Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures
Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growt...
Ausführliche Beschreibung
Autor*in: |
Wang, Jin [verfasserIn] |
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Format: |
Artikel |
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Sprache: |
Englisch |
Erschienen: |
2007 |
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Schlagwörter: |
Extracellular Polymeric Substance |
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Anmerkung: |
© Springer-Verlag 2007 |
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Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Springer Berlin Heidelberg, 1984, 75(2007), 4 vom: 21. Feb., Seite 871-878 |
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Übergeordnetes Werk: |
volume:75 ; year:2007 ; number:4 ; day:21 ; month:02 ; pages:871-878 |
Links: |
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DOI / URN: |
10.1007/s00253-007-0870-7 |
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Katalog-ID: |
OLC2050713150 |
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10.1007/s00253-007-0870-7 doi (DE-627)OLC2050713150 (DE-He213)s00253-007-0870-7-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Jin verfasserin aut Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2007 Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and ($ NH_{4} $)2$ SO_{4} $ of 3.2 g/l. Extracellular Polymeric Substance Biomass Growth 2SO4 Concentration Extracellular Polymeric Substance Production Flocculation Ability Yu, Han-Qing aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 75(2007), 4 vom: 21. Feb., Seite 871-878 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:75 year:2007 number:4 day:21 month:02 pages:871-878 https://doi.org/10.1007/s00253-007-0870-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 75 2007 4 21 02 871-878 |
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10.1007/s00253-007-0870-7 doi (DE-627)OLC2050713150 (DE-He213)s00253-007-0870-7-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Jin verfasserin aut Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2007 Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and ($ NH_{4} $)2$ SO_{4} $ of 3.2 g/l. Extracellular Polymeric Substance Biomass Growth 2SO4 Concentration Extracellular Polymeric Substance Production Flocculation Ability Yu, Han-Qing aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 75(2007), 4 vom: 21. Feb., Seite 871-878 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:75 year:2007 number:4 day:21 month:02 pages:871-878 https://doi.org/10.1007/s00253-007-0870-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 75 2007 4 21 02 871-878 |
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10.1007/s00253-007-0870-7 doi (DE-627)OLC2050713150 (DE-He213)s00253-007-0870-7-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Jin verfasserin aut Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2007 Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and ($ NH_{4} $)2$ SO_{4} $ of 3.2 g/l. Extracellular Polymeric Substance Biomass Growth 2SO4 Concentration Extracellular Polymeric Substance Production Flocculation Ability Yu, Han-Qing aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 75(2007), 4 vom: 21. Feb., Seite 871-878 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:75 year:2007 number:4 day:21 month:02 pages:871-878 https://doi.org/10.1007/s00253-007-0870-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 75 2007 4 21 02 871-878 |
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10.1007/s00253-007-0870-7 doi (DE-627)OLC2050713150 (DE-He213)s00253-007-0870-7-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Jin verfasserin aut Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2007 Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and ($ NH_{4} $)2$ SO_{4} $ of 3.2 g/l. Extracellular Polymeric Substance Biomass Growth 2SO4 Concentration Extracellular Polymeric Substance Production Flocculation Ability Yu, Han-Qing aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 75(2007), 4 vom: 21. Feb., Seite 871-878 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:75 year:2007 number:4 day:21 month:02 pages:871-878 https://doi.org/10.1007/s00253-007-0870-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 75 2007 4 21 02 871-878 |
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10.1007/s00253-007-0870-7 doi (DE-627)OLC2050713150 (DE-He213)s00253-007-0870-7-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Wang, Jin verfasserin aut Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures 2007 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2007 Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and ($ NH_{4} $)2$ SO_{4} $ of 3.2 g/l. Extracellular Polymeric Substance Biomass Growth 2SO4 Concentration Extracellular Polymeric Substance Production Flocculation Ability Yu, Han-Qing aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 75(2007), 4 vom: 21. Feb., Seite 871-878 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:75 year:2007 number:4 day:21 month:02 pages:871-878 https://doi.org/10.1007/s00253-007-0870-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 75 2007 4 21 02 871-878 |
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Enthalten in Applied microbiology and biotechnology 75(2007), 4 vom: 21. Feb., Seite 871-878 volume:75 year:2007 number:4 day:21 month:02 pages:871-878 |
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Enthalten in Applied microbiology and biotechnology 75(2007), 4 vom: 21. Feb., Seite 871-878 volume:75 year:2007 number:4 day:21 month:02 pages:871-878 |
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Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures |
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biosynthesis of polyhydroxybutyrate (phb) and extracellular polymeric substances (eps) by ralstonia eutropha atcc 17699 in batch cultures |
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Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures |
abstract |
Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and ($ NH_{4} $)2$ SO_{4} $ of 3.2 g/l. © Springer-Verlag 2007 |
abstractGer |
Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and ($ NH_{4} $)2$ SO_{4} $ of 3.2 g/l. © Springer-Verlag 2007 |
abstract_unstemmed |
Abstract The production of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 at various glucose and ($ NH_{4} $)2$ SO_{4} $ concentrations in batch cultures were investigated. The biosynthesis of EPS by R. eutropha closely coupled with cell growth, while PHB was synthesized only under nitrogen-deficient and cell-growth-limited conditions. Experimental results show that the specific PHB production rate had an exponential correlation with both specific cell growth rate and EPS production rate. Furthermore, PHB was observed as the main storage of carbon and energy source by R. eutropha under nitrogen-limited conditions. In addition, experiments were conducted based on central composite design to optimize the batch culture for a high PHB yield. The PHB yield on glucose reached a maximum value of 0.34 g/g at glucose concentrations of 38.2 g/l and ($ NH_{4} $)2$ SO_{4} $ of 3.2 g/l. © Springer-Verlag 2007 |
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Biosynthesis of polyhydroxybutyrate (PHB) and extracellular polymeric substances (EPS) by Ralstonia eutropha ATCC 17699 in batch cultures |
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