Utilization of whey fractions as a substitute for fetal calf serum in culture media
Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferatio...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Damerdji, O. [verfasserIn] |
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| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
1988 |
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| Schlagwörter: |
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| Anmerkung: |
© Science & Technology Letters 1988 |
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| Übergeordnetes Werk: |
Enthalten in: Biotechnology techniques - Kluwer Academic Publishers, 1987, 2(1988), 4 vom: Dez., Seite 253-258 |
|---|---|
| Übergeordnetes Werk: |
volume:2 ; year:1988 ; number:4 ; month:12 ; pages:253-258 |
| Links: |
|---|
| DOI / URN: |
10.1007/BF01875538 |
|---|
| Katalog-ID: |
OLC2050585845 |
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| 520 | |a Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. | ||
| 650 | 4 | |a Fetal Calf Serum | |
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| 650 | 4 | |a Cell Line Culture | |
| 650 | 4 | |a Line Culture | |
| 650 | 4 | |a Whey Fraction | |
| 700 | 1 | |a Derouiche, F. |4 aut | |
| 700 | 1 | |a Legrand, C. |4 aut | |
| 700 | 1 | |a Capiaumont, J. |4 aut | |
| 700 | 1 | |a Bour, J. M. |4 aut | |
| 700 | 1 | |a Maugras, M. |4 aut | |
| 700 | 1 | |a Belleville, F. |4 aut | |
| 700 | 1 | |a Nabet, P. |4 aut | |
| 700 | 1 | |a Paquet, D. |4 aut | |
| 700 | 1 | |a Linden, G. |4 aut | |
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10.1007/BF01875538 doi (DE-627)OLC2050585845 (DE-He213)BF01875538-p DE-627 ger DE-627 rakwb eng 570 660 VZ 12 ssgn Damerdji, O. verfasserin aut Utilization of whey fractions as a substitute for fetal calf serum in culture media 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Science & Technology Letters 1988 Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. Fetal Calf Serum Calf Serum Cell Line Culture Line Culture Whey Fraction Derouiche, F. aut Legrand, C. aut Capiaumont, J. aut Bour, J. M. aut Maugras, M. aut Belleville, F. aut Nabet, P. aut Paquet, D. aut Linden, G. aut Enthalten in Biotechnology techniques Kluwer Academic Publishers, 1987 2(1988), 4 vom: Dez., Seite 253-258 (DE-627)170325377 (DE-600)1041757-6 (DE-576)023129352 0951-208X nnns volume:2 year:1988 number:4 month:12 pages:253-258 https://doi.org/10.1007/BF01875538 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_2015 GBV_ILN_4012 GBV_ILN_4219 AR 2 1988 4 12 253-258 |
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10.1007/BF01875538 doi (DE-627)OLC2050585845 (DE-He213)BF01875538-p DE-627 ger DE-627 rakwb eng 570 660 VZ 12 ssgn Damerdji, O. verfasserin aut Utilization of whey fractions as a substitute for fetal calf serum in culture media 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Science & Technology Letters 1988 Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. Fetal Calf Serum Calf Serum Cell Line Culture Line Culture Whey Fraction Derouiche, F. aut Legrand, C. aut Capiaumont, J. aut Bour, J. M. aut Maugras, M. aut Belleville, F. aut Nabet, P. aut Paquet, D. aut Linden, G. aut Enthalten in Biotechnology techniques Kluwer Academic Publishers, 1987 2(1988), 4 vom: Dez., Seite 253-258 (DE-627)170325377 (DE-600)1041757-6 (DE-576)023129352 0951-208X nnns volume:2 year:1988 number:4 month:12 pages:253-258 https://doi.org/10.1007/BF01875538 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_2015 GBV_ILN_4012 GBV_ILN_4219 AR 2 1988 4 12 253-258 |
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10.1007/BF01875538 doi (DE-627)OLC2050585845 (DE-He213)BF01875538-p DE-627 ger DE-627 rakwb eng 570 660 VZ 12 ssgn Damerdji, O. verfasserin aut Utilization of whey fractions as a substitute for fetal calf serum in culture media 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Science & Technology Letters 1988 Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. Fetal Calf Serum Calf Serum Cell Line Culture Line Culture Whey Fraction Derouiche, F. aut Legrand, C. aut Capiaumont, J. aut Bour, J. M. aut Maugras, M. aut Belleville, F. aut Nabet, P. aut Paquet, D. aut Linden, G. aut Enthalten in Biotechnology techniques Kluwer Academic Publishers, 1987 2(1988), 4 vom: Dez., Seite 253-258 (DE-627)170325377 (DE-600)1041757-6 (DE-576)023129352 0951-208X nnns volume:2 year:1988 number:4 month:12 pages:253-258 https://doi.org/10.1007/BF01875538 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_2015 GBV_ILN_4012 GBV_ILN_4219 AR 2 1988 4 12 253-258 |
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10.1007/BF01875538 doi (DE-627)OLC2050585845 (DE-He213)BF01875538-p DE-627 ger DE-627 rakwb eng 570 660 VZ 12 ssgn Damerdji, O. verfasserin aut Utilization of whey fractions as a substitute for fetal calf serum in culture media 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Science & Technology Letters 1988 Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. Fetal Calf Serum Calf Serum Cell Line Culture Line Culture Whey Fraction Derouiche, F. aut Legrand, C. aut Capiaumont, J. aut Bour, J. M. aut Maugras, M. aut Belleville, F. aut Nabet, P. aut Paquet, D. aut Linden, G. aut Enthalten in Biotechnology techniques Kluwer Academic Publishers, 1987 2(1988), 4 vom: Dez., Seite 253-258 (DE-627)170325377 (DE-600)1041757-6 (DE-576)023129352 0951-208X nnns volume:2 year:1988 number:4 month:12 pages:253-258 https://doi.org/10.1007/BF01875538 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_2015 GBV_ILN_4012 GBV_ILN_4219 AR 2 1988 4 12 253-258 |
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10.1007/BF01875538 doi (DE-627)OLC2050585845 (DE-He213)BF01875538-p DE-627 ger DE-627 rakwb eng 570 660 VZ 12 ssgn Damerdji, O. verfasserin aut Utilization of whey fractions as a substitute for fetal calf serum in culture media 1988 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Science & Technology Letters 1988 Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. Fetal Calf Serum Calf Serum Cell Line Culture Line Culture Whey Fraction Derouiche, F. aut Legrand, C. aut Capiaumont, J. aut Bour, J. M. aut Maugras, M. aut Belleville, F. aut Nabet, P. aut Paquet, D. aut Linden, G. aut Enthalten in Biotechnology techniques Kluwer Academic Publishers, 1987 2(1988), 4 vom: Dez., Seite 253-258 (DE-627)170325377 (DE-600)1041757-6 (DE-576)023129352 0951-208X nnns volume:2 year:1988 number:4 month:12 pages:253-258 https://doi.org/10.1007/BF01875538 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_2015 GBV_ILN_4012 GBV_ILN_4219 AR 2 1988 4 12 253-258 |
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utilization of whey fractions as a substitute for fetal calf serum in culture media |
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Utilization of whey fractions as a substitute for fetal calf serum in culture media |
| abstract |
Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. © Science & Technology Letters 1988 |
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Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. © Science & Technology Letters 1988 |
| abstract_unstemmed |
Summary A dairy by-product was used in cell line cultures as a replacement for fetal calf serum (FCS). Various fractions were prepared from whey. All fractions have the ability to stimulate DNA synthesis in human and mouse 3T3 fibroblast and hybridoma cultures. They also allow hybridoma proliferation in the long term using only 0.5% FCS. In these latter experiments, heated and ultrafiltered fractions were more effective than 10% fetal calf serum. © Science & Technology Letters 1988 |
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Utilization of whey fractions as a substitute for fetal calf serum in culture media |
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Derouiche, F. Legrand, C. Capiaumont, J. Bour, J. M. Maugras, M. Belleville, F. Nabet, P. Paquet, D. Linden, G. |
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Derouiche, F. Legrand, C. Capiaumont, J. Bour, J. M. Maugras, M. Belleville, F. Nabet, P. Paquet, D. Linden, G. |
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10.1007/BF01875538 |
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