shannon [u.a.] : Elsevier Scientific Publishers, 1966 185(2015), Seite 78-87 (DE-627)129526878 (DE-600)213869-4 (DE-576)014946831 0009-3084 nnns volume:185 year:2015 pages:78-87 http://dx.doi.org/10.1016/j.chemphyslip.2014.07.012 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25109251 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 AR 185 2015 78-87 |
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10.1016/j.chemphyslip.2014.07.012 doi PQ20160617 (DE-627)OLC1966944675 (DE-599)GBVOLC1966944675 (PRQ)c1336-787702bcd39c22c15b0cbab19b3be39dacfdec71f467c3c764731e0472aacc9d0 (KEY)0065792120150000185000000078softpinningofliquiddomainsontopographicalhemispher DE-627 ger DE-627 rakwb eng 570 540 530 DNB Feriani, Luigi verfasserin aut Soft pinning of liquid domains on topographical hemispherical caps 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances. Nutzungsrecht: Crown Copyright © 2014. Published by Elsevier Ireland Ltd. All rights reserved. Cell Membrane - chemistry Dimethylpolysiloxanes - chemistry Lipid Bilayers - chemistry Cristofolini, Luigi oth Cicuta, Pietro oth Enthalten in Chemistry and physics of lipids Shannon [u.a.] : Elsevier Scientific Publishers, 1966 185(2015), Seite 78-87 (DE-627)129526878 (DE-600)213869-4 (DE-576)014946831 0009-3084 nnns volume:185 year:2015 pages:78-87 http://dx.doi.org/10.1016/j.chemphyslip.2014.07.012 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25109251 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 AR 185 2015 78-87 |
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10.1016/j.chemphyslip.2014.07.012 doi PQ20160617 (DE-627)OLC1966944675 (DE-599)GBVOLC1966944675 (PRQ)c1336-787702bcd39c22c15b0cbab19b3be39dacfdec71f467c3c764731e0472aacc9d0 (KEY)0065792120150000185000000078softpinningofliquiddomainsontopographicalhemispher DE-627 ger DE-627 rakwb eng 570 540 530 DNB Feriani, Luigi verfasserin aut Soft pinning of liquid domains on topographical hemispherical caps 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances. Nutzungsrecht: Crown Copyright © 2014. Published by Elsevier Ireland Ltd. All rights reserved. Cell Membrane - chemistry Dimethylpolysiloxanes - chemistry Lipid Bilayers - chemistry Cristofolini, Luigi oth Cicuta, Pietro oth Enthalten in Chemistry and physics of lipids Shannon [u.a.] : Elsevier Scientific Publishers, 1966 185(2015), Seite 78-87 (DE-627)129526878 (DE-600)213869-4 (DE-576)014946831 0009-3084 nnns volume:185 year:2015 pages:78-87 http://dx.doi.org/10.1016/j.chemphyslip.2014.07.012 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25109251 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 AR 185 2015 78-87 |
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10.1016/j.chemphyslip.2014.07.012 doi PQ20160617 (DE-627)OLC1966944675 (DE-599)GBVOLC1966944675 (PRQ)c1336-787702bcd39c22c15b0cbab19b3be39dacfdec71f467c3c764731e0472aacc9d0 (KEY)0065792120150000185000000078softpinningofliquiddomainsontopographicalhemispher DE-627 ger DE-627 rakwb eng 570 540 530 DNB Feriani, Luigi verfasserin aut Soft pinning of liquid domains on topographical hemispherical caps 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances. Nutzungsrecht: Crown Copyright © 2014. Published by Elsevier Ireland Ltd. All rights reserved. Cell Membrane - chemistry Dimethylpolysiloxanes - chemistry Lipid Bilayers - chemistry Cristofolini, Luigi oth Cicuta, Pietro oth Enthalten in Chemistry and physics of lipids Shannon [u.a.] : Elsevier Scientific Publishers, 1966 185(2015), Seite 78-87 (DE-627)129526878 (DE-600)213869-4 (DE-576)014946831 0009-3084 nnns volume:185 year:2015 pages:78-87 http://dx.doi.org/10.1016/j.chemphyslip.2014.07.012 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25109251 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 AR 185 2015 78-87 |
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10.1016/j.chemphyslip.2014.07.012 doi PQ20160617 (DE-627)OLC1966944675 (DE-599)GBVOLC1966944675 (PRQ)c1336-787702bcd39c22c15b0cbab19b3be39dacfdec71f467c3c764731e0472aacc9d0 (KEY)0065792120150000185000000078softpinningofliquiddomainsontopographicalhemispher DE-627 ger DE-627 rakwb eng 570 540 530 DNB Feriani, Luigi verfasserin aut Soft pinning of liquid domains on topographical hemispherical caps 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances. Nutzungsrecht: Crown Copyright © 2014. Published by Elsevier Ireland Ltd. All rights reserved. Cell Membrane - chemistry Dimethylpolysiloxanes - chemistry Lipid Bilayers - chemistry Cristofolini, Luigi oth Cicuta, Pietro oth Enthalten in Chemistry and physics of lipids Shannon [u.a.] : Elsevier Scientific Publishers, 1966 185(2015), Seite 78-87 (DE-627)129526878 (DE-600)213869-4 (DE-576)014946831 0009-3084 nnns volume:185 year:2015 pages:78-87 http://dx.doi.org/10.1016/j.chemphyslip.2014.07.012 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25109251 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 AR 185 2015 78-87 |
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The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances. |
abstractGer |
The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances. |
abstract_unstemmed |
The role of lipid composition as a regulator or mediator of processes that take place in biological membranes is a very topical question, and important insights can be gained by studying in vitro model lipid mixture systems. A particular question is the coupling of local curvature to the local phases in membranes of mixed composition. Working with an experimental system of giant unilamellar vesicles of ternary composition, the curvature is imposed by approaching the membrane to a topographically (on the micron scale) patterned surface. Performing experiments, we show that domains of the more disordered phase localise preferentially to regions of higher curvature. We characterise and discuss the strength of this "caging" behaviour. In future, the setup we discuss here could prove useful as a platform to localise domains rich in membrane proteins, or to promote the onset of biochemical processes at specific locations. Finally, we note that the methods developed here could have also applications in bio-sensing, as a similar but metal coated topography can sustain plasmonic resonances. |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 |
title_short |
Soft pinning of liquid domains on topographical hemispherical caps |
url |
http://dx.doi.org/10.1016/j.chemphyslip.2014.07.012 http://www.ncbi.nlm.nih.gov/pubmed/25109251 |
remote_bool |
false |
author2 |
Cristofolini, Luigi Cicuta, Pietro |
author2Str |
Cristofolini, Luigi Cicuta, Pietro |
ppnlink |
129526878 |
mediatype_str_mv |
n |
isOA_txt |
false |
hochschulschrift_bool |
false |
author2_role |
oth oth |
doi_str |
10.1016/j.chemphyslip.2014.07.012 |
up_date |
2024-07-03T23:27:03.807Z |
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1803602334903697408 |
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7.401886 |