Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4

Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	König, A. [verfasserIn] Zaborosch, C. Muscat, A. Vorlop, K.-D. Spener, F.

Format:	Artikel
Sprache:	Englisch

Erschienen:	1996

Schlagwörter:	Aqueous Solution Detection Limit Response Time Pseudomonas Naphthalene

Anmerkung:	© Springer-Verlag Berlin Heidelberg 1996

Übergeordnetes Werk:	Enthalten in: Applied microbiology and biotechnology - Springer-Verlag, 1984, 45(1996), 6 vom: Juli, Seite 844-850
Übergeordnetes Werk:	volume:45 ; year:1996 ; number:6 ; month:07 ; pages:844-850

Links:	Volltext

DOI / URN:	10.1007/s002530050772

Katalog-ID:	OLC2050678320

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520			\|a Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates.
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allfields	10.1007/s002530050772 doi (DE-627)OLC2050678320 (DE-He213)s002530050772-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid König, A. verfasserin aut Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 1996 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1996 Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates. Aqueous Solution Detection Limit Response Time Pseudomonas Naphthalene Zaborosch, C. aut Muscat, A. aut Vorlop, K.-D. aut Spener, F. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 45(1996), 6 vom: Juli, Seite 844-850 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:45 year:1996 number:6 month:07 pages:844-850 https://doi.org/10.1007/s002530050772 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 45 1996 6 07 844-850
spelling	10.1007/s002530050772 doi (DE-627)OLC2050678320 (DE-He213)s002530050772-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid König, A. verfasserin aut Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 1996 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1996 Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates. Aqueous Solution Detection Limit Response Time Pseudomonas Naphthalene Zaborosch, C. aut Muscat, A. aut Vorlop, K.-D. aut Spener, F. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 45(1996), 6 vom: Juli, Seite 844-850 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:45 year:1996 number:6 month:07 pages:844-850 https://doi.org/10.1007/s002530050772 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 45 1996 6 07 844-850
allfields_unstemmed	10.1007/s002530050772 doi (DE-627)OLC2050678320 (DE-He213)s002530050772-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid König, A. verfasserin aut Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 1996 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1996 Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates. Aqueous Solution Detection Limit Response Time Pseudomonas Naphthalene Zaborosch, C. aut Muscat, A. aut Vorlop, K.-D. aut Spener, F. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 45(1996), 6 vom: Juli, Seite 844-850 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:45 year:1996 number:6 month:07 pages:844-850 https://doi.org/10.1007/s002530050772 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 45 1996 6 07 844-850
allfieldsGer	10.1007/s002530050772 doi (DE-627)OLC2050678320 (DE-He213)s002530050772-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid König, A. verfasserin aut Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 1996 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1996 Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates. Aqueous Solution Detection Limit Response Time Pseudomonas Naphthalene Zaborosch, C. aut Muscat, A. aut Vorlop, K.-D. aut Spener, F. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 45(1996), 6 vom: Juli, Seite 844-850 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:45 year:1996 number:6 month:07 pages:844-850 https://doi.org/10.1007/s002530050772 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 45 1996 6 07 844-850
allfieldsSound	10.1007/s002530050772 doi (DE-627)OLC2050678320 (DE-He213)s002530050772-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid König, A. verfasserin aut Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 1996 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1996 Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates. Aqueous Solution Detection Limit Response Time Pseudomonas Naphthalene Zaborosch, C. aut Muscat, A. aut Vorlop, K.-D. aut Spener, F. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 45(1996), 6 vom: Juli, Seite 844-850 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:45 year:1996 number:6 month:07 pages:844-850 https://doi.org/10.1007/s002530050772 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2006 GBV_ILN_2016 GBV_ILN_2018 GBV_ILN_2021 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4219 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 45 1996 6 07 844-850
language	English
source	Enthalten in Applied microbiology and biotechnology 45(1996), 6 vom: Juli, Seite 844-850 volume:45 year:1996 number:6 month:07 pages:844-850
sourceStr	Enthalten in Applied microbiology and biotechnology 45(1996), 6 vom: Juli, Seite 844-850 volume:45 year:1996 number:6 month:07 pages:844-850
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topic_facet	Aqueous Solution Detection Limit Response Time Pseudomonas Naphthalene
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authorswithroles_txt_mv	König, A. @@aut@@ Zaborosch, C. @@aut@@ Muscat, A. @@aut@@ Vorlop, K.-D. @@aut@@ Spener, F. @@aut@@
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author	König, A.
spellingShingle	König, A. ddc 570 ssgn 12 fid BIODIV misc Aqueous Solution misc Detection Limit misc Response Time misc Pseudomonas misc Naphthalene Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4
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topic_title	570 VZ 12 ssgn BIODIV DE-30 fid Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 Aqueous Solution Detection Limit Response Time Pseudomonas Naphthalene
topic	ddc 570 ssgn 12 fid BIODIV misc Aqueous Solution misc Detection Limit misc Response Time misc Pseudomonas misc Naphthalene
topic_unstemmed	ddc 570 ssgn 12 fid BIODIV misc Aqueous Solution misc Detection Limit misc Response Time misc Pseudomonas misc Naphthalene
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title	Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4
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title_full	Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4
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author_browse	König, A. Zaborosch, C. Muscat, A. Vorlop, K.-D. Spener, F.
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title_sort	microbial sensors for naphthalene using sphingomonas sp. b1 or pseudomonas fluorescens ww4
title_auth	Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4
abstract	Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates. © Springer-Verlag Berlin Heidelberg 1996
abstractGer	Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates. © Springer-Verlag Berlin Heidelberg 1996
abstract_unstemmed	Abstract Amperometric biosensors for naphthalene were developed using either immobilized Sphingomonas sp. B1 or Pseudomonas fluorescens WW4 cells. The microorganisms were immobilized within a polyurethane-based hydrogel, which was used for a microbial biosensor for the first time. Both strains were shown to be equally suited for the quantification of naphthalene in aqueous solutions. The biosensors were tested in a flow-through system and a stirred cell (batch method). In both systems a linear response down to the detection limit was obtained. Measurements in the flow-through system gave sensitivities of up to 1.2 nA $ mg^{−1} $ $ l^{−1} $ and a linear range from 0.03 mg/l to 2.0 mg/l. The response time (t95) was 2 min and the sample throughput six per hour; the repeatability was within ±5 %. With the batch method, sensitivities of between 3 nA $ mg^{−1} $ $ l^{−1} $ and 5 nA $ mg^{−1} $$ l^{−1} $ and a linear range of 0.01–3.0 mg/l were obtained; the response time was between 3 min and 5 min. The sensors reached an operational lifetime of up to 20 days. The sensitivity of both sensors for naphthalene was, in most cases, more than four times higher than for various other substrates. © Springer-Verlag Berlin Heidelberg 1996
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title_short	Microbial sensors for naphthalene using Sphingomonas sp. B1 or Pseudomonas fluorescens WW4
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