Confocal Microscopy Imaging with an Optical Transition Edge Sensor
Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photo...
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Gespeichert in:
| Autor*in: |
Fukuda, D. [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC, part of Springer Nature 2018 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of low temperature physics - Springer US, 1969, 193(2018), 5-6 vom: 10. Mai, Seite 1228-1235 |
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| Übergeordnetes Werk: |
volume:193 ; year:2018 ; number:5-6 ; day:10 ; month:05 ; pages:1228-1235 |
| Links: |
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| DOI / URN: |
10.1007/s10909-018-1938-8 |
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| Katalog-ID: |
OLC2036831931 |
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| 520 | |a Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below $$1\,\mu \hbox {W}$$. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate ($$<\,1$$ Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes. | ||
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10.1007/s10909-018-1938-8 doi (DE-627)OLC2036831931 (DE-He213)s10909-018-1938-8-p DE-627 ger DE-627 rakwb eng 530 VZ Fukuda, D. verfasserin aut Confocal Microscopy Imaging with an Optical Transition Edge Sensor 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below $$1\,\mu \hbox {W}$$. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate ($$<\,1$$ Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes. Fluorescence Photobleaching Dark count Detection efficiency Resolution Niwa, K. aut Hattori, K. aut Inoue, S. aut Kobayashi, R. aut Numata, T. aut Enthalten in Journal of low temperature physics Springer US, 1969 193(2018), 5-6 vom: 10. Mai, Seite 1228-1235 (DE-627)129546267 (DE-600)218311-0 (DE-576)014996642 0022-2291 nnns volume:193 year:2018 number:5-6 day:10 month:05 pages:1228-1235 https://doi.org/10.1007/s10909-018-1938-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY GBV_ILN_22 GBV_ILN_70 GBV_ILN_170 GBV_ILN_4126 AR 193 2018 5-6 10 05 1228-1235 |
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10.1007/s10909-018-1938-8 doi (DE-627)OLC2036831931 (DE-He213)s10909-018-1938-8-p DE-627 ger DE-627 rakwb eng 530 VZ Fukuda, D. verfasserin aut Confocal Microscopy Imaging with an Optical Transition Edge Sensor 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below $$1\,\mu \hbox {W}$$. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate ($$<\,1$$ Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes. Fluorescence Photobleaching Dark count Detection efficiency Resolution Niwa, K. aut Hattori, K. aut Inoue, S. aut Kobayashi, R. aut Numata, T. aut Enthalten in Journal of low temperature physics Springer US, 1969 193(2018), 5-6 vom: 10. Mai, Seite 1228-1235 (DE-627)129546267 (DE-600)218311-0 (DE-576)014996642 0022-2291 nnns volume:193 year:2018 number:5-6 day:10 month:05 pages:1228-1235 https://doi.org/10.1007/s10909-018-1938-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY GBV_ILN_22 GBV_ILN_70 GBV_ILN_170 GBV_ILN_4126 AR 193 2018 5-6 10 05 1228-1235 |
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10.1007/s10909-018-1938-8 doi (DE-627)OLC2036831931 (DE-He213)s10909-018-1938-8-p DE-627 ger DE-627 rakwb eng 530 VZ Fukuda, D. verfasserin aut Confocal Microscopy Imaging with an Optical Transition Edge Sensor 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below $$1\,\mu \hbox {W}$$. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate ($$<\,1$$ Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes. Fluorescence Photobleaching Dark count Detection efficiency Resolution Niwa, K. aut Hattori, K. aut Inoue, S. aut Kobayashi, R. aut Numata, T. aut Enthalten in Journal of low temperature physics Springer US, 1969 193(2018), 5-6 vom: 10. Mai, Seite 1228-1235 (DE-627)129546267 (DE-600)218311-0 (DE-576)014996642 0022-2291 nnns volume:193 year:2018 number:5-6 day:10 month:05 pages:1228-1235 https://doi.org/10.1007/s10909-018-1938-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY GBV_ILN_22 GBV_ILN_70 GBV_ILN_170 GBV_ILN_4126 AR 193 2018 5-6 10 05 1228-1235 |
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10.1007/s10909-018-1938-8 doi (DE-627)OLC2036831931 (DE-He213)s10909-018-1938-8-p DE-627 ger DE-627 rakwb eng 530 VZ Fukuda, D. verfasserin aut Confocal Microscopy Imaging with an Optical Transition Edge Sensor 2018 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below $$1\,\mu \hbox {W}$$. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate ($$<\,1$$ Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes. Fluorescence Photobleaching Dark count Detection efficiency Resolution Niwa, K. aut Hattori, K. aut Inoue, S. aut Kobayashi, R. aut Numata, T. aut Enthalten in Journal of low temperature physics Springer US, 1969 193(2018), 5-6 vom: 10. Mai, Seite 1228-1235 (DE-627)129546267 (DE-600)218311-0 (DE-576)014996642 0022-2291 nnns volume:193 year:2018 number:5-6 day:10 month:05 pages:1228-1235 https://doi.org/10.1007/s10909-018-1938-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY GBV_ILN_22 GBV_ILN_70 GBV_ILN_170 GBV_ILN_4126 AR 193 2018 5-6 10 05 1228-1235 |
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Journal of low temperature physics |
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Confocal Microscopy Imaging with an Optical Transition Edge Sensor |
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Confocal Microscopy Imaging with an Optical Transition Edge Sensor |
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Fukuda, D. |
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Journal of low temperature physics |
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Fukuda, D. Niwa, K. Hattori, K. Inoue, S. Kobayashi, R. Numata, T. |
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confocal microscopy imaging with an optical transition edge sensor |
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Confocal Microscopy Imaging with an Optical Transition Edge Sensor |
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Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below $$1\,\mu \hbox {W}$$. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate ($$<\,1$$ Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes. © Springer Science+Business Media, LLC, part of Springer Nature 2018 |
| abstractGer |
Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below $$1\,\mu \hbox {W}$$. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate ($$<\,1$$ Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes. © Springer Science+Business Media, LLC, part of Springer Nature 2018 |
| abstract_unstemmed |
Abstract Fluorescence color imaging at an extremely low excitation intensity was performed using an optical transition edge sensor (TES) embedded in a confocal microscope for the first time. Optical TES has the ability to resolve incident single photon energy; therefore, the wavelength of each photon can be measured without spectroscopic elements such as diffraction gratings. As target objects, animal cells labeled with two fluorescent dyes were irradiated with an excitation laser at an intensity below $$1\,\mu \hbox {W}$$. In our confocal system, an optical fiber-coupled TES device is used to detect photons instead of the pinhole and photomultiplier tube used in typical confocal microscopes. Photons emitted from the dyes were collected by the objective lens, and sent to the optical TES via the fiber. The TES measures the wavelength of each photon arriving in an exposure time of 70 ms, and a fluorescent photon spectrum is constructed. This measurement is repeated by scanning the target sample, and finally a two-dimensional RGB-color image is obtained. The obtained image showed that the photons emitted from the dyes of mitochondria and cytoskeletons were clearly resolved at a detection intensity level of tens of photons. TES exhibits ideal performance as a photon detector with a low dark count rate ($$<\,1$$ Hz) and wavelength resolving power. In the single-mode fiber-coupled system, the confocal microscope can be operated in the super-resolution mode. These features are very promising to realize high-sensitivity and high-resolution photon spectral imaging, and would help avoid cell damage and photobleaching of fluorescence dyes. © Springer Science+Business Media, LLC, part of Springer Nature 2018 |
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Confocal Microscopy Imaging with an Optical Transition Edge Sensor |
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