Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays
We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cell...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lee, Jong Min [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Rechteinformationen: |
Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Electrophoresis - Weinheim : Wiley-VCH, 1980, 38(2017), 24, Seite 3161-3167 |
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| Übergeordnetes Werk: |
volume:38 ; year:2017 ; number:24 ; pages:3161-3167 |
| Links: |
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| DOI / URN: |
10.1002/elps.201700118 |
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| Katalog-ID: |
OLC1999509463 |
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| 520 | |a We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. | ||
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| 650 | 4 | |a Uniform‐sized neurosphere | |
| 650 | 4 | |a Hydrogel microwell array | |
| 650 | 4 | |a Motoneuron differentiation | |
| 700 | 1 | |a Moon, Joo Yoon |4 oth | |
| 700 | 1 | |a Shaker, Mohammed R |4 oth | |
| 700 | 1 | |a Sun, Woong |4 oth | |
| 700 | 1 | |a Chung, Bong Geun |4 oth | |
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10.1002/elps.201700118 doi PQ20171228 (DE-627)OLC1999509463 (DE-599)GBVOLC1999509463 (PRQ)p1109-f0da534b262dcee8e0d044a1a452fb5cd5b973c98dddf2e38bac81ecb938dbcd3 (KEY)0204026320170000038002403161uniformsizedneurospheremediatedmotoneurondifferent DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Lee, Jong Min verfasserin aut Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Uniform‐sized neurosphere Hydrogel microwell array Motoneuron differentiation Moon, Joo Yoon oth Shaker, Mohammed R oth Sun, Woong oth Chung, Bong Geun oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3161-3167 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3161-3167 http://dx.doi.org/10.1002/elps.201700118 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700118/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3161-3167 |
| spelling |
10.1002/elps.201700118 doi PQ20171228 (DE-627)OLC1999509463 (DE-599)GBVOLC1999509463 (PRQ)p1109-f0da534b262dcee8e0d044a1a452fb5cd5b973c98dddf2e38bac81ecb938dbcd3 (KEY)0204026320170000038002403161uniformsizedneurospheremediatedmotoneurondifferent DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Lee, Jong Min verfasserin aut Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Uniform‐sized neurosphere Hydrogel microwell array Motoneuron differentiation Moon, Joo Yoon oth Shaker, Mohammed R oth Sun, Woong oth Chung, Bong Geun oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3161-3167 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3161-3167 http://dx.doi.org/10.1002/elps.201700118 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700118/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3161-3167 |
| allfields_unstemmed |
10.1002/elps.201700118 doi PQ20171228 (DE-627)OLC1999509463 (DE-599)GBVOLC1999509463 (PRQ)p1109-f0da534b262dcee8e0d044a1a452fb5cd5b973c98dddf2e38bac81ecb938dbcd3 (KEY)0204026320170000038002403161uniformsizedneurospheremediatedmotoneurondifferent DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Lee, Jong Min verfasserin aut Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Uniform‐sized neurosphere Hydrogel microwell array Motoneuron differentiation Moon, Joo Yoon oth Shaker, Mohammed R oth Sun, Woong oth Chung, Bong Geun oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3161-3167 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3161-3167 http://dx.doi.org/10.1002/elps.201700118 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700118/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3161-3167 |
| allfieldsGer |
10.1002/elps.201700118 doi PQ20171228 (DE-627)OLC1999509463 (DE-599)GBVOLC1999509463 (PRQ)p1109-f0da534b262dcee8e0d044a1a452fb5cd5b973c98dddf2e38bac81ecb938dbcd3 (KEY)0204026320170000038002403161uniformsizedneurospheremediatedmotoneurondifferent DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Lee, Jong Min verfasserin aut Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Uniform‐sized neurosphere Hydrogel microwell array Motoneuron differentiation Moon, Joo Yoon oth Shaker, Mohammed R oth Sun, Woong oth Chung, Bong Geun oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3161-3167 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3161-3167 http://dx.doi.org/10.1002/elps.201700118 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700118/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3161-3167 |
| allfieldsSound |
10.1002/elps.201700118 doi PQ20171228 (DE-627)OLC1999509463 (DE-599)GBVOLC1999509463 (PRQ)p1109-f0da534b262dcee8e0d044a1a452fb5cd5b973c98dddf2e38bac81ecb938dbcd3 (KEY)0204026320170000038002403161uniformsizedneurospheremediatedmotoneurondifferent DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Lee, Jong Min verfasserin aut Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Uniform‐sized neurosphere Hydrogel microwell array Motoneuron differentiation Moon, Joo Yoon oth Shaker, Mohammed R oth Sun, Woong oth Chung, Bong Geun oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3161-3167 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3161-3167 http://dx.doi.org/10.1002/elps.201700118 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700118/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3161-3167 |
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Enthalten in Electrophoresis 38(2017), 24, Seite 3161-3167 volume:38 year:2017 number:24 pages:3161-3167 |
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Lee, Jong Min @@aut@@ Moon, Joo Yoon @@oth@@ Shaker, Mohammed R @@oth@@ Sun, Woong @@oth@@ Chung, Bong Geun @@oth@@ |
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Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays |
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We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. |
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We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. |
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We developed the photocrosslinkable hydrogel microwell arrays for uniform‐sized neurosphere‐mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform‐sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform‐sized neurospheres harvested from microwell arrays were replated into laminin‐coated substrate. In parallel, uniform‐sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three‐dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform‐sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform‐sized neurosphere‐mediated motoneuron differentiation. |
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Uniform‐sized neurosphere‐mediated motoneuron differentiation in microwell arrays |
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