Effect of surface modification on the bioactivity of sol–gel TiO2‐based nanomaterials
BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nan...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Alvarez Lemus, Mayra A [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Rechteinformationen: |
Nutzungsrecht: © 2016 Society of Chemical Industry |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of chemical technology and biotechnology - Chichester, Sussex : Wiley, 1979, 91(2016), 8, Seite 2148-2155 |
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| Übergeordnetes Werk: |
volume:91 ; year:2016 ; number:8 ; pages:2148-2155 |
| Links: |
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| DOI / URN: |
10.1002/jctb.4915 |
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OLC1980167931 |
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| 520 | |a BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry | ||
| 540 | |a Nutzungsrecht: © 2016 Society of Chemical Industry | ||
| 650 | 4 | |a aminobutyric acid | |
| 650 | 4 | |a SH‐SY5Y cell line | |
| 650 | 4 | |a xerogel | |
| 650 | 4 | |a titanium dioxide | |
| 650 | 4 | |a neuroblastoma | |
| 650 | 4 | |a platinum (II)acetylacetonate | |
| 700 | 1 | |a Monroy, Hugo |4 oth | |
| 700 | 1 | |a López, Tessy |4 oth | |
| 700 | 1 | |a De la Cruz Hernández, Erick N |4 oth | |
| 700 | 1 | |a López‐González, Rosendo |4 oth | |
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10.1002/jctb.4915 doi PQ20161201 (DE-627)OLC1980167931 (DE-599)GBVOLC1980167931 (PRQ)p1646-a7dfc698a079fcd91d27f62c74c3dd247d12a32192e4c0d18a83638698b2a3b33 (KEY)0074631220160000091000802148effectofsurfacemodificationonthebioactivityofsolge DE-627 ger DE-627 rakwb eng 660 620 DE-600 Alvarez Lemus, Mayra A verfasserin aut Effect of surface modification on the bioactivity of sol–gel TiO2‐based nanomaterials 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry Nutzungsrecht: © 2016 Society of Chemical Industry aminobutyric acid SH‐SY5Y cell line xerogel titanium dioxide neuroblastoma platinum (II)acetylacetonate Monroy, Hugo oth López, Tessy oth De la Cruz Hernández, Erick N oth López‐González, Rosendo oth Enthalten in Journal of chemical technology and biotechnology Chichester, Sussex : Wiley, 1979 91(2016), 8, Seite 2148-2155 (DE-627)12909546X (DE-600)7483-4 (DE-576)014431602 0142-0356 nnns volume:91 year:2016 number:8 pages:2148-2155 http://dx.doi.org/10.1002/jctb.4915 Volltext http://onlinelibrary.wiley.com/doi/10.1002/jctb.4915/abstract http://search.proquest.com/docview/1798787116 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 AR 91 2016 8 2148-2155 |
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10.1002/jctb.4915 doi PQ20161201 (DE-627)OLC1980167931 (DE-599)GBVOLC1980167931 (PRQ)p1646-a7dfc698a079fcd91d27f62c74c3dd247d12a32192e4c0d18a83638698b2a3b33 (KEY)0074631220160000091000802148effectofsurfacemodificationonthebioactivityofsolge DE-627 ger DE-627 rakwb eng 660 620 DE-600 Alvarez Lemus, Mayra A verfasserin aut Effect of surface modification on the bioactivity of sol–gel TiO2‐based nanomaterials 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry Nutzungsrecht: © 2016 Society of Chemical Industry aminobutyric acid SH‐SY5Y cell line xerogel titanium dioxide neuroblastoma platinum (II)acetylacetonate Monroy, Hugo oth López, Tessy oth De la Cruz Hernández, Erick N oth López‐González, Rosendo oth Enthalten in Journal of chemical technology and biotechnology Chichester, Sussex : Wiley, 1979 91(2016), 8, Seite 2148-2155 (DE-627)12909546X (DE-600)7483-4 (DE-576)014431602 0142-0356 nnns volume:91 year:2016 number:8 pages:2148-2155 http://dx.doi.org/10.1002/jctb.4915 Volltext http://onlinelibrary.wiley.com/doi/10.1002/jctb.4915/abstract http://search.proquest.com/docview/1798787116 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 AR 91 2016 8 2148-2155 |
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10.1002/jctb.4915 doi PQ20161201 (DE-627)OLC1980167931 (DE-599)GBVOLC1980167931 (PRQ)p1646-a7dfc698a079fcd91d27f62c74c3dd247d12a32192e4c0d18a83638698b2a3b33 (KEY)0074631220160000091000802148effectofsurfacemodificationonthebioactivityofsolge DE-627 ger DE-627 rakwb eng 660 620 DE-600 Alvarez Lemus, Mayra A verfasserin aut Effect of surface modification on the bioactivity of sol–gel TiO2‐based nanomaterials 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry Nutzungsrecht: © 2016 Society of Chemical Industry aminobutyric acid SH‐SY5Y cell line xerogel titanium dioxide neuroblastoma platinum (II)acetylacetonate Monroy, Hugo oth López, Tessy oth De la Cruz Hernández, Erick N oth López‐González, Rosendo oth Enthalten in Journal of chemical technology and biotechnology Chichester, Sussex : Wiley, 1979 91(2016), 8, Seite 2148-2155 (DE-627)12909546X (DE-600)7483-4 (DE-576)014431602 0142-0356 nnns volume:91 year:2016 number:8 pages:2148-2155 http://dx.doi.org/10.1002/jctb.4915 Volltext http://onlinelibrary.wiley.com/doi/10.1002/jctb.4915/abstract http://search.proquest.com/docview/1798787116 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 AR 91 2016 8 2148-2155 |
| allfieldsGer |
10.1002/jctb.4915 doi PQ20161201 (DE-627)OLC1980167931 (DE-599)GBVOLC1980167931 (PRQ)p1646-a7dfc698a079fcd91d27f62c74c3dd247d12a32192e4c0d18a83638698b2a3b33 (KEY)0074631220160000091000802148effectofsurfacemodificationonthebioactivityofsolge DE-627 ger DE-627 rakwb eng 660 620 DE-600 Alvarez Lemus, Mayra A verfasserin aut Effect of surface modification on the bioactivity of sol–gel TiO2‐based nanomaterials 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry Nutzungsrecht: © 2016 Society of Chemical Industry aminobutyric acid SH‐SY5Y cell line xerogel titanium dioxide neuroblastoma platinum (II)acetylacetonate Monroy, Hugo oth López, Tessy oth De la Cruz Hernández, Erick N oth López‐González, Rosendo oth Enthalten in Journal of chemical technology and biotechnology Chichester, Sussex : Wiley, 1979 91(2016), 8, Seite 2148-2155 (DE-627)12909546X (DE-600)7483-4 (DE-576)014431602 0142-0356 nnns volume:91 year:2016 number:8 pages:2148-2155 http://dx.doi.org/10.1002/jctb.4915 Volltext http://onlinelibrary.wiley.com/doi/10.1002/jctb.4915/abstract http://search.proquest.com/docview/1798787116 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 AR 91 2016 8 2148-2155 |
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10.1002/jctb.4915 doi PQ20161201 (DE-627)OLC1980167931 (DE-599)GBVOLC1980167931 (PRQ)p1646-a7dfc698a079fcd91d27f62c74c3dd247d12a32192e4c0d18a83638698b2a3b33 (KEY)0074631220160000091000802148effectofsurfacemodificationonthebioactivityofsolge DE-627 ger DE-627 rakwb eng 660 620 DE-600 Alvarez Lemus, Mayra A verfasserin aut Effect of surface modification on the bioactivity of sol–gel TiO2‐based nanomaterials 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry Nutzungsrecht: © 2016 Society of Chemical Industry aminobutyric acid SH‐SY5Y cell line xerogel titanium dioxide neuroblastoma platinum (II)acetylacetonate Monroy, Hugo oth López, Tessy oth De la Cruz Hernández, Erick N oth López‐González, Rosendo oth Enthalten in Journal of chemical technology and biotechnology Chichester, Sussex : Wiley, 1979 91(2016), 8, Seite 2148-2155 (DE-627)12909546X (DE-600)7483-4 (DE-576)014431602 0142-0356 nnns volume:91 year:2016 number:8 pages:2148-2155 http://dx.doi.org/10.1002/jctb.4915 Volltext http://onlinelibrary.wiley.com/doi/10.1002/jctb.4915/abstract http://search.proquest.com/docview/1798787116 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 AR 91 2016 8 2148-2155 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1980167931</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230714204713.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">160816s2016 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1002/jctb.4915</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20161201</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1980167931</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1980167931</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)p1646-a7dfc698a079fcd91d27f62c74c3dd247d12a32192e4c0d18a83638698b2a3b33</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0074631220160000091000802148effectofsurfacemodificationonthebioactivityofsolge</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">660</subfield><subfield code="a">620</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Alvarez Lemus, Mayra A</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of surface modification on the bioactivity of sol–gel TiO2‐based nanomaterials</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. 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effect of surface modification on the bioactivity of sol–gel tio2‐based nanomaterials |
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Effect of surface modification on the bioactivity of sol–gel TiO2‐based nanomaterials |
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BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry |
| abstractGer |
BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry |
| abstract_unstemmed |
BACKGROUND Surface composition of titanium dioxide (TiO2) nanoparticles strongly affects their biocompatibility and cytotoxicity. The appropriated functionalization of TiO2 nanoparticles leads to the improvement of these properties; while increasing biocompatibility allows the safety use of TiO2 nanoparticles, their cytotoxicity can be properly used in cancer therapy. RESULTS Amine functionalization of the sol-gel TiO2 nanoparticles was performed by in situ addition of -Gama- aminobutyric acid (GABA)-, and 1% mol of platinum (II) acetylacetonate. Fluoresceine isothiocyanate (FITC) was attached to the surface of the nanoparticles through amine-groups from GABA on the titanium dioxide surface. Nanoparticles obtained formed aggregates of around 100-300 nm. A strong and steady green-emission from labeled nanomaterials was observed. Transmission electron microscopy (TEM) showed that smaller particles (<100 nm) passed through the cellular membrane as they were observed within the cytoplasm and mitochondria. Activation of Caspase-3, a protein involved in apoptosis, was observed in treated cells, which agrees with terminal deoxynucleotidyl transferase dUTP nick end labeling assay results (TUNEL) where the highest DNA fragmentation was observed for Pt-TiO2-GABA nanomaterial. CONCLUSIONS TiO2 amino-functionalized nanoparticles were fluorescently labeled in a simple manner. The nanoparticles formed vesicles and activated a caspase-3 mediated mechanism to induce apoptosis. The addition of acetylacetone together with platinum promoted cell death. © 2016 Society of Chemical Industry |
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Monroy, Hugo López, Tessy De la Cruz Hernández, Erick N López‐González, Rosendo |
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