Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization
Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rP...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wang, Yizhen [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2005 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2005 |
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| Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Springer Berlin Heidelberg, 1984, 69(2005), 4 vom: 27. Apr., Seite 385-389 |
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| Übergeordnetes Werk: |
volume:69 ; year:2005 ; number:4 ; day:27 ; month:04 ; pages:385-389 |
| Links: |
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| DOI / URN: |
10.1007/s00253-005-1998-y |
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| Katalog-ID: |
OLC2050704682 |
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| 520 | |a Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. | ||
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| 700 | 1 | |a Liu, Jianxin |4 aut | |
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10.1007/s00253-005-1998-y doi (DE-627)OLC2050704682 (DE-He213)s00253-005-1998-y-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Yizhen verfasserin aut Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2005 Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. Recombinant Baculovirus Baculovirus Expression System Bovine Lactoferrin Alkaline Phosphatase Conjugate Goat Baculovirus Transfer Vector Wu, Xiaofeng aut Liu, Guangfu aut Cao, Cuiping aut Huang, Haiqing aut Xu, Zirong aut Liu, Jianxin aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 69(2005), 4 vom: 27. Apr., Seite 385-389 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:69 year:2005 number:4 day:27 month:04 pages:385-389 https://doi.org/10.1007/s00253-005-1998-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 69 2005 4 27 04 385-389 |
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10.1007/s00253-005-1998-y doi (DE-627)OLC2050704682 (DE-He213)s00253-005-1998-y-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Yizhen verfasserin aut Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2005 Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. Recombinant Baculovirus Baculovirus Expression System Bovine Lactoferrin Alkaline Phosphatase Conjugate Goat Baculovirus Transfer Vector Wu, Xiaofeng aut Liu, Guangfu aut Cao, Cuiping aut Huang, Haiqing aut Xu, Zirong aut Liu, Jianxin aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 69(2005), 4 vom: 27. Apr., Seite 385-389 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:69 year:2005 number:4 day:27 month:04 pages:385-389 https://doi.org/10.1007/s00253-005-1998-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 69 2005 4 27 04 385-389 |
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10.1007/s00253-005-1998-y doi (DE-627)OLC2050704682 (DE-He213)s00253-005-1998-y-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Yizhen verfasserin aut Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2005 Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. Recombinant Baculovirus Baculovirus Expression System Bovine Lactoferrin Alkaline Phosphatase Conjugate Goat Baculovirus Transfer Vector Wu, Xiaofeng aut Liu, Guangfu aut Cao, Cuiping aut Huang, Haiqing aut Xu, Zirong aut Liu, Jianxin aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 69(2005), 4 vom: 27. Apr., Seite 385-389 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:69 year:2005 number:4 day:27 month:04 pages:385-389 https://doi.org/10.1007/s00253-005-1998-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 69 2005 4 27 04 385-389 |
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10.1007/s00253-005-1998-y doi (DE-627)OLC2050704682 (DE-He213)s00253-005-1998-y-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Yizhen verfasserin aut Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2005 Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. Recombinant Baculovirus Baculovirus Expression System Bovine Lactoferrin Alkaline Phosphatase Conjugate Goat Baculovirus Transfer Vector Wu, Xiaofeng aut Liu, Guangfu aut Cao, Cuiping aut Huang, Haiqing aut Xu, Zirong aut Liu, Jianxin aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 69(2005), 4 vom: 27. Apr., Seite 385-389 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:69 year:2005 number:4 day:27 month:04 pages:385-389 https://doi.org/10.1007/s00253-005-1998-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 69 2005 4 27 04 385-389 |
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10.1007/s00253-005-1998-y doi (DE-627)OLC2050704682 (DE-He213)s00253-005-1998-y-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Yizhen verfasserin aut Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization 2005 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2005 Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. Recombinant Baculovirus Baculovirus Expression System Bovine Lactoferrin Alkaline Phosphatase Conjugate Goat Baculovirus Transfer Vector Wu, Xiaofeng aut Liu, Guangfu aut Cao, Cuiping aut Huang, Haiqing aut Xu, Zirong aut Liu, Jianxin aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 69(2005), 4 vom: 27. Apr., Seite 385-389 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:69 year:2005 number:4 day:27 month:04 pages:385-389 https://doi.org/10.1007/s00253-005-1998-y lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 69 2005 4 27 04 385-389 |
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Enthalten in Applied microbiology and biotechnology 69(2005), 4 vom: 27. Apr., Seite 385-389 volume:69 year:2005 number:4 day:27 month:04 pages:385-389 |
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Enthalten in Applied microbiology and biotechnology 69(2005), 4 vom: 27. Apr., Seite 385-389 volume:69 year:2005 number:4 day:27 month:04 pages:385-389 |
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Recombinant Baculovirus Baculovirus Expression System Bovine Lactoferrin Alkaline Phosphatase Conjugate Goat Baculovirus Transfer Vector |
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Wang, Yizhen @@aut@@ Wu, Xiaofeng @@aut@@ Liu, Guangfu @@aut@@ Cao, Cuiping @@aut@@ Huang, Haiqing @@aut@@ Xu, Zirong @@aut@@ Liu, Jianxin @@aut@@ |
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expression of porcine lactoferrin by using recombinant baculovirus in silkworm, bombyx mori l., and its purification and characterization |
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Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization |
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Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. © Springer-Verlag 2005 |
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Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. © Springer-Verlag 2005 |
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Abstract Lactoferrin is a multifunctional glycoprotein that is present in several mucosal secretions. In this study, we exploited the silkworm, Bombyx mori, as host for the recombinant baculovirus harboring the porcine lactoferrin (PLF) gene to produce the recombinant PLF (rPLF). Around 205 μg of rPLF was purified from a single silkworm pupa infected by the virus and the rPLF was proved to be biologically active. This method established in our study will pave the way for efficient industrial production of rPLF on a large scale for further utilization of this protein as a feed additive in the future. © Springer-Verlag 2005 |
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