A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis
Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with po...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yu, Bing [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Rechteinformationen: |
Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Electrophoresis - Weinheim : Wiley-VCH, 1980, 38(2017), 24, Seite 3104-3110 |
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| Übergeordnetes Werk: |
volume:38 ; year:2017 ; number:24 ; pages:3104-3110 |
| Links: |
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| DOI / URN: |
10.1002/elps.201700249 |
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OLC1999509498 |
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| 245 | 1 | 2 | |a A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis |
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| 520 | |a Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. | ||
| 540 | |a Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim | ||
| 650 | 4 | |a Capillary electrophoresis | |
| 650 | 4 | |a Covalent coating | |
| 650 | 4 | |a Polyglycerol dendrimer | |
| 650 | 4 | |a Diazoresin | |
| 650 | 4 | |a Anti‐protein‐fouling | |
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| 700 | 1 | |a Cong, Hailin |4 oth | |
| 700 | 1 | |a Li, Guoling |4 oth | |
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10.1002/elps.201700249 doi PQ20171228 (DE-627)OLC1999509498 (DE-599)GBVOLC1999509498 (PRQ)p1390-98d89956484f7a19f76a6b14df5c38fb1c05c6bcfcc55ab2c0920ce261bc60403 (KEY)0204026320170000038002403104covalentcapillarycoatingofdiazoresinandpolyglycero DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Yu, Bing verfasserin aut A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Capillary electrophoresis Covalent coating Polyglycerol dendrimer Diazoresin Anti‐protein‐fouling Wang, Minghong oth Cong, Hailin oth Li, Guoling oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3104-3110 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3104-3110 http://dx.doi.org/10.1002/elps.201700249 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700249/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3104-3110 |
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10.1002/elps.201700249 doi PQ20171228 (DE-627)OLC1999509498 (DE-599)GBVOLC1999509498 (PRQ)p1390-98d89956484f7a19f76a6b14df5c38fb1c05c6bcfcc55ab2c0920ce261bc60403 (KEY)0204026320170000038002403104covalentcapillarycoatingofdiazoresinandpolyglycero DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Yu, Bing verfasserin aut A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Capillary electrophoresis Covalent coating Polyglycerol dendrimer Diazoresin Anti‐protein‐fouling Wang, Minghong oth Cong, Hailin oth Li, Guoling oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3104-3110 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3104-3110 http://dx.doi.org/10.1002/elps.201700249 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700249/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3104-3110 |
| allfields_unstemmed |
10.1002/elps.201700249 doi PQ20171228 (DE-627)OLC1999509498 (DE-599)GBVOLC1999509498 (PRQ)p1390-98d89956484f7a19f76a6b14df5c38fb1c05c6bcfcc55ab2c0920ce261bc60403 (KEY)0204026320170000038002403104covalentcapillarycoatingofdiazoresinandpolyglycero DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Yu, Bing verfasserin aut A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Capillary electrophoresis Covalent coating Polyglycerol dendrimer Diazoresin Anti‐protein‐fouling Wang, Minghong oth Cong, Hailin oth Li, Guoling oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3104-3110 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3104-3110 http://dx.doi.org/10.1002/elps.201700249 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700249/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3104-3110 |
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10.1002/elps.201700249 doi PQ20171228 (DE-627)OLC1999509498 (DE-599)GBVOLC1999509498 (PRQ)p1390-98d89956484f7a19f76a6b14df5c38fb1c05c6bcfcc55ab2c0920ce261bc60403 (KEY)0204026320170000038002403104covalentcapillarycoatingofdiazoresinandpolyglycero DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Yu, Bing verfasserin aut A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Capillary electrophoresis Covalent coating Polyglycerol dendrimer Diazoresin Anti‐protein‐fouling Wang, Minghong oth Cong, Hailin oth Li, Guoling oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3104-3110 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3104-3110 http://dx.doi.org/10.1002/elps.201700249 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700249/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3104-3110 |
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10.1002/elps.201700249 doi PQ20171228 (DE-627)OLC1999509498 (DE-599)GBVOLC1999509498 (PRQ)p1390-98d89956484f7a19f76a6b14df5c38fb1c05c6bcfcc55ab2c0920ce261bc60403 (KEY)0204026320170000038002403104covalentcapillarycoatingofdiazoresinandpolyglycero DE-627 ger DE-627 rakwb eng 540 570 DE-101 570 AVZ BIODIV fid 35.29 bkl Yu, Bing verfasserin aut A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis 2017 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. Nutzungsrecht: © 2017 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim Capillary electrophoresis Covalent coating Polyglycerol dendrimer Diazoresin Anti‐protein‐fouling Wang, Minghong oth Cong, Hailin oth Li, Guoling oth Enthalten in Electrophoresis Weinheim : Wiley-VCH, 1980 38(2017), 24, Seite 3104-3110 (DE-627)130409952 (DE-600)619001-7 (DE-576)015913732 0173-0835 nnns volume:38 year:2017 number:24 pages:3104-3110 http://dx.doi.org/10.1002/elps.201700249 Volltext http://onlinelibrary.wiley.com/doi/10.1002/elps.201700249/abstract GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2219 GBV_ILN_4012 35.29 AVZ AR 38 2017 24 3104-3110 |
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covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis |
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A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis |
| abstract |
Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. |
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Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. |
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Overcoming proteins adsorption on the inner surface of capillary has attracted increasing attention recently. By using the unique photochemistry reaction of diazoresin (DR), a new covalent capillary coating was prepared on the fused‐silica capillary through layer‐by‐layer self‐assembly of DR with polyglycerol (PG) dendrimer. The separation performance of covalently DR/PG‐dendrimer coated capillary noticeably exceeded the bare capillary and the noncovalently linked DR/PG‐dendrimer capillary. A baseline separation of lysozyme, myoglobin, bovine serum albumin, and ribonuclease A was achieved using CE within 20 min. Besides, the covalently linked DR/PG‐dendrimer coating has the remarkable stability and reproducibility. Especially, compared with the traditional method which use highly toxic and moisture‐sensitive silane coupling agent, this method seems to be a simple and environmental friendly way to prepare the covalently coated capillaries for CE. |
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A covalent capillary coating of diazoresin and polyglycerol dendrimer for protein analysis using capillary electrophoresis |
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