High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation
Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by co...
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Gespeichert in:
| Autor*in: |
Jain, Pankaj Kumar [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Anmerkung: |
© Akadémiai Kiadó, Budapest, Hungary 2020 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of planar chromatography, modern TLC - Springer International Publishing, 1988, 33(2020), 2 vom: Apr., Seite 191-201 |
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| Übergeordnetes Werk: |
volume:33 ; year:2020 ; number:2 ; month:04 ; pages:191-201 |
| Links: |
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| DOI / URN: |
10.1007/s00764-020-00016-1 |
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| Katalog-ID: |
OLC2051476160 |
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| 520 | |a Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract | ||
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10.1007/s00764-020-00016-1 doi (DE-627)OLC2051476160 (DE-He213)s00764-020-00016-1-p DE-627 ger DE-627 rakwb eng 540 530 VZ Jain, Pankaj Kumar verfasserin aut High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Akadémiai Kiadó, Budapest, Hungary 2020 Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract High-performance thin-layer chromatography Total phenolic content Total flavonoid content Jain, Sonika aut Chak, Pooja aut Swarnkar, Surendra Kumar aut Sharma, Swapnil aut Paliwal, Sarvesh aut Enthalten in Journal of planar chromatography, modern TLC Springer International Publishing, 1988 33(2020), 2 vom: Apr., Seite 191-201 (DE-627)129250651 (DE-600)59576-7 (DE-576)017944201 0933-4173 nnns volume:33 year:2020 number:2 month:04 pages:191-201 https://doi.org/10.1007/s00764-020-00016-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_2129 AR 33 2020 2 04 191-201 |
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10.1007/s00764-020-00016-1 doi (DE-627)OLC2051476160 (DE-He213)s00764-020-00016-1-p DE-627 ger DE-627 rakwb eng 540 530 VZ Jain, Pankaj Kumar verfasserin aut High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Akadémiai Kiadó, Budapest, Hungary 2020 Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract High-performance thin-layer chromatography Total phenolic content Total flavonoid content Jain, Sonika aut Chak, Pooja aut Swarnkar, Surendra Kumar aut Sharma, Swapnil aut Paliwal, Sarvesh aut Enthalten in Journal of planar chromatography, modern TLC Springer International Publishing, 1988 33(2020), 2 vom: Apr., Seite 191-201 (DE-627)129250651 (DE-600)59576-7 (DE-576)017944201 0933-4173 nnns volume:33 year:2020 number:2 month:04 pages:191-201 https://doi.org/10.1007/s00764-020-00016-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_2129 AR 33 2020 2 04 191-201 |
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10.1007/s00764-020-00016-1 doi (DE-627)OLC2051476160 (DE-He213)s00764-020-00016-1-p DE-627 ger DE-627 rakwb eng 540 530 VZ Jain, Pankaj Kumar verfasserin aut High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Akadémiai Kiadó, Budapest, Hungary 2020 Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract High-performance thin-layer chromatography Total phenolic content Total flavonoid content Jain, Sonika aut Chak, Pooja aut Swarnkar, Surendra Kumar aut Sharma, Swapnil aut Paliwal, Sarvesh aut Enthalten in Journal of planar chromatography, modern TLC Springer International Publishing, 1988 33(2020), 2 vom: Apr., Seite 191-201 (DE-627)129250651 (DE-600)59576-7 (DE-576)017944201 0933-4173 nnns volume:33 year:2020 number:2 month:04 pages:191-201 https://doi.org/10.1007/s00764-020-00016-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_2129 AR 33 2020 2 04 191-201 |
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10.1007/s00764-020-00016-1 doi (DE-627)OLC2051476160 (DE-He213)s00764-020-00016-1-p DE-627 ger DE-627 rakwb eng 540 530 VZ Jain, Pankaj Kumar verfasserin aut High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Akadémiai Kiadó, Budapest, Hungary 2020 Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract High-performance thin-layer chromatography Total phenolic content Total flavonoid content Jain, Sonika aut Chak, Pooja aut Swarnkar, Surendra Kumar aut Sharma, Swapnil aut Paliwal, Sarvesh aut Enthalten in Journal of planar chromatography, modern TLC Springer International Publishing, 1988 33(2020), 2 vom: Apr., Seite 191-201 (DE-627)129250651 (DE-600)59576-7 (DE-576)017944201 0933-4173 nnns volume:33 year:2020 number:2 month:04 pages:191-201 https://doi.org/10.1007/s00764-020-00016-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_2129 AR 33 2020 2 04 191-201 |
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10.1007/s00764-020-00016-1 doi (DE-627)OLC2051476160 (DE-He213)s00764-020-00016-1-p DE-627 ger DE-627 rakwb eng 540 530 VZ Jain, Pankaj Kumar verfasserin aut High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation 2020 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Akadémiai Kiadó, Budapest, Hungary 2020 Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract High-performance thin-layer chromatography Total phenolic content Total flavonoid content Jain, Sonika aut Chak, Pooja aut Swarnkar, Surendra Kumar aut Sharma, Swapnil aut Paliwal, Sarvesh aut Enthalten in Journal of planar chromatography, modern TLC Springer International Publishing, 1988 33(2020), 2 vom: Apr., Seite 191-201 (DE-627)129250651 (DE-600)59576-7 (DE-576)017944201 0933-4173 nnns volume:33 year:2020 number:2 month:04 pages:191-201 https://doi.org/10.1007/s00764-020-00016-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-PHY SSG-OLC-CHE SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_70 GBV_ILN_2129 AR 33 2020 2 04 191-201 |
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Journal of planar chromatography, modern TLC |
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Jain, Pankaj Kumar Jain, Sonika Chak, Pooja Swarnkar, Surendra Kumar Sharma, Swapnil Paliwal, Sarvesh |
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high-performance thin-layer chromatographic investigation of rutin in the leaves of phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation |
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High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation |
| abstract |
Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract © Akadémiai Kiadó, Budapest, Hungary 2020 |
| abstractGer |
Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract © Akadémiai Kiadó, Budapest, Hungary 2020 |
| abstract_unstemmed |
Abstract The current research aimed to investigate the pharmacognostical, phytochemical and quantification of rutin in leaves of Phoenix sylvestris by high-performance thin-layer chromatographic (HPTLC) method. Various extracts of the leaves of P. sylvestris were prepared in different solvents by cold maceration method. The extracts were subjected to various chemical tests for detection of various secondary metabolites as well as to determine the amount of total phenolic content (TPC) and total flavonoids content (TFC). The methanolic extract was subjected to carry out quantification of rutin by HPTLC method. The method was validated for linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), etc. Pharmacognostic study mainly covered the macroscopic and microscopic features of the leaves including powder microscopy. Qualitative analysis revealed the existence of certain chemical constituents such as flavonoids, tannins, organic acids and saponin glycosides. The methanolic extract of P. sylvestris showed LOD and LOQ for rutin as 125.01 ng/spot and 204.921 ng/spot, respectively. The amount of rutin was detected as 54.6 mg $ g^{-1} $ of extract. Graphical abstract © Akadémiai Kiadó, Budapest, Hungary 2020 |
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High-performance thin-layer chromatographic investigation of rutin in the leaves of Phoenix sylvestris in sequence with pharmacognostical and phytochemical evaluation |
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