Tissue-specific DNA demethylation is required for proper B-cell differentiation and function

There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Shari Orlanski [verfasserIn] Verena Labi Yitzhak Reizel Adam Spiro Michal Lichtenstein Rena Levin-Klein Sergei B. Koralov Yael Skversky Klaus Rajewsky Howard Cedar Yehudit Bergman

Format:	Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Deoxyribonucleic acid--DNA Tissue Gene expression Enzymes Genes Cells

Übergeordnetes Werk:	Enthalten in: Proceedings of the National Academy of Sciences of the United States of America - Washington, DC : NAS, 1877, 113(2016), 18, Seite 5018-5023
Übergeordnetes Werk:	volume:113 ; year:2016 ; number:18 ; pages:5018-5023

Links:	Volltext Link aufrufen Link aufrufen Link aufrufen

DOI / URN:	10.1073/pnas.1604365113

Katalog-ID:	OLC1976247632

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allfields	10.1073/pnas.1604365113 doi PQ20160610 (DE-627)OLC1976247632 (DE-599)GBVOLC1976247632 (PRQ)c1156-105d7cbef13b0f7b09ac4ffe213a72ad6c9cb41bc6515f6f4e598b0402672d830 (KEY)0583363920160000113001805018tissuespecificdnademethylationisrequiredforproperb DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Shari Orlanski verfasserin aut Tissue-specific DNA demethylation is required for proper B-cell differentiation and function 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo. Deoxyribonucleic acid--DNA Tissue Gene expression Enzymes Genes Cells Verena Labi oth Yitzhak Reizel oth Adam Spiro oth Michal Lichtenstein oth Rena Levin-Klein oth Sergei B. Koralov oth Yael Skversky oth Klaus Rajewsky oth Howard Cedar oth Yehudit Bergman oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 18, Seite 5018-5023 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:18 pages:5018-5023 http://dx.doi.org/10.1073/pnas.1604365113 Volltext http://www.pnas.org/content/113/18/5018.abstract http://www.ncbi.nlm.nih.gov/pubmed/27091986 http://search.proquest.com/docview/1791897505 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 18 5018-5023
spelling	10.1073/pnas.1604365113 doi PQ20160610 (DE-627)OLC1976247632 (DE-599)GBVOLC1976247632 (PRQ)c1156-105d7cbef13b0f7b09ac4ffe213a72ad6c9cb41bc6515f6f4e598b0402672d830 (KEY)0583363920160000113001805018tissuespecificdnademethylationisrequiredforproperb DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Shari Orlanski verfasserin aut Tissue-specific DNA demethylation is required for proper B-cell differentiation and function 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo. Deoxyribonucleic acid--DNA Tissue Gene expression Enzymes Genes Cells Verena Labi oth Yitzhak Reizel oth Adam Spiro oth Michal Lichtenstein oth Rena Levin-Klein oth Sergei B. Koralov oth Yael Skversky oth Klaus Rajewsky oth Howard Cedar oth Yehudit Bergman oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 18, Seite 5018-5023 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:18 pages:5018-5023 http://dx.doi.org/10.1073/pnas.1604365113 Volltext http://www.pnas.org/content/113/18/5018.abstract http://www.ncbi.nlm.nih.gov/pubmed/27091986 http://search.proquest.com/docview/1791897505 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 18 5018-5023
allfields_unstemmed	10.1073/pnas.1604365113 doi PQ20160610 (DE-627)OLC1976247632 (DE-599)GBVOLC1976247632 (PRQ)c1156-105d7cbef13b0f7b09ac4ffe213a72ad6c9cb41bc6515f6f4e598b0402672d830 (KEY)0583363920160000113001805018tissuespecificdnademethylationisrequiredforproperb DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Shari Orlanski verfasserin aut Tissue-specific DNA demethylation is required for proper B-cell differentiation and function 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo. Deoxyribonucleic acid--DNA Tissue Gene expression Enzymes Genes Cells Verena Labi oth Yitzhak Reizel oth Adam Spiro oth Michal Lichtenstein oth Rena Levin-Klein oth Sergei B. Koralov oth Yael Skversky oth Klaus Rajewsky oth Howard Cedar oth Yehudit Bergman oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 18, Seite 5018-5023 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:18 pages:5018-5023 http://dx.doi.org/10.1073/pnas.1604365113 Volltext http://www.pnas.org/content/113/18/5018.abstract http://www.ncbi.nlm.nih.gov/pubmed/27091986 http://search.proquest.com/docview/1791897505 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 18 5018-5023
allfieldsGer	10.1073/pnas.1604365113 doi PQ20160610 (DE-627)OLC1976247632 (DE-599)GBVOLC1976247632 (PRQ)c1156-105d7cbef13b0f7b09ac4ffe213a72ad6c9cb41bc6515f6f4e598b0402672d830 (KEY)0583363920160000113001805018tissuespecificdnademethylationisrequiredforproperb DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Shari Orlanski verfasserin aut Tissue-specific DNA demethylation is required for proper B-cell differentiation and function 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo. Deoxyribonucleic acid--DNA Tissue Gene expression Enzymes Genes Cells Verena Labi oth Yitzhak Reizel oth Adam Spiro oth Michal Lichtenstein oth Rena Levin-Klein oth Sergei B. Koralov oth Yael Skversky oth Klaus Rajewsky oth Howard Cedar oth Yehudit Bergman oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 18, Seite 5018-5023 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:18 pages:5018-5023 http://dx.doi.org/10.1073/pnas.1604365113 Volltext http://www.pnas.org/content/113/18/5018.abstract http://www.ncbi.nlm.nih.gov/pubmed/27091986 http://search.proquest.com/docview/1791897505 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 18 5018-5023
allfieldsSound	10.1073/pnas.1604365113 doi PQ20160610 (DE-627)OLC1976247632 (DE-599)GBVOLC1976247632 (PRQ)c1156-105d7cbef13b0f7b09ac4ffe213a72ad6c9cb41bc6515f6f4e598b0402672d830 (KEY)0583363920160000113001805018tissuespecificdnademethylationisrequiredforproperb DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Shari Orlanski verfasserin aut Tissue-specific DNA demethylation is required for proper B-cell differentiation and function 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo. Deoxyribonucleic acid--DNA Tissue Gene expression Enzymes Genes Cells Verena Labi oth Yitzhak Reizel oth Adam Spiro oth Michal Lichtenstein oth Rena Levin-Klein oth Sergei B. Koralov oth Yael Skversky oth Klaus Rajewsky oth Howard Cedar oth Yehudit Bergman oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 18, Seite 5018-5023 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:18 pages:5018-5023 http://dx.doi.org/10.1073/pnas.1604365113 Volltext http://www.pnas.org/content/113/18/5018.abstract http://www.ncbi.nlm.nih.gov/pubmed/27091986 http://search.proquest.com/docview/1791897505 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 18 5018-5023
language	English
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author	Shari Orlanski
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topic_title	500 DNB 570 AVZ LING fid BIODIV fid Tissue-specific DNA demethylation is required for proper B-cell differentiation and function Deoxyribonucleic acid--DNA Tissue Gene expression Enzymes Genes Cells
topic	ddc 500 ddc 570 fid LING fid BIODIV misc Deoxyribonucleic acid--DNA misc Tissue misc Gene expression misc Enzymes misc Genes misc Cells
topic_unstemmed	ddc 500 ddc 570 fid LING fid BIODIV misc Deoxyribonucleic acid--DNA misc Tissue misc Gene expression misc Enzymes misc Genes misc Cells
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title	Tissue-specific DNA demethylation is required for proper B-cell differentiation and function
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title_full	Tissue-specific DNA demethylation is required for proper B-cell differentiation and function
author_sort	Shari Orlanski
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title_sort	tissue-specific dna demethylation is required for proper b-cell differentiation and function
title_auth	Tissue-specific DNA demethylation is required for proper B-cell differentiation and function
abstract	There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo.
abstractGer	There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo.
abstract_unstemmed	There is ample evidence that somatic cell differentiation during development is accompanied by extensive DNA demethylation of specific sites that vary between cell types. Although the mechanism of this process has not yet been elucidated, it is likely to involve the conversion of 5mC to 5hmC by Tet enzymes. We show that a Tet2/Tet3 conditional knockout at early stages of B-cell development largely prevents lineage-specific programmed demethylation events. This lack of demethylation affects the expression of nearby B-cell lineage genes by impairing enhancer activity, thus causing defects in B-cell differentiation and function. Thus, tissue-specific DNA demethylation appears to be necessary for proper somatic cell development in vivo.
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container_issue	18
title_short	Tissue-specific DNA demethylation is required for proper B-cell differentiation and function
url	http://dx.doi.org/10.1073/pnas.1604365113 http://www.pnas.org/content/113/18/5018.abstract http://www.ncbi.nlm.nih.gov/pubmed/27091986 http://search.proquest.com/docview/1791897505
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author2	Verena Labi Yitzhak Reizel Adam Spiro Michal Lichtenstein Rena Levin-Klein Sergei B. Koralov Yael Skversky Klaus Rajewsky Howard Cedar Yehudit Bergman
author2Str	Verena Labi Yitzhak Reizel Adam Spiro Michal Lichtenstein Rena Levin-Klein Sergei B. Koralov Yael Skversky Klaus Rajewsky Howard Cedar Yehudit Bergman
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up_date	2024-07-03T15:15:32.895Z
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