Improved α-amylase production by Aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression
Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this...
Ausführliche Beschreibung
Autor*in: |
Ichinose, Sakurako [verfasserIn] |
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Format: |
Artikel |
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Sprache: |
Englisch |
Erschienen: |
2013 |
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Schlagwörter: |
Carbon catabolite derepression |
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Anmerkung: |
© Springer-Verlag Berlin Heidelberg 2013 |
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Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Springer Berlin Heidelberg, 1984, 98(2013), 1 vom: 10. Nov., Seite 335-343 |
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Übergeordnetes Werk: |
volume:98 ; year:2013 ; number:1 ; day:10 ; month:11 ; pages:335-343 |
Links: |
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DOI / URN: |
10.1007/s00253-013-5353-4 |
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Katalog-ID: |
OLC2050755260 |
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520 | |a Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. | ||
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650 | 4 | |a CreA transcription repressor | |
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650 | 4 | |a Double deletion mutant | |
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700 | 1 | |a Gomi, Katsuya |4 aut | |
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10.1007/s00253-013-5353-4 doi (DE-627)OLC2050755260 (DE-He213)s00253-013-5353-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ichinose, Sakurako verfasserin aut Improved α-amylase production by Aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. α-Amylase production Carbon catabolite derepression CreA transcription repressor CreB ubiquitin processing protease Double deletion mutant Tanaka, Mizuki aut Shintani, Takahiro aut Gomi, Katsuya aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 98(2013), 1 vom: 10. Nov., Seite 335-343 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:98 year:2013 number:1 day:10 month:11 pages:335-343 https://doi.org/10.1007/s00253-013-5353-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 AR 98 2013 1 10 11 335-343 |
spelling |
10.1007/s00253-013-5353-4 doi (DE-627)OLC2050755260 (DE-He213)s00253-013-5353-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ichinose, Sakurako verfasserin aut Improved α-amylase production by Aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. α-Amylase production Carbon catabolite derepression CreA transcription repressor CreB ubiquitin processing protease Double deletion mutant Tanaka, Mizuki aut Shintani, Takahiro aut Gomi, Katsuya aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 98(2013), 1 vom: 10. Nov., Seite 335-343 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:98 year:2013 number:1 day:10 month:11 pages:335-343 https://doi.org/10.1007/s00253-013-5353-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 AR 98 2013 1 10 11 335-343 |
allfields_unstemmed |
10.1007/s00253-013-5353-4 doi (DE-627)OLC2050755260 (DE-He213)s00253-013-5353-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ichinose, Sakurako verfasserin aut Improved α-amylase production by Aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. α-Amylase production Carbon catabolite derepression CreA transcription repressor CreB ubiquitin processing protease Double deletion mutant Tanaka, Mizuki aut Shintani, Takahiro aut Gomi, Katsuya aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 98(2013), 1 vom: 10. Nov., Seite 335-343 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:98 year:2013 number:1 day:10 month:11 pages:335-343 https://doi.org/10.1007/s00253-013-5353-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 AR 98 2013 1 10 11 335-343 |
allfieldsGer |
10.1007/s00253-013-5353-4 doi (DE-627)OLC2050755260 (DE-He213)s00253-013-5353-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ichinose, Sakurako verfasserin aut Improved α-amylase production by Aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. α-Amylase production Carbon catabolite derepression CreA transcription repressor CreB ubiquitin processing protease Double deletion mutant Tanaka, Mizuki aut Shintani, Takahiro aut Gomi, Katsuya aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 98(2013), 1 vom: 10. Nov., Seite 335-343 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:98 year:2013 number:1 day:10 month:11 pages:335-343 https://doi.org/10.1007/s00253-013-5353-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 AR 98 2013 1 10 11 335-343 |
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10.1007/s00253-013-5353-4 doi (DE-627)OLC2050755260 (DE-He213)s00253-013-5353-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Ichinose, Sakurako verfasserin aut Improved α-amylase production by Aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2013 Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. α-Amylase production Carbon catabolite derepression CreA transcription repressor CreB ubiquitin processing protease Double deletion mutant Tanaka, Mizuki aut Shintani, Takahiro aut Gomi, Katsuya aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 98(2013), 1 vom: 10. Nov., Seite 335-343 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:98 year:2013 number:1 day:10 month:11 pages:335-343 https://doi.org/10.1007/s00253-013-5353-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_23 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 AR 98 2013 1 10 11 335-343 |
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English |
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Enthalten in Applied microbiology and biotechnology 98(2013), 1 vom: 10. Nov., Seite 335-343 volume:98 year:2013 number:1 day:10 month:11 pages:335-343 |
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Enthalten in Applied microbiology and biotechnology 98(2013), 1 vom: 10. Nov., Seite 335-343 volume:98 year:2013 number:1 day:10 month:11 pages:335-343 |
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α-Amylase production Carbon catabolite derepression CreA transcription repressor CreB ubiquitin processing protease Double deletion mutant |
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Ichinose, Sakurako @@aut@@ Tanaka, Mizuki @@aut@@ Shintani, Takahiro @@aut@@ Gomi, Katsuya @@aut@@ |
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improved α-amylase production by aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression |
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Improved α-amylase production by Aspergillus oryzae after a double deletion of genes involved in carbon catabolite repression |
abstract |
Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. © Springer-Verlag Berlin Heidelberg 2013 |
abstractGer |
Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. © Springer-Verlag Berlin Heidelberg 2013 |
abstract_unstemmed |
Abstract In filamentous fungi, the expression of secretory glycoside hydrolase encoding genes, such as those for amylases, cellulases, and xylanases, is generally repressed in the presence of glucose. CreA and CreB have been observed to be regulating factors for carbon catabolite repression. In this study, we generated single and double deletion creA and/or creB mutants in Aspergillus oryzae. The α-amylase activities of each strain were compared under various culture conditions. For the wild-type strain, mRNA levels of α-amylase were markedly decreased in the later stage of submerged culture under inducing conditions, whereas this reduced expression was not observed for single creA and double creA/creB deletion mutants. In addition, α-amylase activity of the wild-type strain was reduced in submerged culture containing high concentrations of inducing sugars, whereas all constructed mutants showed higher α-amylase activities. In particular, the α-amylase activity of the double deletion mutant in a medium containing 5 % starch was >10-fold higher than that of the wild-type strain under the same culture conditions. In solid-state cultures using wheat bran as a substrate, the α-amylase activities of single creA and double deletion mutants were >2-fold higher than that of the wild-type strain. These results suggested that deleting both creA and creB resulted in dramatic improvements in the production of secretory glycoside hydrolases in filamentous fungi. © Springer-Verlag Berlin Heidelberg 2013 |
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