Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L
This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 2...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Bagheri, Mahsa [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Applied biochemistry and biotechnology / A - New York, NY : Springer, 1994, 175(2015), 5, Seite 2366 |
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| Übergeordnetes Werk: |
volume:175 ; year:2015 ; number:5 ; pages:2366 |
| Links: |
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| DOI / URN: |
10.1007/s12010-014-1435-8 |
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OLC1967121842 |
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| 520 | |a This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. | ||
| 650 | 4 | |a Plant Proteins - metabolism | |
| 650 | 4 | |a Plant Proteins - analysis | |
| 650 | 4 | |a Colchicine - pharmacology | |
| 650 | 4 | |a Cannabis - genetics | |
| 650 | 4 | |a Cannabis - metabolism | |
| 650 | 4 | |a Carbohydrates - analysis | |
| 650 | 4 | |a Cannabis - drug effects | |
| 650 | 4 | |a Flavonoids - analysis | |
| 650 | 4 | |a Cannabis - chemistry | |
| 650 | 4 | |a Flavonoids - metabolism | |
| 650 | 4 | |a Biochemistry | |
| 650 | 4 | |a Polyploidy | |
| 650 | 4 | |a Medical marijuana | |
| 700 | 1 | |a Mansouri, Hakimeh |4 oth | |
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10.1007/s12010-014-1435-8 doi PQ20160617 (DE-627)OLC1967121842 (DE-599)GBVOLC1967121842 (PRQ)c2055-9245448fa1230f2757c9469bd54306c3b2877c5474f156b5f414a11cc165b3fe0 (KEY)0068751020150000175000502366effectofinducedpolyploidyonsomebiochemicalparamete DE-627 ger DE-627 rakwb eng 570 540 660 DNB 42.00 bkl Bagheri, Mahsa verfasserin aut Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. Plant Proteins - metabolism Plant Proteins - analysis Colchicine - pharmacology Cannabis - genetics Cannabis - metabolism Carbohydrates - analysis Cannabis - drug effects Flavonoids - analysis Cannabis - chemistry Flavonoids - metabolism Biochemistry Polyploidy Medical marijuana Mansouri, Hakimeh oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 175(2015), 5, Seite 2366 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:175 year:2015 number:5 pages:2366 http://dx.doi.org/10.1007/s12010-014-1435-8 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25492688 http://search.proquest.com/docview/1659893804 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 175 2015 5 2366 |
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10.1007/s12010-014-1435-8 doi PQ20160617 (DE-627)OLC1967121842 (DE-599)GBVOLC1967121842 (PRQ)c2055-9245448fa1230f2757c9469bd54306c3b2877c5474f156b5f414a11cc165b3fe0 (KEY)0068751020150000175000502366effectofinducedpolyploidyonsomebiochemicalparamete DE-627 ger DE-627 rakwb eng 570 540 660 DNB 42.00 bkl Bagheri, Mahsa verfasserin aut Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. Plant Proteins - metabolism Plant Proteins - analysis Colchicine - pharmacology Cannabis - genetics Cannabis - metabolism Carbohydrates - analysis Cannabis - drug effects Flavonoids - analysis Cannabis - chemistry Flavonoids - metabolism Biochemistry Polyploidy Medical marijuana Mansouri, Hakimeh oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 175(2015), 5, Seite 2366 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:175 year:2015 number:5 pages:2366 http://dx.doi.org/10.1007/s12010-014-1435-8 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25492688 http://search.proquest.com/docview/1659893804 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 175 2015 5 2366 |
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10.1007/s12010-014-1435-8 doi PQ20160617 (DE-627)OLC1967121842 (DE-599)GBVOLC1967121842 (PRQ)c2055-9245448fa1230f2757c9469bd54306c3b2877c5474f156b5f414a11cc165b3fe0 (KEY)0068751020150000175000502366effectofinducedpolyploidyonsomebiochemicalparamete DE-627 ger DE-627 rakwb eng 570 540 660 DNB 42.00 bkl Bagheri, Mahsa verfasserin aut Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. Plant Proteins - metabolism Plant Proteins - analysis Colchicine - pharmacology Cannabis - genetics Cannabis - metabolism Carbohydrates - analysis Cannabis - drug effects Flavonoids - analysis Cannabis - chemistry Flavonoids - metabolism Biochemistry Polyploidy Medical marijuana Mansouri, Hakimeh oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 175(2015), 5, Seite 2366 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:175 year:2015 number:5 pages:2366 http://dx.doi.org/10.1007/s12010-014-1435-8 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25492688 http://search.proquest.com/docview/1659893804 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 175 2015 5 2366 |
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10.1007/s12010-014-1435-8 doi PQ20160617 (DE-627)OLC1967121842 (DE-599)GBVOLC1967121842 (PRQ)c2055-9245448fa1230f2757c9469bd54306c3b2877c5474f156b5f414a11cc165b3fe0 (KEY)0068751020150000175000502366effectofinducedpolyploidyonsomebiochemicalparamete DE-627 ger DE-627 rakwb eng 570 540 660 DNB 42.00 bkl Bagheri, Mahsa verfasserin aut Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. Plant Proteins - metabolism Plant Proteins - analysis Colchicine - pharmacology Cannabis - genetics Cannabis - metabolism Carbohydrates - analysis Cannabis - drug effects Flavonoids - analysis Cannabis - chemistry Flavonoids - metabolism Biochemistry Polyploidy Medical marijuana Mansouri, Hakimeh oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 175(2015), 5, Seite 2366 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:175 year:2015 number:5 pages:2366 http://dx.doi.org/10.1007/s12010-014-1435-8 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25492688 http://search.proquest.com/docview/1659893804 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 175 2015 5 2366 |
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10.1007/s12010-014-1435-8 doi PQ20160617 (DE-627)OLC1967121842 (DE-599)GBVOLC1967121842 (PRQ)c2055-9245448fa1230f2757c9469bd54306c3b2877c5474f156b5f414a11cc165b3fe0 (KEY)0068751020150000175000502366effectofinducedpolyploidyonsomebiochemicalparamete DE-627 ger DE-627 rakwb eng 570 540 660 DNB 42.00 bkl Bagheri, Mahsa verfasserin aut Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. Plant Proteins - metabolism Plant Proteins - analysis Colchicine - pharmacology Cannabis - genetics Cannabis - metabolism Carbohydrates - analysis Cannabis - drug effects Flavonoids - analysis Cannabis - chemistry Flavonoids - metabolism Biochemistry Polyploidy Medical marijuana Mansouri, Hakimeh oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 175(2015), 5, Seite 2366 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:175 year:2015 number:5 pages:2366 http://dx.doi.org/10.1007/s12010-014-1435-8 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25492688 http://search.proquest.com/docview/1659893804 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 175 2015 5 2366 |
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Enthalten in Applied biochemistry and biotechnology / A 175(2015), 5, Seite 2366 volume:175 year:2015 number:5 pages:2366 |
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Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L |
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effect of induced polyploidy on some biochemical parameters in cannabis sativa l |
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Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L |
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This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. |
| abstractGer |
This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. |
| abstract_unstemmed |
This study is aimed at testing the efficiency of colchicine on inducing polyploidy in Cannabis sativa L. and investigation of effects of polyploidy induction on some primary and secondary metabolites. Shoot tips were treated with three different concentrations of colchicine (0, 0.1, 0.2 % w/v) for 24 or 48 h. The biggest proportion of the almost coplanar tetraploids (43.33 %) and mixoploids (13.33 %) was obtained from the 24-h treatment in 0.2 and 0.1 % w/v, respectively. Colchicine with 0.2 % concentration and 48 h duration was more destructive than 24 h. The ploidy levels were screened with flow cytometry. The biochemical analyses showed that reducing sugars, soluble sugars, total protein, and total flavonoids increased significantly in mixoploid plants compared with tetraploid and diploid plants. Tetraploid plants had a higher amount of total proteins, total flavonoids, and starch in comparison with control plants. The results showed that polyploidization could increase the contents of tetrahydrocannabinol in mixoploid plants only, but tetraploid plants had lower amounts of this substance in comparison with diploids. Also, we found such changes in protein concentration in electrophoresis analysis. In overall, our study suggests that tetraploidization could not be useful to produce tetrahydrocannabinol for commercial use, and in this case, mixoploids are more suitable. |
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Effect of induced polyploidy on some biochemical parameters in Cannabis sativa L |
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