Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood
Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and f...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Miller, Shelly L [verfasserIn] |
|---|
| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2015 |
|---|
| Schlagwörter: |
RNA, Ribosomal, 16S - genetics |
|---|
| Übergeordnetes Werk: |
Enthalten in: Environmental science & technology - Washington, DC : ACS Publ., 1967, 49(2015), 5, Seite 2675 |
|---|---|
| Übergeordnetes Werk: |
volume:49 ; year:2015 ; number:5 ; pages:2675 |
| Links: |
|---|
| Katalog-ID: |
OLC1969628553 |
|---|
| LEADER | 01000caa a2200265 4500 | ||
|---|---|---|---|
| 001 | OLC1969628553 | ||
| 003 | DE-627 | ||
| 005 | 20230714174526.0 | ||
| 007 | tu | ||
| 008 | 160211s2015 xx ||||| 00| ||eng c | ||
| 028 | 5 | 2 | |a PQ20160211 |
| 035 | |a (DE-627)OLC1969628553 | ||
| 035 | |a (DE-599)GBVOLC1969628553 | ||
| 035 | |a (PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0 | ||
| 035 | |a (KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 050 |a 333.7 |q DNB |
| 100 | 1 | |a Miller, Shelly L |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood |
| 264 | 1 | |c 2015 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 520 | |a Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. | ||
| 650 | 4 | |a RNA, Ribosomal, 16S - genetics | |
| 650 | 4 | |a RNA, Fungal - genetics | |
| 650 | 4 | |a RNA, Bacterial - genetics | |
| 650 | 4 | |a Bacteria - growth & development | |
| 650 | 4 | |a Bacteria - genetics | |
| 650 | 4 | |a Fungi - growth & development | |
| 650 | 4 | |a Fungi - genetics | |
| 650 | 4 | |a Environmental impact | |
| 650 | 4 | |a Bacteria | |
| 650 | 4 | |a Houses | |
| 650 | 4 | |a Floods | |
| 650 | 4 | |a Fungi | |
| 700 | 1 | |a Brewer, Tess E |4 oth | |
| 700 | 1 | |a Awerbuch, Jonathan |4 oth | |
| 700 | 1 | |a Morgan, Emily E |4 oth | |
| 700 | 1 | |a Keady, Patricia B |4 oth | |
| 700 | 1 | |a Fierer, Noah |4 oth | |
| 700 | 1 | |a Emerson, Joanne B |4 oth | |
| 700 | 1 | |a Clements, Nicholas |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Environmental science & technology |d Washington, DC : ACS Publ., 1967 |g 49(2015), 5, Seite 2675 |w (DE-627)129852457 |w (DE-600)280653-8 |w (DE-576)01515274X |x 0013-936X |7 nnns |
| 773 | 1 | 8 | |g volume:49 |g year:2015 |g number:5 |g pages:2675 |
| 856 | 4 | 2 | |u http://www.ncbi.nlm.nih.gov/pubmed/25643125 |
| 856 | 4 | 2 | |u http://search.proquest.com/docview/1661371282 |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-UMW | ||
| 912 | |a SSG-OLC-TEC | ||
| 912 | |a SSG-OLC-CHE | ||
| 912 | |a GBV_ILN_23 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_252 | ||
| 912 | |a GBV_ILN_2006 | ||
| 912 | |a GBV_ILN_4323 | ||
| 951 | |a AR | ||
| 952 | |d 49 |j 2015 |e 5 |h 2675 | ||
| author_variant |
s l m sl slm |
|---|---|
| matchkey_str |
article:0013936X:2015----::matofodaaenibreatranfninoeatrh21c |
| hierarchy_sort_str |
2015 |
| publishDate |
2015 |
| allfields |
PQ20160211 (DE-627)OLC1969628553 (DE-599)GBVOLC1969628553 (PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0 (KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho DE-627 ger DE-627 rakwb eng 050 333.7 DNB Miller, Shelly L verfasserin aut Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. RNA, Ribosomal, 16S - genetics RNA, Fungal - genetics RNA, Bacterial - genetics Bacteria - growth & development Bacteria - genetics Fungi - growth & development Fungi - genetics Environmental impact Bacteria Houses Floods Fungi Brewer, Tess E oth Awerbuch, Jonathan oth Morgan, Emily E oth Keady, Patricia B oth Fierer, Noah oth Emerson, Joanne B oth Clements, Nicholas oth Enthalten in Environmental science & technology Washington, DC : ACS Publ., 1967 49(2015), 5, Seite 2675 (DE-627)129852457 (DE-600)280653-8 (DE-576)01515274X 0013-936X nnns volume:49 year:2015 number:5 pages:2675 http://www.ncbi.nlm.nih.gov/pubmed/25643125 http://search.proquest.com/docview/1661371282 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2006 GBV_ILN_4323 AR 49 2015 5 2675 |
| spelling |
PQ20160211 (DE-627)OLC1969628553 (DE-599)GBVOLC1969628553 (PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0 (KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho DE-627 ger DE-627 rakwb eng 050 333.7 DNB Miller, Shelly L verfasserin aut Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. RNA, Ribosomal, 16S - genetics RNA, Fungal - genetics RNA, Bacterial - genetics Bacteria - growth & development Bacteria - genetics Fungi - growth & development Fungi - genetics Environmental impact Bacteria Houses Floods Fungi Brewer, Tess E oth Awerbuch, Jonathan oth Morgan, Emily E oth Keady, Patricia B oth Fierer, Noah oth Emerson, Joanne B oth Clements, Nicholas oth Enthalten in Environmental science & technology Washington, DC : ACS Publ., 1967 49(2015), 5, Seite 2675 (DE-627)129852457 (DE-600)280653-8 (DE-576)01515274X 0013-936X nnns volume:49 year:2015 number:5 pages:2675 http://www.ncbi.nlm.nih.gov/pubmed/25643125 http://search.proquest.com/docview/1661371282 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2006 GBV_ILN_4323 AR 49 2015 5 2675 |
| allfields_unstemmed |
PQ20160211 (DE-627)OLC1969628553 (DE-599)GBVOLC1969628553 (PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0 (KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho DE-627 ger DE-627 rakwb eng 050 333.7 DNB Miller, Shelly L verfasserin aut Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. RNA, Ribosomal, 16S - genetics RNA, Fungal - genetics RNA, Bacterial - genetics Bacteria - growth & development Bacteria - genetics Fungi - growth & development Fungi - genetics Environmental impact Bacteria Houses Floods Fungi Brewer, Tess E oth Awerbuch, Jonathan oth Morgan, Emily E oth Keady, Patricia B oth Fierer, Noah oth Emerson, Joanne B oth Clements, Nicholas oth Enthalten in Environmental science & technology Washington, DC : ACS Publ., 1967 49(2015), 5, Seite 2675 (DE-627)129852457 (DE-600)280653-8 (DE-576)01515274X 0013-936X nnns volume:49 year:2015 number:5 pages:2675 http://www.ncbi.nlm.nih.gov/pubmed/25643125 http://search.proquest.com/docview/1661371282 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2006 GBV_ILN_4323 AR 49 2015 5 2675 |
| allfieldsGer |
PQ20160211 (DE-627)OLC1969628553 (DE-599)GBVOLC1969628553 (PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0 (KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho DE-627 ger DE-627 rakwb eng 050 333.7 DNB Miller, Shelly L verfasserin aut Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. RNA, Ribosomal, 16S - genetics RNA, Fungal - genetics RNA, Bacterial - genetics Bacteria - growth & development Bacteria - genetics Fungi - growth & development Fungi - genetics Environmental impact Bacteria Houses Floods Fungi Brewer, Tess E oth Awerbuch, Jonathan oth Morgan, Emily E oth Keady, Patricia B oth Fierer, Noah oth Emerson, Joanne B oth Clements, Nicholas oth Enthalten in Environmental science & technology Washington, DC : ACS Publ., 1967 49(2015), 5, Seite 2675 (DE-627)129852457 (DE-600)280653-8 (DE-576)01515274X 0013-936X nnns volume:49 year:2015 number:5 pages:2675 http://www.ncbi.nlm.nih.gov/pubmed/25643125 http://search.proquest.com/docview/1661371282 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2006 GBV_ILN_4323 AR 49 2015 5 2675 |
| allfieldsSound |
PQ20160211 (DE-627)OLC1969628553 (DE-599)GBVOLC1969628553 (PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0 (KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho DE-627 ger DE-627 rakwb eng 050 333.7 DNB Miller, Shelly L verfasserin aut Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood 2015 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. RNA, Ribosomal, 16S - genetics RNA, Fungal - genetics RNA, Bacterial - genetics Bacteria - growth & development Bacteria - genetics Fungi - growth & development Fungi - genetics Environmental impact Bacteria Houses Floods Fungi Brewer, Tess E oth Awerbuch, Jonathan oth Morgan, Emily E oth Keady, Patricia B oth Fierer, Noah oth Emerson, Joanne B oth Clements, Nicholas oth Enthalten in Environmental science & technology Washington, DC : ACS Publ., 1967 49(2015), 5, Seite 2675 (DE-627)129852457 (DE-600)280653-8 (DE-576)01515274X 0013-936X nnns volume:49 year:2015 number:5 pages:2675 http://www.ncbi.nlm.nih.gov/pubmed/25643125 http://search.proquest.com/docview/1661371282 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2006 GBV_ILN_4323 AR 49 2015 5 2675 |
| language |
English |
| source |
Enthalten in Environmental science & technology 49(2015), 5, Seite 2675 volume:49 year:2015 number:5 pages:2675 |
| sourceStr |
Enthalten in Environmental science & technology 49(2015), 5, Seite 2675 volume:49 year:2015 number:5 pages:2675 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
RNA, Ribosomal, 16S - genetics RNA, Fungal - genetics RNA, Bacterial - genetics Bacteria - growth & development Bacteria - genetics Fungi - growth & development Fungi - genetics Environmental impact Bacteria Houses Floods Fungi |
| dewey-raw |
050 |
| isfreeaccess_bool |
false |
| container_title |
Environmental science & technology |
| authorswithroles_txt_mv |
Miller, Shelly L @@aut@@ Brewer, Tess E @@oth@@ Awerbuch, Jonathan @@oth@@ Morgan, Emily E @@oth@@ Keady, Patricia B @@oth@@ Fierer, Noah @@oth@@ Emerson, Joanne B @@oth@@ Clements, Nicholas @@oth@@ |
| publishDateDaySort_date |
2015-01-01T00:00:00Z |
| hierarchy_top_id |
129852457 |
| dewey-sort |
250 |
| id |
OLC1969628553 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1969628553</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230714174526.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">160211s2015 xx ||||| 00| ||eng c</controlfield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20160211</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1969628553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1969628553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">050</subfield><subfield code="a">333.7</subfield><subfield code="q">DNB</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Miller, Shelly L</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA, Ribosomal, 16S - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA, Fungal - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA, Bacterial - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacteria - growth & development</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacteria - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fungi - growth & development</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fungi - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Environmental impact</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacteria</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Houses</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Floods</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fungi</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Brewer, Tess E</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Awerbuch, Jonathan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Morgan, Emily E</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Keady, Patricia B</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fierer, Noah</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Emerson, Joanne B</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Clements, Nicholas</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Environmental science & technology</subfield><subfield code="d">Washington, DC : ACS Publ., 1967</subfield><subfield code="g">49(2015), 5, Seite 2675</subfield><subfield code="w">(DE-627)129852457</subfield><subfield code="w">(DE-600)280653-8</subfield><subfield code="w">(DE-576)01515274X</subfield><subfield code="x">0013-936X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:49</subfield><subfield code="g">year:2015</subfield><subfield code="g">number:5</subfield><subfield code="g">pages:2675</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://www.ncbi.nlm.nih.gov/pubmed/25643125</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://search.proquest.com/docview/1661371282</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_252</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">49</subfield><subfield code="j">2015</subfield><subfield code="e">5</subfield><subfield code="h">2675</subfield></datafield></record></collection>
|
| author |
Miller, Shelly L |
| spellingShingle |
Miller, Shelly L ddc 050 misc RNA, Ribosomal, 16S - genetics misc RNA, Fungal - genetics misc RNA, Bacterial - genetics misc Bacteria - growth & development misc Bacteria - genetics misc Fungi - growth & development misc Fungi - genetics misc Environmental impact misc Bacteria misc Houses misc Floods misc Fungi Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood |
| authorStr |
Miller, Shelly L |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)129852457 |
| format |
Article |
| dewey-ones |
050 - General serial publications 333 - Economics of land & energy |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
OLC |
| remote_str |
false |
| illustrated |
Not Illustrated |
| issn |
0013-936X |
| topic_title |
050 333.7 DNB Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood RNA, Ribosomal, 16S - genetics RNA, Fungal - genetics RNA, Bacterial - genetics Bacteria - growth & development Bacteria - genetics Fungi - growth & development Fungi - genetics Environmental impact Bacteria Houses Floods Fungi |
| topic |
ddc 050 misc RNA, Ribosomal, 16S - genetics misc RNA, Fungal - genetics misc RNA, Bacterial - genetics misc Bacteria - growth & development misc Bacteria - genetics misc Fungi - growth & development misc Fungi - genetics misc Environmental impact misc Bacteria misc Houses misc Floods misc Fungi |
| topic_unstemmed |
ddc 050 misc RNA, Ribosomal, 16S - genetics misc RNA, Fungal - genetics misc RNA, Bacterial - genetics misc Bacteria - growth & development misc Bacteria - genetics misc Fungi - growth & development misc Fungi - genetics misc Environmental impact misc Bacteria misc Houses misc Floods misc Fungi |
| topic_browse |
ddc 050 misc RNA, Ribosomal, 16S - genetics misc RNA, Fungal - genetics misc RNA, Bacterial - genetics misc Bacteria - growth & development misc Bacteria - genetics misc Fungi - growth & development misc Fungi - genetics misc Environmental impact misc Bacteria misc Houses misc Floods misc Fungi |
| format_facet |
Aufsätze Gedruckte Aufsätze |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
nc |
| author2_variant |
t e b te teb j a ja e e m ee eem p b k pb pbk n f nf j b e jb jbe n c nc |
| hierarchy_parent_title |
Environmental science & technology |
| hierarchy_parent_id |
129852457 |
| dewey-tens |
050 - Magazines, journals & serials 330 - Economics |
| hierarchy_top_title |
Environmental science & technology |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)129852457 (DE-600)280653-8 (DE-576)01515274X |
| title |
Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood |
| ctrlnum |
(DE-627)OLC1969628553 (DE-599)GBVOLC1969628553 (PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0 (KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho |
| title_full |
Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood |
| author_sort |
Miller, Shelly L |
| journal |
Environmental science & technology |
| journalStr |
Environmental science & technology |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
000 - Computer science, information & general works 300 - Social sciences |
| recordtype |
marc |
| publishDateSort |
2015 |
| contenttype_str_mv |
txt |
| container_start_page |
2675 |
| author_browse |
Miller, Shelly L |
| container_volume |
49 |
| class |
050 333.7 DNB |
| format_se |
Aufsätze |
| author-letter |
Miller, Shelly L |
| dewey-full |
050 333.7 |
| title_sort |
impacts of flood damage on airborne bacteria and fungi in homes after the 2013 colorado front range flood |
| title_auth |
Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood |
| abstract |
Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. |
| abstractGer |
Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. |
| abstract_unstemmed |
Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-UMW SSG-OLC-TEC SSG-OLC-CHE GBV_ILN_23 GBV_ILN_70 GBV_ILN_252 GBV_ILN_2006 GBV_ILN_4323 |
| container_issue |
5 |
| title_short |
Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood |
| url |
http://www.ncbi.nlm.nih.gov/pubmed/25643125 http://search.proquest.com/docview/1661371282 |
| remote_bool |
false |
| author2 |
Brewer, Tess E Awerbuch, Jonathan Morgan, Emily E Keady, Patricia B Fierer, Noah Emerson, Joanne B Clements, Nicholas |
| author2Str |
Brewer, Tess E Awerbuch, Jonathan Morgan, Emily E Keady, Patricia B Fierer, Noah Emerson, Joanne B Clements, Nicholas |
| ppnlink |
129852457 |
| mediatype_str_mv |
n |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth oth oth |
| up_date |
2024-07-04T05:57:18.361Z |
| _version_ |
1803626886831538176 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1969628553</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230714174526.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">160211s2015 xx ||||| 00| ||eng c</controlfield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20160211</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1969628553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1969628553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)p1636-7448a10c80d7b355e5c5626a483e3530528e1d3d53e38ea0b4102dd676f1223d0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0072627320150000049000502675impactsofflooddamageonairbornebacteriaandfungiinho</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">050</subfield><subfield code="a">333.7</subfield><subfield code="q">DNB</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Miller, Shelly L</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Impacts of flood damage on airborne bacteria and fungi in homes after the 2013 Colorado Front Range flood</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Flood-damaged homes typically have elevated microbial loads, and their occupants have an increased incidence of allergies, asthma, and other respiratory ailments, yet the microbial communities in these homes remain under-studied. Using culture-independent approaches, we characterized bacterial and fungal communities in homes in Boulder, CO, USA 2-3 months after the historic September, 2013 flooding event. We collected passive air samples from basements in 50 homes (36 flood-damaged, 14 non-flooded), and we sequenced the bacterial 16S rRNA gene (V4-V5 region) and the fungal ITS1 region from these samples for community analyses. Quantitative PCR was used to estimate the abundances of bacteria and fungi in the passive air samples. Results indicate significant differences in bacterial and fungal community composition between flooded and non-flooded homes. Fungal abundances were estimated to be three times higher in flooded, relative to non-flooded homes, but there were no significant differences in bacterial abundances. Penicillium (fungi) and Pseudomonadaceae and Enterobacteriaceae (bacteria) were among the most abundant taxa in flooded homes. Our results suggest that bacterial and fungal communities continue to be affected by flooding, even after relative humidity has returned to baseline levels and remediation has removed any visible evidence of flood damage.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA, Ribosomal, 16S - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA, Fungal - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">RNA, Bacterial - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacteria - growth & development</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacteria - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fungi - growth & development</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fungi - genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Environmental impact</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacteria</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Houses</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Floods</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fungi</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Brewer, Tess E</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Awerbuch, Jonathan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Morgan, Emily E</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Keady, Patricia B</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fierer, Noah</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Emerson, Joanne B</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Clements, Nicholas</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Environmental science & technology</subfield><subfield code="d">Washington, DC : ACS Publ., 1967</subfield><subfield code="g">49(2015), 5, Seite 2675</subfield><subfield code="w">(DE-627)129852457</subfield><subfield code="w">(DE-600)280653-8</subfield><subfield code="w">(DE-576)01515274X</subfield><subfield code="x">0013-936X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:49</subfield><subfield code="g">year:2015</subfield><subfield code="g">number:5</subfield><subfield code="g">pages:2675</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://www.ncbi.nlm.nih.gov/pubmed/25643125</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://search.proquest.com/docview/1661371282</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-UMW</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_252</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">49</subfield><subfield code="j">2015</subfield><subfield code="e">5</subfield><subfield code="h">2675</subfield></datafield></record></collection>
|
| score |
7.4019012 |