Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs)
Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly impr...
Ausführliche Beschreibung
Autor*in: |
Hawkins, Daniel M. [verfasserIn] |
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Format: |
Artikel |
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Sprache: |
Englisch |
Erschienen: |
2007 |
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Schlagwörter: |
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Anmerkung: |
© Springer Science+Business Media, LLC 2007 |
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Übergeordnetes Werk: |
Enthalten in: Journal of materials science - Springer US, 1966, 42(2007), 22 vom: 01. Nov., Seite 9465-9468 |
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Übergeordnetes Werk: |
volume:42 ; year:2007 ; number:22 ; day:01 ; month:11 ; pages:9465-9468 |
Links: |
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DOI / URN: |
10.1007/s10853-007-1806-4 |
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Katalog-ID: |
OLC2046333039 |
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10.1007/s10853-007-1806-4 doi (DE-627)OLC2046333039 (DE-He213)s10853-007-1806-4-p DE-627 ger DE-627 rakwb eng 670 VZ Hawkins, Daniel M. verfasserin aut Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2007 Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. Molecularly Imprint Polymer Imprint Polymer Template Molecule Sample Preparation Technique Template Protein Ellis, E. Ann aut Stevenson, Derek aut Holzenburg, Andreas aut Reddy, Subrayal M. aut Enthalten in Journal of materials science Springer US, 1966 42(2007), 22 vom: 01. Nov., Seite 9465-9468 (DE-627)129546372 (DE-600)218324-9 (DE-576)014996774 0022-2461 nnns volume:42 year:2007 number:22 day:01 month:11 pages:9465-9468 https://doi.org/10.1007/s10853-007-1806-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC GBV_ILN_20 GBV_ILN_21 GBV_ILN_23 GBV_ILN_30 GBV_ILN_32 GBV_ILN_40 GBV_ILN_62 GBV_ILN_65 GBV_ILN_70 GBV_ILN_100 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_4046 GBV_ILN_4305 GBV_ILN_4323 AR 42 2007 22 01 11 9465-9468 |
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10.1007/s10853-007-1806-4 doi (DE-627)OLC2046333039 (DE-He213)s10853-007-1806-4-p DE-627 ger DE-627 rakwb eng 670 VZ Hawkins, Daniel M. verfasserin aut Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2007 Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. Molecularly Imprint Polymer Imprint Polymer Template Molecule Sample Preparation Technique Template Protein Ellis, E. Ann aut Stevenson, Derek aut Holzenburg, Andreas aut Reddy, Subrayal M. aut Enthalten in Journal of materials science Springer US, 1966 42(2007), 22 vom: 01. Nov., Seite 9465-9468 (DE-627)129546372 (DE-600)218324-9 (DE-576)014996774 0022-2461 nnns volume:42 year:2007 number:22 day:01 month:11 pages:9465-9468 https://doi.org/10.1007/s10853-007-1806-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC GBV_ILN_20 GBV_ILN_21 GBV_ILN_23 GBV_ILN_30 GBV_ILN_32 GBV_ILN_40 GBV_ILN_62 GBV_ILN_65 GBV_ILN_70 GBV_ILN_100 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_4046 GBV_ILN_4305 GBV_ILN_4323 AR 42 2007 22 01 11 9465-9468 |
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10.1007/s10853-007-1806-4 doi (DE-627)OLC2046333039 (DE-He213)s10853-007-1806-4-p DE-627 ger DE-627 rakwb eng 670 VZ Hawkins, Daniel M. verfasserin aut Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2007 Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. Molecularly Imprint Polymer Imprint Polymer Template Molecule Sample Preparation Technique Template Protein Ellis, E. Ann aut Stevenson, Derek aut Holzenburg, Andreas aut Reddy, Subrayal M. aut Enthalten in Journal of materials science Springer US, 1966 42(2007), 22 vom: 01. Nov., Seite 9465-9468 (DE-627)129546372 (DE-600)218324-9 (DE-576)014996774 0022-2461 nnns volume:42 year:2007 number:22 day:01 month:11 pages:9465-9468 https://doi.org/10.1007/s10853-007-1806-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC GBV_ILN_20 GBV_ILN_21 GBV_ILN_23 GBV_ILN_30 GBV_ILN_32 GBV_ILN_40 GBV_ILN_62 GBV_ILN_65 GBV_ILN_70 GBV_ILN_100 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_4046 GBV_ILN_4305 GBV_ILN_4323 AR 42 2007 22 01 11 9465-9468 |
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10.1007/s10853-007-1806-4 doi (DE-627)OLC2046333039 (DE-He213)s10853-007-1806-4-p DE-627 ger DE-627 rakwb eng 670 VZ Hawkins, Daniel M. verfasserin aut Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2007 Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. Molecularly Imprint Polymer Imprint Polymer Template Molecule Sample Preparation Technique Template Protein Ellis, E. Ann aut Stevenson, Derek aut Holzenburg, Andreas aut Reddy, Subrayal M. aut Enthalten in Journal of materials science Springer US, 1966 42(2007), 22 vom: 01. Nov., Seite 9465-9468 (DE-627)129546372 (DE-600)218324-9 (DE-576)014996774 0022-2461 nnns volume:42 year:2007 number:22 day:01 month:11 pages:9465-9468 https://doi.org/10.1007/s10853-007-1806-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC GBV_ILN_20 GBV_ILN_21 GBV_ILN_23 GBV_ILN_30 GBV_ILN_32 GBV_ILN_40 GBV_ILN_62 GBV_ILN_65 GBV_ILN_70 GBV_ILN_100 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_4046 GBV_ILN_4305 GBV_ILN_4323 AR 42 2007 22 01 11 9465-9468 |
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10.1007/s10853-007-1806-4 doi (DE-627)OLC2046333039 (DE-He213)s10853-007-1806-4-p DE-627 ger DE-627 rakwb eng 670 VZ Hawkins, Daniel M. verfasserin aut Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2007 Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. Molecularly Imprint Polymer Imprint Polymer Template Molecule Sample Preparation Technique Template Protein Ellis, E. Ann aut Stevenson, Derek aut Holzenburg, Andreas aut Reddy, Subrayal M. aut Enthalten in Journal of materials science Springer US, 1966 42(2007), 22 vom: 01. Nov., Seite 9465-9468 (DE-627)129546372 (DE-600)218324-9 (DE-576)014996774 0022-2461 nnns volume:42 year:2007 number:22 day:01 month:11 pages:9465-9468 https://doi.org/10.1007/s10853-007-1806-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC GBV_ILN_20 GBV_ILN_21 GBV_ILN_23 GBV_ILN_30 GBV_ILN_32 GBV_ILN_40 GBV_ILN_62 GBV_ILN_65 GBV_ILN_70 GBV_ILN_100 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_4046 GBV_ILN_4305 GBV_ILN_4323 AR 42 2007 22 01 11 9465-9468 |
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670 VZ Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) Molecularly Imprint Polymer Imprint Polymer Template Molecule Sample Preparation Technique Template Protein |
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Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) |
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Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) |
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Hawkins, Daniel M. |
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Journal of materials science |
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Hawkins, Daniel M. Ellis, E. Ann Stevenson, Derek Holzenburg, Andreas Reddy, Subrayal M. |
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670 |
title_sort |
novel critical point drying (cpd) based preparation and transmission electron microscopy (tem) imaging of protein specific molecularly imprinted polymers (hydromips) |
title_auth |
Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) |
abstract |
Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. © Springer Science+Business Media, LLC 2007 |
abstractGer |
Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. © Springer Science+Business Media, LLC 2007 |
abstract_unstemmed |
Abstract We report the transmission electron microscopy (TEM) imaging of a hydrogel based molecularly imprinted polymer (HydroMIP) specific to the template molecule bovine haemoglobin (BHb). A novel critical point drying based sample preparation technique was employed to prepare the molecularly imprinted polymer (MIP) samples in a manner that would facilitate the use of TEM to image the imprinted cavities, and provide an appropriate degree of both magnification and resolution to image polymer architecture in the <10 nm range. For the first time, polymer structure has been detailed that tentatively suggests molecularly imprinted cavities, ranging from 5 to 50 nm in size, that correlate (in terms of size) with the protein molecule employed as the imprinting template. The modified critical point drying sample preparation technique used may potentially play a key role in the imaging of all molecularly imprinted polymers, particularly those prepared in the aqueous phase. © Springer Science+Business Media, LLC 2007 |
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title_short |
Novel critical point drying (CPD) based preparation and transmission electron microscopy (TEM) imaging of protein specific molecularly imprinted polymers (HydroMIPs) |
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