Visualization of cellulose synthases in Arabidopsis secondary cell walls
Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
S Barkwill [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Rechteinformationen: |
Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. © COPYRIGHT 2015 American Association for the Advancement of Science |
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| Übergeordnetes Werk: |
Enthalten in: Science - Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883, 350(2015), 6257, Seite 198-203 |
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| Übergeordnetes Werk: |
volume:350 ; year:2015 ; number:6257 ; pages:198-203 |
| Links: |
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| DOI / URN: |
10.1126/science.aac7446 |
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| Katalog-ID: |
OLC1969546484 |
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| 520 | |a Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science, this issue p. 198, see also p. 156 Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls. | ||
| 540 | |a Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. | ||
| 540 | |a © COPYRIGHT 2015 American Association for the Advancement of Science | ||
| 650 | 4 | |a Flowers & plants | |
| 650 | 4 | |a Enzymes | |
| 650 | 4 | |a Biosynthesis | |
| 650 | 4 | |a Biodiesel fuels | |
| 650 | 4 | |a Cellulose | |
| 650 | 4 | |a Arabidopsis Proteins - chemistry | |
| 650 | 4 | |a Arabidopsis - growth & development | |
| 650 | 4 | |a Luminescent Proteins - analysis | |
| 650 | 4 | |a Arabidopsis Proteins - metabolism | |
| 650 | 4 | |a Cell Wall - ultrastructure | |
| 650 | 4 | |a Golgi Apparatus - enzymology | |
| 650 | 4 | |a Arabidopsis - enzymology | |
| 650 | 4 | |a Microtubules - enzymology | |
| 650 | 4 | |a Cell Wall - enzymology | |
| 650 | 4 | |a Cellulose - biosynthesis | |
| 650 | 4 | |a Glucosyltransferases - chemistry | |
| 650 | 4 | |a Xylem - enzymology | |
| 650 | 4 | |a Glucosyltransferases - metabolism | |
| 650 | 4 | |a Glucosyltransferases - analysis | |
| 650 | 4 | |a Cellulose - metabolism | |
| 650 | 4 | |a Bacterial Proteins - analysis | |
| 650 | 4 | |a Arabidopsis Proteins - analysis | |
| 650 | 4 | |a Xylem - growth & development | |
| 650 | 4 | |a Arabidopsis | |
| 650 | 4 | |a Physiological aspects | |
| 700 | 0 | |a S D Mansfield |4 oth | |
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| 700 | 0 | |a M J Meents |4 oth | |
| 700 | 0 | |a L M McDonnell |4 oth | |
| 700 | 0 | |a D W Ehrhardt |4 oth | |
| 700 | 0 | |a A Sampathkumar |4 oth | |
| 700 | 0 | |a AL Samuels |4 oth | |
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10.1126/science.aac7446 doi PQ20160211 (DE-627)OLC1969546484 (DE-599)GBVOLC1969546484 (PRQ)c2267-83a0c0a0bd088495797e09a3138d7aaaf70b13f29859169d2deb054950c88da50 (KEY)0063888920150000350625700198visualizationofcellulosesynthasesinarabidopsisseco DE-627 ger DE-627 rakwb eng 500 DNB LING fid S Barkwill verfasserin aut Visualization of cellulose synthases in Arabidopsis secondary cell walls 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science, this issue p. 198, see also p. 156 Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. © COPYRIGHT 2015 American Association for the Advancement of Science Flowers & plants Enzymes Biosynthesis Biodiesel fuels Cellulose Arabidopsis Proteins - chemistry Arabidopsis - growth & development Luminescent Proteins - analysis Arabidopsis Proteins - metabolism Cell Wall - ultrastructure Golgi Apparatus - enzymology Arabidopsis - enzymology Microtubules - enzymology Cell Wall - enzymology Cellulose - biosynthesis Glucosyltransferases - chemistry Xylem - enzymology Glucosyltransferases - metabolism Glucosyltransferases - analysis Cellulose - metabolism Bacterial Proteins - analysis Arabidopsis Proteins - analysis Xylem - growth & development Arabidopsis Physiological aspects S D Mansfield oth T Demura oth H N Cartwright oth Y Watanabe oth M J Meents oth L M McDonnell oth D W Ehrhardt oth A Sampathkumar oth AL Samuels oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 350(2015), 6257, Seite 198-203 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:350 year:2015 number:6257 pages:198-203 http://dx.doi.org/10.1126/science.aac7446 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26450210 http://search.proquest.com/docview/1720483127 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 350 2015 6257 198-203 |
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10.1126/science.aac7446 doi PQ20160211 (DE-627)OLC1969546484 (DE-599)GBVOLC1969546484 (PRQ)c2267-83a0c0a0bd088495797e09a3138d7aaaf70b13f29859169d2deb054950c88da50 (KEY)0063888920150000350625700198visualizationofcellulosesynthasesinarabidopsisseco DE-627 ger DE-627 rakwb eng 500 DNB LING fid S Barkwill verfasserin aut Visualization of cellulose synthases in Arabidopsis secondary cell walls 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science, this issue p. 198, see also p. 156 Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. © COPYRIGHT 2015 American Association for the Advancement of Science Flowers & plants Enzymes Biosynthesis Biodiesel fuels Cellulose Arabidopsis Proteins - chemistry Arabidopsis - growth & development Luminescent Proteins - analysis Arabidopsis Proteins - metabolism Cell Wall - ultrastructure Golgi Apparatus - enzymology Arabidopsis - enzymology Microtubules - enzymology Cell Wall - enzymology Cellulose - biosynthesis Glucosyltransferases - chemistry Xylem - enzymology Glucosyltransferases - metabolism Glucosyltransferases - analysis Cellulose - metabolism Bacterial Proteins - analysis Arabidopsis Proteins - analysis Xylem - growth & development Arabidopsis Physiological aspects S D Mansfield oth T Demura oth H N Cartwright oth Y Watanabe oth M J Meents oth L M McDonnell oth D W Ehrhardt oth A Sampathkumar oth AL Samuels oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 350(2015), 6257, Seite 198-203 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:350 year:2015 number:6257 pages:198-203 http://dx.doi.org/10.1126/science.aac7446 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26450210 http://search.proquest.com/docview/1720483127 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 350 2015 6257 198-203 |
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10.1126/science.aac7446 doi PQ20160211 (DE-627)OLC1969546484 (DE-599)GBVOLC1969546484 (PRQ)c2267-83a0c0a0bd088495797e09a3138d7aaaf70b13f29859169d2deb054950c88da50 (KEY)0063888920150000350625700198visualizationofcellulosesynthasesinarabidopsisseco DE-627 ger DE-627 rakwb eng 500 DNB LING fid S Barkwill verfasserin aut Visualization of cellulose synthases in Arabidopsis secondary cell walls 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science, this issue p. 198, see also p. 156 Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. © COPYRIGHT 2015 American Association for the Advancement of Science Flowers & plants Enzymes Biosynthesis Biodiesel fuels Cellulose Arabidopsis Proteins - chemistry Arabidopsis - growth & development Luminescent Proteins - analysis Arabidopsis Proteins - metabolism Cell Wall - ultrastructure Golgi Apparatus - enzymology Arabidopsis - enzymology Microtubules - enzymology Cell Wall - enzymology Cellulose - biosynthesis Glucosyltransferases - chemistry Xylem - enzymology Glucosyltransferases - metabolism Glucosyltransferases - analysis Cellulose - metabolism Bacterial Proteins - analysis Arabidopsis Proteins - analysis Xylem - growth & development Arabidopsis Physiological aspects S D Mansfield oth T Demura oth H N Cartwright oth Y Watanabe oth M J Meents oth L M McDonnell oth D W Ehrhardt oth A Sampathkumar oth AL Samuels oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 350(2015), 6257, Seite 198-203 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:350 year:2015 number:6257 pages:198-203 http://dx.doi.org/10.1126/science.aac7446 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26450210 http://search.proquest.com/docview/1720483127 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 350 2015 6257 198-203 |
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10.1126/science.aac7446 doi PQ20160211 (DE-627)OLC1969546484 (DE-599)GBVOLC1969546484 (PRQ)c2267-83a0c0a0bd088495797e09a3138d7aaaf70b13f29859169d2deb054950c88da50 (KEY)0063888920150000350625700198visualizationofcellulosesynthasesinarabidopsisseco DE-627 ger DE-627 rakwb eng 500 DNB LING fid S Barkwill verfasserin aut Visualization of cellulose synthases in Arabidopsis secondary cell walls 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science, this issue p. 198, see also p. 156 Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls. Nutzungsrecht: Copyright © 2015, American Association for the Advancement of Science. © COPYRIGHT 2015 American Association for the Advancement of Science Flowers & plants Enzymes Biosynthesis Biodiesel fuels Cellulose Arabidopsis Proteins - chemistry Arabidopsis - growth & development Luminescent Proteins - analysis Arabidopsis Proteins - metabolism Cell Wall - ultrastructure Golgi Apparatus - enzymology Arabidopsis - enzymology Microtubules - enzymology Cell Wall - enzymology Cellulose - biosynthesis Glucosyltransferases - chemistry Xylem - enzymology Glucosyltransferases - metabolism Glucosyltransferases - analysis Cellulose - metabolism Bacterial Proteins - analysis Arabidopsis Proteins - analysis Xylem - growth & development Arabidopsis Physiological aspects S D Mansfield oth T Demura oth H N Cartwright oth Y Watanabe oth M J Meents oth L M McDonnell oth D W Ehrhardt oth A Sampathkumar oth AL Samuels oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 350(2015), 6257, Seite 198-203 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:350 year:2015 number:6257 pages:198-203 http://dx.doi.org/10.1126/science.aac7446 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26450210 http://search.proquest.com/docview/1720483127 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2185 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4324 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 350 2015 6257 198-203 |
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visualization of cellulose synthases in arabidopsis secondary cell walls |
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Visualization of cellulose synthases in Arabidopsis secondary cell walls |
| abstract |
Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science, this issue p. 198, see also p. 156 Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls. |
| abstractGer |
Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science, this issue p. 198, see also p. 156 Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls. |
| abstract_unstemmed |
Plant cell walls provide the cellulose that is integral for wood, cotton fiber, and many biofuels. Cellulose is synthesized outside the cell membrane by cellulose synthase enzymes. Much of the secondary cell wall, responsible for the sturdiness of wood, is formed by xylem cells embedded in the core of the plant. Watanabe et al. leveraged ectopic expression to bring xylem-style cellulose synthase activity to the epidermal surface of the plant (see the Perspective by Schneider and Persson). Combining this improved accessibility with fluorescent tagging showed that secondary cell walls are built faster than primary cell walls, perhaps due to increased velocity and density of cellulose synthase complexes. Science, this issue p. 198, see also p. 156 Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls. |
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Visualization of cellulose synthases in Arabidopsis secondary cell walls |
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