Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing

The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reductio...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Handlogten, Michael W [verfasserIn] Zhu, Min Ahuja, Sanjeev

Format:	Artikel
Sprache:	Englisch

Erschienen:	2017

Rechteinformationen:	Nutzungsrecht: © 2017 Wiley Periodicals, Inc.

Schlagwörter:	thioredoxin reductase disulfide bond reduction glutathione reductase glutathione thioredoxin antibody reduction Thioredoxin Reductases Mitigation Glutathione Monoclonal antibodies Enzymes Chemical bonds Manufacturing Pharmaceutical industry Cell culture Systems analysis Recombinant Reduction Bioprocessing Immunoglobulin G Reductase Pharmaceuticals Cell lines Biotechnology

Übergeordnetes Werk:	Enthalten in: Biotechnology and bioengineering - New York, NY [u.a.] : Wiley, 1962, 114(2017), 7, Seite 1469-1477
Übergeordnetes Werk:	volume:114 ; year:2017 ; number:7 ; pages:1469-1477

Links:	Volltext Link aufrufen Link aufrufen

DOI / URN:	10.1002/bit.26278

Katalog-ID:	OLC1994866926

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520			\|a The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics.
540			\|a Nutzungsrecht: © 2017 Wiley Periodicals, Inc.
650		4	\|a thioredoxin reductase
650		4	\|a disulfide bond reduction
650		4	\|a glutathione reductase
650		4	\|a glutathione
650		4	\|a thioredoxin
650		4	\|a antibody reduction
650		4	\|a Thioredoxin
650		4	\|a Reductases
650		4	\|a Mitigation
650		4	\|a Glutathione
650		4	\|a Monoclonal antibodies
650		4	\|a Enzymes
650		4	\|a Chemical bonds
650		4	\|a Manufacturing
650		4	\|a Pharmaceutical industry
650		4	\|a Cell culture
650		4	\|a Systems analysis
650		4	\|a Recombinant
650		4	\|a Reduction
650		4	\|a Bioprocessing
650		4	\|a Immunoglobulin G
650		4	\|a Reductase
650		4	\|a Pharmaceuticals
650		4	\|a Cell lines
650		4	\|a Biotechnology
700	1		\|a Zhu, Min \|4 oth
700	1		\|a Ahuja, Sanjeev \|4 oth
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allfields	10.1002/bit.26278 doi PQ20171228 (DE-627)OLC1994866926 (DE-599)GBVOLC1994866926 (PRQ)p1692-983bf5e35278e6041000e89249b31ba42b4e7c035bb8d08613d44241e6e608a23 (KEY)0118076220170000114000701469glutathioneandthioredoxinsystemscontributetorecomb DE-627 ger DE-627 rakwb eng 570 DE-600 BIODIV fid 58.30 bkl Handlogten, Michael W verfasserin aut Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics. Nutzungsrecht: © 2017 Wiley Periodicals, Inc. thioredoxin reductase disulfide bond reduction glutathione reductase glutathione thioredoxin antibody reduction Thioredoxin Reductases Mitigation Glutathione Monoclonal antibodies Enzymes Chemical bonds Manufacturing Pharmaceutical industry Cell culture Systems analysis Recombinant Reduction Bioprocessing Immunoglobulin G Reductase Pharmaceuticals Cell lines Biotechnology Zhu, Min oth Ahuja, Sanjeev oth Enthalten in Biotechnology and bioengineering New York, NY [u.a.] : Wiley, 1962 114(2017), 7, Seite 1469-1477 (DE-627)129851000 (DE-600)280318-5 (DE-576)015150194 0006-3592 nnns volume:114 year:2017 number:7 pages:1469-1477 http://dx.doi.org/10.1002/bit.26278 Volltext http://onlinelibrary.wiley.com/doi/10.1002/bit.26278/abstract https://search.proquest.com/docview/1902140035 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_215 GBV_ILN_4012 58.30 AVZ AR 114 2017 7 1469-1477
spelling	10.1002/bit.26278 doi PQ20171228 (DE-627)OLC1994866926 (DE-599)GBVOLC1994866926 (PRQ)p1692-983bf5e35278e6041000e89249b31ba42b4e7c035bb8d08613d44241e6e608a23 (KEY)0118076220170000114000701469glutathioneandthioredoxinsystemscontributetorecomb DE-627 ger DE-627 rakwb eng 570 DE-600 BIODIV fid 58.30 bkl Handlogten, Michael W verfasserin aut Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics. Nutzungsrecht: © 2017 Wiley Periodicals, Inc. thioredoxin reductase disulfide bond reduction glutathione reductase glutathione thioredoxin antibody reduction Thioredoxin Reductases Mitigation Glutathione Monoclonal antibodies Enzymes Chemical bonds Manufacturing Pharmaceutical industry Cell culture Systems analysis Recombinant Reduction Bioprocessing Immunoglobulin G Reductase Pharmaceuticals Cell lines Biotechnology Zhu, Min oth Ahuja, Sanjeev oth Enthalten in Biotechnology and bioengineering New York, NY [u.a.] : Wiley, 1962 114(2017), 7, Seite 1469-1477 (DE-627)129851000 (DE-600)280318-5 (DE-576)015150194 0006-3592 nnns volume:114 year:2017 number:7 pages:1469-1477 http://dx.doi.org/10.1002/bit.26278 Volltext http://onlinelibrary.wiley.com/doi/10.1002/bit.26278/abstract https://search.proquest.com/docview/1902140035 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_215 GBV_ILN_4012 58.30 AVZ AR 114 2017 7 1469-1477
allfields_unstemmed	10.1002/bit.26278 doi PQ20171228 (DE-627)OLC1994866926 (DE-599)GBVOLC1994866926 (PRQ)p1692-983bf5e35278e6041000e89249b31ba42b4e7c035bb8d08613d44241e6e608a23 (KEY)0118076220170000114000701469glutathioneandthioredoxinsystemscontributetorecomb DE-627 ger DE-627 rakwb eng 570 DE-600 BIODIV fid 58.30 bkl Handlogten, Michael W verfasserin aut Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics. Nutzungsrecht: © 2017 Wiley Periodicals, Inc. thioredoxin reductase disulfide bond reduction glutathione reductase glutathione thioredoxin antibody reduction Thioredoxin Reductases Mitigation Glutathione Monoclonal antibodies Enzymes Chemical bonds Manufacturing Pharmaceutical industry Cell culture Systems analysis Recombinant Reduction Bioprocessing Immunoglobulin G Reductase Pharmaceuticals Cell lines Biotechnology Zhu, Min oth Ahuja, Sanjeev oth Enthalten in Biotechnology and bioengineering New York, NY [u.a.] : Wiley, 1962 114(2017), 7, Seite 1469-1477 (DE-627)129851000 (DE-600)280318-5 (DE-576)015150194 0006-3592 nnns volume:114 year:2017 number:7 pages:1469-1477 http://dx.doi.org/10.1002/bit.26278 Volltext http://onlinelibrary.wiley.com/doi/10.1002/bit.26278/abstract https://search.proquest.com/docview/1902140035 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_215 GBV_ILN_4012 58.30 AVZ AR 114 2017 7 1469-1477
allfieldsGer	10.1002/bit.26278 doi PQ20171228 (DE-627)OLC1994866926 (DE-599)GBVOLC1994866926 (PRQ)p1692-983bf5e35278e6041000e89249b31ba42b4e7c035bb8d08613d44241e6e608a23 (KEY)0118076220170000114000701469glutathioneandthioredoxinsystemscontributetorecomb DE-627 ger DE-627 rakwb eng 570 DE-600 BIODIV fid 58.30 bkl Handlogten, Michael W verfasserin aut Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics. Nutzungsrecht: © 2017 Wiley Periodicals, Inc. thioredoxin reductase disulfide bond reduction glutathione reductase glutathione thioredoxin antibody reduction Thioredoxin Reductases Mitigation Glutathione Monoclonal antibodies Enzymes Chemical bonds Manufacturing Pharmaceutical industry Cell culture Systems analysis Recombinant Reduction Bioprocessing Immunoglobulin G Reductase Pharmaceuticals Cell lines Biotechnology Zhu, Min oth Ahuja, Sanjeev oth Enthalten in Biotechnology and bioengineering New York, NY [u.a.] : Wiley, 1962 114(2017), 7, Seite 1469-1477 (DE-627)129851000 (DE-600)280318-5 (DE-576)015150194 0006-3592 nnns volume:114 year:2017 number:7 pages:1469-1477 http://dx.doi.org/10.1002/bit.26278 Volltext http://onlinelibrary.wiley.com/doi/10.1002/bit.26278/abstract https://search.proquest.com/docview/1902140035 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_215 GBV_ILN_4012 58.30 AVZ AR 114 2017 7 1469-1477
allfieldsSound	10.1002/bit.26278 doi PQ20171228 (DE-627)OLC1994866926 (DE-599)GBVOLC1994866926 (PRQ)p1692-983bf5e35278e6041000e89249b31ba42b4e7c035bb8d08613d44241e6e608a23 (KEY)0118076220170000114000701469glutathioneandthioredoxinsystemscontributetorecomb DE-627 ger DE-627 rakwb eng 570 DE-600 BIODIV fid 58.30 bkl Handlogten, Michael W verfasserin aut Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics. Nutzungsrecht: © 2017 Wiley Periodicals, Inc. thioredoxin reductase disulfide bond reduction glutathione reductase glutathione thioredoxin antibody reduction Thioredoxin Reductases Mitigation Glutathione Monoclonal antibodies Enzymes Chemical bonds Manufacturing Pharmaceutical industry Cell culture Systems analysis Recombinant Reduction Bioprocessing Immunoglobulin G Reductase Pharmaceuticals Cell lines Biotechnology Zhu, Min oth Ahuja, Sanjeev oth Enthalten in Biotechnology and bioengineering New York, NY [u.a.] : Wiley, 1962 114(2017), 7, Seite 1469-1477 (DE-627)129851000 (DE-600)280318-5 (DE-576)015150194 0006-3592 nnns volume:114 year:2017 number:7 pages:1469-1477 http://dx.doi.org/10.1002/bit.26278 Volltext http://onlinelibrary.wiley.com/doi/10.1002/bit.26278/abstract https://search.proquest.com/docview/1902140035 GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 SSG-OPC-FOR GBV_ILN_70 GBV_ILN_215 GBV_ILN_4012 58.30 AVZ AR 114 2017 7 1469-1477
language	English
source	Enthalten in Biotechnology and bioengineering 114(2017), 7, Seite 1469-1477 volume:114 year:2017 number:7 pages:1469-1477
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institution	findex.gbv.de
topic_facet	thioredoxin reductase disulfide bond reduction glutathione reductase glutathione thioredoxin antibody reduction Thioredoxin Reductases Mitigation Glutathione Monoclonal antibodies Enzymes Chemical bonds Manufacturing Pharmaceutical industry Cell culture Systems analysis Recombinant Reduction Bioprocessing Immunoglobulin G Reductase Pharmaceuticals Cell lines Biotechnology
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author	Handlogten, Michael W
spellingShingle	Handlogten, Michael W ddc 570 fid BIODIV bkl 58.30 misc thioredoxin reductase misc disulfide bond reduction misc glutathione reductase misc glutathione misc thioredoxin misc antibody reduction misc Thioredoxin misc Reductases misc Mitigation misc Glutathione misc Monoclonal antibodies misc Enzymes misc Chemical bonds misc Manufacturing misc Pharmaceutical industry misc Cell culture misc Systems analysis misc Recombinant misc Reduction misc Bioprocessing misc Immunoglobulin G misc Reductase misc Pharmaceuticals misc Cell lines misc Biotechnology Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing
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topic_title	570 DE-600 BIODIV fid 58.30 bkl Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing thioredoxin reductase disulfide bond reduction glutathione reductase glutathione thioredoxin antibody reduction Thioredoxin Reductases Mitigation Glutathione Monoclonal antibodies Enzymes Chemical bonds Manufacturing Pharmaceutical industry Cell culture Systems analysis Recombinant Reduction Bioprocessing Immunoglobulin G Reductase Pharmaceuticals Cell lines Biotechnology
topic	ddc 570 fid BIODIV bkl 58.30 misc thioredoxin reductase misc disulfide bond reduction misc glutathione reductase misc glutathione misc thioredoxin misc antibody reduction misc Thioredoxin misc Reductases misc Mitigation misc Glutathione misc Monoclonal antibodies misc Enzymes misc Chemical bonds misc Manufacturing misc Pharmaceutical industry misc Cell culture misc Systems analysis misc Recombinant misc Reduction misc Bioprocessing misc Immunoglobulin G misc Reductase misc Pharmaceuticals misc Cell lines misc Biotechnology
topic_unstemmed	ddc 570 fid BIODIV bkl 58.30 misc thioredoxin reductase misc disulfide bond reduction misc glutathione reductase misc glutathione misc thioredoxin misc antibody reduction misc Thioredoxin misc Reductases misc Mitigation misc Glutathione misc Monoclonal antibodies misc Enzymes misc Chemical bonds misc Manufacturing misc Pharmaceutical industry misc Cell culture misc Systems analysis misc Recombinant misc Reduction misc Bioprocessing misc Immunoglobulin G misc Reductase misc Pharmaceuticals misc Cell lines misc Biotechnology
topic_browse	ddc 570 fid BIODIV bkl 58.30 misc thioredoxin reductase misc disulfide bond reduction misc glutathione reductase misc glutathione misc thioredoxin misc antibody reduction misc Thioredoxin misc Reductases misc Mitigation misc Glutathione misc Monoclonal antibodies misc Enzymes misc Chemical bonds misc Manufacturing misc Pharmaceutical industry misc Cell culture misc Systems analysis misc Recombinant misc Reduction misc Bioprocessing misc Immunoglobulin G misc Reductase misc Pharmaceuticals misc Cell lines misc Biotechnology
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title	Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing
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title_full	Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing
author_sort	Handlogten, Michael W
journal	Biotechnology and bioengineering
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title_sort	glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing
title_auth	Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing
abstract	The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics.
abstractGer	The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics.
abstract_unstemmed	The reduction of antibody interchain disulfide bonds (antibody reduction) during bioprocessing leads to loss of product, requires more complex purification processes, and can lead to product instability. To date, the thioredoxin system has been identified as the sole contributor to antibody reduction, however our results demonstrate that both the glutathione and thioredoxin systems contribute to antibody reduction. This knowledge can be used to tailor reduction mitigation strategies to the dominant pathway, ensuring consistency in product quality during production of biotherapeutics.
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container_issue	7
title_short	Glutathione and thioredoxin systems contribute to recombinant monoclonal antibody interchain disulfide bond reduction during bioprocessing
url	http://dx.doi.org/10.1002/bit.26278 http://onlinelibrary.wiley.com/doi/10.1002/bit.26278/abstract https://search.proquest.com/docview/1902140035
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author2	Zhu, Min Ahuja, Sanjeev
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doi_str	10.1002/bit.26278
up_date	2024-07-03T19:33:17.062Z
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