Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells

Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobac...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Cho, H. -J. [verfasserIn] Choi, K. -P. Yamashita, M. Morikawa, H. Murooka, Y.

Format:	Artikel
Sprache:	Englisch

Erschienen:	1995

Schlagwörter:	Streptomyces Northern Blot Mosaic Virus Oxidase Activity Northern Blot Analysis

Anmerkung:	© Springer-Verlag 1995

Übergeordnetes Werk:	Enthalten in: Applied microbiology and biotechnology - Springer-Verlag, 1984, 44(1995), 1-2 vom: Dez., Seite 133-138
Übergeordnetes Werk:	volume:44 ; year:1995 ; number:1-2 ; month:12 ; pages:133-138

Links:	Volltext

DOI / URN:	10.1007/BF00164492

Katalog-ID:	OLC2050676115

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allfields	10.1007/BF00164492 doi (DE-627)OLC2050676115 (DE-He213)BF00164492-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Cho, H. -J. verfasserin aut Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells 1995 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1995 Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line. Streptomyces Northern Blot Mosaic Virus Oxidase Activity Northern Blot Analysis Choi, K. -P. aut Yamashita, M. aut Morikawa, H. aut Murooka, Y. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 44(1995), 1-2 vom: Dez., Seite 133-138 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:44 year:1995 number:1-2 month:12 pages:133-138 https://doi.org/10.1007/BF00164492 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 44 1995 1-2 12 133-138
spelling	10.1007/BF00164492 doi (DE-627)OLC2050676115 (DE-He213)BF00164492-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Cho, H. -J. verfasserin aut Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells 1995 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1995 Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line. Streptomyces Northern Blot Mosaic Virus Oxidase Activity Northern Blot Analysis Choi, K. -P. aut Yamashita, M. aut Morikawa, H. aut Murooka, Y. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 44(1995), 1-2 vom: Dez., Seite 133-138 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:44 year:1995 number:1-2 month:12 pages:133-138 https://doi.org/10.1007/BF00164492 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 44 1995 1-2 12 133-138
allfields_unstemmed	10.1007/BF00164492 doi (DE-627)OLC2050676115 (DE-He213)BF00164492-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Cho, H. -J. verfasserin aut Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells 1995 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1995 Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line. Streptomyces Northern Blot Mosaic Virus Oxidase Activity Northern Blot Analysis Choi, K. -P. aut Yamashita, M. aut Morikawa, H. aut Murooka, Y. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 44(1995), 1-2 vom: Dez., Seite 133-138 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:44 year:1995 number:1-2 month:12 pages:133-138 https://doi.org/10.1007/BF00164492 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 44 1995 1-2 12 133-138
allfieldsGer	10.1007/BF00164492 doi (DE-627)OLC2050676115 (DE-He213)BF00164492-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Cho, H. -J. verfasserin aut Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells 1995 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1995 Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line. Streptomyces Northern Blot Mosaic Virus Oxidase Activity Northern Blot Analysis Choi, K. -P. aut Yamashita, M. aut Morikawa, H. aut Murooka, Y. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 44(1995), 1-2 vom: Dez., Seite 133-138 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:44 year:1995 number:1-2 month:12 pages:133-138 https://doi.org/10.1007/BF00164492 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 44 1995 1-2 12 133-138
allfieldsSound	10.1007/BF00164492 doi (DE-627)OLC2050676115 (DE-He213)BF00164492-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Cho, H. -J. verfasserin aut Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells 1995 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 1995 Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line. Streptomyces Northern Blot Mosaic Virus Oxidase Activity Northern Blot Analysis Choi, K. -P. aut Yamashita, M. aut Morikawa, H. aut Murooka, Y. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 44(1995), 1-2 vom: Dez., Seite 133-138 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:44 year:1995 number:1-2 month:12 pages:133-138 https://doi.org/10.1007/BF00164492 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4103 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 44 1995 1-2 12 133-138
language	English
source	Enthalten in Applied microbiology and biotechnology 44(1995), 1-2 vom: Dez., Seite 133-138 volume:44 year:1995 number:1-2 month:12 pages:133-138
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author	Cho, H. -J.
spellingShingle	Cho, H. -J. ddc 570 ssgn 12 fid BIODIV misc Streptomyces misc Northern Blot misc Mosaic Virus misc Oxidase Activity misc Northern Blot Analysis Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells
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topic_title	570 VZ 12 ssgn BIODIV DE-30 fid Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells Streptomyces Northern Blot Mosaic Virus Oxidase Activity Northern Blot Analysis
topic	ddc 570 ssgn 12 fid BIODIV misc Streptomyces misc Northern Blot misc Mosaic Virus misc Oxidase Activity misc Northern Blot Analysis
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title	Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells
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title_full	Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells
author_sort	Cho, H. -J.
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author_browse	Cho, H. -J. Choi, K. -P. Yamashita, M. Morikawa, H. Murooka, Y.
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title_sort	introduction and expression of the streptomyces cholesterol oxidase gene (choa), a potent insecticidal protein active against boll weevil larvae, into tobacco cells
title_auth	Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells
abstract	Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line. © Springer-Verlag 1995
abstractGer	Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line. © Springer-Verlag 1995
abstract_unstemmed	Abstract We succeeded expressing the streptomyces cholesterol oxidase gene (choA), a potent insecticidal protein active against boll weevil larvae, in tobacco cells. A plasmid, pBC4, containing the choA gene under the control of the 35S promoter of cauliflower mosaic virus, was introduced into tobacco cells (BY-2) using a pneumatic particle gun. Stable integration of the choA gene in the genome of the transformed callus line was confirmed by Southern hybridization analysis. Northern blot analysis revealed material corresponding in size to the choA transcript in the transformed cells. Cholesterol oxidase activity in transformed callus was about eightfold higher than that in control cell line. © Springer-Verlag 1995
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title_short	Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells
url	https://doi.org/10.1007/BF00164492
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author2	Choi, K. -P. Yamashita, M. Morikawa, H. Murooka, Y.
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up_date	2024-07-04T02:33:23.594Z
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