Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics
Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetra...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Riley, Susan L. [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2001 |
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| Schlagwörter: |
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| Anmerkung: |
© Kluwer Academic Publishers 2001 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of materials science / Materials in medicine - Kluwer Academic Publishers, 1990, 12(2001), 10-12 vom: Dez., Seite 983-990 |
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| Übergeordnetes Werk: |
volume:12 ; year:2001 ; number:10-12 ; month:12 ; pages:983-990 |
| Links: |
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| DOI / URN: |
10.1023/A:1012817317296 |
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| Katalog-ID: |
OLC2066794031 |
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| 520 | |a Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers | ||
| 650 | 4 | |a Ethylene Oxide | |
| 650 | 4 | |a Cartilage Tissue | |
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| 700 | 1 | |a Dutt, Sangeeta |4 aut | |
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| 700 | 1 | |a Chen, Albert C. |4 aut | |
| 700 | 1 | |a Sah, Robert L. |4 aut | |
| 700 | 1 | |a Ratcliffe, Anthony |4 aut | |
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10.1023/A:1012817317296 doi (DE-627)OLC2066794031 (DE-He213)A:1012817317296-p DE-627 ger DE-627 rakwb eng 610 670 VZ Riley, Susan L. verfasserin aut Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics 2001 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Kluwer Academic Publishers 2001 Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers Ethylene Oxide Cartilage Tissue Compressive Modulus Cartilage Tissue Engineering Crosslinkable Poly Dutt, Sangeeta aut de la Torre, Rebecca aut Chen, Albert C. aut Sah, Robert L. aut Ratcliffe, Anthony aut Enthalten in Journal of materials science / Materials in medicine Kluwer Academic Publishers, 1990 12(2001), 10-12 vom: Dez., Seite 983-990 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:12 year:2001 number:10-12 month:12 pages:983-990 https://doi.org/10.1023/A:1012817317296 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4323 AR 12 2001 10-12 12 983-990 |
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10.1023/A:1012817317296 doi (DE-627)OLC2066794031 (DE-He213)A:1012817317296-p DE-627 ger DE-627 rakwb eng 610 670 VZ Riley, Susan L. verfasserin aut Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics 2001 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Kluwer Academic Publishers 2001 Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers Ethylene Oxide Cartilage Tissue Compressive Modulus Cartilage Tissue Engineering Crosslinkable Poly Dutt, Sangeeta aut de la Torre, Rebecca aut Chen, Albert C. aut Sah, Robert L. aut Ratcliffe, Anthony aut Enthalten in Journal of materials science / Materials in medicine Kluwer Academic Publishers, 1990 12(2001), 10-12 vom: Dez., Seite 983-990 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:12 year:2001 number:10-12 month:12 pages:983-990 https://doi.org/10.1023/A:1012817317296 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4323 AR 12 2001 10-12 12 983-990 |
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10.1023/A:1012817317296 doi (DE-627)OLC2066794031 (DE-He213)A:1012817317296-p DE-627 ger DE-627 rakwb eng 610 670 VZ Riley, Susan L. verfasserin aut Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics 2001 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Kluwer Academic Publishers 2001 Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers Ethylene Oxide Cartilage Tissue Compressive Modulus Cartilage Tissue Engineering Crosslinkable Poly Dutt, Sangeeta aut de la Torre, Rebecca aut Chen, Albert C. aut Sah, Robert L. aut Ratcliffe, Anthony aut Enthalten in Journal of materials science / Materials in medicine Kluwer Academic Publishers, 1990 12(2001), 10-12 vom: Dez., Seite 983-990 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:12 year:2001 number:10-12 month:12 pages:983-990 https://doi.org/10.1023/A:1012817317296 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4323 AR 12 2001 10-12 12 983-990 |
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10.1023/A:1012817317296 doi (DE-627)OLC2066794031 (DE-He213)A:1012817317296-p DE-627 ger DE-627 rakwb eng 610 670 VZ Riley, Susan L. verfasserin aut Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics 2001 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Kluwer Academic Publishers 2001 Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers Ethylene Oxide Cartilage Tissue Compressive Modulus Cartilage Tissue Engineering Crosslinkable Poly Dutt, Sangeeta aut de la Torre, Rebecca aut Chen, Albert C. aut Sah, Robert L. aut Ratcliffe, Anthony aut Enthalten in Journal of materials science / Materials in medicine Kluwer Academic Publishers, 1990 12(2001), 10-12 vom: Dez., Seite 983-990 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:12 year:2001 number:10-12 month:12 pages:983-990 https://doi.org/10.1023/A:1012817317296 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4323 AR 12 2001 10-12 12 983-990 |
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10.1023/A:1012817317296 doi (DE-627)OLC2066794031 (DE-He213)A:1012817317296-p DE-627 ger DE-627 rakwb eng 610 670 VZ Riley, Susan L. verfasserin aut Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics 2001 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Kluwer Academic Publishers 2001 Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers Ethylene Oxide Cartilage Tissue Compressive Modulus Cartilage Tissue Engineering Crosslinkable Poly Dutt, Sangeeta aut de la Torre, Rebecca aut Chen, Albert C. aut Sah, Robert L. aut Ratcliffe, Anthony aut Enthalten in Journal of materials science / Materials in medicine Kluwer Academic Publishers, 1990 12(2001), 10-12 vom: Dez., Seite 983-990 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:12 year:2001 number:10-12 month:12 pages:983-990 https://doi.org/10.1023/A:1012817317296 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_11 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_65 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4323 AR 12 2001 10-12 12 983-990 |
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formulation of peg-based hydrogels affects tissue-engineered cartilage construct characteristics |
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Formulation of PEG-based hydrogels affects tissue-engineered cartilage construct characteristics |
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Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers © Kluwer Academic Publishers 2001 |
| abstractGer |
Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers © Kluwer Academic Publishers 2001 |
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Abstract The limited supply of cartilage tissue with appropriate sizes and shapes needed for reconstruction and repair has stimulated research in the area of hydrogels as scaffolds for cartilage tissue engineering. In this study we demonstrate that poly(ethylene glycol) (PEG)-based semi-interpenetrating (sIPN) network hydrogels, made with a crosslinkable poly(ethylene glycol)-dimethacrylate (PEGDM) component and a non-crosslinkable interpenetration poly(ethylene oxide) (PEO) component, and seeded with chondrocytes support cartilage construct growth having nominal thicknesses of 6 mm and relatively uniform safranin-O stained matrix when cultured statically, unlike constructs grown with prefabricated macroporous scaffolds. Even though changing the molecular weight of the PEO from 100 to 20 kDa reduces the viscosity of the precursor polymer solution, we have demonstrated that it does not appear to affect the histological or biochemical characteristics of cartilaginous constructs. Extracellular matrix (ECM) accumulation and the spatial uniformity of the ECM deposited by the embedded chondrocytes decreased, and hydrogel compressive properties increased, as the ratio of the PEGDM:PEO in the hydrogel formulation increased (from 30:70 to 100:0 PEGDM:PEO). Total collagen and glycosaminoglycan contents per dry weight were highest using the 30:70 PEGDM:PEO formulation (24.4±3.5% and 7.1±0.9%, respectively). The highest equilibrium compressive modulus was obtained using the 100:0 PEGDM:PEO formulation (0.32±0.07 MPa), which is similar to the compressive modulus of native articular cartilage. These results suggest that the versatility of PEG-based sIPN hydrogels makes them an attractive scaffold for tissue engineering of cartilage. © 2001 Kluwer Academic Publishers © Kluwer Academic Publishers 2001 |
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