Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe

A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based g...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Bae, Sena [verfasserIn] Mueller, Olaf Wong, Sandi Rawls, John F Valdivia, Raphael H

Format:	Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Microorganisms Genetics Motility Mutation Digestive system Genomics

Übergeordnetes Werk:	Enthalten in: Proceedings of the National Academy of Sciences of the United States of America - Washington, DC : NAS, 1877, 113(2016), 49, Seite 14127-14132
Übergeordnetes Werk:	volume:113 ; year:2016 ; number:49 ; pages:14127-14132

Links:	Volltext Link aufrufen

DOI / URN:	10.1073/pnas.1612753113

Katalog-ID:	OLC1989956505

Internformat


LEADER	01000caa a2200265 4500
001	OLC1989956505
003	DE-627
005	20230715030734.0
007	tu
008	170207s2016 xx \|\|\|\|\| 00\| \|\|eng c
024	7		\|a 10.1073/pnas.1612753113 \|2 doi
028	5	2	\|a PQ20170301
035			\|a (DE-627)OLC1989956505
035			\|a (DE-599)GBVOLC1989956505
035			\|a (PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60
035			\|a (KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
082	0	4	\|a 500 \|q DNB
082	0	4	\|a 570 \|q AVZ
084			\|a LING \|2 fid
084			\|a BIODIV \|2 fid
100	1		\|a Bae, Sena \|e verfasserin \|4 aut
245	1	0	\|a Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe
264		1	\|c 2016
336			\|a Text \|b txt \|2 rdacontent
337			\|a ohne Hilfsmittel zu benutzen \|b n \|2 rdamedia
338			\|a Band \|b nc \|2 rdacarrier
520			\|a A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools.
650		4	\|a Microorganisms
650		4	\|a Genetics
650		4	\|a Motility
650		4	\|a Mutation
650		4	\|a Digestive system
650		4	\|a Genomics
700	1		\|a Mueller, Olaf \|4 oth
700	1		\|a Wong, Sandi \|4 oth
700	1		\|a Rawls, John F \|4 oth
700	1		\|a Valdivia, Raphael H \|4 oth
773	0	8	\|i Enthalten in \|t Proceedings of the National Academy of Sciences of the United States of America \|d Washington, DC : NAS, 1877 \|g 113(2016), 49, Seite 14127-14132 \|w (DE-627)129505269 \|w (DE-600)209104-5 \|w (DE-576)014909189 \|x 0027-8424 \|7 nnns
773	1	8	\|g volume:113 \|g year:2016 \|g number:49 \|g pages:14127-14132
856	4	1	\|u http://dx.doi.org/10.1073/pnas.1612753113 \|3 Volltext
856	4	2	\|u http://search.proquest.com/docview/1848086873
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_OLC
912			\|a FID-LING
912			\|a FID-BIODIV
912			\|a SSG-OLC-PHY
912			\|a SSG-OLC-CHE
912			\|a SSG-OLC-MAT
912			\|a SSG-OLC-FOR
912			\|a SSG-OLC-PHA
912			\|a SSG-OLC-DE-84
912			\|a SSG-OPC-MAT
912			\|a SSG-OPC-FOR
912			\|a GBV_ILN_40
912			\|a GBV_ILN_59
951			\|a AR
952			\|d 113 \|j 2016 \|e 49 \|h 14127-14132

Indexfelder

author_variant	s b sb
matchkey_str	article:00278424:2016----::eoisqecnbsduainlnihetnlssdniisoiiyeeiaee
hierarchy_sort_str	2016
publishDate	2016
allfields	10.1073/pnas.1612753113 doi PQ20170301 (DE-627)OLC1989956505 (DE-599)GBVOLC1989956505 (PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60 (KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Bae, Sena verfasserin aut Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools. Microorganisms Genetics Motility Mutation Digestive system Genomics Mueller, Olaf oth Wong, Sandi oth Rawls, John F oth Valdivia, Raphael H oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 49, Seite 14127-14132 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:49 pages:14127-14132 http://dx.doi.org/10.1073/pnas.1612753113 Volltext http://search.proquest.com/docview/1848086873 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 49 14127-14132
spelling	10.1073/pnas.1612753113 doi PQ20170301 (DE-627)OLC1989956505 (DE-599)GBVOLC1989956505 (PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60 (KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Bae, Sena verfasserin aut Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools. Microorganisms Genetics Motility Mutation Digestive system Genomics Mueller, Olaf oth Wong, Sandi oth Rawls, John F oth Valdivia, Raphael H oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 49, Seite 14127-14132 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:49 pages:14127-14132 http://dx.doi.org/10.1073/pnas.1612753113 Volltext http://search.proquest.com/docview/1848086873 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 49 14127-14132
allfields_unstemmed	10.1073/pnas.1612753113 doi PQ20170301 (DE-627)OLC1989956505 (DE-599)GBVOLC1989956505 (PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60 (KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Bae, Sena verfasserin aut Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools. Microorganisms Genetics Motility Mutation Digestive system Genomics Mueller, Olaf oth Wong, Sandi oth Rawls, John F oth Valdivia, Raphael H oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 49, Seite 14127-14132 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:49 pages:14127-14132 http://dx.doi.org/10.1073/pnas.1612753113 Volltext http://search.proquest.com/docview/1848086873 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 49 14127-14132
allfieldsGer	10.1073/pnas.1612753113 doi PQ20170301 (DE-627)OLC1989956505 (DE-599)GBVOLC1989956505 (PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60 (KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Bae, Sena verfasserin aut Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools. Microorganisms Genetics Motility Mutation Digestive system Genomics Mueller, Olaf oth Wong, Sandi oth Rawls, John F oth Valdivia, Raphael H oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 49, Seite 14127-14132 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:49 pages:14127-14132 http://dx.doi.org/10.1073/pnas.1612753113 Volltext http://search.proquest.com/docview/1848086873 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 49 14127-14132
allfieldsSound	10.1073/pnas.1612753113 doi PQ20170301 (DE-627)OLC1989956505 (DE-599)GBVOLC1989956505 (PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60 (KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis DE-627 ger DE-627 rakwb eng 500 DNB 570 AVZ LING fid BIODIV fid Bae, Sena verfasserin aut Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools. Microorganisms Genetics Motility Mutation Digestive system Genomics Mueller, Olaf oth Wong, Sandi oth Rawls, John F oth Valdivia, Raphael H oth Enthalten in Proceedings of the National Academy of Sciences of the United States of America Washington, DC : NAS, 1877 113(2016), 49, Seite 14127-14132 (DE-627)129505269 (DE-600)209104-5 (DE-576)014909189 0027-8424 nnns volume:113 year:2016 number:49 pages:14127-14132 http://dx.doi.org/10.1073/pnas.1612753113 Volltext http://search.proquest.com/docview/1848086873 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59 AR 113 2016 49 14127-14132
language	English
source	Enthalten in Proceedings of the National Academy of Sciences of the United States of America 113(2016), 49, Seite 14127-14132 volume:113 year:2016 number:49 pages:14127-14132
sourceStr	Enthalten in Proceedings of the National Academy of Sciences of the United States of America 113(2016), 49, Seite 14127-14132 volume:113 year:2016 number:49 pages:14127-14132
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Microorganisms Genetics Motility Mutation Digestive system Genomics
dewey-raw	500
isfreeaccess_bool	false
container_title	Proceedings of the National Academy of Sciences of the United States of America
authorswithroles_txt_mv	Bae, Sena @@aut@@ Mueller, Olaf @@oth@@ Wong, Sandi @@oth@@ Rawls, John F @@oth@@ Valdivia, Raphael H @@oth@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	129505269
dewey-sort	3500
id	OLC1989956505
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1989956505</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230715030734.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">170207s2016 xx \|\|\|\|\| 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1073/pnas.1612753113</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20170301</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1989956505</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1989956505</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">500</subfield><subfield code="q">DNB</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">AVZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">LING</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="2">fid</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Bae, Sena</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microorganisms</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Motility</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mutation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Digestive system</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genomics</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mueller, Olaf</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wong, Sandi</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rawls, John F</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Valdivia, Raphael H</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Proceedings of the National Academy of Sciences of the United States of America</subfield><subfield code="d">Washington, DC : NAS, 1877</subfield><subfield code="g">113(2016), 49, Seite 14127-14132</subfield><subfield code="w">(DE-627)129505269</subfield><subfield code="w">(DE-600)209104-5</subfield><subfield code="w">(DE-576)014909189</subfield><subfield code="x">0027-8424</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:113</subfield><subfield code="g">year:2016</subfield><subfield code="g">number:49</subfield><subfield code="g">pages:14127-14132</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://dx.doi.org/10.1073/pnas.1612753113</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://search.proquest.com/docview/1848086873</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-LING</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHY</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-MAT</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-FOR</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-MAT</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-FOR</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_59</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">113</subfield><subfield code="j">2016</subfield><subfield code="e">49</subfield><subfield code="h">14127-14132</subfield></datafield></record></collection>
author	Bae, Sena
spellingShingle	Bae, Sena ddc 500 ddc 570 fid LING fid BIODIV misc Microorganisms misc Genetics misc Motility misc Mutation misc Digestive system misc Genomics Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe
authorStr	Bae, Sena
ppnlink_with_tag_str_mv	@@773@@(DE-627)129505269
format	Article
dewey-ones	500 - Natural sciences & mathematics 570 - Life sciences; biology
delete_txt_mv	keep
author_role	aut
collection	OLC
remote_str	false
illustrated	Not Illustrated
issn	0027-8424
topic_title	500 DNB 570 AVZ LING fid BIODIV fid Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe Microorganisms Genetics Motility Mutation Digestive system Genomics
topic	ddc 500 ddc 570 fid LING fid BIODIV misc Microorganisms misc Genetics misc Motility misc Mutation misc Digestive system misc Genomics
topic_unstemmed	ddc 500 ddc 570 fid LING fid BIODIV misc Microorganisms misc Genetics misc Motility misc Mutation misc Digestive system misc Genomics
topic_browse	ddc 500 ddc 570 fid LING fid BIODIV misc Microorganisms misc Genetics misc Motility misc Mutation misc Digestive system misc Genomics
format_facet	Aufsätze Gedruckte Aufsätze
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	nc
author2_variant	o m om s w sw j f r jf jfr r h v rh rhv
hierarchy_parent_title	Proceedings of the National Academy of Sciences of the United States of America
hierarchy_parent_id	129505269
dewey-tens	500 - Science 570 - Life sciences; biology
hierarchy_top_title	Proceedings of the National Academy of Sciences of the United States of America
isfreeaccess_txt	false
familylinks_str_mv	(DE-627)129505269 (DE-600)209104-5 (DE-576)014909189
title	Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe
ctrlnum	(DE-627)OLC1989956505 (DE-599)GBVOLC1989956505 (PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60 (KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis
title_full	Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe
author_sort	Bae, Sena
journal	Proceedings of the National Academy of Sciences of the United States of America
journalStr	Proceedings of the National Academy of Sciences of the United States of America
lang_code	eng
isOA_bool	false
dewey-hundreds	500 - Science
recordtype	marc
publishDateSort	2016
contenttype_str_mv	txt
container_start_page	14127
author_browse	Bae, Sena
container_volume	113
class	500 DNB 570 AVZ LING fid BIODIV fid
format_se	Aufsätze
author-letter	Bae, Sena
doi_str_mv	10.1073/pnas.1612753113
dewey-full	500 570
title_sort	genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe
title_auth	Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe
abstract	A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools.
abstractGer	A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools.
abstract_unstemmed	A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-LING FID-BIODIV SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-MAT SSG-OLC-FOR SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-MAT SSG-OPC-FOR GBV_ILN_40 GBV_ILN_59
container_issue	49
title_short	Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe
url	http://dx.doi.org/10.1073/pnas.1612753113 http://search.proquest.com/docview/1848086873
remote_bool	false
author2	Mueller, Olaf Wong, Sandi Rawls, John F Valdivia, Raphael H
author2Str	Mueller, Olaf Wong, Sandi Rawls, John F Valdivia, Raphael H
ppnlink	129505269
mediatype_str_mv	n
isOA_txt	false
hochschulschrift_bool	false
author2_role	oth oth oth oth
doi_str	10.1073/pnas.1612753113
up_date	2024-07-03T23:21:42.780Z
_version_	1803601998283538432
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1989956505</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230715030734.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">170207s2016 xx \|\|\|\|\| 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1073/pnas.1612753113</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20170301</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1989956505</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1989956505</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)c1573-c87cf57092cbdf10ac9d4d8981046330e02522d2e858a77039c122429ae804c60</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0583363920160000113004914127genomicsequencingbasedmutationalenrichmentanalysis</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">500</subfield><subfield code="q">DNB</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">AVZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">LING</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="2">fid</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Bae, Sena</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">A major roadblock to understanding how microbes in the gastrointestinal tract colonize and influence the physiology of their hosts is our inability to genetically manipulate new bacterial species and experimentally assess the function of their genes. We describe the application of population-based genomic sequencing after chemical mutagenesis to map bacterial genes responsible for motility in Exiguobacterium acetylicum, a representative intestinal Firmicutes bacterium that is intractable to molecular genetic manipulation. We derived strong associations between mutations in 57 E. acetylicum genes and impaired motility. Surprisingly, less than half of these genes were annotated as motility-related based on sequence homologies. We confirmed the genetic link between individual mutations and loss of motility for several of these genes by performing a large-scale analysis of spontaneous suppressor mutations. In the process, we reannotated genes belonging to a broad family of diguanylate cyclases and phosphodiesterases to highlight their specific role in motility and assigned functions to uncharacterized genes. Furthermore, we generated isogenic strains that allowed us to establish that Exiguobacterium motility is important for the colonization of its vertebrate host. These results indicate that genetic dissection of a complex trait, functional annotation of new genes, and the generation of mutant strains to define the role of genes in complex environments can be accomplished in bacteria without the development of species-specific molecular genetic tools.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microorganisms</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Motility</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Mutation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Digestive system</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Genomics</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mueller, Olaf</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wong, Sandi</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Rawls, John F</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Valdivia, Raphael H</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Proceedings of the National Academy of Sciences of the United States of America</subfield><subfield code="d">Washington, DC : NAS, 1877</subfield><subfield code="g">113(2016), 49, Seite 14127-14132</subfield><subfield code="w">(DE-627)129505269</subfield><subfield code="w">(DE-600)209104-5</subfield><subfield code="w">(DE-576)014909189</subfield><subfield code="x">0027-8424</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:113</subfield><subfield code="g">year:2016</subfield><subfield code="g">number:49</subfield><subfield code="g">pages:14127-14132</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://dx.doi.org/10.1073/pnas.1612753113</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://search.proquest.com/docview/1848086873</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-LING</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHY</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-MAT</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-FOR</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-MAT</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OPC-FOR</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_59</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">113</subfield><subfield code="j">2016</subfield><subfield code="e">49</subfield><subfield code="h">14127-14132</subfield></datafield></record></collection>
score	7.3975964

Nicht das Richtige dabei?

Schreiben Sie uns!

Genomic sequencing-based mutational enrichment analysis identifies motility genes in a genetically intractable gut microbe

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?