Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi
Abstract Filamentous fungi, particularly Aspergillus species, have recently attracted attention as host organisms for recombinant protein production. Because the secretory yields of heterologous proteins are generally low compared with those of homologous proteins or proteins from closely related fu...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Tanaka, Mizuki [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer-Verlag Berlin Heidelberg 2014 |
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| Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Springer Berlin Heidelberg, 1984, 98(2014), 9 vom: 28. Feb., Seite 3859-3867 |
|---|---|
| Übergeordnetes Werk: |
volume:98 ; year:2014 ; number:9 ; day:28 ; month:02 ; pages:3859-3867 |
| Links: |
|---|
| DOI / URN: |
10.1007/s00253-014-5609-7 |
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| Katalog-ID: |
OLC2050758669 |
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| 520 | |a Abstract Filamentous fungi, particularly Aspergillus species, have recently attracted attention as host organisms for recombinant protein production. Because the secretory yields of heterologous proteins are generally low compared with those of homologous proteins or proteins from closely related fungal species, several strategies to produce substantial amounts of recombinant proteins have been conducted. Codon optimization is a powerful tool for improving the production levels of heterologous proteins. Although codon optimization is generally believed to improve the translation efficiency of heterologous genes without affecting their mRNA levels, several studies have indicated that codon optimization causes an increase in the steady-state mRNA levels of heterologous genes in filamentous fungi. However, the mechanism that determines the low mRNA levels when native heterologous genes are expressed was poorly understood. We recently showed that the transcripts of heterologous genes are polyadenylated prematurely within the coding region and that the heterologous gene transcripts can be stabilized significantly by codon optimization, which is probably attributable to the prevention of premature polyadenylation in Aspergillus oryzae. In this review, we describe the detailed mechanism of premature polyadenylation and the rapid degradation of mRNA transcripts derived from heterologous genes in filamentous fungi. | ||
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| 650 | 4 | |a Codon optimization | |
| 650 | 4 | |a Steady-state mRNA level | |
| 650 | 4 | |a Polyadenylation site | |
| 650 | 4 | |a 3′-end processing signal | |
| 650 | 4 | |a Filamentous fungi | |
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| 700 | 1 | |a Gomi, Katsuya |4 aut | |
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10.1007/s00253-014-5609-7 doi (DE-627)OLC2050758669 (DE-He213)s00253-014-5609-7-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Tanaka, Mizuki verfasserin aut Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2014 Abstract Filamentous fungi, particularly Aspergillus species, have recently attracted attention as host organisms for recombinant protein production. Because the secretory yields of heterologous proteins are generally low compared with those of homologous proteins or proteins from closely related fungal species, several strategies to produce substantial amounts of recombinant proteins have been conducted. Codon optimization is a powerful tool for improving the production levels of heterologous proteins. Although codon optimization is generally believed to improve the translation efficiency of heterologous genes without affecting their mRNA levels, several studies have indicated that codon optimization causes an increase in the steady-state mRNA levels of heterologous genes in filamentous fungi. However, the mechanism that determines the low mRNA levels when native heterologous genes are expressed was poorly understood. We recently showed that the transcripts of heterologous genes are polyadenylated prematurely within the coding region and that the heterologous gene transcripts can be stabilized significantly by codon optimization, which is probably attributable to the prevention of premature polyadenylation in Aspergillus oryzae. In this review, we describe the detailed mechanism of premature polyadenylation and the rapid degradation of mRNA transcripts derived from heterologous genes in filamentous fungi. Heterologous protein production Codon optimization Steady-state mRNA level Polyadenylation site 3′-end processing signal Filamentous fungi Tokuoka, Masafumi aut Gomi, Katsuya aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 98(2014), 9 vom: 28. Feb., Seite 3859-3867 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:98 year:2014 number:9 day:28 month:02 pages:3859-3867 https://doi.org/10.1007/s00253-014-5609-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 AR 98 2014 9 28 02 3859-3867 |
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10.1007/s00253-014-5609-7 doi (DE-627)OLC2050758669 (DE-He213)s00253-014-5609-7-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Tanaka, Mizuki verfasserin aut Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi 2014 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer-Verlag Berlin Heidelberg 2014 Abstract Filamentous fungi, particularly Aspergillus species, have recently attracted attention as host organisms for recombinant protein production. Because the secretory yields of heterologous proteins are generally low compared with those of homologous proteins or proteins from closely related fungal species, several strategies to produce substantial amounts of recombinant proteins have been conducted. Codon optimization is a powerful tool for improving the production levels of heterologous proteins. Although codon optimization is generally believed to improve the translation efficiency of heterologous genes without affecting their mRNA levels, several studies have indicated that codon optimization causes an increase in the steady-state mRNA levels of heterologous genes in filamentous fungi. However, the mechanism that determines the low mRNA levels when native heterologous genes are expressed was poorly understood. We recently showed that the transcripts of heterologous genes are polyadenylated prematurely within the coding region and that the heterologous gene transcripts can be stabilized significantly by codon optimization, which is probably attributable to the prevention of premature polyadenylation in Aspergillus oryzae. In this review, we describe the detailed mechanism of premature polyadenylation and the rapid degradation of mRNA transcripts derived from heterologous genes in filamentous fungi. Heterologous protein production Codon optimization Steady-state mRNA level Polyadenylation site 3′-end processing signal Filamentous fungi Tokuoka, Masafumi aut Gomi, Katsuya aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 98(2014), 9 vom: 28. Feb., Seite 3859-3867 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:98 year:2014 number:9 day:28 month:02 pages:3859-3867 https://doi.org/10.1007/s00253-014-5609-7 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4082 GBV_ILN_4277 GBV_ILN_4305 AR 98 2014 9 28 02 3859-3867 |
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570 VZ 12 ssgn BIODIV DE-30 fid Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi Heterologous protein production Codon optimization Steady-state mRNA level Polyadenylation site 3′-end processing signal Filamentous fungi |
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Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi |
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Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi |
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Tanaka, Mizuki |
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Applied microbiology and biotechnology |
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Tanaka, Mizuki Tokuoka, Masafumi Gomi, Katsuya |
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effects of codon optimization on the mrna levels of heterologous genes in filamentous fungi |
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Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi |
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Abstract Filamentous fungi, particularly Aspergillus species, have recently attracted attention as host organisms for recombinant protein production. Because the secretory yields of heterologous proteins are generally low compared with those of homologous proteins or proteins from closely related fungal species, several strategies to produce substantial amounts of recombinant proteins have been conducted. Codon optimization is a powerful tool for improving the production levels of heterologous proteins. Although codon optimization is generally believed to improve the translation efficiency of heterologous genes without affecting their mRNA levels, several studies have indicated that codon optimization causes an increase in the steady-state mRNA levels of heterologous genes in filamentous fungi. However, the mechanism that determines the low mRNA levels when native heterologous genes are expressed was poorly understood. We recently showed that the transcripts of heterologous genes are polyadenylated prematurely within the coding region and that the heterologous gene transcripts can be stabilized significantly by codon optimization, which is probably attributable to the prevention of premature polyadenylation in Aspergillus oryzae. In this review, we describe the detailed mechanism of premature polyadenylation and the rapid degradation of mRNA transcripts derived from heterologous genes in filamentous fungi. © Springer-Verlag Berlin Heidelberg 2014 |
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Abstract Filamentous fungi, particularly Aspergillus species, have recently attracted attention as host organisms for recombinant protein production. Because the secretory yields of heterologous proteins are generally low compared with those of homologous proteins or proteins from closely related fungal species, several strategies to produce substantial amounts of recombinant proteins have been conducted. Codon optimization is a powerful tool for improving the production levels of heterologous proteins. Although codon optimization is generally believed to improve the translation efficiency of heterologous genes without affecting their mRNA levels, several studies have indicated that codon optimization causes an increase in the steady-state mRNA levels of heterologous genes in filamentous fungi. However, the mechanism that determines the low mRNA levels when native heterologous genes are expressed was poorly understood. We recently showed that the transcripts of heterologous genes are polyadenylated prematurely within the coding region and that the heterologous gene transcripts can be stabilized significantly by codon optimization, which is probably attributable to the prevention of premature polyadenylation in Aspergillus oryzae. In this review, we describe the detailed mechanism of premature polyadenylation and the rapid degradation of mRNA transcripts derived from heterologous genes in filamentous fungi. © Springer-Verlag Berlin Heidelberg 2014 |
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Abstract Filamentous fungi, particularly Aspergillus species, have recently attracted attention as host organisms for recombinant protein production. Because the secretory yields of heterologous proteins are generally low compared with those of homologous proteins or proteins from closely related fungal species, several strategies to produce substantial amounts of recombinant proteins have been conducted. Codon optimization is a powerful tool for improving the production levels of heterologous proteins. Although codon optimization is generally believed to improve the translation efficiency of heterologous genes without affecting their mRNA levels, several studies have indicated that codon optimization causes an increase in the steady-state mRNA levels of heterologous genes in filamentous fungi. However, the mechanism that determines the low mRNA levels when native heterologous genes are expressed was poorly understood. We recently showed that the transcripts of heterologous genes are polyadenylated prematurely within the coding region and that the heterologous gene transcripts can be stabilized significantly by codon optimization, which is probably attributable to the prevention of premature polyadenylation in Aspergillus oryzae. In this review, we describe the detailed mechanism of premature polyadenylation and the rapid degradation of mRNA transcripts derived from heterologous genes in filamentous fungi. © Springer-Verlag Berlin Heidelberg 2014 |
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Effects of codon optimization on the mRNA levels of heterologous genes in filamentous fungi |
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