Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells
Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalab...
Ausführliche Beschreibung
Autor*in: |
Frensing, Timo [verfasserIn] |
---|
Format: |
Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2016 |
---|
Schlagwörter: |
---|
Anmerkung: |
© The Author(s) 2016 |
---|
Übergeordnetes Werk: |
Enthalten in: Applied microbiology and biotechnology - Springer Berlin Heidelberg, 1984, 100(2016), 16 vom: 29. Apr., Seite 7181-7192 |
---|---|
Übergeordnetes Werk: |
volume:100 ; year:2016 ; number:16 ; day:29 ; month:04 ; pages:7181-7192 |
Links: |
---|
DOI / URN: |
10.1007/s00253-016-7542-4 |
---|
Katalog-ID: |
OLC2050779127 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | OLC2050779127 | ||
003 | DE-627 | ||
005 | 20230512162814.0 | ||
007 | tu | ||
008 | 200820s2016 xx ||||| 00| ||eng c | ||
024 | 7 | |a 10.1007/s00253-016-7542-4 |2 doi | |
035 | |a (DE-627)OLC2050779127 | ||
035 | |a (DE-He213)s00253-016-7542-4-p | ||
040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
041 | |a eng | ||
082 | 0 | 4 | |a 570 |q VZ |
084 | |a 12 |2 ssgn | ||
084 | |a BIODIV |q DE-30 |2 fid | ||
100 | 1 | |a Frensing, Timo |e verfasserin |4 aut | |
245 | 1 | 0 | |a Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells |
264 | 1 | |c 2016 | |
336 | |a Text |b txt |2 rdacontent | ||
337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
338 | |a Band |b nc |2 rdacarrier | ||
500 | |a © The Author(s) 2016 | ||
520 | |a Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. | ||
650 | 4 | |a Influenza virus | |
650 | 4 | |a Vaccine production | |
650 | 4 | |a Virus release | |
650 | 4 | |a Virus particle morphology | |
700 | 1 | |a Kupke, Sascha Y. |4 aut | |
700 | 1 | |a Bachmann, Mandy |4 aut | |
700 | 1 | |a Fritzsche, Susanne |4 aut | |
700 | 1 | |a Gallo-Ramirez, Lili E. |4 aut | |
700 | 1 | |a Reichl, Udo |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Applied microbiology and biotechnology |d Springer Berlin Heidelberg, 1984 |g 100(2016), 16 vom: 29. Apr., Seite 7181-7192 |w (DE-627)129942634 |w (DE-600)392453-1 |w (DE-576)015507750 |x 0175-7598 |7 nnns |
773 | 1 | 8 | |g volume:100 |g year:2016 |g number:16 |g day:29 |g month:04 |g pages:7181-7192 |
856 | 4 | 1 | |u https://doi.org/10.1007/s00253-016-7542-4 |z lizenzpflichtig |3 Volltext |
912 | |a GBV_USEFLAG_A | ||
912 | |a SYSFLAG_A | ||
912 | |a GBV_OLC | ||
912 | |a FID-BIODIV | ||
912 | |a SSG-OLC-TEC | ||
912 | |a SSG-OLC-CHE | ||
912 | |a SSG-OLC-PHA | ||
912 | |a SSG-OLC-DE-84 | ||
912 | |a GBV_ILN_70 | ||
912 | |a GBV_ILN_130 | ||
912 | |a GBV_ILN_267 | ||
912 | |a GBV_ILN_2018 | ||
912 | |a GBV_ILN_4012 | ||
912 | |a GBV_ILN_4277 | ||
912 | |a GBV_ILN_4305 | ||
951 | |a AR | ||
952 | |d 100 |j 2016 |e 16 |b 29 |c 04 |h 7181-7192 |
author_variant |
t f tf s y k sy syk m b mb s f sf l e g r leg legr u r ur |
---|---|
matchkey_str |
article:01757598:2016----::nlezvrsnrcluarpiainyaiseeskntcadatceopoo |
hierarchy_sort_str |
2016 |
publishDate |
2016 |
allfields |
10.1007/s00253-016-7542-4 doi (DE-627)OLC2050779127 (DE-He213)s00253-016-7542-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Frensing, Timo verfasserin aut Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2016 Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. Influenza virus Vaccine production Virus release Virus particle morphology Kupke, Sascha Y. aut Bachmann, Mandy aut Fritzsche, Susanne aut Gallo-Ramirez, Lili E. aut Reichl, Udo aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 100(2016), 16 vom: 29. Apr., Seite 7181-7192 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:100 year:2016 number:16 day:29 month:04 pages:7181-7192 https://doi.org/10.1007/s00253-016-7542-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4277 GBV_ILN_4305 AR 100 2016 16 29 04 7181-7192 |
spelling |
10.1007/s00253-016-7542-4 doi (DE-627)OLC2050779127 (DE-He213)s00253-016-7542-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Frensing, Timo verfasserin aut Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2016 Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. Influenza virus Vaccine production Virus release Virus particle morphology Kupke, Sascha Y. aut Bachmann, Mandy aut Fritzsche, Susanne aut Gallo-Ramirez, Lili E. aut Reichl, Udo aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 100(2016), 16 vom: 29. Apr., Seite 7181-7192 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:100 year:2016 number:16 day:29 month:04 pages:7181-7192 https://doi.org/10.1007/s00253-016-7542-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4277 GBV_ILN_4305 AR 100 2016 16 29 04 7181-7192 |
allfields_unstemmed |
10.1007/s00253-016-7542-4 doi (DE-627)OLC2050779127 (DE-He213)s00253-016-7542-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Frensing, Timo verfasserin aut Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2016 Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. Influenza virus Vaccine production Virus release Virus particle morphology Kupke, Sascha Y. aut Bachmann, Mandy aut Fritzsche, Susanne aut Gallo-Ramirez, Lili E. aut Reichl, Udo aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 100(2016), 16 vom: 29. Apr., Seite 7181-7192 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:100 year:2016 number:16 day:29 month:04 pages:7181-7192 https://doi.org/10.1007/s00253-016-7542-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4277 GBV_ILN_4305 AR 100 2016 16 29 04 7181-7192 |
allfieldsGer |
10.1007/s00253-016-7542-4 doi (DE-627)OLC2050779127 (DE-He213)s00253-016-7542-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Frensing, Timo verfasserin aut Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2016 Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. Influenza virus Vaccine production Virus release Virus particle morphology Kupke, Sascha Y. aut Bachmann, Mandy aut Fritzsche, Susanne aut Gallo-Ramirez, Lili E. aut Reichl, Udo aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 100(2016), 16 vom: 29. Apr., Seite 7181-7192 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:100 year:2016 number:16 day:29 month:04 pages:7181-7192 https://doi.org/10.1007/s00253-016-7542-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4277 GBV_ILN_4305 AR 100 2016 16 29 04 7181-7192 |
allfieldsSound |
10.1007/s00253-016-7542-4 doi (DE-627)OLC2050779127 (DE-He213)s00253-016-7542-4-p DE-627 ger DE-627 rakwb eng 570 VZ 12 ssgn BIODIV DE-30 fid Frensing, Timo verfasserin aut Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells 2016 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © The Author(s) 2016 Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. Influenza virus Vaccine production Virus release Virus particle morphology Kupke, Sascha Y. aut Bachmann, Mandy aut Fritzsche, Susanne aut Gallo-Ramirez, Lili E. aut Reichl, Udo aut Enthalten in Applied microbiology and biotechnology Springer Berlin Heidelberg, 1984 100(2016), 16 vom: 29. Apr., Seite 7181-7192 (DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 0175-7598 nnns volume:100 year:2016 number:16 day:29 month:04 pages:7181-7192 https://doi.org/10.1007/s00253-016-7542-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4277 GBV_ILN_4305 AR 100 2016 16 29 04 7181-7192 |
language |
English |
source |
Enthalten in Applied microbiology and biotechnology 100(2016), 16 vom: 29. Apr., Seite 7181-7192 volume:100 year:2016 number:16 day:29 month:04 pages:7181-7192 |
sourceStr |
Enthalten in Applied microbiology and biotechnology 100(2016), 16 vom: 29. Apr., Seite 7181-7192 volume:100 year:2016 number:16 day:29 month:04 pages:7181-7192 |
format_phy_str_mv |
Article |
institution |
findex.gbv.de |
topic_facet |
Influenza virus Vaccine production Virus release Virus particle morphology |
dewey-raw |
570 |
isfreeaccess_bool |
false |
container_title |
Applied microbiology and biotechnology |
authorswithroles_txt_mv |
Frensing, Timo @@aut@@ Kupke, Sascha Y. @@aut@@ Bachmann, Mandy @@aut@@ Fritzsche, Susanne @@aut@@ Gallo-Ramirez, Lili E. @@aut@@ Reichl, Udo @@aut@@ |
publishDateDaySort_date |
2016-04-29T00:00:00Z |
hierarchy_top_id |
129942634 |
dewey-sort |
3570 |
id |
OLC2050779127 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">OLC2050779127</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230512162814.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">200820s2016 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00253-016-7542-4</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2050779127</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s00253-016-7542-4-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Frensing, Timo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) 2016</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Influenza virus</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Vaccine production</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Virus release</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Virus particle morphology</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kupke, Sascha Y.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bachmann, Mandy</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fritzsche, Susanne</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gallo-Ramirez, Lili E.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Reichl, Udo</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Applied microbiology and biotechnology</subfield><subfield code="d">Springer Berlin Heidelberg, 1984</subfield><subfield code="g">100(2016), 16 vom: 29. Apr., Seite 7181-7192</subfield><subfield code="w">(DE-627)129942634</subfield><subfield code="w">(DE-600)392453-1</subfield><subfield code="w">(DE-576)015507750</subfield><subfield code="x">0175-7598</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:100</subfield><subfield code="g">year:2016</subfield><subfield code="g">number:16</subfield><subfield code="g">day:29</subfield><subfield code="g">month:04</subfield><subfield code="g">pages:7181-7192</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00253-016-7542-4</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_130</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_267</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">100</subfield><subfield code="j">2016</subfield><subfield code="e">16</subfield><subfield code="b">29</subfield><subfield code="c">04</subfield><subfield code="h">7181-7192</subfield></datafield></record></collection>
|
author |
Frensing, Timo |
spellingShingle |
Frensing, Timo ddc 570 ssgn 12 fid BIODIV misc Influenza virus misc Vaccine production misc Virus release misc Virus particle morphology Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells |
authorStr |
Frensing, Timo |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)129942634 |
format |
Article |
dewey-ones |
570 - Life sciences; biology |
delete_txt_mv |
keep |
author_role |
aut aut aut aut aut aut |
collection |
OLC |
remote_str |
false |
illustrated |
Not Illustrated |
issn |
0175-7598 |
topic_title |
570 VZ 12 ssgn BIODIV DE-30 fid Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells Influenza virus Vaccine production Virus release Virus particle morphology |
topic |
ddc 570 ssgn 12 fid BIODIV misc Influenza virus misc Vaccine production misc Virus release misc Virus particle morphology |
topic_unstemmed |
ddc 570 ssgn 12 fid BIODIV misc Influenza virus misc Vaccine production misc Virus release misc Virus particle morphology |
topic_browse |
ddc 570 ssgn 12 fid BIODIV misc Influenza virus misc Vaccine production misc Virus release misc Virus particle morphology |
format_facet |
Aufsätze Gedruckte Aufsätze |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
nc |
hierarchy_parent_title |
Applied microbiology and biotechnology |
hierarchy_parent_id |
129942634 |
dewey-tens |
570 - Life sciences; biology |
hierarchy_top_title |
Applied microbiology and biotechnology |
isfreeaccess_txt |
false |
familylinks_str_mv |
(DE-627)129942634 (DE-600)392453-1 (DE-576)015507750 |
title |
Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells |
ctrlnum |
(DE-627)OLC2050779127 (DE-He213)s00253-016-7542-4-p |
title_full |
Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells |
author_sort |
Frensing, Timo |
journal |
Applied microbiology and biotechnology |
journalStr |
Applied microbiology and biotechnology |
lang_code |
eng |
isOA_bool |
false |
dewey-hundreds |
500 - Science |
recordtype |
marc |
publishDateSort |
2016 |
contenttype_str_mv |
txt |
container_start_page |
7181 |
author_browse |
Frensing, Timo Kupke, Sascha Y. Bachmann, Mandy Fritzsche, Susanne Gallo-Ramirez, Lili E. Reichl, Udo |
container_volume |
100 |
class |
570 VZ 12 ssgn BIODIV DE-30 fid |
format_se |
Aufsätze |
author-letter |
Frensing, Timo |
doi_str_mv |
10.1007/s00253-016-7542-4 |
dewey-full |
570 |
title_sort |
influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in mdck cells |
title_auth |
Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells |
abstract |
Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. © The Author(s) 2016 |
abstractGer |
Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. © The Author(s) 2016 |
abstract_unstemmed |
Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required. © The Author(s) 2016 |
collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-TEC SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_130 GBV_ILN_267 GBV_ILN_2018 GBV_ILN_4012 GBV_ILN_4277 GBV_ILN_4305 |
container_issue |
16 |
title_short |
Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells |
url |
https://doi.org/10.1007/s00253-016-7542-4 |
remote_bool |
false |
author2 |
Kupke, Sascha Y. Bachmann, Mandy Fritzsche, Susanne Gallo-Ramirez, Lili E. Reichl, Udo |
author2Str |
Kupke, Sascha Y. Bachmann, Mandy Fritzsche, Susanne Gallo-Ramirez, Lili E. Reichl, Udo |
ppnlink |
129942634 |
mediatype_str_mv |
n |
isOA_txt |
false |
hochschulschrift_bool |
false |
doi_str |
10.1007/s00253-016-7542-4 |
up_date |
2024-07-04T02:51:17.058Z |
_version_ |
1803615183377006592 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">OLC2050779127</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230512162814.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">200820s2016 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s00253-016-7542-4</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2050779127</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s00253-016-7542-4-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Frensing, Timo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Influenza virus intracellular replication dynamics, release kinetics, and particle morphology during propagation in MDCK cells</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) 2016</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Influenza viruses are respiratory pathogens and can cause severe disease. The best protection against influenza is provided by annual vaccination. These vaccines are produced in embryonated chicken eggs or using continuous animal cell lines. The latter processes are more flexible and scalable to meet the growing global demand. However, virus production in cell cultures is more expensive. Hence, further research is needed to make these processes more cost-effective and robust. We studied influenza virus replication dynamics to identify factors that limit the virus yield in adherent Madin-Darby canine kidney (MDCK) cells. The cell cycle stage of MDCK cells had no impact during early infection. Yet, our results showed that the influenza virus RNA synthesis levels out already 4 h post infection at a time when viral genome segments are exported from the nucleus. Nevertheless, virus release occurred at a constant rate in the following 16 h. Thereafter, the production of infectious viruses dramatically decreased, but cells continued to produce particles contributing to the hemagglutination (HA) titer. The majority of these particles from the late phase of infection were deformed or broken virus particles as well as large membranous structures decorated with viral surface proteins. These changes in particle characteristics and morphology need to be considered for the optimization of influenza virus production and vaccine purification steps. Moreover, our data suggest that in order to achieve higher cell-specific yields, a prolonged phase of viral RNA synthesis and/or a more efficient release of influenza virus particles is required.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Influenza virus</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Vaccine production</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Virus release</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Virus particle morphology</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kupke, Sascha Y.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bachmann, Mandy</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Fritzsche, Susanne</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gallo-Ramirez, Lili E.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Reichl, Udo</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Applied microbiology and biotechnology</subfield><subfield code="d">Springer Berlin Heidelberg, 1984</subfield><subfield code="g">100(2016), 16 vom: 29. Apr., Seite 7181-7192</subfield><subfield code="w">(DE-627)129942634</subfield><subfield code="w">(DE-600)392453-1</subfield><subfield code="w">(DE-576)015507750</subfield><subfield code="x">0175-7598</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:100</subfield><subfield code="g">year:2016</subfield><subfield code="g">number:16</subfield><subfield code="g">day:29</subfield><subfield code="g">month:04</subfield><subfield code="g">pages:7181-7192</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s00253-016-7542-4</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-CHE</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_130</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_267</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2018</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4277</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">100</subfield><subfield code="j">2016</subfield><subfield code="e">16</subfield><subfield code="b">29</subfield><subfield code="c">04</subfield><subfield code="h">7181-7192</subfield></datafield></record></collection>
|
score |
7.399479 |