Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei
As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Hou, YunHua [verfasserIn] |
|---|
| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2017 |
|---|
| Rechteinformationen: |
Nutzungsrecht: © Springer Science+Business Media, LLC 2017 |
|---|
| Schlagwörter: |
|---|
| Übergeordnetes Werk: |
Enthalten in: Applied biochemistry and biotechnology / A - New York, NY : Springer, 1994, 183(2017), 1, Seite 254-264 |
|---|---|
| Übergeordnetes Werk: |
volume:183 ; year:2017 ; number:1 ; pages:254-264 |
| Links: |
|---|
| DOI / URN: |
10.1007/s12010-017-2442-3 |
|---|
| Katalog-ID: |
OLC1997417553 |
|---|
| LEADER | 01000caa a2200265 4500 | ||
|---|---|---|---|
| 001 | OLC1997417553 | ||
| 003 | DE-627 | ||
| 005 | 20230513181517.0 | ||
| 007 | tu | ||
| 008 | 171125s2017 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s12010-017-2442-3 |2 doi | |
| 028 | 5 | 2 | |a PQ20171125 |
| 035 | |a (DE-627)OLC1997417553 | ||
| 035 | |a (DE-599)GBVOLC1997417553 | ||
| 035 | |a (PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0 | ||
| 035 | |a (KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 570 |a 540 |a 660 |q DE-600 |
| 084 | |a 42.00 |2 bkl | ||
| 100 | 1 | |a Hou, YunHua |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei |
| 264 | 1 | |c 2017 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 520 | |a As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. | ||
| 540 | |a Nutzungsrecht: © Springer Science+Business Media, LLC 2017 | ||
| 650 | 4 | |a Biochemistry, general | |
| 650 | 4 | |a β-glucosidase | |
| 650 | 4 | |a Feruloyl esterase | |
| 650 | 4 | |a Chemistry | |
| 650 | 4 | |a Trichoderma reesei | |
| 650 | 4 | |a Biotechnology | |
| 650 | 4 | |a Random insertion strategy | |
| 700 | 1 | |a Pan, Yang |4 oth | |
| 700 | 1 | |a Yan, MengJie |4 oth | |
| 700 | 1 | |a He, Huan |4 oth | |
| 700 | 1 | |a Yang, QinZheng |4 oth | |
| 700 | 1 | |a Zhong, YaoHua |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |t Applied biochemistry and biotechnology / A |d New York, NY : Springer, 1994 |g 183(2017), 1, Seite 254-264 |w (DE-627)182278573 |w (DE-600)1193054-8 |w (DE-576)043085105 |x 0273-2289 |7 nnns |
| 773 | 1 | 8 | |g volume:183 |g year:2017 |g number:1 |g pages:254-264 |
| 856 | 4 | 1 | |u http://dx.doi.org/10.1007/s12010-017-2442-3 |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-TEC | ||
| 912 | |a SSG-OLC-CHE | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_4012 | ||
| 936 | b | k | |a 42.00 |q AVZ |
| 951 | |a AR | ||
| 952 | |d 183 |j 2017 |e 1 |h 254-264 | ||
| author_variant |
y h yh |
|---|---|
| matchkey_str |
article:02732289:2017----::nlecornolisrefrlyetrsanlcsdsatv |
| hierarchy_sort_str |
2017 |
| bklnumber |
42.00 |
| publishDate |
2017 |
| allfields |
10.1007/s12010-017-2442-3 doi PQ20171125 (DE-627)OLC1997417553 (DE-599)GBVOLC1997417553 (PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0 (KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc DE-627 ger DE-627 rakwb eng 570 540 660 DE-600 42.00 bkl Hou, YunHua verfasserin aut Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. Nutzungsrecht: © Springer Science+Business Media, LLC 2017 Biochemistry, general β-glucosidase Feruloyl esterase Chemistry Trichoderma reesei Biotechnology Random insertion strategy Pan, Yang oth Yan, MengJie oth He, Huan oth Yang, QinZheng oth Zhong, YaoHua oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 183(2017), 1, Seite 254-264 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:183 year:2017 number:1 pages:254-264 http://dx.doi.org/10.1007/s12010-017-2442-3 Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 183 2017 1 254-264 |
| spelling |
10.1007/s12010-017-2442-3 doi PQ20171125 (DE-627)OLC1997417553 (DE-599)GBVOLC1997417553 (PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0 (KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc DE-627 ger DE-627 rakwb eng 570 540 660 DE-600 42.00 bkl Hou, YunHua verfasserin aut Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. Nutzungsrecht: © Springer Science+Business Media, LLC 2017 Biochemistry, general β-glucosidase Feruloyl esterase Chemistry Trichoderma reesei Biotechnology Random insertion strategy Pan, Yang oth Yan, MengJie oth He, Huan oth Yang, QinZheng oth Zhong, YaoHua oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 183(2017), 1, Seite 254-264 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:183 year:2017 number:1 pages:254-264 http://dx.doi.org/10.1007/s12010-017-2442-3 Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 183 2017 1 254-264 |
| allfields_unstemmed |
10.1007/s12010-017-2442-3 doi PQ20171125 (DE-627)OLC1997417553 (DE-599)GBVOLC1997417553 (PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0 (KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc DE-627 ger DE-627 rakwb eng 570 540 660 DE-600 42.00 bkl Hou, YunHua verfasserin aut Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. Nutzungsrecht: © Springer Science+Business Media, LLC 2017 Biochemistry, general β-glucosidase Feruloyl esterase Chemistry Trichoderma reesei Biotechnology Random insertion strategy Pan, Yang oth Yan, MengJie oth He, Huan oth Yang, QinZheng oth Zhong, YaoHua oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 183(2017), 1, Seite 254-264 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:183 year:2017 number:1 pages:254-264 http://dx.doi.org/10.1007/s12010-017-2442-3 Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 183 2017 1 254-264 |
| allfieldsGer |
10.1007/s12010-017-2442-3 doi PQ20171125 (DE-627)OLC1997417553 (DE-599)GBVOLC1997417553 (PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0 (KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc DE-627 ger DE-627 rakwb eng 570 540 660 DE-600 42.00 bkl Hou, YunHua verfasserin aut Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. Nutzungsrecht: © Springer Science+Business Media, LLC 2017 Biochemistry, general β-glucosidase Feruloyl esterase Chemistry Trichoderma reesei Biotechnology Random insertion strategy Pan, Yang oth Yan, MengJie oth He, Huan oth Yang, QinZheng oth Zhong, YaoHua oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 183(2017), 1, Seite 254-264 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:183 year:2017 number:1 pages:254-264 http://dx.doi.org/10.1007/s12010-017-2442-3 Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 183 2017 1 254-264 |
| allfieldsSound |
10.1007/s12010-017-2442-3 doi PQ20171125 (DE-627)OLC1997417553 (DE-599)GBVOLC1997417553 (PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0 (KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc DE-627 ger DE-627 rakwb eng 570 540 660 DE-600 42.00 bkl Hou, YunHua verfasserin aut Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. Nutzungsrecht: © Springer Science+Business Media, LLC 2017 Biochemistry, general β-glucosidase Feruloyl esterase Chemistry Trichoderma reesei Biotechnology Random insertion strategy Pan, Yang oth Yan, MengJie oth He, Huan oth Yang, QinZheng oth Zhong, YaoHua oth Enthalten in Applied biochemistry and biotechnology / A New York, NY : Springer, 1994 183(2017), 1, Seite 254-264 (DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 0273-2289 nnns volume:183 year:2017 number:1 pages:254-264 http://dx.doi.org/10.1007/s12010-017-2442-3 Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_4012 42.00 AVZ AR 183 2017 1 254-264 |
| language |
English |
| source |
Enthalten in Applied biochemistry and biotechnology / A 183(2017), 1, Seite 254-264 volume:183 year:2017 number:1 pages:254-264 |
| sourceStr |
Enthalten in Applied biochemistry and biotechnology / A 183(2017), 1, Seite 254-264 volume:183 year:2017 number:1 pages:254-264 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Biochemistry, general β-glucosidase Feruloyl esterase Chemistry Trichoderma reesei Biotechnology Random insertion strategy |
| dewey-raw |
570 |
| isfreeaccess_bool |
false |
| container_title |
Applied biochemistry and biotechnology / A |
| authorswithroles_txt_mv |
Hou, YunHua @@aut@@ Pan, Yang @@oth@@ Yan, MengJie @@oth@@ He, Huan @@oth@@ Yang, QinZheng @@oth@@ Zhong, YaoHua @@oth@@ |
| publishDateDaySort_date |
2017-01-01T00:00:00Z |
| hierarchy_top_id |
182278573 |
| dewey-sort |
3570 |
| id |
OLC1997417553 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1997417553</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230513181517.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">171125s2017 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s12010-017-2442-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20171125</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1997417553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1997417553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">540</subfield><subfield code="a">660</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hou, YunHua</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2017</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi.</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Nutzungsrecht: © Springer Science+Business Media, LLC 2017</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry, general</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">β-glucosidase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Feruloyl esterase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Trichoderma reesei</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biotechnology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Random insertion strategy</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pan, Yang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yan, MengJie</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">He, Huan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, QinZheng</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhong, YaoHua</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Applied biochemistry and biotechnology / A</subfield><subfield code="d">New York, NY : Springer, 1994</subfield><subfield code="g">183(2017), 1, Seite 254-264</subfield><subfield code="w">(DE-627)182278573</subfield><subfield code="w">(DE-600)1193054-8</subfield><subfield code="w">(DE-576)043085105</subfield><subfield code="x">0273-2289</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:183</subfield><subfield code="g">year:2017</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:254-264</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://dx.doi.org/10.1007/s12010-017-2442-3</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.00</subfield><subfield code="q">AVZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">183</subfield><subfield code="j">2017</subfield><subfield code="e">1</subfield><subfield code="h">254-264</subfield></datafield></record></collection>
|
| author |
Hou, YunHua |
| spellingShingle |
Hou, YunHua ddc 570 bkl 42.00 misc Biochemistry, general misc β-glucosidase misc Feruloyl esterase misc Chemistry misc Trichoderma reesei misc Biotechnology misc Random insertion strategy Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei |
| authorStr |
Hou, YunHua |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)182278573 |
| format |
Article |
| dewey-ones |
570 - Life sciences; biology 540 - Chemistry & allied sciences 660 - Chemical engineering |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
OLC |
| remote_str |
false |
| illustrated |
Not Illustrated |
| issn |
0273-2289 |
| topic_title |
570 540 660 DE-600 42.00 bkl Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei Biochemistry, general β-glucosidase Feruloyl esterase Chemistry Trichoderma reesei Biotechnology Random insertion strategy |
| topic |
ddc 570 bkl 42.00 misc Biochemistry, general misc β-glucosidase misc Feruloyl esterase misc Chemistry misc Trichoderma reesei misc Biotechnology misc Random insertion strategy |
| topic_unstemmed |
ddc 570 bkl 42.00 misc Biochemistry, general misc β-glucosidase misc Feruloyl esterase misc Chemistry misc Trichoderma reesei misc Biotechnology misc Random insertion strategy |
| topic_browse |
ddc 570 bkl 42.00 misc Biochemistry, general misc β-glucosidase misc Feruloyl esterase misc Chemistry misc Trichoderma reesei misc Biotechnology misc Random insertion strategy |
| format_facet |
Aufsätze Gedruckte Aufsätze |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
nc |
| author2_variant |
y p yp m y my h h hh q y qy y z yz |
| hierarchy_parent_title |
Applied biochemistry and biotechnology / A |
| hierarchy_parent_id |
182278573 |
| dewey-tens |
570 - Life sciences; biology 540 - Chemistry 660 - Chemical engineering |
| hierarchy_top_title |
Applied biochemistry and biotechnology / A |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)182278573 (DE-600)1193054-8 (DE-576)043085105 |
| title |
Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei |
| ctrlnum |
(DE-627)OLC1997417553 (DE-599)GBVOLC1997417553 (PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0 (KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc |
| title_full |
Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei |
| author_sort |
Hou, YunHua |
| journal |
Applied biochemistry and biotechnology / A |
| journalStr |
Applied biochemistry and biotechnology / A |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
500 - Science 600 - Technology |
| recordtype |
marc |
| publishDateSort |
2017 |
| contenttype_str_mv |
txt |
| container_start_page |
254 |
| author_browse |
Hou, YunHua |
| container_volume |
183 |
| class |
570 540 660 DE-600 42.00 bkl |
| format_se |
Aufsätze |
| author-letter |
Hou, YunHua |
| doi_str_mv |
10.1007/s12010-017-2442-3 |
| dewey-full |
570 540 660 |
| title_sort |
influence of randomly inserted feruloyl esterase a on β-glucosidase activity in trichoderma reesei |
| title_auth |
Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei |
| abstract |
As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. |
| abstractGer |
As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. |
| abstract_unstemmed |
As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi. |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_4012 |
| container_issue |
1 |
| title_short |
Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei |
| url |
http://dx.doi.org/10.1007/s12010-017-2442-3 |
| remote_bool |
false |
| author2 |
Pan, Yang Yan, MengJie He, Huan Yang, QinZheng Zhong, YaoHua |
| author2Str |
Pan, Yang Yan, MengJie He, Huan Yang, QinZheng Zhong, YaoHua |
| ppnlink |
182278573 |
| mediatype_str_mv |
n |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth |
| doi_str |
10.1007/s12010-017-2442-3 |
| up_date |
2024-07-04T02:51:18.669Z |
| _version_ |
1803615185068359680 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a2200265 4500</leader><controlfield tag="001">OLC1997417553</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230513181517.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">171125s2017 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s12010-017-2442-3</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">PQ20171125</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC1997417553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)GBVOLC1997417553</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(PRQ)c1406-14c95af2dd6e94a4c94d56dce28171b37018f0b9f123c9890a731c30daca2b8f0</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(KEY)0068751020170000183000100254influenceofrandomlyinsertedferuloylesteraseaongluc</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">540</subfield><subfield code="a">660</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">42.00</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hou, YunHua</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Influence of Randomly Inserted Feruloyl Esterase A on β-Glucosidase Activity in Trichoderma reesei</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2017</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">As a well-known industrial fungus for cellulase production, the strain RUT-C30 of Trichoderma reesei was selected to produce the feruloyl esterase A (FAEA) by a random integration protocol. The strong promoter of cellobiohydrolase 1 (cbh1) gene was used to drive the expression of FAEA. Using double-joint PCR protocol, Pcbh1-faeA-TtrpC expression cassette was successfully constructed and co-transformed into RUT C30 strain of T. reesei. One transformant with high feruloyl esterase yield (3.44 ± 0.16 IU/mL) was obtained through plate screening and named TrfaeA1. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of fermentation supernatant from transformant TrfaeA1 showed a distinct protein band appearing at the position of about 34 kDa, indicating that faeA gene has been successfully expressed in T. reesei. Compared with that in original RUT C30 strain, β-glucosidase production in transformant TrfaeA1 was significantly increased by about 86.4%, reaching 63.2 IU/mL due to the random insertion of faeA. Moreover, the total secretion protein and filter paper activities of the transformant TrfaeA1 were also improved by up to 5.5 and 4.3%, respectively. The present results indicated that the random insertion strategy could be an effective and feasible method to improve and optimize the cellulase system of filamentous fungi.</subfield></datafield><datafield tag="540" ind1=" " ind2=" "><subfield code="a">Nutzungsrecht: © Springer Science+Business Media, LLC 2017</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biochemistry, general</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">β-glucosidase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Feruloyl esterase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chemistry</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Trichoderma reesei</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Biotechnology</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Random insertion strategy</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pan, Yang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yan, MengJie</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">He, Huan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yang, QinZheng</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhong, YaoHua</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Applied biochemistry and biotechnology / A</subfield><subfield code="d">New York, NY : Springer, 1994</subfield><subfield code="g">183(2017), 1, Seite 254-264</subfield><subfield code="w">(DE-627)182278573</subfield><subfield code="w">(DE-600)1193054-8</subfield><subfield code="w">(DE-576)043085105</subfield><subfield code="x">0273-2289</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:183</subfield><subfield code="g">year:2017</subfield><subfield code="g">number:1</subfield><subfield code="g">pages:254-264</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">http://dx.doi.org/10.1007/s12010-017-2442-3</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">42.00</subfield><subfield code="q">AVZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">183</subfield><subfield code="j">2017</subfield><subfield code="e">1</subfield><subfield code="h">254-264</subfield></datafield></record></collection>
|
| score |
7.399269 |