Investigation of the Effect of Various Buffer Solutions Used for Microinjection of Gene-Engineering Constructs on Preimplantation Development of Mouse Embryos
Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and bla...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Popova, E. A. [verfasserIn] |
|---|
| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2002 |
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| Anmerkung: |
© MAIK “Nauka/Interperiodica” 2002 |
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| Übergeordnetes Werk: |
Enthalten in: Russian journal of developmental biology - Kluwer Academic Publishers-Plenum Publishers, 1992, 33(2002), 2 vom: März, Seite 110-112 |
|---|---|
| Übergeordnetes Werk: |
volume:33 ; year:2002 ; number:2 ; month:03 ; pages:110-112 |
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| DOI / URN: |
10.1023/A:1014924431036 |
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OLC2061976077 |
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| 520 | |a Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions. | ||
| 700 | 1 | |a Krivokharchenko, A. S. |4 aut | |
| 700 | 1 | |a Vil'yanovich, L. I. |4 aut | |
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10.1023/A:1014924431036 doi (DE-627)OLC2061976077 (DE-He213)A:1014924431036-p DE-627 ger DE-627 rakwb eng 590 570 VZ 12 ssgn BIODIV DE-30 fid Popova, E. A. verfasserin aut Investigation of the Effect of Various Buffer Solutions Used for Microinjection of Gene-Engineering Constructs on Preimplantation Development of Mouse Embryos 2002 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © MAIK “Nauka/Interperiodica” 2002 Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions. Krivokharchenko, A. S. aut Vil'yanovich, L. I. aut Enthalten in Russian journal of developmental biology Kluwer Academic Publishers-Plenum Publishers, 1992 33(2002), 2 vom: März, Seite 110-112 (DE-627)165920963 (DE-600)33904-0 (DE-576)515684872 1062-3604 nnns volume:33 year:2002 number:2 month:03 pages:110-112 https://doi.org/10.1023/A:1014924431036 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-OEU GBV_ILN_4082 AR 33 2002 2 03 110-112 |
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10.1023/A:1014924431036 doi (DE-627)OLC2061976077 (DE-He213)A:1014924431036-p DE-627 ger DE-627 rakwb eng 590 570 VZ 12 ssgn BIODIV DE-30 fid Popova, E. A. verfasserin aut Investigation of the Effect of Various Buffer Solutions Used for Microinjection of Gene-Engineering Constructs on Preimplantation Development of Mouse Embryos 2002 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © MAIK “Nauka/Interperiodica” 2002 Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions. Krivokharchenko, A. S. aut Vil'yanovich, L. I. aut Enthalten in Russian journal of developmental biology Kluwer Academic Publishers-Plenum Publishers, 1992 33(2002), 2 vom: März, Seite 110-112 (DE-627)165920963 (DE-600)33904-0 (DE-576)515684872 1062-3604 nnns volume:33 year:2002 number:2 month:03 pages:110-112 https://doi.org/10.1023/A:1014924431036 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-OEU GBV_ILN_4082 AR 33 2002 2 03 110-112 |
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10.1023/A:1014924431036 doi (DE-627)OLC2061976077 (DE-He213)A:1014924431036-p DE-627 ger DE-627 rakwb eng 590 570 VZ 12 ssgn BIODIV DE-30 fid Popova, E. A. verfasserin aut Investigation of the Effect of Various Buffer Solutions Used for Microinjection of Gene-Engineering Constructs on Preimplantation Development of Mouse Embryos 2002 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © MAIK “Nauka/Interperiodica” 2002 Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions. Krivokharchenko, A. S. aut Vil'yanovich, L. I. aut Enthalten in Russian journal of developmental biology Kluwer Academic Publishers-Plenum Publishers, 1992 33(2002), 2 vom: März, Seite 110-112 (DE-627)165920963 (DE-600)33904-0 (DE-576)515684872 1062-3604 nnns volume:33 year:2002 number:2 month:03 pages:110-112 https://doi.org/10.1023/A:1014924431036 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-OEU GBV_ILN_4082 AR 33 2002 2 03 110-112 |
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10.1023/A:1014924431036 doi (DE-627)OLC2061976077 (DE-He213)A:1014924431036-p DE-627 ger DE-627 rakwb eng 590 570 VZ 12 ssgn BIODIV DE-30 fid Popova, E. A. verfasserin aut Investigation of the Effect of Various Buffer Solutions Used for Microinjection of Gene-Engineering Constructs on Preimplantation Development of Mouse Embryos 2002 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © MAIK “Nauka/Interperiodica” 2002 Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions. Krivokharchenko, A. S. aut Vil'yanovich, L. I. aut Enthalten in Russian journal of developmental biology Kluwer Academic Publishers-Plenum Publishers, 1992 33(2002), 2 vom: März, Seite 110-112 (DE-627)165920963 (DE-600)33904-0 (DE-576)515684872 1062-3604 nnns volume:33 year:2002 number:2 month:03 pages:110-112 https://doi.org/10.1023/A:1014924431036 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC FID-BIODIV SSG-OLC-OEU GBV_ILN_4082 AR 33 2002 2 03 110-112 |
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Investigation of the Effect of Various Buffer Solutions Used for Microinjection of Gene-Engineering Constructs on Preimplantation Development of Mouse Embryos |
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Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions. © MAIK “Nauka/Interperiodica” 2002 |
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Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions. © MAIK “Nauka/Interperiodica” 2002 |
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Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions. © MAIK “Nauka/Interperiodica” 2002 |
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2024-07-04T04:53:12.454Z |
| _version_ |
1803622854106808320 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">OLC2061976077</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230504032224.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">200820s2002 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1023/A:1014924431036</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2061976077</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)A:1014924431036-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">590</subfield><subfield code="a">570</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">12</subfield><subfield code="2">ssgn</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Popova, E. A.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Investigation of the Effect of Various Buffer Solutions Used for Microinjection of Gene-Engineering Constructs on Preimplantation Development of Mouse Embryos</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2002</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© MAIK “Nauka/Interperiodica” 2002</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract We studied the effects of some buffer solutions used for microinjection in mammalian zygotes on preimplantation development of (CBA × C57BL) $ F_{1} $ mouse embryos in vitro. The rate of embryo survival was estimated according to their capacity to develop to the stages of blastocyst and blastocyst hatched from zona pellucida. The results obtained suggested a reduced rate of survival of zygotes to the blastocyst stage after the injection into a pronucleus of the buffers Tris-HCl with EDTA, Tris-HCl with $ MgCl_{2} $ and NaCl, and medium M2 (p < 0.05) and to the stage of blastocyst hatched from zone pellucida after injection of a Dulbecco solution, as compared to the control. No differences were found in the survival rate of zygotes injected with different buffer solutions.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Krivokharchenko, A. S.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Vil'yanovich, L. I.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Russian journal of developmental biology</subfield><subfield code="d">Kluwer Academic Publishers-Plenum Publishers, 1992</subfield><subfield code="g">33(2002), 2 vom: März, Seite 110-112</subfield><subfield code="w">(DE-627)165920963</subfield><subfield code="w">(DE-600)33904-0</subfield><subfield code="w">(DE-576)515684872</subfield><subfield code="x">1062-3604</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:33</subfield><subfield code="g">year:2002</subfield><subfield code="g">number:2</subfield><subfield code="g">month:03</subfield><subfield code="g">pages:110-112</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1023/A:1014924431036</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-OEU</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4082</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">33</subfield><subfield code="j">2002</subfield><subfield code="e">2</subfield><subfield code="c">03</subfield><subfield code="h">110-112</subfield></datafield></record></collection>
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