Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system

Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Kawakubo, Y. [verfasserIn] Matsushita, T. Funatsu, K.

Format:	Artikel
Sprache:	Englisch

Erschienen:	1994

Schlagwörter:	Foam Cell Growth Stationary Culture Stationary Phase Cell Density

Anmerkung:	© Springer-Verlag 1994

Übergeordnetes Werk:	Enthalten in: Applied microbiology and biotechnology - Springer-Verlag, 1984, 41(1994), 4 vom: Juni, Seite 413-418
Übergeordnetes Werk:	volume:41 ; year:1994 ; number:4 ; month:06 ; pages:413-418

Links:	Volltext

DOI / URN:	10.1007/BF00939029

Katalog-ID:	OLC2050672055

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allfields	10.1007/BF00939029 doi (DE-627)OLC2050672055 (DE-He213)BF00939029-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Kawakubo, Y. verfasserin aut Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system 1994 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1994 Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system for high density culture and continuous production of t-PA. A maximal cell density of 3.24 × $ 10^{7} $ cells/$ cm^{3} $ PUF and a t-PA productivity of 0.326 IU/$ 10^{6} $cells per day were obtained in 25-day perfusion cultures. Although the cell density decreased to half that in PUF stationary culture, the t-PA productivity increased twofold and was maintained for 25 culture days. Foam Cell Growth Stationary Culture Stationary Phase Cell Density Matsushita, T. aut Funatsu, K. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 41(1994), 4 vom: Juni, Seite 413-418 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:41 year:1994 number:4 month:06 pages:413-418 https://doi.org/10.1007/BF00939029 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 41 1994 4 06 413-418
spelling	10.1007/BF00939029 doi (DE-627)OLC2050672055 (DE-He213)BF00939029-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Kawakubo, Y. verfasserin aut Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system 1994 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1994 Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system for high density culture and continuous production of t-PA. A maximal cell density of 3.24 × $ 10^{7} $ cells/$ cm^{3} $ PUF and a t-PA productivity of 0.326 IU/$ 10^{6} $cells per day were obtained in 25-day perfusion cultures. Although the cell density decreased to half that in PUF stationary culture, the t-PA productivity increased twofold and was maintained for 25 culture days. Foam Cell Growth Stationary Culture Stationary Phase Cell Density Matsushita, T. aut Funatsu, K. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 41(1994), 4 vom: Juni, Seite 413-418 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:41 year:1994 number:4 month:06 pages:413-418 https://doi.org/10.1007/BF00939029 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 41 1994 4 06 413-418
allfields_unstemmed	10.1007/BF00939029 doi (DE-627)OLC2050672055 (DE-He213)BF00939029-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Kawakubo, Y. verfasserin aut Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system 1994 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1994 Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system for high density culture and continuous production of t-PA. A maximal cell density of 3.24 × $ 10^{7} $ cells/$ cm^{3} $ PUF and a t-PA productivity of 0.326 IU/$ 10^{6} $cells per day were obtained in 25-day perfusion cultures. Although the cell density decreased to half that in PUF stationary culture, the t-PA productivity increased twofold and was maintained for 25 culture days. Foam Cell Growth Stationary Culture Stationary Phase Cell Density Matsushita, T. aut Funatsu, K. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 41(1994), 4 vom: Juni, Seite 413-418 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:41 year:1994 number:4 month:06 pages:413-418 https://doi.org/10.1007/BF00939029 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 41 1994 4 06 413-418
allfieldsGer	10.1007/BF00939029 doi (DE-627)OLC2050672055 (DE-He213)BF00939029-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Kawakubo, Y. verfasserin aut Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system 1994 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1994 Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system for high density culture and continuous production of t-PA. A maximal cell density of 3.24 × $ 10^{7} $ cells/$ cm^{3} $ PUF and a t-PA productivity of 0.326 IU/$ 10^{6} $cells per day were obtained in 25-day perfusion cultures. Although the cell density decreased to half that in PUF stationary culture, the t-PA productivity increased twofold and was maintained for 25 culture days. Foam Cell Growth Stationary Culture Stationary Phase Cell Density Matsushita, T. aut Funatsu, K. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 41(1994), 4 vom: Juni, Seite 413-418 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:41 year:1994 number:4 month:06 pages:413-418 https://doi.org/10.1007/BF00939029 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 41 1994 4 06 413-418
allfieldsSound	10.1007/BF00939029 doi (DE-627)OLC2050672055 (DE-He213)BF00939029-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Kawakubo, Y. verfasserin aut Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system 1994 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1994 Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system for high density culture and continuous production of t-PA. A maximal cell density of 3.24 × $ 10^{7} $ cells/$ cm^{3} $ PUF and a t-PA productivity of 0.326 IU/$ 10^{6} $cells per day were obtained in 25-day perfusion cultures. Although the cell density decreased to half that in PUF stationary culture, the t-PA productivity increased twofold and was maintained for 25 culture days. Foam Cell Growth Stationary Culture Stationary Phase Cell Density Matsushita, T. aut Funatsu, K. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 41(1994), 4 vom: Juni, Seite 413-418 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:41 year:1994 number:4 month:06 pages:413-418 https://doi.org/10.1007/BF00939029 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 41 1994 4 06 413-418
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source	Enthalten in Applied microbiology and biotechnology 41(1994), 4 vom: Juni, Seite 413-418 volume:41 year:1994 number:4 month:06 pages:413-418
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author	Kawakubo, Y.
spellingShingle	Kawakubo, Y. ddc 570 ssgn 12 fid BIODIV misc Foam misc Cell Growth misc Stationary Culture misc Stationary Phase misc Cell Density Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system
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topic_title	570 VZ 12 ssgn BIODIV DE-30 fid Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system Foam Cell Growth Stationary Culture Stationary Phase Cell Density
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title	Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system
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title_full	Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system
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title_sort	tissue plasminogen activator (t-pa) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system
title_auth	Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system
abstract	Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system for high density culture and continuous production of t-PA. A maximal cell density of 3.24 × $ 10^{7} $ cells/$ cm^{3} $ PUF and a t-PA productivity of 0.326 IU/$ 10^{6} $cells per day were obtained in 25-day perfusion cultures. Although the cell density decreased to half that in PUF stationary culture, the t-PA productivity increased twofold and was maintained for 25 culture days. © Springer-Verlag 1994
abstractGer	Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system for high density culture and continuous production of t-PA. A maximal cell density of 3.24 × $ 10^{7} $ cells/$ cm^{3} $ PUF and a t-PA productivity of 0.326 IU/$ 10^{6} $cells per day were obtained in 25-day perfusion cultures. Although the cell density decreased to half that in PUF stationary culture, the t-PA productivity increased twofold and was maintained for 25 culture days. © Springer-Verlag 1994
abstract_unstemmed	Abstract Weakly adherent cells of the 293 line attached well to the internal surface of polyurethane foam (PUF) and grew to the high density of 6.83 × $ 10^{7} $ cells/$ cm^{3} $ PUF in stationary culture. The maximum productivity of tissue plasminogen activator (t-PA) was 0.158 IU/$ 10^{6} $ cells per day. The productivity decreased at the stationary phase of cell growth, so we designed a PUF-plate packed-bed culture system for high density culture and continuous production of t-PA. A maximal cell density of 3.24 × $ 10^{7} $ cells/$ cm^{3} $ PUF and a t-PA productivity of 0.326 IU/$ 10^{6} $cells per day were obtained in 25-day perfusion cultures. Although the cell density decreased to half that in PUF stationary culture, the t-PA productivity increased twofold and was maintained for 25 culture days. © Springer-Verlag 1994
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title_short	Tissue plasminogen activator (t-PA) production by a high density culture of weakly adherent human embryonic kidney cells using a polyurethane-foam packed-bed culture system
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