Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella
AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis rev...
Ausführliche Beschreibung
Autor*in: |
Hu, Rong [verfasserIn] |
---|
Format: |
Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2017 |
---|
Rechteinformationen: |
Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 |
---|
Schlagwörter: |
---|
Übergeordnetes Werk: |
Enthalten in: Journal of the World Aquaculture Society - Baton Rouge, La. : Soc., 1986, 48(2017), 2, Seite 333-341 |
---|---|
Übergeordnetes Werk: |
volume:48 ; year:2017 ; number:2 ; pages:333-341 |
Links: |
---|
DOI / URN: |
10.1111/jwas.12339 |
---|
Katalog-ID: |
OLC1991947208 |
---|
LEADER | 01000caa a2200265 4500 | ||
---|---|---|---|
001 | OLC1991947208 | ||
003 | DE-627 | ||
005 | 20220216135840.0 | ||
007 | tu | ||
008 | 170512s2017 xx ||||| 00| ||eng c | ||
024 | 7 | |a 10.1111/jwas.12339 |2 doi | |
028 | 5 | 2 | |a PQ20170901 |
035 | |a (DE-627)OLC1991947208 | ||
035 | |a (DE-599)GBVOLC1991947208 | ||
035 | |a (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 | ||
035 | |a (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 550 |q ZDB |
084 | |a 48.68 |2 bkl | ||
100 | 1 | |a Hu, Rong |e verfasserin |4 aut | |
245 | 1 | 0 | |a Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella |
264 | 1 | |c 2017 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
520 | |a AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. | ||
540 | |a Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 | ||
650 | 4 | |a Gene expression | |
650 | 4 | |a Proteins | |
650 | 4 | |a Fish | |
650 | 4 | |a Zebrafish | |
700 | 1 | |a Wang, Yonghong |4 oth | |
700 | 1 | |a Qu, Fufa |4 oth | |
700 | 1 | |a Tang, Jianzhou |4 oth | |
700 | 1 | |a Zhou, Yi |4 oth | |
700 | 1 | |a Lu, Shuangqing |4 oth | |
700 | 1 | |a Zhang, Jianshe |4 oth | |
700 | 1 | |a Liu, Zhen |4 oth | |
700 | 1 | |a Zhou, Zhigang |4 oth | |
700 | 1 | |a Guo, Xinhong |4 oth | |
773 | 0 | 8 | |i Enthalten in |t Journal of the World Aquaculture Society |d Baton Rouge, La. : Soc., 1986 |g 48(2017), 2, Seite 333-341 |w (DE-627)165652276 |w (DE-600)233772-1 |w (DE-576)273832972 |x 0893-8849 |7 nnns |
773 | 1 | 8 | |g volume:48 |g year:2017 |g number:2 |g pages:333-341 |
856 | 4 | 1 | |u http://dx.doi.org/10.1111/jwas.12339 |3 Volltext |
856 | 4 | 2 | |u http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract |
856 | 4 | 2 | |u https://search.proquest.com/docview/1883397484 |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_OLC | ||
912 | |a SSG-OLC-GEO | ||
936 | b | k | |a 48.68 |q AVZ |
951 | |a AR | ||
952 | |d 48 |j 2017 |e 2 |h 333-341 |
author_variant |
r h rh |
---|---|
matchkey_str |
article:08938849:2017----::oeuacaatrztoaditrrgltootelsrytasotrigas |
hierarchy_sort_str |
2017 |
bklnumber |
48.68 |
publishDate |
2017 |
allfields |
10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341 |
spelling |
10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341 |
allfields_unstemmed |
10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341 |
allfieldsGer |
10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341 |
allfieldsSound |
10.1111/jwas.12339 doi PQ20170901 (DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe DE-627 ger DE-627 rakwb eng 550 ZDB 48.68 bkl Hu, Rong verfasserin aut Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. Nutzungsrecht: © Copyright by the World Aquaculture Society 2016 Gene expression Proteins Fish Zebrafish Wang, Yonghong oth Qu, Fufa oth Tang, Jianzhou oth Zhou, Yi oth Lu, Shuangqing oth Zhang, Jianshe oth Liu, Zhen oth Zhou, Zhigang oth Guo, Xinhong oth Enthalten in Journal of the World Aquaculture Society Baton Rouge, La. : Soc., 1986 48(2017), 2, Seite 333-341 (DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 0893-8849 nnns volume:48 year:2017 number:2 pages:333-341 http://dx.doi.org/10.1111/jwas.12339 Volltext http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO 48.68 AVZ AR 48 2017 2 333-341 |
language |
English |
source |
Enthalten in Journal of the World Aquaculture Society 48(2017), 2, Seite 333-341 volume:48 year:2017 number:2 pages:333-341 |
sourceStr |
Enthalten in Journal of the World Aquaculture Society 48(2017), 2, Seite 333-341 volume:48 year:2017 number:2 pages:333-341 |
format_phy_str_mv |
Article |
institution |
findex.gbv.de |
topic_facet |
Gene expression Proteins Fish Zebrafish |
dewey-raw |
550 |
isfreeaccess_bool |
false |
container_title |
Journal of the World Aquaculture Society |
authorswithroles_txt_mv |
Hu, Rong @@aut@@ Wang, Yonghong @@oth@@ Qu, Fufa @@oth@@ Tang, Jianzhou @@oth@@ Zhou, Yi @@oth@@ Lu, Shuangqing @@oth@@ Zhang, Jianshe @@oth@@ Liu, Zhen @@oth@@ Zhou, Zhigang @@oth@@ Guo, Xinhong @@oth@@ |
publishDateDaySort_date |
2017-01-01T00:00:00Z |
hierarchy_top_id |
165652276 |
dewey-sort |
3550 |
id |
OLC1991947208 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1991947208</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20220216135840.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">170512s2017 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/jwas.12339</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20170901</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1991947208</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1991947208</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">550</subfield><subfield code="q">ZDB</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">48.68</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hu, Rong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2017</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives.</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Nutzungsrecht: © Copyright by the World Aquaculture Society 2016</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gene expression</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteins</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fish</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Zebrafish</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Yonghong</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Qu, Fufa</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tang, Jianzhou</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhou, Yi</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Shuangqing</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Jianshe</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Zhen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhou, Zhigang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Guo, Xinhong</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of the World Aquaculture Society</subfield><subfield code="d">Baton Rouge, La. : Soc., 1986</subfield><subfield code="g">48(2017), 2, Seite 333-341</subfield><subfield code="w">(DE-627)165652276</subfield><subfield code="w">(DE-600)233772-1</subfield><subfield code="w">(DE-576)273832972</subfield><subfield code="x">0893-8849</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:48</subfield><subfield code="g">year:2017</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:333-341</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://dx.doi.org/10.1111/jwas.12339</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://search.proquest.com/docview/1883397484</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-GEO</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">48.68</subfield><subfield code="q">AVZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">48</subfield><subfield code="j">2017</subfield><subfield code="e">2</subfield><subfield code="h">333-341</subfield></datafield></record></collection>
|
author |
Hu, Rong |
spellingShingle |
Hu, Rong ddc 550 bkl 48.68 misc Gene expression misc Proteins misc Fish misc Zebrafish Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella |
authorStr |
Hu, Rong |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)165652276 |
format |
Article |
dewey-ones |
550 - Earth sciences |
delete_txt_mv |
keep |
author_role |
aut |
collection |
OLC |
remote_str |
false |
illustrated |
Not Illustrated |
issn |
0893-8849 |
topic_title |
550 ZDB 48.68 bkl Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella Gene expression Proteins Fish Zebrafish |
topic |
ddc 550 bkl 48.68 misc Gene expression misc Proteins misc Fish misc Zebrafish |
topic_unstemmed |
ddc 550 bkl 48.68 misc Gene expression misc Proteins misc Fish misc Zebrafish |
topic_browse |
ddc 550 bkl 48.68 misc Gene expression misc Proteins misc Fish misc Zebrafish |
format_facet |
Aufsätze Gedruckte Aufsätze |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
nc |
author2_variant |
y w yw f q fq j t jt y z yz s l sl j z jz z l zl z z zz x g xg |
hierarchy_parent_title |
Journal of the World Aquaculture Society |
hierarchy_parent_id |
165652276 |
dewey-tens |
550 - Earth sciences & geology |
hierarchy_top_title |
Journal of the World Aquaculture Society |
isfreeaccess_txt |
false |
familylinks_str_mv |
(DE-627)165652276 (DE-600)233772-1 (DE-576)273832972 |
title |
Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella |
ctrlnum |
(DE-627)OLC1991947208 (DE-599)GBVOLC1991947208 (PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70 (KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe |
title_full |
Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella |
author_sort |
Hu, Rong |
journal |
Journal of the World Aquaculture Society |
journalStr |
Journal of the World Aquaculture Society |
lang_code |
eng |
isOA_bool |
false |
dewey-hundreds |
500 - Science |
recordtype |
marc |
publishDateSort |
2017 |
contenttype_str_mv |
txt |
container_start_page |
333 |
author_browse |
Hu, Rong |
container_volume |
48 |
class |
550 ZDB 48.68 bkl |
format_se |
Aufsätze |
author-letter |
Hu, Rong |
doi_str_mv |
10.1111/jwas.12339 |
dewey-full |
550 |
title_sort |
molecular characterization and dietary regulation of the alasercys transporter 2 in grass carp, ctenopharyngodon idella |
title_auth |
Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella |
abstract |
AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. |
abstractGer |
AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. |
abstract_unstemmed |
AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives. |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-GEO |
container_issue |
2 |
title_short |
Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella |
url |
http://dx.doi.org/10.1111/jwas.12339 http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract https://search.proquest.com/docview/1883397484 |
remote_bool |
false |
author2 |
Wang, Yonghong Qu, Fufa Tang, Jianzhou Zhou, Yi Lu, Shuangqing Zhang, Jianshe Liu, Zhen Zhou, Zhigang Guo, Xinhong |
author2Str |
Wang, Yonghong Qu, Fufa Tang, Jianzhou Zhou, Yi Lu, Shuangqing Zhang, Jianshe Liu, Zhen Zhou, Zhigang Guo, Xinhong |
ppnlink |
165652276 |
mediatype_str_mv |
n |
isOA_txt |
false |
hochschulschrift_bool |
false |
author2_role |
oth oth oth oth oth oth oth oth oth |
doi_str |
10.1111/jwas.12339 |
up_date |
2024-07-04T03:56:53.288Z |
_version_ |
1803619310811217920 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1991947208</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20220216135840.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">170512s2017 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1111/jwas.12339</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20170901</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1991947208</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1991947208</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)p1889-e6927eae8d7df442265db79ef217c2baf547ace7bf01f449ee55ce78705c01a70</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0015053320170000048000200333molecularcharacterizationanddietaryregulationofthe</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">550</subfield><subfield code="q">ZDB</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">48.68</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hu, Rong</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Molecular Characterization and Dietary Regulation of the AlaSerCys Transporter 2 in Grass Carp, Ctenopharyngodon idella</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2017</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">AlaSerCys Transporter 2 ( ASCT2 ), a glutamine/amino acid transporter, plays an important role in the absorption of neutral amino acids. In this study, we cloned the full‐length cDNA of ASCT2 from grass carp, Ctenopharyngodon idella , which encodes a 541‐amino‐acid protein. Phylogenetic analysis revealed that the ASCT2 sequence of grass carp clustered with the ASCT2 from Danio rerio . ASCT2 mRNA was differentially expressed in different tissues, with a gradient expression from high to low in tissues of the liver, gill, muscle, midgut, hypophysis, hindgut, kidney, heart, foregut, brain, spleen, and gonad. Meanwhile, the quantitative real‐time polymerase chain reaction results showed that 22% crude protein diets could significantly stimulate ASCT2 gene expression. In addition, ASCT2 was expressed in higher quantities in the soybean meal group than in the fishmeal group. Furthermore, 7.5 g/kg glutamine and 2.5 g/kg glutamine dipeptide remarkably improved the expression of ASCT2 . These results suggested that ASCT2 expression is regulated by the dietary protein levels and sources, as well as appropriate concentration of glutamine and glutamine dipeptide additives.</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Nutzungsrecht: © Copyright by the World Aquaculture Society 2016</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Gene expression</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteins</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fish</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Zebrafish</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Yonghong</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Qu, Fufa</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Tang, Jianzhou</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhou, Yi</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Shuangqing</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Jianshe</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Zhen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhou, Zhigang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Guo, Xinhong</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of the World Aquaculture Society</subfield><subfield code="d">Baton Rouge, La. : Soc., 1986</subfield><subfield code="g">48(2017), 2, Seite 333-341</subfield><subfield code="w">(DE-627)165652276</subfield><subfield code="w">(DE-600)233772-1</subfield><subfield code="w">(DE-576)273832972</subfield><subfield code="x">0893-8849</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:48</subfield><subfield code="g">year:2017</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:333-341</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://dx.doi.org/10.1111/jwas.12339</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">http://onlinelibrary.wiley.com/doi/10.1111/jwas.12339/abstract</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://search.proquest.com/docview/1883397484</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-GEO</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">48.68</subfield><subfield code="q">AVZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">48</subfield><subfield code="j">2017</subfield><subfield code="e">2</subfield><subfield code="h">333-341</subfield></datafield></record></collection>
|
score |
7.400647 |