Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon
Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF...
Ausführliche Beschreibung
Autor*in: |
Jiang, Li [verfasserIn] |
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Format: |
Artikel |
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Sprache: |
Englisch |
Erschienen: |
2015 |
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Rechteinformationen: |
Nutzungsrecht: Copyright © 2015. Published by Elsevier Ltd. |
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Schlagwörter: |
Plant Growth Regulators - chemistry Organophosphorus Compounds - pharmacology Organophosphorus Compounds - chemistry |
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Übergeordnetes Werk: |
Enthalten in: Food chemistry - Amsterdam [u.a.] : Elsevier, 1976, 176(2015), Seite 27-39 |
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Übergeordnetes Werk: |
volume:176 ; year:2015 ; pages:27-39 |
Links: |
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DOI / URN: |
10.1016/j.foodchem.2014.11.081 |
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Katalog-ID: |
OLC1969365188 |
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520 | |a Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. | ||
540 | |a Nutzungsrecht: Copyright © 2015. Published by Elsevier Ltd. | ||
650 | 4 | |a Chloroplasts - metabolism | |
650 | 4 | |a Asteraceae - metabolism | |
650 | 4 | |a Plant Growth Regulators - chemistry | |
650 | 4 | |a Plant Extracts - chemistry | |
650 | 4 | |a Asteraceae - drug effects | |
650 | 4 | |a Organophosphorus Compounds - pharmacology | |
650 | 4 | |a Organophosphorus Compounds - chemistry | |
650 | 4 | |a Plant Leaves - chemistry | |
650 | 4 | |a Plant Leaves - metabolism | |
650 | 4 | |a Plant Proteins - metabolism | |
650 | 4 | |a Cyclopropanes - chemistry | |
650 | 4 | |a Plant Proteins - chemistry | |
650 | 4 | |a Plant Growth Regulators - pharmacology | |
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700 | 1 | |a Jiang, Juan |4 oth | |
700 | 1 | |a Zhang, Li |4 oth | |
700 | 1 | |a Kang, Ruoyi |4 oth | |
700 | 1 | |a Yu, Zhifang |4 oth | |
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10.1016/j.foodchem.2014.11.081 doi PQ20160211 (DE-627)OLC1969365188 (DE-599)GBVOLC1969365188 (PRQ)c1306-205ee462cc70b68623ae39d4296845ed3556196180ff01d6b37ece61ee7c7b650 (KEY)0039580320150000176000000027differentialproteinprofilesofpostharvestgynurabico DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 58.00 bkl Jiang, Li verfasserin aut Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. Nutzungsrecht: Copyright © 2015. Published by Elsevier Ltd. Chloroplasts - metabolism Asteraceae - metabolism Plant Growth Regulators - chemistry Plant Extracts - chemistry Asteraceae - drug effects Organophosphorus Compounds - pharmacology Organophosphorus Compounds - chemistry Plant Leaves - chemistry Plant Leaves - metabolism Plant Proteins - metabolism Cyclopropanes - chemistry Plant Proteins - chemistry Plant Growth Regulators - pharmacology Cyclopropanes - pharmacology Asteraceae - chemistry Proteomics - methods Plant Leaves - drug effects Chloroplasts - drug effects Jiang, Juan oth Zhang, Li oth Kang, Ruoyi oth Yu, Zhifang oth Enthalten in Food chemistry Amsterdam [u.a.] : Elsevier, 1976 176(2015), Seite 27-39 (DE-627)129611751 (DE-600)243123-3 (DE-576)015107418 0308-8146 nnns volume:176 year:2015 pages:27-39 http://dx.doi.org/10.1016/j.foodchem.2014.11.081 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25624203 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4219 58.34 AVZ 58.00 AVZ AR 176 2015 27-39 |
spelling |
10.1016/j.foodchem.2014.11.081 doi PQ20160211 (DE-627)OLC1969365188 (DE-599)GBVOLC1969365188 (PRQ)c1306-205ee462cc70b68623ae39d4296845ed3556196180ff01d6b37ece61ee7c7b650 (KEY)0039580320150000176000000027differentialproteinprofilesofpostharvestgynurabico DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 58.00 bkl Jiang, Li verfasserin aut Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. Nutzungsrecht: Copyright © 2015. Published by Elsevier Ltd. Chloroplasts - metabolism Asteraceae - metabolism Plant Growth Regulators - chemistry Plant Extracts - chemistry Asteraceae - drug effects Organophosphorus Compounds - pharmacology Organophosphorus Compounds - chemistry Plant Leaves - chemistry Plant Leaves - metabolism Plant Proteins - metabolism Cyclopropanes - chemistry Plant Proteins - chemistry Plant Growth Regulators - pharmacology Cyclopropanes - pharmacology Asteraceae - chemistry Proteomics - methods Plant Leaves - drug effects Chloroplasts - drug effects Jiang, Juan oth Zhang, Li oth Kang, Ruoyi oth Yu, Zhifang oth Enthalten in Food chemistry Amsterdam [u.a.] : Elsevier, 1976 176(2015), Seite 27-39 (DE-627)129611751 (DE-600)243123-3 (DE-576)015107418 0308-8146 nnns volume:176 year:2015 pages:27-39 http://dx.doi.org/10.1016/j.foodchem.2014.11.081 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25624203 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4219 58.34 AVZ 58.00 AVZ AR 176 2015 27-39 |
allfields_unstemmed |
10.1016/j.foodchem.2014.11.081 doi PQ20160211 (DE-627)OLC1969365188 (DE-599)GBVOLC1969365188 (PRQ)c1306-205ee462cc70b68623ae39d4296845ed3556196180ff01d6b37ece61ee7c7b650 (KEY)0039580320150000176000000027differentialproteinprofilesofpostharvestgynurabico DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 58.00 bkl Jiang, Li verfasserin aut Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. Nutzungsrecht: Copyright © 2015. Published by Elsevier Ltd. Chloroplasts - metabolism Asteraceae - metabolism Plant Growth Regulators - chemistry Plant Extracts - chemistry Asteraceae - drug effects Organophosphorus Compounds - pharmacology Organophosphorus Compounds - chemistry Plant Leaves - chemistry Plant Leaves - metabolism Plant Proteins - metabolism Cyclopropanes - chemistry Plant Proteins - chemistry Plant Growth Regulators - pharmacology Cyclopropanes - pharmacology Asteraceae - chemistry Proteomics - methods Plant Leaves - drug effects Chloroplasts - drug effects Jiang, Juan oth Zhang, Li oth Kang, Ruoyi oth Yu, Zhifang oth Enthalten in Food chemistry Amsterdam [u.a.] : Elsevier, 1976 176(2015), Seite 27-39 (DE-627)129611751 (DE-600)243123-3 (DE-576)015107418 0308-8146 nnns volume:176 year:2015 pages:27-39 http://dx.doi.org/10.1016/j.foodchem.2014.11.081 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25624203 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4219 58.34 AVZ 58.00 AVZ AR 176 2015 27-39 |
allfieldsGer |
10.1016/j.foodchem.2014.11.081 doi PQ20160211 (DE-627)OLC1969365188 (DE-599)GBVOLC1969365188 (PRQ)c1306-205ee462cc70b68623ae39d4296845ed3556196180ff01d6b37ece61ee7c7b650 (KEY)0039580320150000176000000027differentialproteinprofilesofpostharvestgynurabico DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 58.00 bkl Jiang, Li verfasserin aut Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. Nutzungsrecht: Copyright © 2015. Published by Elsevier Ltd. Chloroplasts - metabolism Asteraceae - metabolism Plant Growth Regulators - chemistry Plant Extracts - chemistry Asteraceae - drug effects Organophosphorus Compounds - pharmacology Organophosphorus Compounds - chemistry Plant Leaves - chemistry Plant Leaves - metabolism Plant Proteins - metabolism Cyclopropanes - chemistry Plant Proteins - chemistry Plant Growth Regulators - pharmacology Cyclopropanes - pharmacology Asteraceae - chemistry Proteomics - methods Plant Leaves - drug effects Chloroplasts - drug effects Jiang, Juan oth Zhang, Li oth Kang, Ruoyi oth Yu, Zhifang oth Enthalten in Food chemistry Amsterdam [u.a.] : Elsevier, 1976 176(2015), Seite 27-39 (DE-627)129611751 (DE-600)243123-3 (DE-576)015107418 0308-8146 nnns volume:176 year:2015 pages:27-39 http://dx.doi.org/10.1016/j.foodchem.2014.11.081 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25624203 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4219 58.34 AVZ 58.00 AVZ AR 176 2015 27-39 |
allfieldsSound |
10.1016/j.foodchem.2014.11.081 doi PQ20160211 (DE-627)OLC1969365188 (DE-599)GBVOLC1969365188 (PRQ)c1306-205ee462cc70b68623ae39d4296845ed3556196180ff01d6b37ece61ee7c7b650 (KEY)0039580320150000176000000027differentialproteinprofilesofpostharvestgynurabico DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 58.00 bkl Jiang, Li verfasserin aut Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. Nutzungsrecht: Copyright © 2015. Published by Elsevier Ltd. Chloroplasts - metabolism Asteraceae - metabolism Plant Growth Regulators - chemistry Plant Extracts - chemistry Asteraceae - drug effects Organophosphorus Compounds - pharmacology Organophosphorus Compounds - chemistry Plant Leaves - chemistry Plant Leaves - metabolism Plant Proteins - metabolism Cyclopropanes - chemistry Plant Proteins - chemistry Plant Growth Regulators - pharmacology Cyclopropanes - pharmacology Asteraceae - chemistry Proteomics - methods Plant Leaves - drug effects Chloroplasts - drug effects Jiang, Juan oth Zhang, Li oth Kang, Ruoyi oth Yu, Zhifang oth Enthalten in Food chemistry Amsterdam [u.a.] : Elsevier, 1976 176(2015), Seite 27-39 (DE-627)129611751 (DE-600)243123-3 (DE-576)015107418 0308-8146 nnns volume:176 year:2015 pages:27-39 http://dx.doi.org/10.1016/j.foodchem.2014.11.081 Volltext http://www.ncbi.nlm.nih.gov/pubmed/25624203 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4219 58.34 AVZ 58.00 AVZ AR 176 2015 27-39 |
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Enthalten in Food chemistry 176(2015), Seite 27-39 volume:176 year:2015 pages:27-39 |
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Chloroplasts - metabolism Asteraceae - metabolism Plant Growth Regulators - chemistry Plant Extracts - chemistry Asteraceae - drug effects Organophosphorus Compounds - pharmacology Organophosphorus Compounds - chemistry Plant Leaves - chemistry Plant Leaves - metabolism Plant Proteins - metabolism Cyclopropanes - chemistry Plant Proteins - chemistry Plant Growth Regulators - pharmacology Cyclopropanes - pharmacology Asteraceae - chemistry Proteomics - methods Plant Leaves - drug effects Chloroplasts - drug effects |
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Jiang, Li @@aut@@ Jiang, Juan @@oth@@ Zhang, Li @@oth@@ Kang, Ruoyi @@oth@@ Yu, Zhifang @@oth@@ |
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Jiang, Li ddc 540 bkl 58.34 bkl 58.00 misc Chloroplasts - metabolism misc Asteraceae - metabolism misc Plant Growth Regulators - chemistry misc Plant Extracts - chemistry misc Asteraceae - drug effects misc Organophosphorus Compounds - pharmacology misc Organophosphorus Compounds - chemistry misc Plant Leaves - chemistry misc Plant Leaves - metabolism misc Plant Proteins - metabolism misc Cyclopropanes - chemistry misc Plant Proteins - chemistry misc Plant Growth Regulators - pharmacology misc Cyclopropanes - pharmacology misc Asteraceae - chemistry misc Proteomics - methods misc Plant Leaves - drug effects misc Chloroplasts - drug effects Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon |
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540 660 DNB 58.34 bkl 58.00 bkl Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon Chloroplasts - metabolism Asteraceae - metabolism Plant Growth Regulators - chemistry Plant Extracts - chemistry Asteraceae - drug effects Organophosphorus Compounds - pharmacology Organophosphorus Compounds - chemistry Plant Leaves - chemistry Plant Leaves - metabolism Plant Proteins - metabolism Cyclopropanes - chemistry Plant Proteins - chemistry Plant Growth Regulators - pharmacology Cyclopropanes - pharmacology Asteraceae - chemistry Proteomics - methods Plant Leaves - drug effects Chloroplasts - drug effects |
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ddc 540 bkl 58.34 bkl 58.00 misc Chloroplasts - metabolism misc Asteraceae - metabolism misc Plant Growth Regulators - chemistry misc Plant Extracts - chemistry misc Asteraceae - drug effects misc Organophosphorus Compounds - pharmacology misc Organophosphorus Compounds - chemistry misc Plant Leaves - chemistry misc Plant Leaves - metabolism misc Plant Proteins - metabolism misc Cyclopropanes - chemistry misc Plant Proteins - chemistry misc Plant Growth Regulators - pharmacology misc Cyclopropanes - pharmacology misc Asteraceae - chemistry misc Proteomics - methods misc Plant Leaves - drug effects misc Chloroplasts - drug effects |
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ddc 540 bkl 58.34 bkl 58.00 misc Chloroplasts - metabolism misc Asteraceae - metabolism misc Plant Growth Regulators - chemistry misc Plant Extracts - chemistry misc Asteraceae - drug effects misc Organophosphorus Compounds - pharmacology misc Organophosphorus Compounds - chemistry misc Plant Leaves - chemistry misc Plant Leaves - metabolism misc Plant Proteins - metabolism misc Cyclopropanes - chemistry misc Plant Proteins - chemistry misc Plant Growth Regulators - pharmacology misc Cyclopropanes - pharmacology misc Asteraceae - chemistry misc Proteomics - methods misc Plant Leaves - drug effects misc Chloroplasts - drug effects |
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title |
Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon |
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Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon |
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10.1016/j.foodchem.2014.11.081 |
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differential protein profiles of postharvest gynura bicolor d.c leaf treated by 1-methylcyclopropene and ethephon |
title_auth |
Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon |
abstract |
Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. |
abstractGer |
Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. |
abstract_unstemmed |
Proteins were extracted from G. bicolor that had been treated with 1-methylcyclopropene and ethephon and then stored at room temperature for 1, 3 and 7days. More than 300 protein spots were detected by 2-DE and 38 differentially abundant spots (P<0.05) were excised and analysed by using MALDI-TOF/TOF. Thirty-three proteins were finally confidently identified. According to the Clusters of Orthologous Groups of proteins, the proteins identified were classified into those responsible for metabolism (75.8%), information storage and processing (9.1%) and cellular processes and signaling (12.1%). Compared with ethephon and control treatments, 1-methylcyclopropene specifically increased the abundances of superoxide dismutase, peroxidase, carbonic anhydrase, nucleoside diphosphate kinases, glyceraldehyde 3-phosphate dehydrogenase, RuBisCO and ribulose bisphosphate carboxylase/oxygenase activase. 1-Methylcyclopropene protected leaf chloroplast and cells by enhancing stress response and defense, and delayed senescence by inhibiting substance and energy metabolisms. Therefore, 1-methylcyclopropene allowed better self-defense and delayed senescence of G. bicolor leaf. |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_70 GBV_ILN_4012 GBV_ILN_4219 |
title_short |
Differential protein profiles of postharvest Gynura bicolor D.C leaf treated by 1-methylcyclopropene and ethephon |
url |
http://dx.doi.org/10.1016/j.foodchem.2014.11.081 http://www.ncbi.nlm.nih.gov/pubmed/25624203 |
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Jiang, Juan Zhang, Li Kang, Ruoyi Yu, Zhifang |
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