Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions
In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular conflue...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chaudhary, Amrita [verfasserIn] |
|---|
| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Rechteinformationen: |
Nutzungsrecht: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. |
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| Schlagwörter: |
Epithelial Cells - drug effects N-Acetylglucosaminyltransferases - genetics Down-Regulation - drug effects Cell Proliferation - drug effects Gene Expression - drug effects |
|---|
| Übergeordnetes Werk: |
Enthalten in: Journal of ethnopharmacology - Shannon : Elsevier Science Ireland, 1979, 166(2015), Seite 211-219 |
|---|---|
| Übergeordnetes Werk: |
volume:166 ; year:2015 ; pages:211-219 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.jep.2015.03.017 |
|---|
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OLC1968015310 |
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| 245 | 1 | 0 | |a Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions |
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| 520 | |a In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). | ||
| 540 | |a Nutzungsrecht: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. | ||
| 650 | 4 | |a Epithelial Cells - drug effects | |
| 650 | 4 | |a beta Catenin - genetics | |
| 650 | 4 | |a Gene Expression - genetics | |
| 650 | 4 | |a N-Acetylglucosaminyltransferases - genetics | |
| 650 | 4 | |a Membrane Proteins - genetics | |
| 650 | 4 | |a Down-Regulation - drug effects | |
| 650 | 4 | |a Cell Survival - genetics | |
| 650 | 4 | |a Cadherins - genetics | |
| 650 | 4 | |a Keratinocytes - drug effects | |
| 650 | 4 | |a Cell Survival - drug effects | |
| 650 | 4 | |a Up-Regulation - drug effects | |
| 650 | 4 | |a Cell Proliferation - drug effects | |
| 650 | 4 | |a Gene Expression - drug effects | |
| 650 | 4 | |a Cell Movement - drug effects | |
| 650 | 4 | |a RNA, Messenger - genetics | |
| 650 | 4 | |a Wound Healing - genetics | |
| 650 | 4 | |a Biological Factors - pharmacology | |
| 650 | 4 | |a Antioxidants - pharmacology | |
| 650 | 4 | |a Wound Healing - drug effects | |
| 700 | 1 | |a Bag, Swarnendu |4 oth | |
| 700 | 1 | |a Mandal, Mousumi |4 oth | |
| 700 | 1 | |a Krishna Karri, Sri Phani |4 oth | |
| 700 | 1 | |a Barui, Ananya |4 oth | |
| 700 | 1 | |a Rajput, Monika |4 oth | |
| 700 | 1 | |a Banerjee, Provas |4 oth | |
| 700 | 1 | |a Sheet, Debdoot |4 oth | |
| 700 | 1 | |a Chatterjee, Jyotirmoy |4 oth | |
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| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/25794801 |
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10.1016/j.jep.2015.03.017 doi PQ20160617 (DE-627)OLC1968015310 (DE-599)GBVOLC1968015310 (PRQ)c1015-a0d7d732d5360a8e440c26b2603bb6d912fcd575ac92822f7fac4dcbbbd0b7690 (KEY)0090648420150000166000000211modulatingprimemolecularexpressionsandinvitrowound DE-627 ger DE-627 rakwb eng 610 390 DNB PHARM fid 44.40 bkl 73.61 bkl 73.00 bkl Chaudhary, Amrita verfasserin aut Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). Nutzungsrecht: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. Epithelial Cells - drug effects beta Catenin - genetics Gene Expression - genetics N-Acetylglucosaminyltransferases - genetics Membrane Proteins - genetics Down-Regulation - drug effects Cell Survival - genetics Cadherins - genetics Keratinocytes - drug effects Cell Survival - drug effects Up-Regulation - drug effects Cell Proliferation - drug effects Gene Expression - drug effects Cell Movement - drug effects RNA, Messenger - genetics Wound Healing - genetics Biological Factors - pharmacology Antioxidants - pharmacology Wound Healing - drug effects Bag, Swarnendu oth Mandal, Mousumi oth Krishna Karri, Sri Phani oth Barui, Ananya oth Rajput, Monika oth Banerjee, Provas oth Sheet, Debdoot oth Chatterjee, Jyotirmoy oth Enthalten in Journal of ethnopharmacology Shannon : Elsevier Science Ireland, 1979 166(2015), Seite 211-219 (DE-627)129330159 (DE-600)134511-4 (DE-576)014584301 0378-8741 nnns volume:166 year:2015 pages:211-219 http://dx.doi.org/10.1016/j.jep.2015.03.017 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25794801 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-PHARM SSG-OLC-PHA SSG-OLC-ETH SSG-OPC-PHA GBV_ILN_20 GBV_ILN_4012 44.40 AVZ 73.61 AVZ 73.00 AVZ AR 166 2015 211-219 |
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10.1016/j.jep.2015.03.017 doi PQ20160617 (DE-627)OLC1968015310 (DE-599)GBVOLC1968015310 (PRQ)c1015-a0d7d732d5360a8e440c26b2603bb6d912fcd575ac92822f7fac4dcbbbd0b7690 (KEY)0090648420150000166000000211modulatingprimemolecularexpressionsandinvitrowound DE-627 ger DE-627 rakwb eng 610 390 DNB PHARM fid 44.40 bkl 73.61 bkl 73.00 bkl Chaudhary, Amrita verfasserin aut Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). Nutzungsrecht: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. Epithelial Cells - drug effects beta Catenin - genetics Gene Expression - genetics N-Acetylglucosaminyltransferases - genetics Membrane Proteins - genetics Down-Regulation - drug effects Cell Survival - genetics Cadherins - genetics Keratinocytes - drug effects Cell Survival - drug effects Up-Regulation - drug effects Cell Proliferation - drug effects Gene Expression - drug effects Cell Movement - drug effects RNA, Messenger - genetics Wound Healing - genetics Biological Factors - pharmacology Antioxidants - pharmacology Wound Healing - drug effects Bag, Swarnendu oth Mandal, Mousumi oth Krishna Karri, Sri Phani oth Barui, Ananya oth Rajput, Monika oth Banerjee, Provas oth Sheet, Debdoot oth Chatterjee, Jyotirmoy oth Enthalten in Journal of ethnopharmacology Shannon : Elsevier Science Ireland, 1979 166(2015), Seite 211-219 (DE-627)129330159 (DE-600)134511-4 (DE-576)014584301 0378-8741 nnns volume:166 year:2015 pages:211-219 http://dx.doi.org/10.1016/j.jep.2015.03.017 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25794801 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-PHARM SSG-OLC-PHA SSG-OLC-ETH SSG-OPC-PHA GBV_ILN_20 GBV_ILN_4012 44.40 AVZ 73.61 AVZ 73.00 AVZ AR 166 2015 211-219 |
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10.1016/j.jep.2015.03.017 doi PQ20160617 (DE-627)OLC1968015310 (DE-599)GBVOLC1968015310 (PRQ)c1015-a0d7d732d5360a8e440c26b2603bb6d912fcd575ac92822f7fac4dcbbbd0b7690 (KEY)0090648420150000166000000211modulatingprimemolecularexpressionsandinvitrowound DE-627 ger DE-627 rakwb eng 610 390 DNB PHARM fid 44.40 bkl 73.61 bkl 73.00 bkl Chaudhary, Amrita verfasserin aut Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). Nutzungsrecht: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. Epithelial Cells - drug effects beta Catenin - genetics Gene Expression - genetics N-Acetylglucosaminyltransferases - genetics Membrane Proteins - genetics Down-Regulation - drug effects Cell Survival - genetics Cadherins - genetics Keratinocytes - drug effects Cell Survival - drug effects Up-Regulation - drug effects Cell Proliferation - drug effects Gene Expression - drug effects Cell Movement - drug effects RNA, Messenger - genetics Wound Healing - genetics Biological Factors - pharmacology Antioxidants - pharmacology Wound Healing - drug effects Bag, Swarnendu oth Mandal, Mousumi oth Krishna Karri, Sri Phani oth Barui, Ananya oth Rajput, Monika oth Banerjee, Provas oth Sheet, Debdoot oth Chatterjee, Jyotirmoy oth Enthalten in Journal of ethnopharmacology Shannon : Elsevier Science Ireland, 1979 166(2015), Seite 211-219 (DE-627)129330159 (DE-600)134511-4 (DE-576)014584301 0378-8741 nnns volume:166 year:2015 pages:211-219 http://dx.doi.org/10.1016/j.jep.2015.03.017 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25794801 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-PHARM SSG-OLC-PHA SSG-OLC-ETH SSG-OPC-PHA GBV_ILN_20 GBV_ILN_4012 44.40 AVZ 73.61 AVZ 73.00 AVZ AR 166 2015 211-219 |
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10.1016/j.jep.2015.03.017 doi PQ20160617 (DE-627)OLC1968015310 (DE-599)GBVOLC1968015310 (PRQ)c1015-a0d7d732d5360a8e440c26b2603bb6d912fcd575ac92822f7fac4dcbbbd0b7690 (KEY)0090648420150000166000000211modulatingprimemolecularexpressionsandinvitrowound DE-627 ger DE-627 rakwb eng 610 390 DNB PHARM fid 44.40 bkl 73.61 bkl 73.00 bkl Chaudhary, Amrita verfasserin aut Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). Nutzungsrecht: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. Epithelial Cells - drug effects beta Catenin - genetics Gene Expression - genetics N-Acetylglucosaminyltransferases - genetics Membrane Proteins - genetics Down-Regulation - drug effects Cell Survival - genetics Cadherins - genetics Keratinocytes - drug effects Cell Survival - drug effects Up-Regulation - drug effects Cell Proliferation - drug effects Gene Expression - drug effects Cell Movement - drug effects RNA, Messenger - genetics Wound Healing - genetics Biological Factors - pharmacology Antioxidants - pharmacology Wound Healing - drug effects Bag, Swarnendu oth Mandal, Mousumi oth Krishna Karri, Sri Phani oth Barui, Ananya oth Rajput, Monika oth Banerjee, Provas oth Sheet, Debdoot oth Chatterjee, Jyotirmoy oth Enthalten in Journal of ethnopharmacology Shannon : Elsevier Science Ireland, 1979 166(2015), Seite 211-219 (DE-627)129330159 (DE-600)134511-4 (DE-576)014584301 0378-8741 nnns volume:166 year:2015 pages:211-219 http://dx.doi.org/10.1016/j.jep.2015.03.017 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25794801 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-PHARM SSG-OLC-PHA SSG-OLC-ETH SSG-OPC-PHA GBV_ILN_20 GBV_ILN_4012 44.40 AVZ 73.61 AVZ 73.00 AVZ AR 166 2015 211-219 |
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10.1016/j.jep.2015.03.017 doi PQ20160617 (DE-627)OLC1968015310 (DE-599)GBVOLC1968015310 (PRQ)c1015-a0d7d732d5360a8e440c26b2603bb6d912fcd575ac92822f7fac4dcbbbd0b7690 (KEY)0090648420150000166000000211modulatingprimemolecularexpressionsandinvitrowound DE-627 ger DE-627 rakwb eng 610 390 DNB PHARM fid 44.40 bkl 73.61 bkl 73.00 bkl Chaudhary, Amrita verfasserin aut Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). Nutzungsrecht: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved. Epithelial Cells - drug effects beta Catenin - genetics Gene Expression - genetics N-Acetylglucosaminyltransferases - genetics Membrane Proteins - genetics Down-Regulation - drug effects Cell Survival - genetics Cadherins - genetics Keratinocytes - drug effects Cell Survival - drug effects Up-Regulation - drug effects Cell Proliferation - drug effects Gene Expression - drug effects Cell Movement - drug effects RNA, Messenger - genetics Wound Healing - genetics Biological Factors - pharmacology Antioxidants - pharmacology Wound Healing - drug effects Bag, Swarnendu oth Mandal, Mousumi oth Krishna Karri, Sri Phani oth Barui, Ananya oth Rajput, Monika oth Banerjee, Provas oth Sheet, Debdoot oth Chatterjee, Jyotirmoy oth Enthalten in Journal of ethnopharmacology Shannon : Elsevier Science Ireland, 1979 166(2015), Seite 211-219 (DE-627)129330159 (DE-600)134511-4 (DE-576)014584301 0378-8741 nnns volume:166 year:2015 pages:211-219 http://dx.doi.org/10.1016/j.jep.2015.03.017 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25794801 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-PHARM SSG-OLC-PHA SSG-OLC-ETH SSG-OPC-PHA GBV_ILN_20 GBV_ILN_4012 44.40 AVZ 73.61 AVZ 73.00 AVZ AR 166 2015 211-219 |
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englisch |
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610 390 DNB PHARM fid 44.40 bkl 73.61 bkl 73.00 bkl Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions Epithelial Cells - drug effects beta Catenin - genetics Gene Expression - genetics N-Acetylglucosaminyltransferases - genetics Membrane Proteins - genetics Down-Regulation - drug effects Cell Survival - genetics Cadherins - genetics Keratinocytes - drug effects Cell Survival - drug effects Up-Regulation - drug effects Cell Proliferation - drug effects Gene Expression - drug effects Cell Movement - drug effects RNA, Messenger - genetics Wound Healing - genetics Biological Factors - pharmacology Antioxidants - pharmacology Wound Healing - drug effects |
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modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (hacat) population under characteristic honey dilutions |
| title_auth |
Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions |
| abstract |
In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). |
| abstractGer |
In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). |
| abstract_unstemmed |
In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V). |
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| title_short |
Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1968015310</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20220221143124.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">160206s2015 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.jep.2015.03.017</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20160617</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1968015310</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1968015310</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)c1015-a0d7d732d5360a8e440c26b2603bb6d912fcd575ac92822f7fac4dcbbbd0b7690</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0090648420150000166000000211modulatingprimemolecularexpressionsandinvitrowound</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="a">390</subfield><subfield code="q">DNB</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">PHARM</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.40</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">73.61</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">73.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Chaudhary, Amrita</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Modulating prime molecular expressions and in vitro wound healing rate in keratinocyte (HaCaT) population under characteristic honey dilutions</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">In traditional medicines honey is known for healing efficacy and vividly used as "Anupan" in Ayurvedic medicines appreciating roles in dilutions. Validating efficacy of physico-chemically characterized honey in dilutions, studies on in vitro wound healing and attainment of cellular confluence epithelial cells including expressions of cardinal genes is crucial. To evaluate effects of characterized honey in varied dilutions on cellular viability, in vitro wound healing and modulation of prime epithelial gene expressions. Six Indian honey-samples from different sources were physico-chemically characterized and optimal one was explored in dilutions (v/v%) through in vitro studies on human epithelial (HaCaT) cells for viability, wound healing and expressions of genes p63, E-cadherin, β-catenin, GnT-III and GnT-V. Studied honey samples (i.e. A-F) depicted range of pH (2-4), water (12.48-23.95), electrical conductivity (2.57-14.34), carbohydrate (68.73-98.65), protein (.316-5.36) and antioxidant potential. Though sample A and F showed physico-chemical proximity, but overall bio-impact of the earlier was better, thus studied in 8-.1% (v/v) dilution range. Four dilutions (.01, .04, .1, .25 v/v%) augmented cellular viability but in vitro wound healing was fastest (p<.05) under .1%. Such efficacy was further documented for p63 up-regulation by immunocytochemistry and mRNA studies. The E-cadherin and β-catenin mRNA-expressions were also up-regulated and their proteins were predominantly cytoplasmic. E-cadherin up-regulation was corroborative with down-regulation and up-regulation of GnT-III and GnT-V respectively. Present study illustrated efficacy of particular honey dilution (.1%) with characteristic free radical scavenging activity in facilitating cell proliferation and attainment of confluence towards faster wound healing and modulation of cardinal epithelial genes (viz. p63, E-cadherin, β-catenin, Gnt-III and V).</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Nutzungsrecht: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Epithelial Cells - drug effects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">beta Catenin - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gene Expression - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">N-Acetylglucosaminyltransferases - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Membrane Proteins - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Down-Regulation - drug effects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Survival - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cadherins - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Keratinocytes - drug effects</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cell Survival - 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