Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina
Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed....
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Khashnobish, A. [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1999 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag Berlin Heidelberg 1999 |
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| Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Springer-Verlag, 1984, 52(1999), 2 vom: Aug., Seite 191-195 |
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| Übergeordnetes Werk: |
volume:52 ; year:1999 ; number:2 ; month:08 ; pages:191-195 |
| Links: |
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| DOI / URN: |
10.1007/s002530051508 |
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| Katalog-ID: |
OLC2050685904 |
|---|
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| 100 | 1 | |a Khashnobish, A. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina |
| 264 | 1 | |c 1999 | |
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| 520 | |a Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. | ||
| 650 | 4 | |a Gene Expression | |
| 650 | 4 | |a Reporter Gene | |
| 650 | 4 | |a cDNA Library | |
| 650 | 4 | |a Repeat Unit | |
| 650 | 4 | |a Reporter Gene Expression | |
| 700 | 1 | |a Hamann, A. |4 aut | |
| 700 | 1 | |a Osiewacz, H. D. |4 aut | |
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10.1007/s002530051508 doi (DE-627)OLC2050685904 (DE-He213)s002530051508-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Khashnobish, A. verfasserin aut Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina 1999 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1999 Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. Gene Expression Reporter Gene cDNA Library Repeat Unit Reporter Gene Expression Hamann, A. aut Osiewacz, H. D. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 52(1999), 2 vom: Aug., Seite 191-195 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:52 year:1999 number:2 month:08 pages:191-195 https://doi.org/10.1007/s002530051508 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_147 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 52 1999 2 08 191-195 |
| spelling |
10.1007/s002530051508 doi (DE-627)OLC2050685904 (DE-He213)s002530051508-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Khashnobish, A. verfasserin aut Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina 1999 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1999 Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. Gene Expression Reporter Gene cDNA Library Repeat Unit Reporter Gene Expression Hamann, A. aut Osiewacz, H. D. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 52(1999), 2 vom: Aug., Seite 191-195 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:52 year:1999 number:2 month:08 pages:191-195 https://doi.org/10.1007/s002530051508 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_147 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 52 1999 2 08 191-195 |
| allfields_unstemmed |
10.1007/s002530051508 doi (DE-627)OLC2050685904 (DE-He213)s002530051508-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Khashnobish, A. verfasserin aut Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina 1999 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1999 Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. Gene Expression Reporter Gene cDNA Library Repeat Unit Reporter Gene Expression Hamann, A. aut Osiewacz, H. D. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 52(1999), 2 vom: Aug., Seite 191-195 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:52 year:1999 number:2 month:08 pages:191-195 https://doi.org/10.1007/s002530051508 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_147 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 52 1999 2 08 191-195 |
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10.1007/s002530051508 doi (DE-627)OLC2050685904 (DE-He213)s002530051508-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Khashnobish, A. verfasserin aut Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina 1999 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1999 Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. Gene Expression Reporter Gene cDNA Library Repeat Unit Reporter Gene Expression Hamann, A. aut Osiewacz, H. D. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 52(1999), 2 vom: Aug., Seite 191-195 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:52 year:1999 number:2 month:08 pages:191-195 https://doi.org/10.1007/s002530051508 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_147 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 52 1999 2 08 191-195 |
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10.1007/s002530051508 doi (DE-627)OLC2050685904 (DE-He213)s002530051508-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Khashnobish, A. verfasserin aut Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina 1999 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 1999 Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. Gene Expression Reporter Gene cDNA Library Repeat Unit Reporter Gene Expression Hamann, A. aut Osiewacz, H. D. aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 52(1999), 2 vom: Aug., Seite 191-195 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:52 year:1999 number:2 month:08 pages:191-195 https://doi.org/10.1007/s002530051508 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_130 GBV_ILN_147 GBV_ILN_252 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4028 GBV_ILN_4046 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4302 GBV_ILN_4307 GBV_ILN_4310 AR 52 1999 2 08 191-195 |
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Enthalten in Applied microbiology and biotechnology 52(1999), 2 vom: Aug., Seite 191-195 volume:52 year:1999 number:2 month:08 pages:191-195 |
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Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina |
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modulation of gene expression by (ca)n microsatellites in the filamentous ascomycete podospora anserina |
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Modulation of gene expression by (CA)n microsatellites in the filamentous ascomycete Podospora anserina |
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Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. © Springer-Verlag Berlin Heidelberg 1999 |
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Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. © Springer-Verlag Berlin Heidelberg 1999 |
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Abstract A microsatellite consisting of the alternating pyrimidine-purine sequence (CA)n.(TG)n is found to occur in very conserved form in the genome of various races of the filamentous ascomycete Podospora anserina. Screening of a cDNA library revealed that this sequence is frequently transcribed. In this study, we focused our attention on a short (CA)5 microsatellite located in the 5′ untranslated sequence of the glyceraldehyde-3-phosphate dehydrogenase (gpd) gene of P. anserina. Specifically, we investigated whether or not the number of repeat units present in the microsatellite affects the expression of the β-d-glucuronidase (gusA) reporter gene introduced on an autonomously replicating plasmid into fungal protoplasts. The results show that an increase in the number of microsatellite repeat units positively affects reporter gene expression. © Springer-Verlag Berlin Heidelberg 1999 |
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