Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana
Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integ...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ying, Sheng-Hua [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2006 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag 2006 |
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| Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Springer Berlin Heidelberg, 1984, 72(2006), 1 vom: 26. Apr., Seite 206-210 |
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| Übergeordnetes Werk: |
volume:72 ; year:2006 ; number:1 ; day:26 ; month:04 ; pages:206-210 |
| Links: |
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| DOI / URN: |
10.1007/s00253-006-0447-x |
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| Katalog-ID: |
OLC2050707223 |
|---|
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| 245 | 1 | 0 | |a Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana |
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| 520 | |a Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. | ||
| 650 | 4 | |a Phosphinothricin | |
| 650 | 4 | |a Putative Transformants | |
| 650 | 4 | |a Egfp Gene | |
| 650 | 4 | |a Beauveria Bassiana | |
| 650 | 4 | |a Nitrate Reductase Gene | |
| 700 | 1 | |a Feng, Ming-Guang |4 aut | |
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10.1007/s00253-006-0447-x doi (DE-627)OLC2050707223 (DE-He213)s00253-006-0447-x-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ying, Sheng-Hua verfasserin aut Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2006 Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. Phosphinothricin Putative Transformants Egfp Gene Beauveria Bassiana Nitrate Reductase Gene Feng, Ming-Guang aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 72(2006), 1 vom: 26. Apr., Seite 206-210 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:72 year:2006 number:1 day:26 month:04 pages:206-210 https://doi.org/10.1007/s00253-006-0447-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 72 2006 1 26 04 206-210 |
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10.1007/s00253-006-0447-x doi (DE-627)OLC2050707223 (DE-He213)s00253-006-0447-x-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ying, Sheng-Hua verfasserin aut Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2006 Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. Phosphinothricin Putative Transformants Egfp Gene Beauveria Bassiana Nitrate Reductase Gene Feng, Ming-Guang aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 72(2006), 1 vom: 26. Apr., Seite 206-210 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:72 year:2006 number:1 day:26 month:04 pages:206-210 https://doi.org/10.1007/s00253-006-0447-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 72 2006 1 26 04 206-210 |
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10.1007/s00253-006-0447-x doi (DE-627)OLC2050707223 (DE-He213)s00253-006-0447-x-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ying, Sheng-Hua verfasserin aut Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2006 Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. Phosphinothricin Putative Transformants Egfp Gene Beauveria Bassiana Nitrate Reductase Gene Feng, Ming-Guang aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 72(2006), 1 vom: 26. Apr., Seite 206-210 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:72 year:2006 number:1 day:26 month:04 pages:206-210 https://doi.org/10.1007/s00253-006-0447-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 72 2006 1 26 04 206-210 |
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10.1007/s00253-006-0447-x doi (DE-627)OLC2050707223 (DE-He213)s00253-006-0447-x-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ying, Sheng-Hua verfasserin aut Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2006 Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. Phosphinothricin Putative Transformants Egfp Gene Beauveria Bassiana Nitrate Reductase Gene Feng, Ming-Guang aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 72(2006), 1 vom: 26. Apr., Seite 206-210 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:72 year:2006 number:1 day:26 month:04 pages:206-210 https://doi.org/10.1007/s00253-006-0447-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 72 2006 1 26 04 206-210 |
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10.1007/s00253-006-0447-x doi (DE-627)OLC2050707223 (DE-He213)s00253-006-0447-x-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ying, Sheng-Hua verfasserin aut Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana 2006 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2006 Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. Phosphinothricin Putative Transformants Egfp Gene Beauveria Bassiana Nitrate Reductase Gene Feng, Ming-Guang aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 72(2006), 1 vom: 26. Apr., Seite 206-210 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:72 year:2006 number:1 day:26 month:04 pages:206-210 https://doi.org/10.1007/s00253-006-0447-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_31 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4155 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 72 2006 1 26 04 206-210 |
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English |
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Enthalten in Applied microbiology and biotechnology 72(2006), 1 vom: 26. Apr., Seite 206-210 volume:72 year:2006 number:1 day:26 month:04 pages:206-210 |
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Enthalten in Applied microbiology and biotechnology 72(2006), 1 vom: 26. Apr., Seite 206-210 volume:72 year:2006 number:1 day:26 month:04 pages:206-210 |
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Phosphinothricin Putative Transformants Egfp Gene Beauveria Bassiana Nitrate Reductase Gene |
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Ying, Sheng-Hua @@aut@@ Feng, Ming-Guang @@aut@@ |
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570 VZ 12 ssgn BIODIV DE-30 fid Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana Phosphinothricin Putative Transformants Egfp Gene Beauveria Bassiana Nitrate Reductase Gene |
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novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into beauveria bassiana |
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Novel blastospore-based transformation system for integration of phosphinothricin resistance and green fluorescence protein genes into Beauveria bassiana |
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Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. © Springer-Verlag 2006 |
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Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. © Springer-Verlag 2006 |
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Abstract A novel system was developed for efficient transformation of the fungal biocontrol agent Beauveriabassiana. Competent blastospores were prepared and stored in LiAc- and glycerol-inclusive suspension at −76 °C for sequential use in transformation. The system was successfully applied to integrating phosphinothricin resistance gene bar and enhanced green fluorescence protein gene egfp into B. bassiana via blastospore absorption of a plasmid vectoring bar and egfp. A frequency of 24 transformants per microgram of DNA was achieved. The blastospore-based transformation system has proven to be very convenient and would be highly potential for use in genetic manipulation of B. bassiana and other filamentous species. © Springer-Verlag 2006 |
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