Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside

Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous...
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Gespeichert in:

Autor*in:	Yu, Cigang [verfasserIn] Xu, Haidong Huang, Guodong Chen, Ting Liu, Guiyou Chai, Nan Ji, Yin Wang, Siyuan Dai, Yijun Yuan, Sheng

Format:	Artikel
Sprache:	Englisch

Erschienen:	2009

Schlagwörter:	Puerarin Puerarin-7- -glucoside -fructoside Conversion Permeabilization

Anmerkung:	© Springer-Verlag 2009

Übergeordnetes Werk:	Enthalten in: Applied microbiology and biotechnology - Springer-Verlag, 1984, 86(2009), 3 vom: 27. Nov., Seite 863-870
Übergeordnetes Werk:	volume:86 ; year:2009 ; number:3 ; day:27 ; month:11 ; pages:863-870

Links:	Volltext

DOI / URN:	10.1007/s00253-009-2341-9

Katalog-ID:	OLC2050727542

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245	1	0	\|a Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside
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520			\|a Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition.
650		4	\|a Puerarin
650		4	\|a Puerarin-7-
650		4	\|a -glucoside
650		4	\|a Puerarin-7-
650		4	\|a -fructoside
650		4	\|a Conversion
650		4	\|a Permeabilization
700	1		\|a Xu, Haidong \|4 aut
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700	1		\|a Chen, Ting \|4 aut
700	1		\|a Liu, Guiyou \|4 aut
700	1		\|a Chai, Nan \|4 aut
700	1		\|a Ji, Yin \|4 aut
700	1		\|a Wang, Siyuan \|4 aut
700	1		\|a Dai, Yijun \|4 aut
700	1		\|a Yuan, Sheng \|4 aut
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Indexfelder

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allfields	10.1007/s00253-009-2341-9 doi (DE-627)OLC2050727542 (DE-He213)s00253-009-2341-9-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Yu, Cigang verfasserin aut Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition. Puerarin Puerarin-7- -glucoside Puerarin-7- -fructoside Conversion Permeabilization Xu, Haidong aut Huang, Guodong aut Chen, Ting aut Liu, Guiyou aut Chai, Nan aut Ji, Yin aut Wang, Siyuan aut Dai, Yijun aut Yuan, Sheng aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 86(2009), 3 vom: 27. Nov., Seite 863-870 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:86 year:2009 number:3 day:27 month:11 pages:863-870 https://doi.org/10.1007/s00253-009-2341-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 86 2009 3 27 11 863-870
spelling	10.1007/s00253-009-2341-9 doi (DE-627)OLC2050727542 (DE-He213)s00253-009-2341-9-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Yu, Cigang verfasserin aut Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition. Puerarin Puerarin-7- -glucoside Puerarin-7- -fructoside Conversion Permeabilization Xu, Haidong aut Huang, Guodong aut Chen, Ting aut Liu, Guiyou aut Chai, Nan aut Ji, Yin aut Wang, Siyuan aut Dai, Yijun aut Yuan, Sheng aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 86(2009), 3 vom: 27. Nov., Seite 863-870 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:86 year:2009 number:3 day:27 month:11 pages:863-870 https://doi.org/10.1007/s00253-009-2341-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 86 2009 3 27 11 863-870
allfields_unstemmed	10.1007/s00253-009-2341-9 doi (DE-627)OLC2050727542 (DE-He213)s00253-009-2341-9-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Yu, Cigang verfasserin aut Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition. Puerarin Puerarin-7- -glucoside Puerarin-7- -fructoside Conversion Permeabilization Xu, Haidong aut Huang, Guodong aut Chen, Ting aut Liu, Guiyou aut Chai, Nan aut Ji, Yin aut Wang, Siyuan aut Dai, Yijun aut Yuan, Sheng aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 86(2009), 3 vom: 27. Nov., Seite 863-870 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:86 year:2009 number:3 day:27 month:11 pages:863-870 https://doi.org/10.1007/s00253-009-2341-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 86 2009 3 27 11 863-870
allfieldsGer	10.1007/s00253-009-2341-9 doi (DE-627)OLC2050727542 (DE-He213)s00253-009-2341-9-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Yu, Cigang verfasserin aut Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition. Puerarin Puerarin-7- -glucoside Puerarin-7- -fructoside Conversion Permeabilization Xu, Haidong aut Huang, Guodong aut Chen, Ting aut Liu, Guiyou aut Chai, Nan aut Ji, Yin aut Wang, Siyuan aut Dai, Yijun aut Yuan, Sheng aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 86(2009), 3 vom: 27. Nov., Seite 863-870 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:86 year:2009 number:3 day:27 month:11 pages:863-870 https://doi.org/10.1007/s00253-009-2341-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 86 2009 3 27 11 863-870
allfieldsSound	10.1007/s00253-009-2341-9 doi (DE-627)OLC2050727542 (DE-He213)s00253-009-2341-9-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Yu, Cigang verfasserin aut Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag 2009 Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition. Puerarin Puerarin-7- -glucoside Puerarin-7- -fructoside Conversion Permeabilization Xu, Haidong aut Huang, Guodong aut Chen, Ting aut Liu, Guiyou aut Chai, Nan aut Ji, Yin aut Wang, Siyuan aut Dai, Yijun aut Yuan, Sheng aut Enthalten in Applied microbiology and biotechnology Springer-Verlag, 1984 86(2009), 3 vom: 27. Nov., Seite 863-870 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:86 year:2009 number:3 day:27 month:11 pages:863-870 https://doi.org/10.1007/s00253-009-2341-9 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307 AR 86 2009 3 27 11 863-870
language	English
source	Enthalten in Applied microbiology and biotechnology 86(2009), 3 vom: 27. Nov., Seite 863-870 volume:86 year:2009 number:3 day:27 month:11 pages:863-870
sourceStr	Enthalten in Applied microbiology and biotechnology 86(2009), 3 vom: 27. Nov., Seite 863-870 volume:86 year:2009 number:3 day:27 month:11 pages:863-870
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Puerarin Puerarin-7- -glucoside -fructoside Conversion Permeabilization
dewey-raw	570
isfreeaccess_bool	false
container_title	Applied microbiology and biotechnology
authorswithroles_txt_mv	Yu, Cigang @@aut@@ Xu, Haidong @@aut@@ Huang, Guodong @@aut@@ Chen, Ting @@aut@@ Liu, Guiyou @@aut@@ Chai, Nan @@aut@@ Ji, Yin @@aut@@ Wang, Siyuan @@aut@@ Dai, Yijun @@aut@@ Yuan, Sheng @@aut@@
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author	Yu, Cigang
spellingShingle	Yu, Cigang ddc 570 ssgn 12 fid BIODIV misc Puerarin misc Puerarin-7- misc -glucoside misc -fructoside misc Conversion misc Permeabilization Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside
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topic_title	570 VZ 12 ssgn BIODIV DE-30 fid Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside Puerarin Puerarin-7- -glucoside -fructoside Conversion Permeabilization
topic	ddc 570 ssgn 12 fid BIODIV misc Puerarin misc Puerarin-7- misc -glucoside misc -fructoside misc Conversion misc Permeabilization
topic_unstemmed	ddc 570 ssgn 12 fid BIODIV misc Puerarin misc Puerarin-7- misc -glucoside misc -fructoside misc Conversion misc Permeabilization
topic_browse	ddc 570 ssgn 12 fid BIODIV misc Puerarin misc Puerarin-7- misc -glucoside misc -fructoside misc Conversion misc Permeabilization
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title	Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside
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title_full	Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside
author_sort	Yu, Cigang
journal	Applied microbiology and biotechnology
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author_browse	Yu, Cigang Xu, Haidong Huang, Guodong Chen, Ting Liu, Guiyou Chai, Nan Ji, Yin Wang, Siyuan Dai, Yijun Yuan, Sheng
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title_sort	permeabilization of microbacterium oxylans shifts the conversion of puerarin from puerarin-7-o-glucoside to puerarin-7-o-fructoside
title_auth	Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside
abstract	Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition. © Springer-Verlag 2009
abstractGer	Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition. © Springer-Verlag 2009
abstract_unstemmed	Abstract The main product of the conversion of puerarin by unpermeabilized cells of bacterium Microbacterium oxydans CGMCC 1788 was puerarin-7-O-glucoside (241 ± 31.9 µM). Permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 ± 48.0 µM) which was identified as a novel puerarin-7-O-fructoside by electrospray ionization time-of-flight MS, 13C NMR, 1H NMR, and GC-MS analysis of sugar composition, and puerarin-7-O-glucoside became a trace product (14.8 ± 5.4 µM). However, the extract from cells of M. oxydans CGMCC 1788 permeabilized with ethanol converted puerarin to form 113.9 ± 27.7 µM puerarin-7-O-glucoside and 187.8 ± 29.5 µM puerarin-7-O-fructoside under the same conditions. When unpermeabilized intact cells were recovered and used repeatedly for the conversion of puerarin, with increase of reuse times, the yield of puerarin-7-O-glucoside gradually decreased, whereas the yield of puerarin-7-O-fructoside increased gradually in the conversion mixture. The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). Therefore, the change of permeability of cell membrane of bacterium M. oxydans CGMCC 1788 contributed to the change of conversion of the product’s composition. © Springer-Verlag 2009
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_40 GBV_ILN_69 GBV_ILN_70 GBV_ILN_100 GBV_ILN_130 GBV_ILN_147 GBV_ILN_267 GBV_ILN_285 GBV_ILN_2004 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4307
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title_short	Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside
url	https://doi.org/10.1007/s00253-009-2341-9
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author2	Xu, Haidong Huang, Guodong Chen, Ting Liu, Guiyou Chai, Nan Ji, Yin Wang, Siyuan Dai, Yijun Yuan, Sheng
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up_date	2024-07-04T02:42:06.328Z
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The main product of the conversion of puerarin by the tenth recycled unpremerbilized cells was puerarin-7-O-fructoside (288.4 ± 24.0 µM). 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Permeabilization of Microbacterium oxylans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside

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