The effect of substrate topography on hFOB cell behavior and initial cell adhesion evaluated by a cytodetacher
Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The groov...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yang, Shih-Ping [verfasserIn] |
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| Format: |
Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2011 |
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| Schlagwörter: |
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| Anmerkung: |
© Springer Science+Business Media, LLC 2011 |
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| Übergeordnetes Werk: |
Enthalten in: Journal of materials science / Materials in medicine - Springer US, 1990, 22(2011), 4 vom: 18. Feb., Seite 1027-1036 |
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| Übergeordnetes Werk: |
volume:22 ; year:2011 ; number:4 ; day:18 ; month:02 ; pages:1027-1036 |
| Links: |
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| DOI / URN: |
10.1007/s10856-011-4255-1 |
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OLC2066816981 |
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| 520 | |a Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. | ||
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10.1007/s10856-011-4255-1 doi (DE-627)OLC2066816981 (DE-He213)s10856-011-4255-1-p DE-627 ger DE-627 rakwb eng 610 670 VZ Yang, Shih-Ping verfasserin aut The effect of substrate topography on hFOB cell behavior and initial cell adhesion evaluated by a cytodetacher 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2011 Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. Adhesion Force Smooth Specimen Groove Surface Elongation Index Groove Pattern Lee, Tzer-Min aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2011), 4 vom: 18. Feb., Seite 1027-1036 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2011 number:4 day:18 month:02 pages:1027-1036 https://doi.org/10.1007/s10856-011-4255-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2011 4 18 02 1027-1036 |
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10.1007/s10856-011-4255-1 doi (DE-627)OLC2066816981 (DE-He213)s10856-011-4255-1-p DE-627 ger DE-627 rakwb eng 610 670 VZ Yang, Shih-Ping verfasserin aut The effect of substrate topography on hFOB cell behavior and initial cell adhesion evaluated by a cytodetacher 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2011 Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. Adhesion Force Smooth Specimen Groove Surface Elongation Index Groove Pattern Lee, Tzer-Min aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2011), 4 vom: 18. Feb., Seite 1027-1036 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2011 number:4 day:18 month:02 pages:1027-1036 https://doi.org/10.1007/s10856-011-4255-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2011 4 18 02 1027-1036 |
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10.1007/s10856-011-4255-1 doi (DE-627)OLC2066816981 (DE-He213)s10856-011-4255-1-p DE-627 ger DE-627 rakwb eng 610 670 VZ Yang, Shih-Ping verfasserin aut The effect of substrate topography on hFOB cell behavior and initial cell adhesion evaluated by a cytodetacher 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2011 Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. Adhesion Force Smooth Specimen Groove Surface Elongation Index Groove Pattern Lee, Tzer-Min aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2011), 4 vom: 18. Feb., Seite 1027-1036 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2011 number:4 day:18 month:02 pages:1027-1036 https://doi.org/10.1007/s10856-011-4255-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2011 4 18 02 1027-1036 |
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10.1007/s10856-011-4255-1 doi (DE-627)OLC2066816981 (DE-He213)s10856-011-4255-1-p DE-627 ger DE-627 rakwb eng 610 670 VZ Yang, Shih-Ping verfasserin aut The effect of substrate topography on hFOB cell behavior and initial cell adhesion evaluated by a cytodetacher 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2011 Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. Adhesion Force Smooth Specimen Groove Surface Elongation Index Groove Pattern Lee, Tzer-Min aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2011), 4 vom: 18. Feb., Seite 1027-1036 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2011 number:4 day:18 month:02 pages:1027-1036 https://doi.org/10.1007/s10856-011-4255-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2011 4 18 02 1027-1036 |
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10.1007/s10856-011-4255-1 doi (DE-627)OLC2066816981 (DE-He213)s10856-011-4255-1-p DE-627 ger DE-627 rakwb eng 610 670 VZ Yang, Shih-Ping verfasserin aut The effect of substrate topography on hFOB cell behavior and initial cell adhesion evaluated by a cytodetacher 2011 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media, LLC 2011 Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. Adhesion Force Smooth Specimen Groove Surface Elongation Index Groove Pattern Lee, Tzer-Min aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 22(2011), 4 vom: 18. Feb., Seite 1027-1036 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:22 year:2011 number:4 day:18 month:02 pages:1027-1036 https://doi.org/10.1007/s10856-011-4255-1 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_21 GBV_ILN_23 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2015 GBV_ILN_2021 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 22 2011 4 18 02 1027-1036 |
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the effect of substrate topography on hfob cell behavior and initial cell adhesion evaluated by a cytodetacher |
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The effect of substrate topography on hFOB cell behavior and initial cell adhesion evaluated by a cytodetacher |
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Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. © Springer Science+Business Media, LLC 2011 |
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Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. © Springer Science+Business Media, LLC 2011 |
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Abstract This study examined human fetal osteoblast (hFOB) cell morphology, adhesion force, and proliferation on a titanium-coated grooved surface. V-shaped grooves with a depth of 2.4 μm (T1) or 4.8 μm (T2) were produced in silicon wafers using photolithography and wet etching techniques. The grooved substrates were coated with a 200-nm-thick layer of titanium using a sputtering system. Smooth Ti-coated Si wafers were used as control surfaces. Analysis of the scanning electron microscopy observations shows that the cells responded to the micropattern by spreading out and becoming elongated. The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay indicated that the grooved specimens had a significantly larger number of cells than did the control group after 5- and 15-day cultures. The cytocompatibility of specimens was quantitatively evaluated by a cytodetacher, which directly measures the detachment shear force of an individual cell to the substrate. After 30-min culture, the cell adhesion forces were 48.4, 136.6, and 103.3 nN for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion strengths were 294, 501, and 590 Pa for the smooth specimen, the T1 specimen, and the T2 specimen, respectively. The cell adhesion force and cell adhesion strength indicate the quality of cell adhesion, explaining the largest number of cells on grooved specimens. The experimental results suggest that the grooved patterns affect the cell shape and cytoskeletal structure, and thus influence the cell proliferation and cell adhesion force. The cytodetachment test with nanonewton resolution is a sensitive method for studying cell–biomaterial interaction. © Springer Science+Business Media, LLC 2011 |
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