Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes
The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple s...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kevin V Solomon [verfasserIn] |
|---|
| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Rechteinformationen: |
Nutzungsrecht: Copyright © 2016, American Association for the Advancement of Science. © COPYRIGHT 2016 American Association for the Advancement of Science |
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| Schlagwörter: |
Trichoderma - isolation & purification Gastrointestinal Tract - microbiology |
|---|
| Übergeordnetes Werk: |
Enthalten in: Science - Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883, 351(2016), 6278, Seite 1192-1195 |
|---|---|
| Übergeordnetes Werk: |
volume:351 ; year:2016 ; number:6278 ; pages:1192-1195 |
| Links: |
|---|
| DOI / URN: |
10.1126/science.aad1431 |
|---|
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OLC1972821202 |
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| 520 | |a The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. | ||
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| 650 | 4 | |a Enzymes | |
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| 650 | 4 | |a Biomass | |
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| 650 | 4 | |a Xylans - metabolism | |
| 650 | 4 | |a Trichoderma - isolation & purification | |
| 650 | 4 | |a Trichoderma - enzymology | |
| 650 | 4 | |a Gastrointestinal Tract - microbiology | |
| 650 | 4 | |a RNA, Untranslated - genetics | |
| 650 | 4 | |a Biotechnology - methods | |
| 650 | 4 | |a Cellulases - metabolism | |
| 650 | 4 | |a Trichoderma - genetics | |
| 650 | 4 | |a Cellulases - genetics | |
| 650 | 4 | |a Aspergillus - genetics | |
| 650 | 4 | |a Aspergillus - enzymology | |
| 650 | 4 | |a Cellulases - isolation & purification | |
| 650 | 4 | |a Cellulose - metabolism | |
| 650 | 4 | |a Aspergillus - isolation & purification | |
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| 700 | 0 | |a Michael K Theodorou |4 oth | |
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| 700 | 0 | |a Aviv Regev |4 oth | |
| 700 | 0 | |a Dawn A Thompson |4 oth | |
| 700 | 0 | |a Michelle A O'Malley |4 oth | |
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| 856 | 4 | 1 | |u http://dx.doi.org/10.1126/science.aad1431 |3 Volltext |
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10.1126/science.aad1431 doi PQ20160430 (DE-627)OLC1972821202 (DE-599)GBVOLC1972821202 (PRQ)c2397-3a86fec9cb4ca53019c9c147ea73806160ec02886ec11eb0a2f5e4eea2054cf30 (KEY)0063888920160000351627801192earlybranchinggutfungipossessalargecomprehensivear DE-627 ger DE-627 rakwb eng 500 DNB LING fid Kevin V Solomon verfasserin aut Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. Nutzungsrecht: Copyright © 2016, American Association for the Advancement of Science. © COPYRIGHT 2016 American Association for the Advancement of Science Enzymes Biodegradation Biomass Fungi Xylans - metabolism Trichoderma - isolation & purification Trichoderma - enzymology Gastrointestinal Tract - microbiology RNA, Untranslated - genetics Biotechnology - methods Cellulases - metabolism Trichoderma - genetics Cellulases - genetics Aspergillus - genetics Aspergillus - enzymology Cellulases - isolation & purification Cellulose - metabolism Aspergillus - isolation & purification Charles H Haitjema oth John K Henske oth Sean P Gilmore oth Diego Borges-Rivera oth Anna Lipzen oth Heather M Brewer oth Samuel O Purvine oth Aaron T Wright oth Michael K Theodorou oth Igor V Grigoriev oth Aviv Regev oth Dawn A Thompson oth Michelle A O'Malley oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 351(2016), 6278, Seite 1192-1195 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:351 year:2016 number:6278 pages:1192-1195 http://dx.doi.org/10.1126/science.aad1431 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26912365 http://search.proquest.com/docview/1772228358 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 351 2016 6278 1192-1195 |
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10.1126/science.aad1431 doi PQ20160430 (DE-627)OLC1972821202 (DE-599)GBVOLC1972821202 (PRQ)c2397-3a86fec9cb4ca53019c9c147ea73806160ec02886ec11eb0a2f5e4eea2054cf30 (KEY)0063888920160000351627801192earlybranchinggutfungipossessalargecomprehensivear DE-627 ger DE-627 rakwb eng 500 DNB LING fid Kevin V Solomon verfasserin aut Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. Nutzungsrecht: Copyright © 2016, American Association for the Advancement of Science. © COPYRIGHT 2016 American Association for the Advancement of Science Enzymes Biodegradation Biomass Fungi Xylans - metabolism Trichoderma - isolation & purification Trichoderma - enzymology Gastrointestinal Tract - microbiology RNA, Untranslated - genetics Biotechnology - methods Cellulases - metabolism Trichoderma - genetics Cellulases - genetics Aspergillus - genetics Aspergillus - enzymology Cellulases - isolation & purification Cellulose - metabolism Aspergillus - isolation & purification Charles H Haitjema oth John K Henske oth Sean P Gilmore oth Diego Borges-Rivera oth Anna Lipzen oth Heather M Brewer oth Samuel O Purvine oth Aaron T Wright oth Michael K Theodorou oth Igor V Grigoriev oth Aviv Regev oth Dawn A Thompson oth Michelle A O'Malley oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 351(2016), 6278, Seite 1192-1195 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:351 year:2016 number:6278 pages:1192-1195 http://dx.doi.org/10.1126/science.aad1431 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26912365 http://search.proquest.com/docview/1772228358 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 351 2016 6278 1192-1195 |
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10.1126/science.aad1431 doi PQ20160430 (DE-627)OLC1972821202 (DE-599)GBVOLC1972821202 (PRQ)c2397-3a86fec9cb4ca53019c9c147ea73806160ec02886ec11eb0a2f5e4eea2054cf30 (KEY)0063888920160000351627801192earlybranchinggutfungipossessalargecomprehensivear DE-627 ger DE-627 rakwb eng 500 DNB LING fid Kevin V Solomon verfasserin aut Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. Nutzungsrecht: Copyright © 2016, American Association for the Advancement of Science. © COPYRIGHT 2016 American Association for the Advancement of Science Enzymes Biodegradation Biomass Fungi Xylans - metabolism Trichoderma - isolation & purification Trichoderma - enzymology Gastrointestinal Tract - microbiology RNA, Untranslated - genetics Biotechnology - methods Cellulases - metabolism Trichoderma - genetics Cellulases - genetics Aspergillus - genetics Aspergillus - enzymology Cellulases - isolation & purification Cellulose - metabolism Aspergillus - isolation & purification Charles H Haitjema oth John K Henske oth Sean P Gilmore oth Diego Borges-Rivera oth Anna Lipzen oth Heather M Brewer oth Samuel O Purvine oth Aaron T Wright oth Michael K Theodorou oth Igor V Grigoriev oth Aviv Regev oth Dawn A Thompson oth Michelle A O'Malley oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 351(2016), 6278, Seite 1192-1195 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:351 year:2016 number:6278 pages:1192-1195 http://dx.doi.org/10.1126/science.aad1431 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26912365 http://search.proquest.com/docview/1772228358 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 351 2016 6278 1192-1195 |
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10.1126/science.aad1431 doi PQ20160430 (DE-627)OLC1972821202 (DE-599)GBVOLC1972821202 (PRQ)c2397-3a86fec9cb4ca53019c9c147ea73806160ec02886ec11eb0a2f5e4eea2054cf30 (KEY)0063888920160000351627801192earlybranchinggutfungipossessalargecomprehensivear DE-627 ger DE-627 rakwb eng 500 DNB LING fid Kevin V Solomon verfasserin aut Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. Nutzungsrecht: Copyright © 2016, American Association for the Advancement of Science. © COPYRIGHT 2016 American Association for the Advancement of Science Enzymes Biodegradation Biomass Fungi Xylans - metabolism Trichoderma - isolation & purification Trichoderma - enzymology Gastrointestinal Tract - microbiology RNA, Untranslated - genetics Biotechnology - methods Cellulases - metabolism Trichoderma - genetics Cellulases - genetics Aspergillus - genetics Aspergillus - enzymology Cellulases - isolation & purification Cellulose - metabolism Aspergillus - isolation & purification Charles H Haitjema oth John K Henske oth Sean P Gilmore oth Diego Borges-Rivera oth Anna Lipzen oth Heather M Brewer oth Samuel O Purvine oth Aaron T Wright oth Michael K Theodorou oth Igor V Grigoriev oth Aviv Regev oth Dawn A Thompson oth Michelle A O'Malley oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 351(2016), 6278, Seite 1192-1195 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:351 year:2016 number:6278 pages:1192-1195 http://dx.doi.org/10.1126/science.aad1431 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26912365 http://search.proquest.com/docview/1772228358 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 351 2016 6278 1192-1195 |
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10.1126/science.aad1431 doi PQ20160430 (DE-627)OLC1972821202 (DE-599)GBVOLC1972821202 (PRQ)c2397-3a86fec9cb4ca53019c9c147ea73806160ec02886ec11eb0a2f5e4eea2054cf30 (KEY)0063888920160000351627801192earlybranchinggutfungipossessalargecomprehensivear DE-627 ger DE-627 rakwb eng 500 DNB LING fid Kevin V Solomon verfasserin aut Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. Nutzungsrecht: Copyright © 2016, American Association for the Advancement of Science. © COPYRIGHT 2016 American Association for the Advancement of Science Enzymes Biodegradation Biomass Fungi Xylans - metabolism Trichoderma - isolation & purification Trichoderma - enzymology Gastrointestinal Tract - microbiology RNA, Untranslated - genetics Biotechnology - methods Cellulases - metabolism Trichoderma - genetics Cellulases - genetics Aspergillus - genetics Aspergillus - enzymology Cellulases - isolation & purification Cellulose - metabolism Aspergillus - isolation & purification Charles H Haitjema oth John K Henske oth Sean P Gilmore oth Diego Borges-Rivera oth Anna Lipzen oth Heather M Brewer oth Samuel O Purvine oth Aaron T Wright oth Michael K Theodorou oth Igor V Grigoriev oth Aviv Regev oth Dawn A Thompson oth Michelle A O'Malley oth Enthalten in Science Washington, DC : AAAS, American Assoc. for the Advancement of Science, 1883 351(2016), 6278, Seite 1192-1195 (DE-627)12931482X (DE-600)128410-1 (DE-576)014533189 0036-8075 nnns volume:351 year:2016 number:6278 pages:1192-1195 http://dx.doi.org/10.1126/science.aad1431 Volltext http://www.ncbi.nlm.nih.gov/pubmed/26912365 http://search.proquest.com/docview/1772228358 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-SPO SSG-OLC-IBL SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_11 GBV_ILN_20 GBV_ILN_21 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_30 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_47 GBV_ILN_55 GBV_ILN_59 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_92 GBV_ILN_101 GBV_ILN_110 GBV_ILN_120 GBV_ILN_131 GBV_ILN_170 GBV_ILN_171 GBV_ILN_179 GBV_ILN_181 GBV_ILN_211 GBV_ILN_252 GBV_ILN_259 GBV_ILN_290 GBV_ILN_600 GBV_ILN_601 GBV_ILN_647 GBV_ILN_754 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2012 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2116 GBV_ILN_2120 GBV_ILN_2121 GBV_ILN_2173 GBV_ILN_2219 GBV_ILN_2221 GBV_ILN_2279 GBV_ILN_2286 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4036 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4310 GBV_ILN_4314 GBV_ILN_4317 GBV_ILN_4318 GBV_ILN_4320 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4700 AR 351 2016 6278 1192-1195 |
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Kevin V Solomon @@aut@@ Charles H Haitjema @@oth@@ John K Henske @@oth@@ Sean P Gilmore @@oth@@ Diego Borges-Rivera @@oth@@ Anna Lipzen @@oth@@ Heather M Brewer @@oth@@ Samuel O Purvine @@oth@@ Aaron T Wright @@oth@@ Michael K Theodorou @@oth@@ Igor V Grigoriev @@oth@@ Aviv Regev @@oth@@ Dawn A Thompson @@oth@@ Michelle A O'Malley @@oth@@ |
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Kevin V Solomon |
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500 DNB LING fid Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes Enzymes Biodegradation Biomass Fungi Xylans - metabolism Trichoderma - isolation & purification Trichoderma - enzymology Gastrointestinal Tract - microbiology RNA, Untranslated - genetics Biotechnology - methods Cellulases - metabolism Trichoderma - genetics Cellulases - genetics Aspergillus - genetics Aspergillus - enzymology Cellulases - isolation & purification Cellulose - metabolism Aspergillus - isolation & purification |
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early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes |
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Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes |
| abstract |
The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. |
| abstractGer |
The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. |
| abstract_unstemmed |
The recalcitrance of plant biomass remains a formidable bottleneck in the production of biofuels and other chemicals from renewable sources. Enzymes from microbial communities found within ruminants and hindgut fermenters, however, show considerable promise to break down plant material into simple sugars efficiently. Solomon et al. used 'omics-level and biochemical assays to reveal a suite of lignocellulose-degrading enzymes from early-diverging anaerobic fungi isolated from the guts of horses, goats, and sheep. This approach not only reveals the regulation of these pathways but also represents a method to identify enzymes with no known homologs that would be unidentifiable using conventional screening methods. Science, this issue p. 1192 The fungal kingdom is the source of almost all industrial enzymes in use for lignocellulose bioprocessing. We developed a systems-level approach that integrates transcriptomic sequencing, proteomics, phenotype, and biochemical studies of relatively unexplored basal fungi. Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. Additionally, we identified several promising sequence-divergent enzyme candidates for lignocellulosic bioprocessing. |
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Early-branching gut fungi possess a large, comprehensive array of biomass-degrading enzymes |
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http://dx.doi.org/10.1126/science.aad1431 http://www.ncbi.nlm.nih.gov/pubmed/26912365 http://search.proquest.com/docview/1772228358 |
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Charles H Haitjema John K Henske Sean P Gilmore Diego Borges-Rivera Anna Lipzen Heather M Brewer Samuel O Purvine Aaron T Wright Michael K Theodorou Igor V Grigoriev Aviv Regev Dawn A Thompson Michelle A O'Malley |
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Charles H Haitjema John K Henske Sean P Gilmore Diego Borges-Rivera Anna Lipzen Heather M Brewer Samuel O Purvine Aaron T Wright Michael K Theodorou Igor V Grigoriev Aviv Regev Dawn A Thompson Michelle A O'Malley |
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10.1126/science.aad1431 |
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2024-07-04T00:40:34.373Z |
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Anaerobic gut fungi isolated from herbivores produce a large array of biomass-degrading enzymes that synergistically degrade crude, untreated plant biomass and are competitive with optimized commercial preparations from Aspergillus and Trichoderma. Compared to these model platforms, gut fungal enzymes are unbiased in substrate preference due to a wealth of xylan-degrading enzymes. These enzymes are universally catabolite-repressed and are further regulated by a rich landscape of noncoding regulatory RNAs. 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