Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella

AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis rev...
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Autor*in:	Hu, Rong [verfasserIn] Wang, Yonghong Qu, Fufa Tang, Jianzhou Zhou, Yi Lu, Shuangqing Zhang, Jianshe Liu, Zhen Zhou, Zhigang Guo, Xinhong

Format:	Artikel
Sprache:	Englisch

Erschienen:	2017

Rechteinformationen:	Nutzungsrecht: © Copyright by the World Aquaculture Society 2016

Schlagwörter:	Gene expression Proteins Fish Zebrafish

Übergeordnetes Werk:	Enthalten in: Journal of the World Aquaculture Society - Baton Rouge, La. : Soc., 1986, 48(2017), 2, Seite 333-341
Übergeordnetes Werk:	volume:48 ; year:2017 ; number:2 ; pages:333-341

Links:	Volltext Link aufrufen Link aufrufen

DOI / URN:	10.1111/jwas.12339

Katalog-ID:	OLC1991947208

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520			\|a AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives.
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allfields	10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341
spelling	10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341
allfields_unstemmed	10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341
allfieldsGer	10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341
allfieldsSound	10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341
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topic_title	550 ZDB 48.68 bkl Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella Gene expression Proteins Fish Zebrafish
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title	Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella
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title_full	Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella
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title_sort	molecular characterization and dietary regulation of the alasercys transporter 2 in grass carp, ctenopharyngodon idella
title_auth	Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella
abstract	AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives.
abstractGer	AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives.
abstract_unstemmed	AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives.
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title_short	Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella
url	http://dx.doi.org/10.1111/jwas.12339 http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484
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author2	Wang, Yonghong Qu, Fufa Tang, Jianzhou Zhou, Yi Lu, Shuangqing Zhang, Jianshe Liu, Zhen Zhou, Zhigang Guo, Xinhong
author2Str	Wang, Yonghong Qu, Fufa Tang, Jianzhou Zhou, Yi Lu, Shuangqing Zhang, Jianshe Liu, Zhen Zhou, Zhigang Guo, Xinhong
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up_date	2024-07-04T03:56:53.288Z
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