A new non-degradative method to purify glycogen
Liver glycogen, a complex branched glucose polymer containing a small amount of protein, is important for maintaining glucose homeostasis (blood-sugar control) in humans. It has recently been found that glycogen molecular structure is impaired in diabetes. Isolating the carbohydrate polymer and any...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Tan, Xinle [verfasserIn] |
|---|
| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Rechteinformationen: |
Nutzungsrecht: © Elsevier Ltd Copyright © 2016 Elsevier Ltd. All rights reserved. |
|---|
| Übergeordnetes Werk: |
Enthalten in: Carbohydrate polymers - Barking, Essex : Applied Science Publ., 1981, 147(2016), Seite 165-170 |
|---|---|
| Übergeordnetes Werk: |
volume:147 ; year:2016 ; pages:165-170 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.carbpol.2016.04.009 |
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| 520 | |a Liver glycogen, a complex branched glucose polymer containing a small amount of protein, is important for maintaining glucose homeostasis (blood-sugar control) in humans. It has recently been found that glycogen molecular structure is impaired in diabetes. Isolating the carbohydrate polymer and any intrinsically-attached protein(s) is an essential prerequisite for studying this structural impairment. This requires an effective, non-degradative and efficient purification method to exclude the many other proteins present in liver. Proteins and glycogen have different ranges of molecular sizes. Despite the plethora of proteins that might still be present in significant abundance after other isolation techniques, SEC (size exclusion chromatography, also known as GPC), which separates by molecular size, should separate those extraneous to glycogen from glycogen with any intrinsically associated protein(s). A novel purification method is developed for this, based on preparative SEC following sucrose gradient centrifugation. Proteomics is used to show that the new method compares favourably with current methods in the literature. | ||
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10.1016/j.carbpol.2016.04.009 doi PQ20161201 (DE-627)OLC1980300976 (DE-599)GBVOLC1980300976 (PRQ)c2089-d8387e4123118601b2c13e5ca49c8345781b2aa7ebfd28180942c2bb4062cf490 (KEY)0110347420160000147000000165newnondegradativemethodtopurifyglycogen DE-627 ger DE-627 rakwb eng 540 660 DNB 58.34 bkl 49.25 bkl Tan, Xinle verfasserin aut A new non-degradative method to purify glycogen 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Liver glycogen, a complex branched glucose polymer containing a small amount of protein, is important for maintaining glucose homeostasis (blood-sugar control) in humans. It has recently been found that glycogen molecular structure is impaired in diabetes. Isolating the carbohydrate polymer and any intrinsically-attached protein(s) is an essential prerequisite for studying this structural impairment. This requires an effective, non-degradative and efficient purification method to exclude the many other proteins present in liver. Proteins and glycogen have different ranges of molecular sizes. Despite the plethora of proteins that might still be present in significant abundance after other isolation techniques, SEC (size exclusion chromatography, also known as GPC), which separates by molecular size, should separate those extraneous to glycogen from glycogen with any intrinsically associated protein(s). A novel purification method is developed for this, based on preparative SEC following sucrose gradient centrifugation. Proteomics is used to show that the new method compares favourably with current methods in the literature. Nutzungsrecht: © Elsevier Ltd Copyright © 2016 Elsevier Ltd. All rights reserved. Sullivan, Mitchell A oth Gao, Fei oth Li, Shihan oth Schulz, Benjamin L oth Gilbert, Robert G oth Enthalten in Carbohydrate polymers Barking, Essex : Applied Science Publ., 1981 147(2016), Seite 165-170 (DE-627)130549746 (DE-600)782631-X (DE-576)481341323 0144-8617 nnns volume:147 year:2016 pages:165-170 http://dx.doi.org/10.1016/j.carbpol.2016.04.009 Volltext http://www.sciencedirect.com/science/article/pii/S0144861716303708 http://www.ncbi.nlm.nih.gov/pubmed/27178921 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 58.34 AVZ 49.25 AVZ AR 147 2016 165-170 |
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A new non-degradative method to purify glycogen |
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| title_full |
A new non-degradative method to purify glycogen |
| author_sort |
Tan, Xinle |
| journal |
Carbohydrate polymers |
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Carbohydrate polymers |
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eng |
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500 - Science 600 - Technology |
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2016 |
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165 |
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Tan, Xinle |
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540 660 DNB 58.34 bkl 49.25 bkl |
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Tan, Xinle |
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10.1016/j.carbpol.2016.04.009 |
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540 660 |
| title_sort |
new non-degradative method to purify glycogen |
| title_auth |
A new non-degradative method to purify glycogen |
| abstract |
Liver glycogen, a complex branched glucose polymer containing a small amount of protein, is important for maintaining glucose homeostasis (blood-sugar control) in humans. It has recently been found that glycogen molecular structure is impaired in diabetes. Isolating the carbohydrate polymer and any intrinsically-attached protein(s) is an essential prerequisite for studying this structural impairment. This requires an effective, non-degradative and efficient purification method to exclude the many other proteins present in liver. Proteins and glycogen have different ranges of molecular sizes. Despite the plethora of proteins that might still be present in significant abundance after other isolation techniques, SEC (size exclusion chromatography, also known as GPC), which separates by molecular size, should separate those extraneous to glycogen from glycogen with any intrinsically associated protein(s). A novel purification method is developed for this, based on preparative SEC following sucrose gradient centrifugation. Proteomics is used to show that the new method compares favourably with current methods in the literature. |
| abstractGer |
Liver glycogen, a complex branched glucose polymer containing a small amount of protein, is important for maintaining glucose homeostasis (blood-sugar control) in humans. It has recently been found that glycogen molecular structure is impaired in diabetes. Isolating the carbohydrate polymer and any intrinsically-attached protein(s) is an essential prerequisite for studying this structural impairment. This requires an effective, non-degradative and efficient purification method to exclude the many other proteins present in liver. Proteins and glycogen have different ranges of molecular sizes. Despite the plethora of proteins that might still be present in significant abundance after other isolation techniques, SEC (size exclusion chromatography, also known as GPC), which separates by molecular size, should separate those extraneous to glycogen from glycogen with any intrinsically associated protein(s). A novel purification method is developed for this, based on preparative SEC following sucrose gradient centrifugation. Proteomics is used to show that the new method compares favourably with current methods in the literature. |
| abstract_unstemmed |
Liver glycogen, a complex branched glucose polymer containing a small amount of protein, is important for maintaining glucose homeostasis (blood-sugar control) in humans. It has recently been found that glycogen molecular structure is impaired in diabetes. Isolating the carbohydrate polymer and any intrinsically-attached protein(s) is an essential prerequisite for studying this structural impairment. This requires an effective, non-degradative and efficient purification method to exclude the many other proteins present in liver. Proteins and glycogen have different ranges of molecular sizes. Despite the plethora of proteins that might still be present in significant abundance after other isolation techniques, SEC (size exclusion chromatography, also known as GPC), which separates by molecular size, should separate those extraneous to glycogen from glycogen with any intrinsically associated protein(s). A novel purification method is developed for this, based on preparative SEC following sucrose gradient centrifugation. Proteomics is used to show that the new method compares favourably with current methods in the literature. |
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GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 |
| title_short |
A new non-degradative method to purify glycogen |
| url |
http://dx.doi.org/10.1016/j.carbpol.2016.04.009 http://www.sciencedirect.com/science/article/pii/S0144861716303708 http://www.ncbi.nlm.nih.gov/pubmed/27178921 |
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| author2 |
Sullivan, Mitchell A Gao, Fei Li, Shihan Schulz, Benjamin L Gilbert, Robert G |
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Sullivan, Mitchell A Gao, Fei Li, Shihan Schulz, Benjamin L Gilbert, Robert G |
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10.1016/j.carbpol.2016.04.009 |
| up_date |
2024-07-04T02:49:34.003Z |
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