Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations
Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact tha...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Curran, Judith M. [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2009 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC 2009 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of materials science / Materials in medicine - Springer US, 1990, 21(2009), 3 vom: 27. Dez., Seite 1021-1029 |
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| Übergeordnetes Werk: |
volume:21 ; year:2009 ; number:3 ; day:27 ; month:12 ; pages:1021-1029 |
| Links: |
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| DOI / URN: |
10.1007/s10856-009-3976-x |
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OLC2066813613 |
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| 520 | |a Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. | ||
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| 650 | 4 | |a Spatial Stimulus | |
| 650 | 4 | |a Mesenchymal Stem Cell Differentiation | |
| 650 | 4 | |a Initial Cell Adhesion | |
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| 700 | 1 | |a Chen, Rui |4 aut | |
| 700 | 1 | |a Stokes, Robert |4 aut | |
| 700 | 1 | |a Irvine, Eleanor |4 aut | |
| 700 | 1 | |a Graham, Duncan |4 aut | |
| 700 | 1 | |a Gubbins, Earl |4 aut | |
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| 700 | 1 | |a Amro, Nabil |4 aut | |
| 700 | 1 | |a Sanedrin, Raymond |4 aut | |
| 700 | 1 | |a Jamil, Haris |4 aut | |
| 700 | 1 | |a Hunt, John A. |4 aut | |
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10.1007/s10856-009-3976-x doi (DE-627)OLC2066813613 (DE-He213)s10856-009-3976-x-p DE-627 ger DE-627 rakwb eng 610 670 VZ Curran, Judith M. verfasserin aut Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2009 Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. Mesenchymal Stem Cell Spatial Stimulus Mesenchymal Stem Cell Differentiation Initial Cell Adhesion Mesenchymal Stem Cell Population Chen, Rui aut Stokes, Robert aut Irvine, Eleanor aut Graham, Duncan aut Gubbins, Earl aut Delaney, Deany aut Amro, Nabil aut Sanedrin, Raymond aut Jamil, Haris aut Hunt, John A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 21(2009), 3 vom: 27. Dez., Seite 1021-1029 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:21 year:2009 number:3 day:27 month:12 pages:1021-1029 https://doi.org/10.1007/s10856-009-3976-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 21 2009 3 27 12 1021-1029 |
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10.1007/s10856-009-3976-x doi (DE-627)OLC2066813613 (DE-He213)s10856-009-3976-x-p DE-627 ger DE-627 rakwb eng 610 670 VZ Curran, Judith M. verfasserin aut Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2009 Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. Mesenchymal Stem Cell Spatial Stimulus Mesenchymal Stem Cell Differentiation Initial Cell Adhesion Mesenchymal Stem Cell Population Chen, Rui aut Stokes, Robert aut Irvine, Eleanor aut Graham, Duncan aut Gubbins, Earl aut Delaney, Deany aut Amro, Nabil aut Sanedrin, Raymond aut Jamil, Haris aut Hunt, John A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 21(2009), 3 vom: 27. Dez., Seite 1021-1029 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:21 year:2009 number:3 day:27 month:12 pages:1021-1029 https://doi.org/10.1007/s10856-009-3976-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 21 2009 3 27 12 1021-1029 |
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10.1007/s10856-009-3976-x doi (DE-627)OLC2066813613 (DE-He213)s10856-009-3976-x-p DE-627 ger DE-627 rakwb eng 610 670 VZ Curran, Judith M. verfasserin aut Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2009 Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. Mesenchymal Stem Cell Spatial Stimulus Mesenchymal Stem Cell Differentiation Initial Cell Adhesion Mesenchymal Stem Cell Population Chen, Rui aut Stokes, Robert aut Irvine, Eleanor aut Graham, Duncan aut Gubbins, Earl aut Delaney, Deany aut Amro, Nabil aut Sanedrin, Raymond aut Jamil, Haris aut Hunt, John A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 21(2009), 3 vom: 27. Dez., Seite 1021-1029 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:21 year:2009 number:3 day:27 month:12 pages:1021-1029 https://doi.org/10.1007/s10856-009-3976-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 21 2009 3 27 12 1021-1029 |
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10.1007/s10856-009-3976-x doi (DE-627)OLC2066813613 (DE-He213)s10856-009-3976-x-p DE-627 ger DE-627 rakwb eng 610 670 VZ Curran, Judith M. verfasserin aut Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2009 Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. Mesenchymal Stem Cell Spatial Stimulus Mesenchymal Stem Cell Differentiation Initial Cell Adhesion Mesenchymal Stem Cell Population Chen, Rui aut Stokes, Robert aut Irvine, Eleanor aut Graham, Duncan aut Gubbins, Earl aut Delaney, Deany aut Amro, Nabil aut Sanedrin, Raymond aut Jamil, Haris aut Hunt, John A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 21(2009), 3 vom: 27. Dez., Seite 1021-1029 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:21 year:2009 number:3 day:27 month:12 pages:1021-1029 https://doi.org/10.1007/s10856-009-3976-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 21 2009 3 27 12 1021-1029 |
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10.1007/s10856-009-3976-x doi (DE-627)OLC2066813613 (DE-He213)s10856-009-3976-x-p DE-627 ger DE-627 rakwb eng 610 670 VZ Curran, Judith M. verfasserin aut Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations 2009 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2009 Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. Mesenchymal Stem Cell Spatial Stimulus Mesenchymal Stem Cell Differentiation Initial Cell Adhesion Mesenchymal Stem Cell Population Chen, Rui aut Stokes, Robert aut Irvine, Eleanor aut Graham, Duncan aut Gubbins, Earl aut Delaney, Deany aut Amro, Nabil aut Sanedrin, Raymond aut Jamil, Haris aut Hunt, John A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 21(2009), 3 vom: 27. Dez., Seite 1021-1029 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:21 year:2009 number:3 day:27 month:12 pages:1021-1029 https://doi.org/10.1007/s10856-009-3976-x lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 21 2009 3 27 12 1021-1029 |
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Curran, Judith M. @@aut@@ Chen, Rui @@aut@@ Stokes, Robert @@aut@@ Irvine, Eleanor @@aut@@ Graham, Duncan @@aut@@ Gubbins, Earl @@aut@@ Delaney, Deany @@aut@@ Amro, Nabil @@aut@@ Sanedrin, Raymond @@aut@@ Jamil, Haris @@aut@@ Hunt, John A. @@aut@@ |
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Curran, Judith M. |
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nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations |
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Nanoscale definition of substrate materials to direct human adult stem cells towards tissue specific populations |
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Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. © Springer Science+Business Media, LLC 2009 |
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Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. © Springer Science+Business Media, LLC 2009 |
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Abstract The development of homogenously nano-patterned chemically modified surfaces that can be used to initiate a cellular response, particularly stem cell differentiation, in a highly controlled manner without the need for exogenous biological factors has never been reported, due to that fact that precisely defined and reproducible systems have not been available that can be used to study cell/material interactions and unlock the potential of a material driven cell response. Until now material driven stem cell (furthermore any cell) responses have been variable due to the limitations in definition and reproducibility of the underlying substrate and the lack of true homogeneity of modifications that can dictate a cellular response at a sub-micron level that can effectively control initial cell interactions of all cells that contact the surface. Here we report the successful design and use of homogenously molecularly nanopatterned surfaces to control initial stem cell adhesion and hence function. The highly specified nano-patterned arrays were compared directly to silane modified bulk coated substrates that have previously been proven to initiate mesenchymal stem cell (MSC) differentiation in a heterogenous manner, the aim of this study was to prove the efficiency of these previously observed cell responses could be enhanced by the incorporation of nano-patterns. Nano-patterned surfaces were prepared by Dip Pen $ Nanolithography^{®} $ ($ DPN^{®} $) to produce arrays of 70 nm sized dots separated by defined spacings of 140, 280 and 1000 nm with terminal functionalities of carboxyl, amino, methyl and hydroxyl and used to control cell growth. These nanopatterned surfaces exhibited unprecedented control of initial cell interactions and will change the capabilities for stem cell definition in vitro and then cell based medical therapies. In addition to highlighting the ability of the materials to control stem cell functionality on an unprecedented scale this research also introduces the successful scale-up of $ DPN^{®} $ and the novel chemistries and systems to facilitate the production of homogeneously patterned substrates (5 $ mm^{2} $) that are applicable for use in in vitro cell conditions over prolonged periods for complete control of material driven cell responses. © Springer Science+Business Media, LLC 2009 |
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