Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity
Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in sil...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Paladini, F. [verfasserIn] |
|---|
| Format: |
Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2013 |
|---|
| Schlagwörter: |
|---|
| Anmerkung: |
© Springer Science+Business Media New York 2013 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Journal of materials science / Materials in medicine - Springer US, 1990, 24(2013), 4 vom: 31. Jan., Seite 1105-1112 |
|---|---|
| Übergeordnetes Werk: |
volume:24 ; year:2013 ; number:4 ; day:31 ; month:01 ; pages:1105-1112 |
| Links: |
|---|
| DOI / URN: |
10.1007/s10856-013-4870-0 |
|---|
| Katalog-ID: |
OLC2066822140 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | OLC2066822140 | ||
| 003 | DE-627 | ||
| 005 | 20230518074053.0 | ||
| 007 | tu | ||
| 008 | 200819s2013 xx ||||| 00| ||eng c | ||
| 024 | 7 | |a 10.1007/s10856-013-4870-0 |2 doi | |
| 035 | |a (DE-627)OLC2066822140 | ||
| 035 | |a (DE-He213)s10856-013-4870-0-p | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | 4 | |a 610 |a 670 |q VZ |
| 100 | 1 | |a Paladini, F. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity |
| 264 | 1 | |c 2013 | |
| 336 | |a Text |b txt |2 rdacontent | ||
| 337 | |a ohne Hilfsmittel zu benutzen |b n |2 rdamedia | ||
| 338 | |a Band |b nc |2 rdacarrier | ||
| 500 | |a © Springer Science+Business Media New York 2013 | ||
| 520 | |a Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. | ||
| 650 | 4 | |a Silver Nanoparticles | |
| 650 | 4 | |a Silver Particle | |
| 650 | 4 | |a Silver Coating | |
| 650 | 4 | |a Bacterial Reduction | |
| 650 | 4 | |a Silver Sulfadiazine | |
| 700 | 1 | |a Pollini, M. |4 aut | |
| 700 | 1 | |a Deponti, D. |4 aut | |
| 700 | 1 | |a Di Giancamillo, A. |4 aut | |
| 700 | 1 | |a Peretti, G. |4 aut | |
| 700 | 1 | |a Sannino, A. |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Journal of materials science / Materials in medicine |d Springer US, 1990 |g 24(2013), 4 vom: 31. Jan., Seite 1105-1112 |w (DE-627)130865028 |w (DE-600)1031752-1 |w (DE-576)023107537 |x 0957-4530 |7 nnns |
| 773 | 1 | 8 | |g volume:24 |g year:2013 |g number:4 |g day:31 |g month:01 |g pages:1105-1112 |
| 856 | 4 | 1 | |u https://doi.org/10.1007/s10856-013-4870-0 |z lizenzpflichtig |3 Volltext |
| 912 | |a GBV_USEFLAG_A | ||
| 912 | |a SYSFLAG_A | ||
| 912 | |a GBV_OLC | ||
| 912 | |a SSG-OLC-TEC | ||
| 912 | |a SSG-OLC-PHA | ||
| 912 | |a SSG-OLC-DE-84 | ||
| 912 | |a GBV_ILN_32 | ||
| 912 | |a GBV_ILN_70 | ||
| 912 | |a GBV_ILN_2004 | ||
| 912 | |a GBV_ILN_2005 | ||
| 912 | |a GBV_ILN_4012 | ||
| 912 | |a GBV_ILN_4046 | ||
| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4219 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4323 | ||
| 951 | |a AR | ||
| 952 | |d 24 |j 2013 |e 4 |b 31 |c 01 |h 1105-1112 | ||
| author_variant |
f p fp m p mp d d dd g a d ga gad g p gp a s as |
|---|---|
| matchkey_str |
article:09574530:2013----::fetfivraootnsnahtrframdayiitrsfelibltpoieaino |
| hierarchy_sort_str |
2013 |
| publishDate |
2013 |
| allfields |
10.1007/s10856-013-4870-0 doi (DE-627)OLC2066822140 (DE-He213)s10856-013-4870-0-p DE-627 ger DE-627 rakwb eng 610 670 VZ Paladini, F. verfasserin aut Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2013 Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. Silver Nanoparticles Silver Particle Silver Coating Bacterial Reduction Silver Sulfadiazine Pollini, M. aut Deponti, D. aut Di Giancamillo, A. aut Peretti, G. aut Sannino, A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 24(2013), 4 vom: 31. Jan., Seite 1105-1112 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:24 year:2013 number:4 day:31 month:01 pages:1105-1112 https://doi.org/10.1007/s10856-013-4870-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 24 2013 4 31 01 1105-1112 |
| spelling |
10.1007/s10856-013-4870-0 doi (DE-627)OLC2066822140 (DE-He213)s10856-013-4870-0-p DE-627 ger DE-627 rakwb eng 610 670 VZ Paladini, F. verfasserin aut Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2013 Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. Silver Nanoparticles Silver Particle Silver Coating Bacterial Reduction Silver Sulfadiazine Pollini, M. aut Deponti, D. aut Di Giancamillo, A. aut Peretti, G. aut Sannino, A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 24(2013), 4 vom: 31. Jan., Seite 1105-1112 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:24 year:2013 number:4 day:31 month:01 pages:1105-1112 https://doi.org/10.1007/s10856-013-4870-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 24 2013 4 31 01 1105-1112 |
| allfields_unstemmed |
10.1007/s10856-013-4870-0 doi (DE-627)OLC2066822140 (DE-He213)s10856-013-4870-0-p DE-627 ger DE-627 rakwb eng 610 670 VZ Paladini, F. verfasserin aut Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2013 Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. Silver Nanoparticles Silver Particle Silver Coating Bacterial Reduction Silver Sulfadiazine Pollini, M. aut Deponti, D. aut Di Giancamillo, A. aut Peretti, G. aut Sannino, A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 24(2013), 4 vom: 31. Jan., Seite 1105-1112 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:24 year:2013 number:4 day:31 month:01 pages:1105-1112 https://doi.org/10.1007/s10856-013-4870-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 24 2013 4 31 01 1105-1112 |
| allfieldsGer |
10.1007/s10856-013-4870-0 doi (DE-627)OLC2066822140 (DE-He213)s10856-013-4870-0-p DE-627 ger DE-627 rakwb eng 610 670 VZ Paladini, F. verfasserin aut Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2013 Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. Silver Nanoparticles Silver Particle Silver Coating Bacterial Reduction Silver Sulfadiazine Pollini, M. aut Deponti, D. aut Di Giancamillo, A. aut Peretti, G. aut Sannino, A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 24(2013), 4 vom: 31. Jan., Seite 1105-1112 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:24 year:2013 number:4 day:31 month:01 pages:1105-1112 https://doi.org/10.1007/s10856-013-4870-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 24 2013 4 31 01 1105-1112 |
| allfieldsSound |
10.1007/s10856-013-4870-0 doi (DE-627)OLC2066822140 (DE-He213)s10856-013-4870-0-p DE-627 ger DE-627 rakwb eng 610 670 VZ Paladini, F. verfasserin aut Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity 2013 Text txt rdacontent ohne Hilfsmittel zu benutzen n rdamedia Band nc rdacarrier © Springer Science+Business Media New York 2013 Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. Silver Nanoparticles Silver Particle Silver Coating Bacterial Reduction Silver Sulfadiazine Pollini, M. aut Deponti, D. aut Di Giancamillo, A. aut Peretti, G. aut Sannino, A. aut Enthalten in Journal of materials science / Materials in medicine Springer US, 1990 24(2013), 4 vom: 31. Jan., Seite 1105-1112 (DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 0957-4530 nnns volume:24 year:2013 number:4 day:31 month:01 pages:1105-1112 https://doi.org/10.1007/s10856-013-4870-0 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 AR 24 2013 4 31 01 1105-1112 |
| language |
English |
| source |
Enthalten in Journal of materials science / Materials in medicine 24(2013), 4 vom: 31. Jan., Seite 1105-1112 volume:24 year:2013 number:4 day:31 month:01 pages:1105-1112 |
| sourceStr |
Enthalten in Journal of materials science / Materials in medicine 24(2013), 4 vom: 31. Jan., Seite 1105-1112 volume:24 year:2013 number:4 day:31 month:01 pages:1105-1112 |
| format_phy_str_mv |
Article |
| institution |
findex.gbv.de |
| topic_facet |
Silver Nanoparticles Silver Particle Silver Coating Bacterial Reduction Silver Sulfadiazine |
| dewey-raw |
610 |
| isfreeaccess_bool |
false |
| container_title |
Journal of materials science / Materials in medicine |
| authorswithroles_txt_mv |
Paladini, F. @@aut@@ Pollini, M. @@aut@@ Deponti, D. @@aut@@ Di Giancamillo, A. @@aut@@ Peretti, G. @@aut@@ Sannino, A. @@aut@@ |
| publishDateDaySort_date |
2013-01-31T00:00:00Z |
| hierarchy_top_id |
130865028 |
| dewey-sort |
3610 |
| id |
OLC2066822140 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">OLC2066822140</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230518074053.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">200819s2013 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s10856-013-4870-0</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2066822140</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s10856-013-4870-0-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="a">670</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Paladini, F.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2013</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer Science+Business Media New York 2013</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver Nanoparticles</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver Particle</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver Coating</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacterial Reduction</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver Sulfadiazine</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pollini, M.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Deponti, D.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Di Giancamillo, A.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Peretti, G.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sannino, A.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of materials science / Materials in medicine</subfield><subfield code="d">Springer US, 1990</subfield><subfield code="g">24(2013), 4 vom: 31. Jan., Seite 1105-1112</subfield><subfield code="w">(DE-627)130865028</subfield><subfield code="w">(DE-600)1031752-1</subfield><subfield code="w">(DE-576)023107537</subfield><subfield code="x">0957-4530</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:24</subfield><subfield code="g">year:2013</subfield><subfield code="g">number:4</subfield><subfield code="g">day:31</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:1105-1112</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s10856-013-4870-0</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4219</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">24</subfield><subfield code="j">2013</subfield><subfield code="e">4</subfield><subfield code="b">31</subfield><subfield code="c">01</subfield><subfield code="h">1105-1112</subfield></datafield></record></collection>
|
| author |
Paladini, F. |
| spellingShingle |
Paladini, F. ddc 610 misc Silver Nanoparticles misc Silver Particle misc Silver Coating misc Bacterial Reduction misc Silver Sulfadiazine Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity |
| authorStr |
Paladini, F. |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)130865028 |
| format |
Article |
| dewey-ones |
610 - Medicine & health 670 - Manufacturing |
| delete_txt_mv |
keep |
| author_role |
aut aut aut aut aut aut |
| collection |
OLC |
| remote_str |
false |
| illustrated |
Not Illustrated |
| issn |
0957-4530 |
| topic_title |
610 670 VZ Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity Silver Nanoparticles Silver Particle Silver Coating Bacterial Reduction Silver Sulfadiazine |
| topic |
ddc 610 misc Silver Nanoparticles misc Silver Particle misc Silver Coating misc Bacterial Reduction misc Silver Sulfadiazine |
| topic_unstemmed |
ddc 610 misc Silver Nanoparticles misc Silver Particle misc Silver Coating misc Bacterial Reduction misc Silver Sulfadiazine |
| topic_browse |
ddc 610 misc Silver Nanoparticles misc Silver Particle misc Silver Coating misc Bacterial Reduction misc Silver Sulfadiazine |
| format_facet |
Aufsätze Gedruckte Aufsätze |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
nc |
| hierarchy_parent_title |
Journal of materials science / Materials in medicine |
| hierarchy_parent_id |
130865028 |
| dewey-tens |
610 - Medicine & health 670 - Manufacturing |
| hierarchy_top_title |
Journal of materials science / Materials in medicine |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)130865028 (DE-600)1031752-1 (DE-576)023107537 |
| title |
Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity |
| ctrlnum |
(DE-627)OLC2066822140 (DE-He213)s10856-013-4870-0-p |
| title_full |
Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity |
| author_sort |
Paladini, F. |
| journal |
Journal of materials science / Materials in medicine |
| journalStr |
Journal of materials science / Materials in medicine |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
600 - Technology |
| recordtype |
marc |
| publishDateSort |
2013 |
| contenttype_str_mv |
txt |
| container_start_page |
1105 |
| author_browse |
Paladini, F. Pollini, M. Deponti, D. Di Giancamillo, A. Peretti, G. Sannino, A. |
| container_volume |
24 |
| class |
610 670 VZ |
| format_se |
Aufsätze |
| author-letter |
Paladini, F. |
| doi_str_mv |
10.1007/s10856-013-4870-0 |
| dewey-full |
610 670 |
| title_sort |
effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity |
| title_auth |
Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity |
| abstract |
Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. © Springer Science+Business Media New York 2013 |
| abstractGer |
Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. © Springer Science+Business Media New York 2013 |
| abstract_unstemmed |
Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs. © Springer Science+Business Media New York 2013 |
| collection_details |
GBV_USEFLAG_A SYSFLAG_A GBV_OLC SSG-OLC-TEC SSG-OLC-PHA SSG-OLC-DE-84 GBV_ILN_32 GBV_ILN_70 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_4012 GBV_ILN_4046 GBV_ILN_4125 GBV_ILN_4219 GBV_ILN_4305 GBV_ILN_4323 |
| container_issue |
4 |
| title_short |
Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity |
| url |
https://doi.org/10.1007/s10856-013-4870-0 |
| remote_bool |
false |
| author2 |
Pollini, M. Deponti, D. Di Giancamillo, A. Peretti, G. Sannino, A. |
| author2Str |
Pollini, M. Deponti, D. Di Giancamillo, A. Peretti, G. Sannino, A. |
| ppnlink |
130865028 |
| mediatype_str_mv |
n |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| doi_str |
10.1007/s10856-013-4870-0 |
| up_date |
2024-07-04T05:24:13.211Z |
| _version_ |
1803624805255086080 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">OLC2066822140</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230518074053.0</controlfield><controlfield tag="007">tu</controlfield><controlfield tag="008">200819s2013 xx ||||| 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s10856-013-4870-0</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)OLC2066822140</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-He213)s10856-013-4870-0-p</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="a">670</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Paladini, F.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Effect of silver nanocoatings on catheters for haemodialysis in terms of cell viability, proliferation, morphology and antibacterial activity</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2013</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">ohne Hilfsmittel zu benutzen</subfield><subfield code="b">n</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Band</subfield><subfield code="b">nc</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Springer Science+Business Media New York 2013</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The onset of infections associated to bacterial proliferation and biofilm formation on indwelling medical devices represents the major risk of morbidity and mortality among patients. In order to contain the risk of infections in clinical practice, there is a growing interest nowadays in silver-based products due to the strong antimicrobial efficacy of silver against a broad spectrum of microorganisms. In this work, temporary catheters for haemodialysis were coated with silver nano-particles through the in situ photo-reduction of a silver salt in alcoholic solution. A homogeneous distribution of silver particles firmly bonded to the substrate was obtained through the adopted technique. An optimisation study was required to define the amount of silver, in order to obtain good efficacy against Gram-positive and Gram-negative bacteria and no cytotoxic effect. At this purpose, three concentrations of silver, 0.1, 0.25 and 0.5 wt%, have been deposited and tested with respect to bacterial reduction percentage and cellular response. Particularly, bacterial enumeration on Escherichia coli and Staphylococcus aureus, and BrdU incorporation, TUNEL assay and Actin staining on a selected primary cell population were performed on catheters treated with the different silver solutions. The silver percentages tested demonstrated strong antibacterial properties together with a good cellular response, thus indicating that the developed product could be proposed in clinical practice and that the lower percentage tested can be preferred with evident advantages in terms of costs.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver Nanoparticles</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver Particle</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver Coating</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bacterial Reduction</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver Sulfadiazine</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pollini, M.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Deponti, D.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Di Giancamillo, A.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Peretti, G.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sannino, A.</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Journal of materials science / Materials in medicine</subfield><subfield code="d">Springer US, 1990</subfield><subfield code="g">24(2013), 4 vom: 31. Jan., Seite 1105-1112</subfield><subfield code="w">(DE-627)130865028</subfield><subfield code="w">(DE-600)1031752-1</subfield><subfield code="w">(DE-576)023107537</subfield><subfield code="x">0957-4530</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:24</subfield><subfield code="g">year:2013</subfield><subfield code="g">number:4</subfield><subfield code="g">day:31</subfield><subfield code="g">month:01</subfield><subfield code="g">pages:1105-1112</subfield></datafield><datafield tag="856" ind1="4" ind2="1"><subfield code="u">https://doi.org/10.1007/s10856-013-4870-0</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_OLC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-TEC</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-DE-84</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4219</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">24</subfield><subfield code="j">2013</subfield><subfield code="e">4</subfield><subfield code="b">31</subfield><subfield code="c">01</subfield><subfield code="h">1105-1112</subfield></datafield></record></collection>
|
| score |
7.3978004 |