Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature
Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band,...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Raitsimring, A. [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2012 |
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| Schlagwörter: |
Electron Paramagnetic Resonance |
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| Systematik: |
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| Anmerkung: |
© Springer-Verlag Wien 2012 |
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| Übergeordnetes Werk: |
Enthalten in: Applied magnetic resonance - Springer Vienna, 1990, 44(2012), 6 vom: 29. Dez., Seite 649-670 |
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| Übergeordnetes Werk: |
volume:44 ; year:2012 ; number:6 ; day:29 ; month:12 ; pages:649-670 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00723-012-0434-6 |
|---|
| Katalog-ID: |
OLC2043925644 |
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| 520 | |a Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. | ||
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10.1007/s00723-012-0434-6 doi (DE-627)OLC2043925644 (DE-He213)s00723-012-0434-6-p DE-627 ger DE-627 rakwb eng 530 620 VZ UA 2242.9 VZ rvk 53.00 bkl 33.00 bkl Raitsimring, A. verfasserin aut Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature 2012 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Wien 2012 Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Electron Spin Echo Lattice Relaxation Rate Supplementary Material Astashkin, A. V. aut Enemark, J. H. aut Kaminker, I. aut Goldfarb, D. aut Walter, E. D. aut Song, Y. aut Meade, T. J. aut Enthalten in Applied magnetic resonance Springer Vienna, 1990 44(2012), 6 vom: 29. Dez., Seite 649-670 (DE-627)13092427X (DE-600)1054553-0 (DE-576)02803757X 0937-9347 nnns volume:44 year:2012 number:6 day:29 month:12 pages:649-670 https://doi.org/10.1007/s00723-012-0434-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY GBV_ILN_65 GBV_ILN_70 GBV_ILN_2010 UA 2242.9 53.00 VZ 33.00 VZ AR 44 2012 6 29 12 649-670 |
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10.1007/s00723-012-0434-6 doi (DE-627)OLC2043925644 (DE-He213)s00723-012-0434-6-p DE-627 ger DE-627 rakwb eng 530 620 VZ UA 2242.9 VZ rvk 53.00 bkl 33.00 bkl Raitsimring, A. verfasserin aut Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature 2012 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Wien 2012 Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Electron Spin Echo Lattice Relaxation Rate Supplementary Material Astashkin, A. V. aut Enemark, J. H. aut Kaminker, I. aut Goldfarb, D. aut Walter, E. D. aut Song, Y. aut Meade, T. J. aut Enthalten in Applied magnetic resonance Springer Vienna, 1990 44(2012), 6 vom: 29. Dez., Seite 649-670 (DE-627)13092427X (DE-600)1054553-0 (DE-576)02803757X 0937-9347 nnns volume:44 year:2012 number:6 day:29 month:12 pages:649-670 https://doi.org/10.1007/s00723-012-0434-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY GBV_ILN_65 GBV_ILN_70 GBV_ILN_2010 UA 2242.9 53.00 VZ 33.00 VZ AR 44 2012 6 29 12 649-670 |
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10.1007/s00723-012-0434-6 doi (DE-627)OLC2043925644 (DE-He213)s00723-012-0434-6-p DE-627 ger DE-627 rakwb eng 530 620 VZ UA 2242.9 VZ rvk 53.00 bkl 33.00 bkl Raitsimring, A. verfasserin aut Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature 2012 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Wien 2012 Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Electron Spin Echo Lattice Relaxation Rate Supplementary Material Astashkin, A. V. aut Enemark, J. H. aut Kaminker, I. aut Goldfarb, D. aut Walter, E. D. aut Song, Y. aut Meade, T. J. aut Enthalten in Applied magnetic resonance Springer Vienna, 1990 44(2012), 6 vom: 29. Dez., Seite 649-670 (DE-627)13092427X (DE-600)1054553-0 (DE-576)02803757X 0937-9347 nnns volume:44 year:2012 number:6 day:29 month:12 pages:649-670 https://doi.org/10.1007/s00723-012-0434-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY GBV_ILN_65 GBV_ILN_70 GBV_ILN_2010 UA 2242.9 53.00 VZ 33.00 VZ AR 44 2012 6 29 12 649-670 |
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10.1007/s00723-012-0434-6 doi (DE-627)OLC2043925644 (DE-He213)s00723-012-0434-6-p DE-627 ger DE-627 rakwb eng 530 620 VZ UA 2242.9 VZ rvk 53.00 bkl 33.00 bkl Raitsimring, A. verfasserin aut Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature 2012 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Wien 2012 Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Electron Spin Echo Lattice Relaxation Rate Supplementary Material Astashkin, A. V. aut Enemark, J. H. aut Kaminker, I. aut Goldfarb, D. aut Walter, E. D. aut Song, Y. aut Meade, T. J. aut Enthalten in Applied magnetic resonance Springer Vienna, 1990 44(2012), 6 vom: 29. Dez., Seite 649-670 (DE-627)13092427X (DE-600)1054553-0 (DE-576)02803757X 0937-9347 nnns volume:44 year:2012 number:6 day:29 month:12 pages:649-670 https://doi.org/10.1007/s00723-012-0434-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY GBV_ILN_65 GBV_ILN_70 GBV_ILN_2010 UA 2242.9 53.00 VZ 33.00 VZ AR 44 2012 6 29 12 649-670 |
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10.1007/s00723-012-0434-6 doi (DE-627)OLC2043925644 (DE-He213)s00723-012-0434-6-p DE-627 ger DE-627 rakwb eng 530 620 VZ UA 2242.9 VZ rvk 53.00 bkl 33.00 bkl Raitsimring, A. verfasserin aut Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature 2012 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Wien 2012 Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Electron Spin Echo Lattice Relaxation Rate Supplementary Material Astashkin, A. V. aut Enemark, J. H. aut Kaminker, I. aut Goldfarb, D. aut Walter, E. D. aut Song, Y. aut Meade, T. J. aut Enthalten in Applied magnetic resonance Springer Vienna, 1990 44(2012), 6 vom: 29. Dez., Seite 649-670 (DE-627)13092427X (DE-600)1054553-0 (DE-576)02803757X 0937-9347 nnns volume:44 year:2012 number:6 day:29 month:12 pages:649-670 https://doi.org/10.1007/s00723-012-0434-6 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHY GBV_ILN_65 GBV_ILN_70 GBV_ILN_2010 UA 2242.9 53.00 VZ 33.00 VZ AR 44 2012 6 29 12 649-670 |
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530 620 VZ UA 2242.9 VZ rvk 53.00 bkl 33.00 bkl Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature Electron Paramagnetic Resonance Electron Paramagnetic Resonance Spectrum Electron Spin Echo Lattice Relaxation Rate Supplementary Material |
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Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature |
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Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature |
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Raitsimring, A. |
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Raitsimring, A. Astashkin, A. V. Enemark, J. H. Kaminker, I. Goldfarb, D. Walter, E. D. Song, Y. Meade, T. J. |
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optimization of pulsed-deer measurements for gd-based labels: choice of operational frequencies, pulse durations and positions, and temperature |
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Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature |
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Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. © Springer-Verlag Wien 2012 |
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Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. © Springer-Verlag Wien 2012 |
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Abstract In this work, the experimental conditions and parameters necessary to optimize the long-distance (≥60 Å) double electron–electron resonance (DEER) measurements of biomacromolecules labeled with Gd(III) tags are analyzed. The specific parameters discussed are the temperature, microwave band, the separation between the pumping and observation frequencies, pulse train repetition rate, pulse durations and pulse positioning in the electron paramagnetic resonance spectrum. It was found that: (1) in optimized DEER measurements, the observation pulses have to be applied at the maximum of the electron paramagnetic resonance spectrum; (2) the optimal temperature range for $ K_{a} $-band measurements is 14–17 K, while in W-band the optimal temperatures are between 6 and 9 K; (iv) W-band is preferable to $ K_{a} $-band for DEER measurements. Recent achievements and the conditions necessary for short-distance measurements (<15 Å) are also briefly discussed. © Springer-Verlag Wien 2012 |
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Optimization of Pulsed-DEER Measurements for Gd-Based Labels: Choice of Operational Frequencies, Pulse Durations and Positions, and Temperature |
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