A method for isolation of soil microbial DNA that is suitable for analysis of microbial cellulase genes
We developed a method for isolation of DNA of cellulolytic microbies from soil, so that DNA sample was free from soil organic matter (SOM). The method consists of three procedures: propagation of cellulolytic microbes, separation of microbial cells from SOM, and DNA extraction using cetyl trimethyl-...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kobayashi, Yoshiki [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Rechteinformationen: |
Nutzungsrecht: © 2016 Taylor & Francis 2016 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Separation science and technology - Philadelphia, Pa. : Taylor & Francis, 1978, 51(2016), 6, Seite 1053 |
|---|---|
| Übergeordnetes Werk: |
volume:51 ; year:2016 ; number:6 ; pages:1053 |
| Links: |
|---|
| DOI / URN: |
10.1080/01496395.2015.1119847 |
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OLC1974655911 |
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| 520 | |a We developed a method for isolation of DNA of cellulolytic microbies from soil, so that DNA sample was free from soil organic matter (SOM). The method consists of three procedures: propagation of cellulolytic microbes, separation of microbial cells from SOM, and DNA extraction using cetyl trimethyl-ammonium bromide (CTAB). Differential centrifugation with Ficoll-PM400 successfully separated the cells from soil particles and SOM. From 1 g of soil, 0.12-0.51 μg of microbial DNA with an average size of 15-20 kbp was isolated. Denaturing Gradient Gel Electrophoresis showed that DNA from a variety of cellulolytic microbes was recovered. | ||
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| 700 | 1 | |a Yokoyama, Kazuhira |4 oth | |
| 700 | 1 | |a Fujii, Katsuhiko |4 oth | |
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10.1080/01496395.2015.1119847 doi PQ20160610 (DE-627)OLC1974655911 (DE-599)GBVOLC1974655911 (PRQ)i807-320d9d029e983b18d4b81b7c0cb343edcdd38c505dd6221c731cb7ca07d6ed0 (KEY)0017461020160000051000601053methodforisolationofsoilmicrobialdnathatissuitable DE-627 ger DE-627 rakwb eng 540 DNB Kobayashi, Yoshiki verfasserin aut A method for isolation of soil microbial DNA that is suitable for analysis of microbial cellulase genes 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier We developed a method for isolation of DNA of cellulolytic microbies from soil, so that DNA sample was free from soil organic matter (SOM). The method consists of three procedures: propagation of cellulolytic microbes, separation of microbial cells from SOM, and DNA extraction using cetyl trimethyl-ammonium bromide (CTAB). Differential centrifugation with Ficoll-PM400 successfully separated the cells from soil particles and SOM. From 1 g of soil, 0.12-0.51 μg of microbial DNA with an average size of 15-20 kbp was isolated. Denaturing Gradient Gel Electrophoresis showed that DNA from a variety of cellulolytic microbes was recovered. Nutzungsrecht: © 2016 Taylor & Francis 2016 soil organic matter Soil DNA differential centrifugation Ficoll Hayashida, Erika oth Yokoyama, Kazuhira oth Fujii, Katsuhiko oth Enthalten in Separation science and technology Philadelphia, Pa. : Taylor & Francis, 1978 51(2016), 6, Seite 1053 (DE-627)12994100X (DE-600)391905-5 (DE-576)015504247 0149-6395 nnns volume:51 year:2016 number:6 pages:1053 http://dx.doi.org/10.1080/01496395.2015.1119847 Volltext http://www.tandfonline.com/doi/abs/10.1080/01496395.2015.1119847 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 AR 51 2016 6 1053 |
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method for isolation of soil microbial dna that is suitable for analysis of microbial cellulase genes |
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A method for isolation of soil microbial DNA that is suitable for analysis of microbial cellulase genes |
| abstract |
We developed a method for isolation of DNA of cellulolytic microbies from soil, so that DNA sample was free from soil organic matter (SOM). The method consists of three procedures: propagation of cellulolytic microbes, separation of microbial cells from SOM, and DNA extraction using cetyl trimethyl-ammonium bromide (CTAB). Differential centrifugation with Ficoll-PM400 successfully separated the cells from soil particles and SOM. From 1 g of soil, 0.12-0.51 μg of microbial DNA with an average size of 15-20 kbp was isolated. Denaturing Gradient Gel Electrophoresis showed that DNA from a variety of cellulolytic microbes was recovered. |
| abstractGer |
We developed a method for isolation of DNA of cellulolytic microbies from soil, so that DNA sample was free from soil organic matter (SOM). The method consists of three procedures: propagation of cellulolytic microbes, separation of microbial cells from SOM, and DNA extraction using cetyl trimethyl-ammonium bromide (CTAB). Differential centrifugation with Ficoll-PM400 successfully separated the cells from soil particles and SOM. From 1 g of soil, 0.12-0.51 μg of microbial DNA with an average size of 15-20 kbp was isolated. Denaturing Gradient Gel Electrophoresis showed that DNA from a variety of cellulolytic microbes was recovered. |
| abstract_unstemmed |
We developed a method for isolation of DNA of cellulolytic microbies from soil, so that DNA sample was free from soil organic matter (SOM). The method consists of three procedures: propagation of cellulolytic microbes, separation of microbial cells from SOM, and DNA extraction using cetyl trimethyl-ammonium bromide (CTAB). Differential centrifugation with Ficoll-PM400 successfully separated the cells from soil particles and SOM. From 1 g of soil, 0.12-0.51 μg of microbial DNA with an average size of 15-20 kbp was isolated. Denaturing Gradient Gel Electrophoresis showed that DNA from a variety of cellulolytic microbes was recovered. |
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| container_issue |
6 |
| title_short |
A method for isolation of soil microbial DNA that is suitable for analysis of microbial cellulase genes |
| url |
http://dx.doi.org/10.1080/01496395.2015.1119847 http://www.tandfonline.com/doi/abs/10.1080/01496395.2015.1119847 |
| remote_bool |
false |
| author2 |
Hayashida, Erika Yokoyama, Kazuhira Fujii, Katsuhiko |
| author2Str |
Hayashida, Erika Yokoyama, Kazuhira Fujii, Katsuhiko |
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| doi_str |
10.1080/01496395.2015.1119847 |
| up_date |
2024-07-04T04:47:59.879Z |
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