Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor
Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dime...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wu, J. M. [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2010 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC 2010 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of materials science / Materials in medicine - Springer US, 1990, 22(2010), 1 vom: 04. Nov., Seite 107-114 |
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| Übergeordnetes Werk: |
volume:22 ; year:2010 ; number:1 ; day:04 ; month:11 ; pages:107-114 |
| Links: |
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| DOI / URN: |
10.1007/s10856-010-4176-4 |
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| Katalog-ID: |
OLC2066816043 |
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| 520 | |a Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. | ||
| 650 | 4 | |a Collagen matrix | |
| 650 | 4 | |a Basic fibroblast growth factor | |
| 650 | 4 | |a Heparin | |
| 650 | 4 | |a Crosslinking | |
| 650 | 4 | |a Controlled release | |
| 700 | 1 | |a Xu, Y. Y. |4 aut | |
| 700 | 1 | |a Li, Z. H. |4 aut | |
| 700 | 1 | |a Yuan, X. Y. |4 aut | |
| 700 | 1 | |a Wang, P. F. |4 aut | |
| 700 | 1 | |a Zhang, X. Z. |4 aut | |
| 700 | 1 | |a Liu, Y. Q. |4 aut | |
| 700 | 1 | |a Guan, J. |4 aut | |
| 700 | 1 | |a Guo, Y. |4 aut | |
| 700 | 1 | |a Li, R. X. |4 aut | |
| 700 | 1 | |a Zhang, H. |4 aut | |
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10.1007/s10856-010-4176-4 doi (DE-627)OLC2066816043 (DE-He213)s10856-010-4176-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Wu, J. M. verfasserin aut Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2010 Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. Collagen matrix Basic fibroblast growth factor Heparin Crosslinking Controlled release Xu, Y. Y. aut Li, Z. H. aut Yuan, X. Y. aut Wang, P. F. aut Zhang, X. Z. aut Liu, Y. Q. aut Guan, J. aut Guo, Y. aut Li, R. X. aut Zhang, H. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2010), 1 vom: 04. Nov., Seite 107-114 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2010 number:1 day:04 month:11 pages:107-114 https://doi.org/10.1007/s10856-010-4176-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2010 1 04 11 107-114 |
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10.1007/s10856-010-4176-4 doi (DE-627)OLC2066816043 (DE-He213)s10856-010-4176-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Wu, J. M. verfasserin aut Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2010 Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. Collagen matrix Basic fibroblast growth factor Heparin Crosslinking Controlled release Xu, Y. Y. aut Li, Z. H. aut Yuan, X. Y. aut Wang, P. F. aut Zhang, X. Z. aut Liu, Y. Q. aut Guan, J. aut Guo, Y. aut Li, R. X. aut Zhang, H. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2010), 1 vom: 04. Nov., Seite 107-114 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2010 number:1 day:04 month:11 pages:107-114 https://doi.org/10.1007/s10856-010-4176-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2010 1 04 11 107-114 |
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10.1007/s10856-010-4176-4 doi (DE-627)OLC2066816043 (DE-He213)s10856-010-4176-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Wu, J. M. verfasserin aut Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2010 Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. Collagen matrix Basic fibroblast growth factor Heparin Crosslinking Controlled release Xu, Y. Y. aut Li, Z. H. aut Yuan, X. Y. aut Wang, P. F. aut Zhang, X. Z. aut Liu, Y. Q. aut Guan, J. aut Guo, Y. aut Li, R. X. aut Zhang, H. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2010), 1 vom: 04. Nov., Seite 107-114 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2010 number:1 day:04 month:11 pages:107-114 https://doi.org/10.1007/s10856-010-4176-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2010 1 04 11 107-114 |
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10.1007/s10856-010-4176-4 doi (DE-627)OLC2066816043 (DE-He213)s10856-010-4176-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Wu, J. M. verfasserin aut Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2010 Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. Collagen matrix Basic fibroblast growth factor Heparin Crosslinking Controlled release Xu, Y. Y. aut Li, Z. H. aut Yuan, X. Y. aut Wang, P. F. aut Zhang, X. Z. aut Liu, Y. Q. aut Guan, J. aut Guo, Y. aut Li, R. X. aut Zhang, H. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2010), 1 vom: 04. Nov., Seite 107-114 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2010 number:1 day:04 month:11 pages:107-114 https://doi.org/10.1007/s10856-010-4176-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2010 1 04 11 107-114 |
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10.1007/s10856-010-4176-4 doi (DE-627)OLC2066816043 (DE-He213)s10856-010-4176-4-p DE-627 ger DE-627 rakwb eng 610 670 VZ Wu, J. M. verfasserin aut Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor 2010 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2010 Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. Collagen matrix Basic fibroblast growth factor Heparin Crosslinking Controlled release Xu, Y. Y. aut Li, Z. H. aut Yuan, X. Y. aut Wang, P. F. aut Zhang, X. Z. aut Liu, Y. Q. aut Guan, J. aut Guo, Y. aut Li, R. X. aut Zhang, H. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2010), 1 vom: 04. Nov., Seite 107-114 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2010 number:1 day:04 month:11 pages:107-114 https://doi.org/10.1007/s10856-010-4176-4 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2010 1 04 11 107-114 |
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Enthalten in Journal of materials science / Materials in medicine 22(2010), 1 vom: 04. Nov., Seite 107-114 volume:22 year:2010 number:1 day:04 month:11 pages:107-114 |
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Enthalten in Journal of materials science / Materials in medicine 22(2010), 1 vom: 04. Nov., Seite 107-114 volume:22 year:2010 number:1 day:04 month:11 pages:107-114 |
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Wu, J. M. @@aut@@ Xu, Y. Y. @@aut@@ Li, Z. H. @@aut@@ Yuan, X. Y. @@aut@@ Wang, P. F. @@aut@@ Zhang, X. Z. @@aut@@ Liu, Y. Q. @@aut@@ Guan, J. @@aut@@ Guo, Y. @@aut@@ Li, R. X. @@aut@@ Zhang, H. @@aut@@ |
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Wu, J. M. Xu, Y. Y. Li, Z. H. Yuan, X. Y. Wang, P. F. Zhang, X. Z. Liu, Y. Q. Guan, J. Guo, Y. Li, R. X. Zhang, H. |
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heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor |
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Heparin-functionalized collagen matrices with controlled release of basic fibroblast growth factor |
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Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. © Springer Science+Business Media, LLC 2010 |
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Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. © Springer Science+Business Media, LLC 2010 |
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Abstract Tissue engineering scaffolds with controlled long-term release of growth factors are constructed in an attempt to mimic the intelligent ability of the extracellular matrix (ECM) to release endogenous growth factors. In this study, collagen sponges (Collagen group) were modified by N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) crosslinking (EDC/NHS group) and heparin immobilization (EDC/NHS-H group), and subsequently seeded with human umbilical vein endothelial cells (HUVECs). Native and modified sponges were pre-adsorbed with basic fibroblast growth factor (bFGF) to evaluate the sustained release and bioactive maintenance of bFGF from the sponges. We found that modified collagen matrices permitted HUVECs to proliferate and migrate well and to distribute uniformly. The EDC/NHS-H group exhibited an excellent sustained-release profile and bioactive maintenance of the pre-adsorbed bFGF as compared with the Collagen and EDC/NHS groups. These results suggest that heparin-functionalized collagen matrices can support a controlled release of bFGF and thus, have potential as a tissue engineering scaffold. © Springer Science+Business Media, LLC 2010 |
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