β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments
Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminat...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Gao, Na [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Rechteinformationen: |
Nutzungsrecht: Copyright © 2015 Elsevier Ltd. All rights reserved. |
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| Übergeordnetes Werk: |
Enthalten in: Carbohydrate polymers - Barking, Essex : Applied Science Publ., 1981, 127(2015), Seite 427-437 |
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| Übergeordnetes Werk: |
volume:127 ; year:2015 ; pages:427-437 |
| Links: |
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| DOI / URN: |
10.1016/j.carbpol.2015.04.002 |
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OLC1968862978 |
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| 520 | |a Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. | ||
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| 700 | 1 | |a Lu, Feng |4 oth | |
| 700 | 1 | |a Xiao, Chuang |4 oth | |
| 700 | 1 | |a Yang, Lian |4 oth | |
| 700 | 1 | |a Chen, Jun |4 oth | |
| 700 | 1 | |a Zhou, Kai |4 oth | |
| 700 | 1 | |a Wen, Dandan |4 oth | |
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| 700 | 1 | |a Liu, Guangming |4 oth | |
| 700 | 1 | |a Zhao, Jinhua |4 oth | |
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10.1016/j.carbpol.2015.04.002 doi PQ20160617 (DE-627)OLC1968862978 (DE-599)GBVOLC1968862978 (PRQ)c1293-2562378b93833bd735e7db3145635ea2cf3619600f45c81c4ebdf936d8cadfc40 (KEY)0110347420150000127000000427eliminativedepolymerizationofthefucosylatedchondro DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 49.25 bkl Gao, Na verfasserin aut β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. Nutzungsrecht: Copyright © 2015 Elsevier Ltd. All rights reserved. Lu, Feng oth Xiao, Chuang oth Yang, Lian oth Chen, Jun oth Zhou, Kai oth Wen, Dandan oth Li, Zi oth Wu, Mingyi oth Jiang, Jianmin oth Liu, Guangming oth Zhao, Jinhua oth Enthalten in Carbohydrate polymers Barking, Essex : Applied Science Publ., 1981 127(2015), Seite 427-437 (DE-627)130549746 (DE-600)782631-X (DE-576)481341323 0144-8617 nnns volume:127 year:2015 pages:427-437 http://dx.doi.org/10.1016/j.carbpol.2015.04.002 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25965502 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 58.34 AVZ 49.25 AVZ AR 127 2015 427-437 |
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10.1016/j.carbpol.2015.04.002 doi PQ20160617 (DE-627)OLC1968862978 (DE-599)GBVOLC1968862978 (PRQ)c1293-2562378b93833bd735e7db3145635ea2cf3619600f45c81c4ebdf936d8cadfc40 (KEY)0110347420150000127000000427eliminativedepolymerizationofthefucosylatedchondro DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 49.25 bkl Gao, Na verfasserin aut β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. Nutzungsrecht: Copyright © 2015 Elsevier Ltd. All rights reserved. Lu, Feng oth Xiao, Chuang oth Yang, Lian oth Chen, Jun oth Zhou, Kai oth Wen, Dandan oth Li, Zi oth Wu, Mingyi oth Jiang, Jianmin oth Liu, Guangming oth Zhao, Jinhua oth Enthalten in Carbohydrate polymers Barking, Essex : Applied Science Publ., 1981 127(2015), Seite 427-437 (DE-627)130549746 (DE-600)782631-X (DE-576)481341323 0144-8617 nnns volume:127 year:2015 pages:427-437 http://dx.doi.org/10.1016/j.carbpol.2015.04.002 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25965502 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 58.34 AVZ 49.25 AVZ AR 127 2015 427-437 |
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10.1016/j.carbpol.2015.04.002 doi PQ20160617 (DE-627)OLC1968862978 (DE-599)GBVOLC1968862978 (PRQ)c1293-2562378b93833bd735e7db3145635ea2cf3619600f45c81c4ebdf936d8cadfc40 (KEY)0110347420150000127000000427eliminativedepolymerizationofthefucosylatedchondro DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 49.25 bkl Gao, Na verfasserin aut β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. Nutzungsrecht: Copyright © 2015 Elsevier Ltd. All rights reserved. Lu, Feng oth Xiao, Chuang oth Yang, Lian oth Chen, Jun oth Zhou, Kai oth Wen, Dandan oth Li, Zi oth Wu, Mingyi oth Jiang, Jianmin oth Liu, Guangming oth Zhao, Jinhua oth Enthalten in Carbohydrate polymers Barking, Essex : Applied Science Publ., 1981 127(2015), Seite 427-437 (DE-627)130549746 (DE-600)782631-X (DE-576)481341323 0144-8617 nnns volume:127 year:2015 pages:427-437 http://dx.doi.org/10.1016/j.carbpol.2015.04.002 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25965502 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 58.34 AVZ 49.25 AVZ AR 127 2015 427-437 |
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10.1016/j.carbpol.2015.04.002 doi PQ20160617 (DE-627)OLC1968862978 (DE-599)GBVOLC1968862978 (PRQ)c1293-2562378b93833bd735e7db3145635ea2cf3619600f45c81c4ebdf936d8cadfc40 (KEY)0110347420150000127000000427eliminativedepolymerizationofthefucosylatedchondro DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 49.25 bkl Gao, Na verfasserin aut β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. Nutzungsrecht: Copyright © 2015 Elsevier Ltd. All rights reserved. Lu, Feng oth Xiao, Chuang oth Yang, Lian oth Chen, Jun oth Zhou, Kai oth Wen, Dandan oth Li, Zi oth Wu, Mingyi oth Jiang, Jianmin oth Liu, Guangming oth Zhao, Jinhua oth Enthalten in Carbohydrate polymers Barking, Essex : Applied Science Publ., 1981 127(2015), Seite 427-437 (DE-627)130549746 (DE-600)782631-X (DE-576)481341323 0144-8617 nnns volume:127 year:2015 pages:427-437 http://dx.doi.org/10.1016/j.carbpol.2015.04.002 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25965502 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 58.34 AVZ 49.25 AVZ AR 127 2015 427-437 |
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10.1016/j.carbpol.2015.04.002 doi PQ20160617 (DE-627)OLC1968862978 (DE-599)GBVOLC1968862978 (PRQ)c1293-2562378b93833bd735e7db3145635ea2cf3619600f45c81c4ebdf936d8cadfc40 (KEY)0110347420150000127000000427eliminativedepolymerizationofthefucosylatedchondro DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 49.25 bkl Gao, Na verfasserin aut β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. Nutzungsrecht: Copyright © 2015 Elsevier Ltd. All rights reserved. Lu, Feng oth Xiao, Chuang oth Yang, Lian oth Chen, Jun oth Zhou, Kai oth Wen, Dandan oth Li, Zi oth Wu, Mingyi oth Jiang, Jianmin oth Liu, Guangming oth Zhao, Jinhua oth Enthalten in Carbohydrate polymers Barking, Essex : Applied Science Publ., 1981 127(2015), Seite 427-437 (DE-627)130549746 (DE-600)782631-X (DE-576)481341323 0144-8617 nnns volume:127 year:2015 pages:427-437 http://dx.doi.org/10.1016/j.carbpol.2015.04.002 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25965502 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 58.34 AVZ 49.25 AVZ AR 127 2015 427-437 |
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β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments |
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β-eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments |
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β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments |
| abstract |
Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. |
| abstractGer |
Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. |
| abstract_unstemmed |
Fucosylated chondroitin sulfate (FCS) from sea cucumber with complex structure has potent anticoagulant activity by inhibition of intrinsic tenase; however, it could activate factor XII and platelet. To obtain FCS' fragments with selective inhibition on intrinsic tenase, a method for β-eliminative depolymerization of FCS was developed by treating FCS benzyl esters with alkaline in anhydrous solution. Our results demonstrated that the glycosidic linkages of GalNAc-β1, 4-GlcA were selectively cleaved and distinctive Δ(4,5) unsaturated hexuronic acid was formed at non-reducing end of resulting fragments, while the main structures were essentially stable during depolymerization. By this method, five depolymerized fragments (dFCSs) with various molecular sizes were prepared and their anticoagulant activities and activation activities of factor XII and platelet were compared. Overall, dFCSs with Mw 3.2-8.8 kDa reserved potent anticoagulant activities by inhibition of intrinsic tenase, and activation activities of factor XII or platelet could be diminished or eliminated. |
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β-Eliminative depolymerization of the fucosylated chondroitin sulfate and anticoagulant activities of resulting fragments |
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http://dx.doi.org/10.1016/j.carbpol.2015.04.002 http://www.ncbi.nlm.nih.gov/pubmed/25965502 |
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Lu, Feng Xiao, Chuang Yang, Lian Chen, Jun Zhou, Kai Wen, Dandan Li, Zi Wu, Mingyi Jiang, Jianmin Liu, Guangming Zhao, Jinhua |
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Lu, Feng Xiao, Chuang Yang, Lian Chen, Jun Zhou, Kai Wen, Dandan Li, Zi Wu, Mingyi Jiang, Jianmin Liu, Guangming Zhao, Jinhua |
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