Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae

Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a se...
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Autor*in:	Tsuchiya, Kozo [verfasserIn] Gomi, Katsuya Kitamoto, Katsuhiko Kumagai, Chieko Tamura, Gakuzo

Format:	Artikel
Sprache:	Englisch

Erschienen:	1993

Schlagwörter:	Western Blot Blot Analysis Western Blot Analysis Aspergillus Northern Blot

Anmerkung:	© Springer-Verlag 1993

Übergeordnetes Werk:	Enthalten in: Applied microbiology and biotechnology - Springer-Verlag, 1984, 40(1993), 2-3 vom: Nov., Seite 327-332
Übergeordnetes Werk:	volume:40 ; year:1993 ; number:2-3 ; month:11 ; pages:327-332

Links:	Volltext

DOI / URN:	10.1007/BF00170388

Katalog-ID:	OLC2050670176

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520			\|a Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome.
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allfields	10.1007/BF00170388 doi (DE-627)OLC2050670176 (DE-He213)BF00170388-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Tsuchiya, Kozo verfasserin aut Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae 1993 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1993 Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome. Western Blot Blot Analysis Western Blot Analysis Aspergillus Northern Blot Gomi, Katsuya aut Kitamoto, Katsuhiko aut Kumagai, Chieko aut Tamura, Gakuzo aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 40(1993), 2-3 vom: Nov., Seite 327-332 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:40 year:1993 number:2-3 month:11 pages:327-332 https://doi.org/10.1007/BF00170388 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 40 1993 2-3 11 327-332
spelling	10.1007/BF00170388 doi (DE-627)OLC2050670176 (DE-He213)BF00170388-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Tsuchiya, Kozo verfasserin aut Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae 1993 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1993 Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome. Western Blot Blot Analysis Western Blot Analysis Aspergillus Northern Blot Gomi, Katsuya aut Kitamoto, Katsuhiko aut Kumagai, Chieko aut Tamura, Gakuzo aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 40(1993), 2-3 vom: Nov., Seite 327-332 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:40 year:1993 number:2-3 month:11 pages:327-332 https://doi.org/10.1007/BF00170388 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 40 1993 2-3 11 327-332
allfields_unstemmed	10.1007/BF00170388 doi (DE-627)OLC2050670176 (DE-He213)BF00170388-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Tsuchiya, Kozo verfasserin aut Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae 1993 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1993 Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome. Western Blot Blot Analysis Western Blot Analysis Aspergillus Northern Blot Gomi, Katsuya aut Kitamoto, Katsuhiko aut Kumagai, Chieko aut Tamura, Gakuzo aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 40(1993), 2-3 vom: Nov., Seite 327-332 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:40 year:1993 number:2-3 month:11 pages:327-332 https://doi.org/10.1007/BF00170388 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 40 1993 2-3 11 327-332
allfieldsGer	10.1007/BF00170388 doi (DE-627)OLC2050670176 (DE-He213)BF00170388-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Tsuchiya, Kozo verfasserin aut Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae 1993 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1993 Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome. Western Blot Blot Analysis Western Blot Analysis Aspergillus Northern Blot Gomi, Katsuya aut Kitamoto, Katsuhiko aut Kumagai, Chieko aut Tamura, Gakuzo aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 40(1993), 2-3 vom: Nov., Seite 327-332 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:40 year:1993 number:2-3 month:11 pages:327-332 https://doi.org/10.1007/BF00170388 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 40 1993 2-3 11 327-332
allfieldsSound	10.1007/BF00170388 doi (DE-627)OLC2050670176 (DE-He213)BF00170388-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Tsuchiya, Kozo verfasserin aut Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae 1993 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1993 Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome. Western Blot Blot Analysis Western Blot Analysis Aspergillus Northern Blot Gomi, Katsuya aut Kitamoto, Katsuhiko aut Kumagai, Chieko aut Tamura, Gakuzo aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 40(1993), 2-3 vom: Nov., Seite 327-332 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:40 year:1993 number:2-3 month:11 pages:327-332 https://doi.org/10.1007/BF00170388 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 40 1993 2-3 11 327-332
language	English
source	Enthalten in Applied microbiology and biotechnology 40(1993), 2-3 vom: Nov., Seite 327-332 volume:40 year:1993 number:2-3 month:11 pages:327-332
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topic_facet	Western Blot Blot Analysis Western Blot Analysis Aspergillus Northern Blot
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author	Tsuchiya, Kozo
spellingShingle	Tsuchiya, Kozo ddc 570 ssgn 12 fid BIODIV misc Western Blot misc Blot Analysis misc Western Blot Analysis misc Aspergillus misc Northern Blot Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae
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topic_title	570 VZ 12 ssgn BIODIV DE-30 fid Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae Western Blot Blot Analysis Western Blot Analysis Aspergillus Northern Blot
topic	ddc 570 ssgn 12 fid BIODIV misc Western Blot misc Blot Analysis misc Western Blot Analysis misc Aspergillus misc Northern Blot
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title	Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae
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title_full	Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae
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author_browse	Tsuchiya, Kozo Gomi, Katsuya Kitamoto, Katsuhiko Kumagai, Chieko Tamura, Gakuzo
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title_sort	secretion of calf chymosin from the filamentous fungus aspergillus oryzae
title_auth	Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae
abstract	Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome. © Springer-Verlag 1993
abstractGer	Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome. © Springer-Verlag 1993
abstract_unstemmed	Abstract Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07– 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome. © Springer-Verlag 1993
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title_short	Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae
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author2	Gomi, Katsuya Kitamoto, Katsuhiko Kumagai, Chieko Tamura, Gakuzo
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up_date	2024-07-04T02:32:26.759Z
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